BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0148.p (555 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309652|ref|YP_003773744.1| HD-GYP domain-containing protei... 978 0.0 gi|300309839|ref|YP_003773931.1| metal-dependent phosphohydrolas... 159 4e-40 gi|300310565|ref|YP_003774657.1| response regulator containing a... 75 2e-14 gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent p... 69 8e-13 gi|300313980|ref|YP_003778072.1| two component response regulato... 69 9e-13 >gi|300309652|ref|YP_003773744.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 552 Score = 978 bits (2528), Expect = 0.0, Method: Compositional matrix adjust. Identities = 473/545 (86%), Positives = 507/545 (93%), Gaps = 1/545 (0%) Query: 12 PTTGEHRDIAYRLERLTELCRELGSQRDIPLLLERILLTARDITRADGGTLYRTTDHGDA 71 P HR+IAYRLERLTELCRELGS RDIPLLLERILLTA+DIT+ADGGTLYR + GD+ Sbjct: 8 PAAERHRNIAYRLERLTELCRELGSSRDIPLLLERILLTAKDITQADGGTLYRINEGGDS 67 Query: 72 LRFDISVNQTLKMHQGGSSGRAVSIPDIALTLPDGQPNLAAVAAYAANTRESVNIPDVYQ 131 LRF ISVN TLKMHQGGSSG+A+SIPDIAL LPDGQPNL+AVAAYAANTR+SVNI DVYQ Sbjct: 68 LRFYISVNDTLKMHQGGSSGKAISIPDIALRLPDGQPNLSAVAAYAANTRKSVNIADVYQ 127 Query: 132 -ASGFNFNGMRLFDEKYGYRSKSILTVPMQDHEGELVGVLQLINAIDPDSGVPVPFSETD 190 +SGFNFNGMRLFDEKYGY+SKS LTVPM+DHEGELVGVLQLINAIDP +G P+PFSETD Sbjct: 128 VSSGFNFNGMRLFDEKYGYQSKSFLTVPMEDHEGELVGVLQLINAIDPATGAPLPFSETD 187 Query: 191 QRFIEALASQAAMAMTNQQLIARLENLFESLIKLINIGIGEKSPHTGRHCEQVPQLAMML 250 Q FIEALASQAAMAMTNQQLI RLE LFESLI+LINIGIGEKSPHTGRHCEQVP+LAMML Sbjct: 188 QHFIEALASQAAMAMTNQQLIQRLEQLFESLIRLINIGIGEKSPHTGRHCEQVPELAMML 247 Query: 251 AEAAHNTTEGPLADFRMTDADRRELWLAALLHDCGKIGTPSHIVEKATKLETIFDRIHLI 310 AEAAHN TEGPLADF M++ADRRELWLAALLHDCGKI TP+HIVEKATKLETIFDRIHL+ Sbjct: 248 AEAAHNATEGPLADFHMSEADRRELWLAALLHDCGKISTPTHIVEKATKLETIFDRIHLV 307 Query: 311 DTRFEVLKRDAQIRVLQEKLALGAALTPERSAELDAGLQAEFLQMEDERAFLRRINYGNE 370 +TR EVLKRDA+IRVLQEKLALGAAL +R+AELDAGL AE Q++ ERAFLR+IN G E Sbjct: 308 ETRIEVLKRDAEIRVLQEKLALGAALDAQRAAELDAGLAAELAQLDLERAFLRKINVGTE 367 Query: 371 GMRAEDQQRVLDIAERSWRGPDGQIRKLLDEEEAGNLRIRAGTLNDAERAIINNHIVITI 430 GMR EDQQRVLDIA+RSWRGPDGQ+R LLDEEE+ NLRIRAGTLNDAER IINNHIVITI Sbjct: 368 GMRPEDQQRVLDIAQRSWRGPDGQLRPLLDEEESANLRIRAGTLNDAERQIINNHIVITI 427 Query: 431 KMLESLPWPKQLARVTEFAGGHHERMDGKGYPKGLTREQMSVQARIMAVADIFEAVTAAD 490 KMLESLPWPKQLARVTEFAGGHHERMDGKGYPKGLTREQMSVQARIMAVADIFEAVTAAD Sbjct: 428 KMLESLPWPKQLARVTEFAGGHHERMDGKGYPKGLTREQMSVQARIMAVADIFEAVTAAD 487 Query: 491 RPYKRGNTLNEAVDILAGFKARNHIDPDIFDLFVRERIYEKYAEQFMHPSQIDPVDLSRI 550 RPYKRGNTLNEAV+ILAGFKAR+HIDPD+FDLFVRERIYEKYAE+FMHPSQID VDLSRI Sbjct: 488 RPYKRGNTLNEAVEILAGFKARDHIDPDLFDLFVRERIYEKYAEKFMHPSQIDEVDLSRI 547 Query: 551 PGHGG 555 PG G Sbjct: 548 PGFGA 552 >gi|300309839|ref|YP_003773931.1| metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 975 Score = 159 bits (403), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 94/230 (40%), Positives = 138/230 (60%), Gaps = 12/230 (5%) Query: 159 MQDHEGELVGVLQLINAIDPDSGVPVPFSETDQRFIEALASQAAMAMTNQQLIARLENLF 218 ++ G L G+L L++A D G P + F L+ A+A+ +QLIA + L Sbjct: 523 LRGRNGSLEGLLVLVHAHDSAHGDPRFLA-----FARRLSGMLAVAIETRQLIAAQKALL 577 Query: 219 ESLIKLINIGIGEKSPHTGRHCEQVPQLAMMLAEAAHNTTEGPLADFRMTDADRRELWLA 278 ESLI+L+ I KSP+TG HCE+VP LA+ML E EGP A F + + +R LA Sbjct: 578 ESLIRLMADAIDAKSPYTGGHCERVPHLAIMLVERLAQEREGPYAGFSLNEDERYAFRLA 637 Query: 279 ALLHDCGKIGTPSHIVEKATKLETIFDRIHLIDTRFEVLKRDAQIRVLQEKLALGAALTP 