BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0022.0142.p
         (493 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309648|ref|YP_003773740.1| hemolysin activation/secretion ...   784   0.0  
gi|300312549|ref|YP_003776641.1| hemolysin activation/secretion ...    63   7e-11

>gi|300309648|ref|YP_003773740.1| hemolysin activation/secretion
           protein [Herbaspirillum seropedicae SmR1]
          Length = 569

 Score =  784 bits (2024), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 376/468 (80%), Positives = 419/468 (89%)

Query: 12  ERFDITRFAVEGNTLLTQARVDEAVTPFRGPGRVYGDIQQALEALENAYRSAGYTAVQVS 71
           ERF+I RF V GNTLL Q +V  AV PF G GRVYGDIQQALEALEN YRSAGYTAVQV 
Sbjct: 51  ERFEIRRFVVAGNTLLPQDQVQAAVAPFSGQGRVYGDIQQALEALENLYRSAGYTAVQVH 110

Query: 72  VPEQELTGGVVRLQVVETVVGKVVVSDNRHFSEQNIRASIPQLVEGSAPNLRRISESVQL 131
           VPEQEL+ G VR+ VVETVVGKV VS+NRHFS+ NIRASIP L EG  PNLRRISESVQL
Sbjct: 111 VPEQELSSGEVRIDVVETVVGKVQVSENRHFSQANIRASIPALKEGVPPNLRRISESVQL 170

Query: 132 ANDNPAKQVNVTLTASESTNQIDAAITVKDDNPLRVMLNVDNSGNRDTGQWRTGVAVQHS 191
           ANDNPAKQVNVTL+A++S +QIDA I VKDD+PLRVMLN+DN+G+ DTG+WRTGVA+QHS
Sbjct: 171 ANDNPAKQVNVTLSAAQSGDQIDAKIEVKDDDPLRVMLNLDNTGSADTGRWRTGVAIQHS 230

Query: 192 NLFNRDHVATVAYTTSPDSPSGAKVDLYSLGYRLPMYGLGDSLDFIYGKSNVTSGQTLAV 251
           NLFNRDHVATVAYTTSPDSPSG+KVDLYSLGYRLP+Y LGDSLDFIYGKS+V+SGQTLA+
Sbjct: 231 NLFNRDHVATVAYTTSPDSPSGSKVDLYSLGYRLPLYELGDSLDFIYGKSSVSSGQTLAL 290

Query: 252 NSTLNITGRGEIYALRWNHYLARSGEWSSKLVFGADYKKVDSSCNINGGLLSGASLNTCL 311
           NSTLNITGRGE+YALRWNH+ AR+GEWSSKLV GADYKKVDSSC INGGLL+GA+LNTCL
Sbjct: 291 NSTLNITGRGEVYALRWNHFFARNGEWSSKLVLGADYKKVDSSCAINGGLLNGATLNTCL 350

Query: 312 PYSTLPLSVSYSAQQQGVGQVLDYNLGIARNMATGEARRNDALQVTDRYSLFTGSRQSMD 371
           PYSTLPLS++YSAQ+QGVG+V+D N+G+ARNMATG+ARRNDALQVTDRYSLFT  RQSMD
Sbjct: 351 PYSTLPLSLTYSAQRQGVGEVIDANIGVARNMATGQARRNDALQVTDRYSLFTNGRQSMD 410

Query: 372 NFIIVRGGASWFKVFQNDWQVRLASSLQVSPHALPPVEQFGLAGANTVRGFTERAVAADS 431
           NFI VRGGASW K FQNDWQ+RLA +LQ+SPHALP VEQFGLAGA  VRGFTERAV+ADS
Sbjct: 411 NFIAVRGGASWLKAFQNDWQMRLAGNLQLSPHALPAVEQFGLAGATAVRGFTERAVSADS 470

Query: 432 GAVVNAELYTPDLLVKSEARGNLRLLGFFDAARGANNNVTALSTTPST 479
           G V+N+ELYTPDLL KSE +G++RLL F DA RGANNNVTA S   ST
Sbjct: 471 GVVLNSELYTPDLLAKSELKGSMRLLAFVDAGRGANNNVTANSQVQST 518


