BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0164.p (335 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 680 0.0 gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Her... 153 2e-38 gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 142 3e-35 gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbasp... 138 8e-34 gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Her... 99 5e-22 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 680 bits (1754), Expect = 0.0, Method: Compositional matrix adjust. Identities = 323/335 (96%), Positives = 332/335 (99%) Query: 1 MHYRRLGKSNLKVSALCLGSMMFGDQTDLEESGRIVASAREHGINFIDTADVYTKGKSEE 60 MHYRRLGKSNLKVSALCLGSMMFGDQTDL+ESGRIVASAREHG+NFIDTADVYTKGKSEE Sbjct: 1 MHYRRLGKSNLKVSALCLGSMMFGDQTDLQESGRIVASAREHGVNFIDTADVYTKGKSEE 60 Query: 61 IVGKLLAGQRHEWVLASKLGNAMSGKPNESHYSRSWIVRETENILKRLDTDYLDILYMHR 120 IVGKLLAGQRHEWVLASKLGNAMSGKPNES+YSRSWIVRETENIL RLDTDYLDILYMHR Sbjct: 61 IVGKLLAGQRHEWVLASKLGNAMSGKPNESNYSRSWIVRETENILTRLDTDYLDILYMHR 120 Query: 121 DYQDENLEEAVQALGDLIRAGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPLVCQPYYNL 180 DYQDENLEEAVQALGDLIR GKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPLVCQPYYNL Sbjct: 121 DYQDENLEEAVQALGDLIRVGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPLVCQPYYNL 180 Query: 181 LNRQPEVEILPACANYGLGVVPYSPIARGVLTGKYQPGQKPPQDTRAGRGDKRMMETEFR 240 LNRQPEVEILPACA+YGLGVVPYSPIARGVLTGKYQPGQKP QDTRAGRGDKRMMETEFR Sbjct: 181 LNRQPEVEILPACAHYGLGVVPYSPIARGVLTGKYQPGQKPAQDTRAGRGDKRMMETEFR 240 Query: 241 EESLLIAQRLQQHCEARGIKLGQFATAWVLANASISSVIAGPRTLAQMEDYYDAAEMKIN 300 EESLLIAQRLQQHCEARG+KLGQFATAWVLAN++ISSVIAGPRTLAQMEDYYDAAE+KI+ Sbjct: 241 EESLLIAQRLQQHCEARGVKLGQFATAWVLANSAISSVIAGPRTLAQMEDYYDAAELKIS 300 Query: 301 AEEEALVDSLVTPGHASTHGYNDPNYPFFGRVVRG 335 AEEEALVDSLVTPGHASTHGYNDPNYPFFGRVVRG Sbjct: 301 AEEEALVDSLVTPGHASTHGYNDPNYPFFGRVVRG 335 >gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 344 Score = 153 bits (387), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 112/324 (34%), Positives = 166/324 (51%), Gaps = 15/324 (4%) Query: 1 MHYRRLGKSNLKVSALCLGSMM-FGDQTDLEESGRIVASAREHGINFIDTADVY--TKGK 57 M YRR G+S L + A+ LG FG + E ++ A + G+ D A+ Y G Sbjct: 14 MPYRRTGRSGLLLPAISLGLWQNFGGVDNFETGRAVLRRAFDRGVTHFDLANNYGPPAGS 73 Query: 58 SEEIVGKLLAGQ----RHEWVLASKLGNAMSGKP-NESHYSRSWIVRETENILKRLDTDY 112 +EE G+ +A R E V++SK G M P +R ++ E LKR+ DY Sbjct: 74 AEENFGRWMAKDFLPYRDELVISSKAGWQMWPGPYGGPSGARKHLIASCEQSLKRMGLDY 133 Query: 113 LDILYMHRDYQDENLEEAVQALGDLIRAGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPL 