BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0139.p (280 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspiri... 447 e-127 gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 108 6e-25 gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbasp... 98 9e-22 gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Her... 96 3e-21 gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 87 2e-18 >gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspirillum seropedicae SmR1] Length = 280 Score = 447 bits (1151), Expect = e-127, Method: Compositional matrix adjust. Identities = 216/280 (77%), Positives = 244/280 (87%) Query: 1 MQPIPMIPLRNGAQVPALGQGTWNMGESAAAASAEVRALQAGIDLGMTLIDTAEMYADGQ 60 MQ IP++ LRNG VP LGQGTWNMGES A A+AEVRALQAG+DLGM+LIDTAEMYA+G Sbjct: 1 MQSIPLLSLRNGQSVPILGQGTWNMGESPAQANAEVRALQAGLDLGMSLIDTAEMYAEGG 60 Query: 61 SERVVGKAIAGRREQVYLVSKVLPHNASERGTIAACEASLKRLGTDHLDLYLLHWRGSHP 120 +ER+V KAIAGRR+QVYLVSKVLPHNAS+RGTIAACEASLKRLGTD +DLYLLHWRG+HP Sbjct: 61 AERIVAKAIAGRRDQVYLVSKVLPHNASKRGTIAACEASLKRLGTDRIDLYLLHWRGAHP 120 Query: 121 LSATIAGMETLIAQGKIRGWGVSNLDTDDMDELADAPGGEQVLCNQVLYNLSRRGIEYDL 180 L+ATI GMETL++QGKI GWGVSN DTDD+D+L + P GE LCNQVLYNLSRRGIEYDL Sbjct: 121 LAATIEGMETLVSQGKIGGWGVSNFDTDDIDDLLEEPHGEHYLCNQVLYNLSRRGIEYDL 180 Query: 181 LPQAEQSGVAVMAYSPIEQGRILKQATLKAVAQRHGATPAQVALAWVLRRPGVIAIPKAA 240 LPQA+Q+ +AVMAYSPIEQGRILK L A+AQ H TPAQ+ALAWVLR+PGV+AIPKAA Sbjct: 181 LPQAQQAHIAVMAYSPIEQGRILKHPALTALAQAHRVTPAQIALAWVLRQPGVVAIPKAA 240 Query: 241 DVAHVEANRACLALHLNENDYSELDAAFPPPRRKQPLAML 280 DVAHV+ANRA L + L+ D LD AF PPRRKQPLAML Sbjct: 241 DVAHVQANRASLDISLSAADLKILDEAFAPPRRKQPLAML 280 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 108 bits (269), Expect = 6e-25, Method: Compositional matrix adjust. Identities = 89/305 (29%), Positives = 140/305 (45%), Gaps = 49/305 (16%) Query: 11 NGAQVPALGQGTWNMGESAAAA--SAEVRALQAGIDLGMTLIDTAEMYADGQSERVVGKA 68 NG V A+G G M + A + + + ++LG+ L+DTA+MY +E ++G+A Sbjct: 23 NGPLVSAIGLGCMGMSDFYAQRDDAESLATIDRALELGINLLDTADMYGPYTNEELLGRA 82 Query: 69 IAGRREQVYLVSKV----LPHNASERGTIA-------ACEASLKRLGTDHLDLYLLH-WR 116 I GRR++ ++ +K P + RG A E SLKRLG + +DLY H Sbjct: 83 IKGRRDKFFIATKFGIRRDPSDPGARGVDGSPAYIRQAVEGSLKRLGVETIDLYYQHRID 142 Query: 117 GSHPLSATIAGMETLIAQGKIRGWGVSNLDTDDMDELADAPGGEQVLCNQVLYNLSRRGI 176 + P+ T+ + L+ GKIR G+S ++ A V Q Y+L R Sbjct: 143 PATPIEVTMGVLADLVQAGKIRYIGLSEASAATLER---AHRVHPVTALQSEYSLWTRDP 199 Query: 177 EYDLLPQAEQSGVAVMAYSPIEQG------------------------------RILKQA 206 E ++L G+ +AYSP+ +G R L+ A Sbjct: 200 EAEVLAACRTLGIGFVAYSPLGRGFLTGAIQRFEDLAEDDFRRSNPRFQGENFARNLQLA 259 Query: 207 -TLKAVAQRHGATPAQVALAWVL-RRPGVIAIPKAADVAHVEANRACLALHLNENDYSEL 264 +K +A +HG TP+Q+ALAWV+ + P ++ IP ++E N + + L D L Sbjct: 260 EKVKEMAGQHGCTPSQLALAWVMAQDPHIVPIPGTKRRRYLEDNAGSVGVKLAPEDLQAL 319 Query: 265 DAAFP 269 DA FP Sbjct: 320 DAIFP 324 >gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 