BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0022.0160.p
         (332 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309659|ref|YP_003773751.1| esterase-lipase superfamily pro...   530   e-152
gi|300311178|ref|YP_003775270.1| lipase [Herbaspirillum seropedi...   112   3e-26
gi|300311659|ref|YP_003775751.1| esterase/lipase [Herbaspirillum...   102   4e-23

>gi|300309659|ref|YP_003773751.1| esterase-lipase superfamily
           protein [Herbaspirillum seropedicae SmR1]
          Length = 332

 Score =  530 bits (1366), Expect = e-152,   Method: Compositional matrix adjust.
 Identities = 285/330 (86%), Positives = 299/330 (90%)

Query: 1   VNELISLIVQTYRTVLLDYRCLFETLLWRTIRSILGQVASAQALIMQGSSPLDIRNFFVP 60
           + E I+ IV+  RTVLLDYRCLF  LLWRTIRSILG VA AQA IMQGSS LDIR+FFVP
Sbjct: 1   MKEFITAIVRRNRTVLLDYRCLFRPLLWRTIRSILGPVAHAQAHIMQGSSSLDIRDFFVP 60

Query: 61  GPLGRIPVRLYQPAAALPKGEKRPLVLYFHGGGFTSGSLDDADTPASFLAEHNQAVVLSV 120
           GPLGRIPVRLY PA  L +G+KRPLV+YFHGGGF SGSL+DADTPA F+AEH Q  VLSV
Sbjct: 61  GPLGRIPVRLYLPATPLARGQKRPLVMYFHGGGFVSGSLEDADTPARFIAEHGQLAVLSV 120

Query: 121 GYALAPAKPFPAAPEDAFAAATWACKNATELQVDLDRMVVAGDDAGGSLAASLTMMTRDR 180
           GYALAPAKPFPAAPEDAFAAATWACKNATEL+VDLDRMVVAGDDAGG LAASLT+MTRDR
Sbjct: 121 GYALAPAKPFPAAPEDAFAAATWACKNATELKVDLDRMVVAGDDAGGCLAASLTLMTRDR 180

Query: 181 CGKPLAAQVLISPMLDPSMRLLGDATRLQSDVTAETCAASYRQYLPQTMQRLHPYAAPLE 240
           CGKPLAAQVLISPMLDPSMRLLGDA RLQSD+TAETCAASYRQYLPQTMQRLHPYAAPLE
Sbjct: 181 CGKPLAAQVLISPMLDPSMRLLGDAARLQSDLTAETCAASYRQYLPQTMQRLHPYAAPLE 240

Query: 241 VSRLAGLPPAFIATAERDVLCPEAEKYASSLIRAGVPTQVSRFVGITHGALRTHLPLLTE 300
           VSRLAGLPPAFIATAERDVLCPEAEKYASSLIRAGVPTQVSRF GITH ALRTHLPLLTE
Sbjct: 241 VSRLAGLPPAFIATAERDVLCPEAEKYASSLIRAGVPTQVSRFAGITHAALRTHLPLLTE 300

Query: 301 IVNFLRCRFATLRRDAALPEFLPHPLHPST 330
           IVNFLRCRFA L+  A  PEFLP+ L PST
Sbjct: 301 IVNFLRCRFAKLQGHAPHPEFLPNKLQPST 330


>gi|300311178|ref|YP_003775270.1| lipase [Herbaspirillum seropedicae
           SmR1]
          Length = 340

 Score =  112 bits (281), Expect = 3e-26,   Method: Compositional matrix adjust.
 Identities = 69/228 (30%), Positives = 113/228 (49%), Gaps = 7/228 (3%)

Query: 65  RIPVRLYQPAAALPKGEKRPLVLYFHGGGFTSGSLDDADTPASFLAEHNQAVVLSVGYAL 124
           +I + + +PA +  K    P  ++FHGGG+  G     +     L  ++ AV + V Y  
Sbjct: 86  QIKLTIVRPAGSAKK--TLPAFMFFHGGGWVLGDFPTHERLVRDLVANSGAVAVFVNYTP 143

Query: 125 APAKPFPAAPEDAFAAATWACKNATELQVDLDRMVVAGDDAGGSLAASLTMMTRDRCGKP 184
           +P   +  A   A+AA  W  ++  E+ VD  R+ VAG+  GG++AA + +M +D+ G  
Sbjct: 144 SPEAHYGVAINQAYAATKWVAEHGKEINVDGKRLAVAGNSVGGNMAAVVALMAKDKGGPA 203

Query: 185 LAAQVLISPMLDPSMRLLGDATRLQSD--VTAETCAASYRQYLPQTMQRLHPYAAPLEVS 242
           L ++VL+ P+ D +        +  +   +T       +  Y     +R    A+PL  +
Sbjct: 204 LRSEVLLWPVTDANFE-TASYNQFANGYFLTKNMMKWFWDSYTTDAAKRKEITASPLNAT 262

Query: 243 --RLAGLPPAFIATAERDVLCPEAEKYASSLIRAGVPTQVSRFVGITH 288
             +L+GLPP  I TAE+DVL  E E+Y   L  AGV     R+ G+ H
Sbjct: 263 PAQLSGLPPTLIQTAEKDVLRDEGEEYGRKLAAAGVAVTSVRYNGMIH 310


>gi|300311659|ref|YP_003775751.1| esterase/lipase [Herbaspirillum
           seropedicae SmR1]
          Length = 317

 Score =  102 bits (254), Expect = 4e-23,   Method: Compositional matrix adjust.
 Identities = 86/231 (37%), Positives = 114/231 (49%), Gaps = 7/231 (3%)

Query: 65  RIPVRLYQPAAALPKGEKRPLVLYFHGGGFTSGSLDDADTPASFLAEHNQAVVLSVGYAL 124
           RI  RLY PA        +P++LYFHGGG+  GSLD  DT  S LA      VL+V Y L
Sbjct: 67  RIGARLYAPAPLDAA-APQPVLLYFHGGGYCVGSLDSHDTLCSALAARTPCCVLAVDYRL 125

Query: 125 APAKPFPAAPEDAFAAATWACKNATELQVDLDRMVVAGDDAGGSLAASLTMMTRDRCGKP 184
           AP   FP A EDA  A  W  ++   L +  +R+ V GD AGG+LAA+L +  R     P
Sbjct: 126 APEHRFPTAFEDAADAYDWLLQHGPALGLATNRLAVGGDSAGGTLAAALALRARQAARAP 185

Query: 185 LAAQVLISPMLDPSMRLLGDATRLQSD---VTAETCAASYRQYLPQTMQRLHPYAAPLEV 241
              QVL+ P    S    GD+ R  +    +  ET    +   L    +R     APL+ 
Sbjct: 186 -CLQVLLYPCT--SAHQDGDSHRRFATGYLLEGETLQWMFGHTLNGDHERQDWRFAPLQA 242

Query: 242 SRLAGLPPAFIATAERDVLCPEAEKYASSLIRAGVPTQVSRFVGITHGALR 292
           + ++G  PA +   + D L  E   YA  L +AGVP Q+    G+ H  LR
Sbjct: 243 ADVSGAAPALVVIGDHDPLLDEGMAYAEKLRQAGVPVQLDIHPGMVHDFLR 293


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.322    0.136    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 925,540
Number of extensions: 36984
Number of successful extensions: 94
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 94
Number of HSP's successfully gapped: 3
Length of query: 332
Length of database: 1,619,870
Length adjustment: 86
Effective length of query: 246
Effective length of database: 1,212,660
Effective search space: 298314360
Effective search space used: 298314360
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 131 (55.1 bits)