338 ALLHDCGK+ +P HIV+KATKLE I +RIH + RFEVL RDA++ +L + L AL+ Sbjct: 638 ALLHDCGKVTSPEHIVDKATKLEVIHNRIHEVRMRFEVLWRDAELALLHQ---LPQALSE 694 Query: 339 ERSAELDAGLQAEFLQMEDERAFLRRINYGNEGMRAEDQQRVLDIAERSW 388 +++ L+A+ ++ ++ AF+ + N G+E M E R+ IA ++W Sbjct: 695 QQA----QALEAQRARLREDFAFVAQCNVGSEAMSEEAIARLARIATQTW 740 Score = 155 bits (393), Expect = 6e-39, Method: Compositional matrix adjust. Identities = 74/153 (48%), Positives = 101/153 (66%) Query: 403 EAGNLRIRAGTLNDAERAIINNHIVITIKMLESLPWPKQLARVTEFAGGHHERMDGKGYP 462 E NL IR GTL +R INNHIV T+ ML+SLPWP+ L V + A HHE+MDG GYP Sbjct: 819 ELHNLGIRRGTLTPEDRFAINNHIVQTLVMLKSLPWPESLKSVPDTAANHHEKMDGTGYP 878 Query: 463 KGLTREQMSVQARIMAVADIFEAVTAADRPYKRGNTLNEAVDILAGFKARNHIDPDIFDL 522 + L ++ +Q R+MA+AD+FEA+TAADRPYK TL+E++ I+A H+D ++F Sbjct: 879 RRLKASELPLQDRVMALADVFEALTAADRPYKPAKTLSESLRIMANMCRERHLDTELFRY 938 Query: 523 FVRERIYEKYAEQFMHPSQIDPVDLSRIPGHGG 555 F+R R++E YA + + QID VD+ I G Sbjct: 939 FLRSRLWEVYAGELLKEGQIDAVDIVEIEALTG 971 >gi|300310565|ref|YP_003774657.1| response regulator containing a CheY-like receiver domain and an HD-GYP domain [Herbaspirillum seropedicae SmR1] Length = 348 Score = 74.7 bits (182), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 46/134 (34%), Positives = 73/134 (54%), Gaps = 8/134 (5%) Query: 406 NLRIRAGTLNDAERAIINNHIVITIKMLESLPWPKQLARVTEFAGGHHERMDGKGYPKGL 465 ++ ++ G L++ E ++ H I +L + P P L ++ A HHE+ DG GYP GL Sbjct: 216 HILLKPGRLDEEELKVMRMHARIGADILVASPSPL-LQTASQIALTHHEKWDGSGYPNGL 274 Query: 466 TREQMSVQARIMAVADIFEAVTAADRPYKRGNTLNEAVDILAGFKARNHIDPDIFDLFVR 525 EQ+ + RI+AVAD+F+A+T++ RPYK G +L A L ++ H DP + F Sbjct: 275 KGEQIPLYGRIVAVADVFDALTSS-RPYKPGWSLERASQYLRE-QSGAHFDPRCVEAFFA 332 Query: 526 E-----RIYEKYAE 534 I+ +Y E Sbjct: 333 GWEEVLEIFNRYQE 346 >gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 406 Score = 69.3 bits (168), Expect = 8e-13, Method: Compositional matrix adjust. Identities = 40/116 (34%), Positives = 64/116 (55%), Gaps = 5/116 (4%) Query: 410 RAGTLNDAERAIINNHIVITIKMLESLPWPKQLARVTEFAGGHHERMDGKGYPKGLTREQ 469 + G L+D E + I H + K L P AR + HHE++DG GYP+GL Sbjct: 208 KPGKLSDEEFSQIKKHPELGYKYLSQDPNVPDYAR--DVCLHHHEKIDGNGYPEGLRAGA 265 Query: 470 MSVQARIMAVADIFEAVTAADRPYKRGNTLNEAVDILAGFKARNHIDPDIFDLFVR 525 +++ +R+ A+ D+++A+T +DRPYKR EA+ +A + H D +F FV+ Sbjct: 266 ITLYSRMAAICDVYDALT-SDRPYKRAWDPAEAISAMASWT--GHFDRAMFLAFVK 318 >gi|300313980|ref|YP_003778072.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 199 Score = 68.9 bits (167), Expect = 9e-13, Method: Compositional matrix adjust. Identities = 33/98 (33%), Positives = 61/98 (62%), Gaps = 4/98 (4%) Query: 407 LRIRAGTLNDAERAIINNHIVITIKMLESLPWPKQLARVTEFAGGHHERMDGKGYPKGLT 466 L + G ++ E ++ H+ ++L + +P LA E HHE +DG GYP+G++ Sbjct: 74 LLTKPGRISVEEYDVLKTHVQFGYEILNKISFPFNLA---EIVHCHHEYLDGSGYPRGIS 130 Query: 467 REQMSVQARIMAVADIFEAVTAADRPYKRGNTLNEAVD 504 +++ ++ARI+ VADI E+++ ADRPY++ ++ A+D Sbjct: 131 GDEIPLEARILTVADIVESMS-ADRPYRKSLGMSAALD 167 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,675,723 Number of extensions: 68671 Number of successful extensions: 220 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 216 Number of HSP's successfully gapped: 10 Length of query: 555 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 465 Effective length of database: 1,193,720 Effective search space: 555079800 Effective search space used: 555079800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)