BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0156.p (354 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 535 e-153 gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspiri... 249 2e-67 gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprot... 191 1e-49 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 186 3e-48 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 181 8e-47 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 535 bits (1377), Expect = e-153, Method: Compositional matrix adjust. Identities = 291/303 (96%), Positives = 298/303 (98%) Query: 1 VNVSYEVDLFGRVASTVDAATADAQRSEALFHSVLLALQADVAQQYFQVRELDAAAELYR 60 VNVSYEVDLFGRVASTVDAATADAQRSEALFHSVLLALQADVAQQYF VRELDAAA LYR Sbjct: 160 VNVSYEVDLFGRVASTVDAATADAQRSEALFHSVLLALQADVAQQYFLVRELDAAAALYR 219 Query: 61 GTVELRQESLRLVQRRFDEGDISEVDVARARSELASAQSEALGIARQRATAEHALAILLG 120 GTV+LRQESLRLVQRRFDEGDISEVDVARAR+ELASAQSEALGIARQRATAEHALAILLG Sbjct: 220 GTVDLRQESLRLVQRRFDEGDISEVDVARARAELASAQSEALGIARQRATAEHALAILLG 279 Query: 121 RTPAEFSLPPQPLTRLSVSVPAGLPSTLLERRPDIAAAERAMAAANARVGVAKSAFFPAL 180 RTPAEFSLPPQPLTRL+V+VP GLPS LLERRPDIAAAERAMAAANARVGVAKSAFFP+L Sbjct: 280 RTPAEFSLPPQPLTRLAVTVPPGLPSALLERRPDIAAAERAMAAANARVGVAKSAFFPSL 339 Query: 181 NLTGGLGYESAQLGDLFNWSSRTFLLGPLVGTALSMPLFDGGRRQAGVDRARASYEEQVA 240 NLTGGLGYESAQLGDLFNWSSRTFLLGPLVGTALSMPLFDGGRRQAGVDRARASYEEQVA Sbjct: 340 NLTGGLGYESAQLGDLFNWSSRTFLLGPLVGTALSMPLFDGGRRQAGVDRARASYEEQVA 399 Query: 241 TYRGTVLNAFREVEDNLANLRILDDQNRAQDEAVAASSRAAKLSHTQYREGAVSYFSVID 300 +YRGTVLNAFREVEDNLANLRILD QNRAQDEAVAAS+RAAKLSHTQYREGAVSYFSVID Sbjct: 400 SYRGTVLNAFREVEDNLANLRILDQQNRAQDEAVAASARAAKLSHTQYREGAVSYFSVID 459 Query: 301 ADR 303 ADR Sbjct: 460 ADR 462 >gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 507 Score = 249 bits (637), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 139/301 (46%), Positives = 202/301 (67%), Gaps = 4/301 (1%) Query: 1 VNVSYEVDLFGRVASTVDAATADAQRSEALFHSVLLALQADVAQQYFQVRELDAAAELYR 60 + ++E D++G+V V+++ A AQ S A + L+ QA VAQ Y Q+R LDA +L Sbjct: 149 LTAAWEADIWGKVRRDVESSQASAQASAADLAAARLSAQAQVAQNYLQLRVLDAQQQLLE 208 Query: 61 GTVELRQESLRLVQRRFDEGDISEVDVARARSELASAQSEALGIARQRATAEHALAILLG 120 TV+ + SL++ Q ++ G +++ DV +A+++L SAQ+ AL RA EHA+A+L+G Sbjct: 209 ETVQAYERSLQVAQNQYAAGMVAKSDVIQAQTQLKSAQASALDNGVTRAQVEHAIALLVG 268 Query: 121 RTPAEFSLPPQPLTRLSVSVPAGLPSTLLERRPDIAAAERAMAAANARVGVAKSAFFPAL 180 +TPA+F+LP PL ++ VP G+PS LLERRPD+AAAER +A+ANA++GVAK+A+FP+L Sbjct: 269 