BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0167.p (250 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309665|ref|YP_003773757.1| 2,4-dihydroxyhept-2-ene-1,7-dio... 496 e-142 >gi|300309665|ref|YP_003773757.1| 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase [Herbaspirillum seropedicae SmR1] Length = 250 Score = 496 bits (1278), Expect = e-142, Method: Compositional matrix adjust. Identities = 240/250 (96%), Positives = 247/250 (98%) Query: 1 MRENRLRTLFAQQQLALSGWVSMGNSLLAEVLGHSGYDAVTVDLQHGPFSIESALPMLQA 60 MRENRLRTLFAQQQLALSGWVSMGN+LLAEV+ HSGYDAVTVDLQHGPFSI++ALPMLQA Sbjct: 1 MRENRLRTLFAQQQLALSGWVSMGNALLAEVIAHSGYDAVTVDLQHGPFSIDAALPMLQA 60 Query: 61 ISSTPAVPLVRCSENSLGEINKLLDAGAYGVICPLINTAADAERFVRACHYSPRGGRSYG 120 ISSTPAVPLVRCSENSLGEINKLLDAGAYGVICPLINTAADAERFVRACHYSPRGGRSYG Sbjct: 61 ISSTPAVPLVRCSENSLGEINKLLDAGAYGVICPLINTAADAERFVRACHYSPRGGRSYG 120 Query: 121 PARGFLYGGADYFAHANATILTLAMIETREGYANAEAILQTPDLDGIFIGPSDLAIELGL 180 PARGFLYGGADYF HANAT+LTLAMIETREGYANAEAILQTPDLDGIFIGPSDLAIELGL Sbjct: 121 PARGFLYGGADYFTHANATLLTLAMIETREGYANAEAILQTPDLDGIFIGPSDLAIELGL 180 Query: 181 APDAYDDARLNEAIDHLLALARKLGKYVGIFAGTMEMAQRMKGIGMDLVVPGTDIQQIKA 240 APDAYDD RLNEAIDHLLALARKLGKYVGIFAGTMEMAQRMKGIGMDLVVPGTDIQQIKA Sbjct: 181 APDAYDDVRLNEAIDHLLALARKLGKYVGIFAGTMEMAQRMKGIGMDLVVPGTDIQQIKA 240 Query: 241 EGARRIAALR 250 EGARR++ALR Sbjct: 241 EGARRVSALR 250 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 824,431 Number of extensions: 35419 Number of successful extensions: 114 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 114 Number of HSP's successfully gapped: 1 Length of query: 250 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 167 Effective length of database: 1,226,865 Effective search space: 204886455 Effective search space used: 204886455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)