172 +DI Y HR D LEE + AL L R GK G+S++ + + + VPL L Sbjct: 134 VDIFYSHRVDADTPLEETMGALAHLHRQGKALYVGISSYSPELTRKAAAILKSEGVPL-L 192 Query: 173 VCQPYYNLLNRQPEVEILPACANYGLGVVPYSPIARGVLTGKYQPG-QKPPQDTRAGRGD 231 + QP Y++LNR E +LPA G+G + +SP+A+G+LT KY G + + TRA Sbjct: 193 IHQPNYSMLNRAIEGGLLPALEELGVGCIGFSPLAQGLLTSKYLGGVPEGARVTRAPSLP 252 Query: 232 KRMMETEFREESLLIAQRLQQHCEARGIKLGQFATAWVLANASISSVIAGPRTLAQMEDY 291 +RM+ E++L + L + RG L Q A AWVL +A ++S + G RT+ Q++D Sbjct: 253 QRMLS----EDNLARIRALNEIASKRGQTLAQMAIAWVLRDARVTSALIGARTVEQLDDS 308 Query: 292 YDAA-EMKINAEEEALVDSLVTPG 314 DA ++ EE A +D T G Sbjct: 309 LDAVNKLDFTQEELASIDKHATDG 332 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 142 bits (359), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 106/332 (31%), Positives = 161/332 (48%), Gaps = 26/332 (7%) Query: 4 RRLGKSNLKVSALCLGSMMFGD---QTDLEESGRIVASAREHGINFIDTADVYTKGKSEE 60 RRLG + VSA+ LG M D Q D ES + A E GIN +DTAD+Y +EE Sbjct: 18 RRLGSNGPLVSAIGLGCMGMSDFYAQRDDAESLATIDRALELGINLLDTADMYGPYTNEE 77 Query: 61 IVGKLLAGQRHEWVLASKLGNAMSGKPNESHY-----SRSWIVRETENILKRLDTDYLDI 115 ++G+ + G+R ++ +A+K G + P++ S ++I + E LKRL + +D+ Sbjct: 78 LLGRAIKGRRDKFFIATKFG--IRRDPSDPGARGVDGSPAYIRQAVEGSLKRLGVETIDL 135 Query: 116 LYMHRDYQDENLEEAVQALGDLIRAGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPLVCQ 175 Y HR +E + L DL++AGKIR G+S A L + V Q Sbjct: 136 YYQHRIDPATPIEVTMGVLADLVQAGKIRYIGLSE------ASAATLERAHRVHPVTALQ 189 Query: 176 PYYNLLNRQPEVEILPACANYGLGVVPYSPIARGVLTGKYQPGQKPPQDTRAGRGDKRMM 235 Y+L R PE E+L AC G+G V YSP+ RG LTG Q + +D D R Sbjct: 190 SEYSLWTRDPEAEVLAACRTLGIGFVAYSPLGRGFLTGAIQRFEDLAED------DFRRS 243 Query: 236 ETEFREE----SLLIAQRLQQHCEARGIKLGQFATAWVLANASISSVIAGPRTLAQMEDY 291 F+ E +L +A+++++ G Q A AWV+A I G + +ED Sbjct: 244 NPRFQGENFARNLQLAEKVKEMAGQHGCTPSQLALAWVMAQDPHIVPIPGTKRRRYLEDN 303 Query: 292 YDAAEMKINAEEEALVDSLVTPGHASTHGYND 323 + +K+ E+ +D++ G A+ Y D Sbjct: 304 AGSVGVKLAPEDLQALDAIFPRGAAAGERYTD 335 >gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 349 Score = 138 bits (347), Expect = 8e-34, Method: Compositional matrix adjust. Identities = 99/347 (28%), Positives = 170/347 (48%), Gaps = 44/347 (12%) Query: 1 MHYRRLGKSNLKVSALCLGSMMFGDQTDLEESGRIVASAREHGINFIDTADVY------- 53 M YR LG+++LKVS + LG+M +G+Q ++ + A E GIN ID A++Y Sbjct: 1 MQYRPLGRTDLKVSLITLGTMTWGEQNSEADAHAQLDYAVERGINLIDVAEMYPVPPKAE 60 Query: 54 TKGKSEEIVGKLL--AGQRHEWVLASKLGNAMSGKPNESH-------YSRSWIVRETENI 104 T+G +E +G L +G+R + ++A+K N H R + Sbjct: 61 TQGLTERYLGTWLKKSGKRDQLLIATKCTGPARKPHNPRHVRGGINNLDRKGLTEALHGS 120 Query: 105 