KTPAQFALPRAPLAAVAPPVPQGVPSMLLERRPDVAAAERRVASANAKIGVAKAAYFPSL 328 Query: 181 NLTGGLGYESAQLGDLFNWSSRTFLLGPLVGTALSMPLFDGGRRQAGVDRARASYEEQVA 240 L+ GY+S+ D F R + LGP L+ +FDGG R A D+A A+Y+ VA Sbjct: 329 TLSASGGYQSSTFADWFTLPGRVWSLGP----QLAQTIFDGGARAAATDQAIATYDGTVA 384 Query: 241 TYRGTVLNAFREVEDNLANLRILDDQNRAQDEAVAASSRAAKLSHTQYREGAVSYFSVID 300 TY+ TVL AF+EVEDNLA LRIL+ + Q EA+ A+ +A L + QY+ G VSY +VI Sbjct: 385 TYKQTVLTAFQEVEDNLAALRILEQEAAVQGEALQAARQAVTLVNNQYKAGTVSYVNVIT 444 Query: 301 A 301 A Sbjct: 445 A 445 >gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprotein [Herbaspirillum seropedicae SmR1] Length = 510 Score = 191 bits (484), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 137/327 (41%), Positives = 193/327 (59%), Gaps = 19/327 (5%) Query: 1 VNVSYEVDLFGRVASTVDAATADAQRSEALFHSVLLALQADVAQQYFQVRELDAAAELYR 60 +N S+E DL+GR+ +A A+AQ S A + L++QA +AQ Y Q+R D +LYR Sbjct: 159 LNASWEADLWGRIRRGAEAGEANAQASAASLAAARLSIQATLAQDYLQLRVTDLQKDLYR 218 Query: 61 GTVELRQESLRLVQRRFDEGDISEVDVARARSELASAQSEALGIARQRATAEHALAILLG 120 TV SL+L +++ G DVA+A ++L +AQ++ + + R EHA+A+L+G Sbjct: 219 RTVAAYTRSLQLSTHQYEAGTALRSDVAQAETQLRAAQAQLIDLDDTRNQLEHAIAMLIG 278 Query: 121 RTPAEFSLPPQP------------LTRLSVS---VPAGLPSTLLERRPDIAAAERAMAAA 165 + PA+FSLP P RL S +P GLPS LLERRPDIA AER AAA Sbjct: 279 KAPAQFSLPALPPAAASDSAIDANAARLQGSLPQIPVGLPSELLERRPDIANAERLAAAA 338 Query: 166 NARVGVAKSAFFPALNLTGGLGYESAQLGDLFNWSSRTFLLGPLVGTALSMPLFDGGRRQ 225 NA +GVA++A+FP L L+ GY S +LFN SR + L G+AL+ LFDGG R Sbjct: 339 NANIGVARAAYFPTLTLSASGGYNSLAFANLFNTPSRVWSL----GSALAESLFDGGARS 394 Query: 226 AGVDRARASYEEQVATYRGTVLNAFREVEDNLANLRILDDQNRAQDEAVAASSRAAKLSH 285 A D A A+Y+ VA Y+ TVL +EVED L+ LR+LD ++ Q +AV ++ A +L+ Sbjct: 395 ARNDAAVAAYDAAVAQYKQTVLGGLQEVEDKLSTLRVLDQESTVQAQAVQSAQLAERLAM 454 Query: 286 TQYREGAVSYFSVIDADRAXPPKKKPA 312 QY G V+Y SV+ A ++ A Sbjct: 455 RQYEAGTVTYLSVVTTQAASLTNQRNA 481 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 186 bits (471), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 118/303 (38%), Positives = 169/303 (55%), Gaps = 9/303 (2%) Query: 4 SYEVDLFGRVASTVDAATADAQRSEALFHSVLLALQADVAQQYFQVRELDAAAELYRGTV 63 +YE+D FGRV + DAA A +E S ++L A VAQ Y R +L T+ Sbjct: 145 AYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLVAQVAQAYLAERSYAEQLKLADETL 204 Query: 64 ELRQESLRLVQRRFDEGDISEVDVARARSELASAQSEALGIARQRATAEHALAILLGRTP 123 + RQ +L L Q+R D G + +D ++ + SA+ + RQRA AE+AL +L+G+ Sbjct: 205 KSRQSNLDLAQKRLDVGASTALDFRLTQTLVESARVAKTQLERQRAQAENALTLLVGKKI 264 