>gi|300312549|ref|YP_003776641.1| hemolysin activation/secretion
           protein [Herbaspirillum seropedicae SmR1]
          Length = 584

 Score = 62.8 bits (151), Expect = 7e-11,   Method: Compositional matrix adjust.
 Identities = 106/460 (23%), Positives = 178/460 (38%), Gaps = 52/460 (11%)

Query: 2   AAGSAPVRNDERFDITRFAVEGNTLLTQARVDEAV-TPFRGPG-RVYG--DIQQALEALE 57
           AA + P +    F + R  ++G      AR D  +  P   PG R  G   I   + AL+
Sbjct: 77  AAEAVPAQESPCFAVHRVVLDGPG---SARFDWLLDLPEANPGGRCLGVQGINAVMSALQ 133

Query: 58  NAYRSAGYTAVQVSVPEQELTGGVVRLQVVETVVGKVVVSDNR--HFSEQNIRASIPQLV 115
           NA  + GY   ++    Q++  G +RL +V   +  +  +DN     ++ N   + P   
Sbjct: 134 NAIVARGYVTTRILAQPQDIASGELRLTIVPGKIRAIRFADNADPRATQWNALPASP--- 190

Query: 116 EGSAPNLRRISESVQLANDNPAKQVNVTLTASESTNQI----DAAITVKDDNPLRVMLNV 171
            G   NLR I + ++     P  + +V +  SE  +      D  I+ +   P+R+ L+V
Sbjct: 191 -GDLLNLRDIEQGLENLKRVPTAEADVQIAPSEGKDAAPGDSDLVISYQQGIPIRLTLSV 249

Query: 172 DNSGNRDTGQWRTGVAVQHSNLFNRDHVATVAYT--TSPDSPSGAKVDLYSLGYRLPMYG 229
           D+SG + TG+++    V   N    + +  ++++       P         + Y LP   
Sbjct: 250 DDSGTKATGKYQGSATVSLDNWLTLNDLFYISFSHDLGGGDPGARGSQSQVVHYSLP--- 306

Query: 230 LGDSLDFIYGKSNVTSGQTLAVNSTLNITGRGEIYALRWNHYLARSGEWSSKLVFGA--- 286
           LG         SN        +N T   +G+ E   L+ +  + R     + L F     
Sbjct: 307 LGYWALAFTNSSNRYHQAVAGINQTYLYSGQSETNELKLSRLVYRDAVRKTTLSFKGFVR 366

Query: 287 ------DYKKVDSSCNINGGLLSGASLNTCLPYSTLPLSVSYSAQQQGVGQVLDYNLGIA 340
                 D  +V        G   G +    +   TL L+++Y   ++G G    ++   A
Sbjct: 367 SSSNYVDDTEVQVQRRRVAGWEMGLAHREFIGEGTLDLALAY---KRGTGA---FDAMAA 420

Query: 341 RNMATGEARRNDALQVTDRYSLFTGSRQSMDNFIIVRGGASWFKVFQNDWQVRLASSLQV 400
              A GE         T R+ L T        F +      W K      +  L +  Q 
Sbjct: 421 PEEAFGEG--------TSRFRLMTADLNLNAPFALP---MPWGK---QAMRYTLGARAQW 466

Query: 401 SPHALPPVEQFGLAGANTVRGFT-ERAVAADSGAVVNAEL 439
           +   L P ++F L G  +VRGF  E  + A+ G  V  EL
Sbjct: 467 NRTPLTPQDKFSLGGRYSVRGFDGESNLLAERGWYVRNEL 506


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.315    0.131    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,491,141
Number of extensions: 61923
Number of successful extensions: 220
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 219
Number of HSP's successfully gapped: 3
Length of query: 493
Length of database: 1,619,870
Length adjustment: 89
Effective length of query: 404
Effective length of database: 1,198,455
Effective search space: 484175820
Effective search space used: 484175820
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 132 (55.5 bits)