LKRLDTDYLDILYMH--------------RDYQDEN---LEEAVQALGDLIRAGKIRGFG 147 L+RL D++D+ +H + +DE+ +EE + L + ++AGKIR G Sbjct: 121 LERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVKAGKIRTIG 180 Query: 148 VSNFRGWRIAEIVRLCEKNNVPLPLVCQPYYNLLNRQPEVEILPACANYGLGVVPYSPIA 207 +SN W +A+ +R E++ + + Q YNLLNR E+ + +G++ YSP+A Sbjct: 181 LSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVGLLAYSPLA 240 Query: 208 RGVLTGKYQPGQKPPQDTRAGRGDKRMMETEFREESLLIAQRLQQHCEA----RGIKLGQ 263 G+L+GKY G +P G + + F + A+R A G+ Q Sbjct: 241 FGMLSGKYLDGARPA-------GGRLTLFERFSRYTNAQAERATAEYVALARQHGLDPAQ 293 Query: 264 FATAWVLANASISSVIAGPRTLAQMEDYYDAAEMKINAEEEALVDSL 310 A A+V + ++S I G TLAQ+ D+AE+++ + A ++++ Sbjct: 294 MALAYVNSRPFLTSNIIGATTLAQLRSNIDSAELQLGEDVLAAIEAI 340 >gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 329 Score = 99.0 bits (245), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 88/316 (27%), Positives = 147/316 (46%), Gaps = 22/316 (6%) Query: 1 MHYRRLGKSNLKVSALCLGSMMFGDQ---TDLEESGRIVASAREHGINFIDTADVYTKGK 57 M RR G++ +VS + G+ G +++ + +A + G++FIDTADVY G+ Sbjct: 1 MKSRRFGRTGAQVSEIGFGAWAIGGSWGDVSAQDAKAALHAALDAGVSFIDTADVYGDGR 60 Query: 58 SEEIVGKLL---AGQRHEWVLASKLGNAMSGKPNESHYSRSWIVRETENILKRLDTDYLD 114 SE+++ ++L G+R +A+K G +S + R+ + + LK L D LD Sbjct: 61 SEQLIAQVLKERGGERP--FVATKAGRRLSPHVASGYNDRAQLSAFIDRSLKNLGVDCLD 118 Query: 115 ILYMHRDYQDENLEEAVQA-LGDLIRAGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPLV 173 ++ +H + ++ V L L+ AGKIR +GVS ++ E ++ E NV Sbjct: 119 LVQLHCPPTEVYYQQEVFGHLDALVAAGKIRHYGVSV---EKVEEALKAIEYPNVK---S 172 Query: 174 CQPYYNLLNRQPEVEILPACANYGLGVVPYSPIARGVLTGKYQPGQKPPQD-----TRAG 228 Q +N+ ++P L + V+ P+A G+LTGK D R G Sbjct: 173 VQIIFNIFRQRPISLFLQEAQRRDIAVIARVPLASGLLTGKMSAQTTFAADDHRQFNRHG 232 Query: 229 RG-DKRMMETEFREESLLIAQRLQQHCEARGIKLGQFATAWVLANASISSVIAGPRTLAQ 287 D + E L A + +G+ + QFA W+L + IS+VI G R +AQ Sbjct: 233 EAFDVGETFSGVPYEVALAAVEKIRALVPQGVPMAQFALRWILMESGISTVIPGARNVAQ 292 Query: 288 MEDYYDAAEM-KINAE 302 + A+ + I AE Sbjct: 293 AQSNSAASALPPITAE 308 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.136 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,087,587 Number of extensions: 45326 Number of successful extensions: 137 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 124 Number of HSP's successfully gapped: 6 Length of query: 335 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 249 Effective length of database: 1,212,660 Effective search space: 301952340 Effective search space used: 301952340 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)