Query: 124 AEFSLPPQPLT---RLSVSVPAGLPSTLLERRPDIAAAERAMAAANARVGVAKSAFFPAL 180 E LPP L ++ +PAGLPS LL RPDI +AE+++ AANA +G A++AFFP + Sbjct: 265 GE--LPPAQLLSDEKIVTDIPAGLPSDLLTNRPDIRSAEQSLLAANANIGAARAAFFPRI 322 Query: 181 NLTGGLGYESAQLGDLFNWSSRTFLLGPLVGTALSMPLFDGGRRQAGVDRARASYEEQVA 240 +LTG G S+ LG LF S + GP +LS+P+FD GR A +D A + V Sbjct: 323 SLTGNFGSASSTLGGLFESGSGAWSFGP----SLSLPIFDFGRNSANLDLAEVRKNKAVV 378 Query: 241 TYRGTVLNAFREVEDNLANLRILDDQNRAQDEAVAASSRAAKLSHTQYREGAVSYFSVID 300 TY + AFREV D L LD+Q +AQ+ +AA KL+ +++ G S +D Sbjct: 379 TYEKAIQTAFREVADALVARGSLDEQVKAQEAFLAAQQERLKLTDLRFKNGIASSLDQLD 438 Query: 301 ADR 303 A R Sbjct: 439 AQR 441 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 181 bits (459), Expect = 8e-47, Method: Compositional matrix adjust. Identities = 127/303 (41%), Positives = 183/303 (60%), Gaps = 4/303 (1%) Query: 2 NVSYEVDLFGRVASTVDAATADAQRSEALFHSVLLALQADVAQQYFQVRELDAAAELYRG 61 +VSYEVD FGRV +V+AA A A++S A + L L A++A YF +RELD ++ R Sbjct: 141 SVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAELATNYFNLRELDVELDVVRR 200 Query: 62 TVELRQESLRLVQRRFDEGDISEVDVARARSELASAQSEALGIARQRATAEHALAILLGR 121 + L++++L L R D G S +D+A+ ++ L S ++ + RQRA EHA+A L G Sbjct: 201 AIALQRQALELATARHDLGATSGLDMAQQQALLDSTLTQIDLLGRQRAQYEHAIATLTGT 260 Query: 122 TPAEFSLPPQPLTRLSVSVPAGLPSTLLERRPDIAAAERAMAAANARVGVAKSAFFPALN 181 FS+ P ++P G+PS +LERRPDIAAAERAMAAANA++GVA +A++P+ Sbjct: 261 PAPAFSITPSLTPITPPAIPVGVPSDVLERRPDIAAAERAMAAANAQIGVASAAYYPSFM 320 Query: 182 LTGGLGYESAQLGDLFNWSSRTFLLGPLVGTALSMPLFDGGRRQAGVDRARASYEEQVAT 241 L G +S LFN S + L G + S LFDGGR +AGVD ++A YE VA+ Sbjct: 321 LQPSYGVDSRNWATLFNAPSLLWSL----GVSASQSLFDGGRLRAGVDFSQAGYEATVAS 376 Query: 242 YRGTVLNAFREVEDNLANLRILDDQNRAQDEAVAASSRAAKLSHTQYREGAVSYFSVIDA 301 YR TVL A +EVED + L L+ A+A++ R +++ ++Y GA Y VI A Sbjct: 377 YRRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVLEIASSRYEGGATPYLDVITA 436 Query: 302 DRA 304 ++ Sbjct: 437 QQS 439 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.131 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 915,605 Number of extensions: 32984 Number of successful extensions: 223 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 188 Number of HSP's successfully gapped: 18 Length of query: 354 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 268 Effective length of database: 1,212,660 Effective search space: 324992880 Effective search space used: 324992880 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)