349 Score = 97.8 bits (242), Expect = 9e-22, Method: Compositional matrix adjust. Identities = 93/336 (27%), Positives = 143/336 (42%), Gaps = 83/336 (24%) Query: 17 ALGQGTWNMGESAAAASAEVRALQAGIDLGMTLIDTAEMY-------ADGQSERVVGKAI 69 LG TW S A A A+ L ++ G+ LID AEMY G +ER +G + Sbjct: 17 TLGTMTWGEQNSEADAHAQ---LDYAVERGINLIDVAEMYPVPPKAETQGLTERYLGTWL 73 Query: 70 --AGRREQVYLVSKVL-----PHNA----------SERGTIAACEASLKRLGTDHLDLYL 112 +G+R+Q+ + +K PHN +G A SL+RL DH+DLY Sbjct: 74 KKSGKRDQLLIATKCTGPARKPHNPRHVRGGINNLDRKGLTEALHGSLERLQLDHVDLYQ 133 Query: 113 LHW------------------RGSHPLSATIAGMETLIAQGKIRGWGVSNLDTDDMDEL- 153 LHW + P+ T+ + + GKIR G+SN + + Sbjct: 134 LHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVKAGKIRTIGLSNETAWGVAQFL 193 Query: 154 --ADAPGGEQVLCNQVLYNLSRRGIEYDLLPQAEQSGVAVMAYSPIEQGRILKQ------ 205 A+ G E+++ Q YNL R E +L A + V ++AYSP+ G + + Sbjct: 194 RAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVGLLAYSPLAFGMLSGKYLDGAR 253 Query: 206 ------------------------ATLKAVAQRHGATPAQVALAWVLRRPGVIA-IPKAA 240 A A+A++HG PAQ+ALA+V RP + + I A Sbjct: 254 PAGGRLTLFERFSRYTNAQAERATAEYVALARQHGLDPAQMALAYVNSRPFLTSNIIGAT 313 Query: 241 DVAHVEANRACLALHLNENDYSELDAAFPPPRRKQP 276 +A + +N L L E+ + ++A R+QP Sbjct: 314 TLAQLRSNIDSAELQLGEDVLAAIEAI----HRRQP 345 >gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 329 Score = 96.3 bits (238), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 88/298 (29%), Positives = 140/298 (46%), Gaps = 56/298 (18%) Query: 10 RNGAQVPALGQGTWNMGESAAAASAE--VRALQAGIDLGMTLIDTAEMYADGQSERVVGK 67 R GAQV +G G W +G S SA+ AL A +D G++ IDTA++Y DG+SE+++ + Sbjct: 8 RTGAQVSEIGFGAWAIGGSWGDVSAQDAKAALHAALDAGVSFIDTADVYGDGRSEQLIAQ 67 Query: 68 AIAGR-REQVYLVSK----VLPHNAS---ERGTIAA-CEASLKRLGTDHLDLYLLHWRGS 118 + R E+ ++ +K + PH AS +R ++A + SLK LG D LDL LH + Sbjct: 68 VLKERGGERPFVATKAGRRLSPHVASGYNDRAQLSAFIDRSLKNLGVDCLDLVQLHCPPT 127 Query: 119 --HPLSATIAGMETLIAQGKIRGWGVSNLDTDDMDELADAPGGEQVLCNQVLYNLSRRGI 176 + ++ L+A GKIR +GVS ++ + + P + V Q+++N+ R+ Sbjct: 128 EVYYQQEVFGHLDALVAAGKIRHYGVSVEKVEEALKAIEYPNVKSV---QIIFNIFRQRP 184 Query: 177 EYDLLPQAEQSGVAVMAYSPIEQ----GRILKQATLKAVAQR------------------ 214 L +A++ +AV+A P+ G++ Q T A R Sbjct: 185 ISLFLQEAQRRDIAVIARVPLASGLLTGKMSAQTTFAADDHRQFNRHGEAFDVGETFSGV 244 Query: 215 -----------------HGATPAQVALAWVLRRPGV-IAIPKAADVAHVEANRACLAL 254 G AQ AL W+L G+ IP A +VA ++N A AL Sbjct: 245 PYEVALAAVEKIRALVPQGVPMAQFALRWILMESGISTVIPGARNVAQAQSNSAASAL 302 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 87.0 bits (214), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 66/203 (32%), Positives = 99/203 (48%), Gaps = 13/203 (6%) Query: 10 RNGAQVPALGQGTWNMGESAAAASAEVRALQAGIDLGMTLIDTAEMYADGQSERVVGKAI 69 ++ +V AL G+ G+ + R + + + G+ IDTA++Y G+SE +VGK + Sbjct: 8 KSNLKVSALCLGSMMFGDQTDLQESG-RIVASAREHGVNFIDTADVYTKGKSEEIVGKLL 66 Query: 70 AGRREQVYLVSKV------LPH--NASERGTIAACEASLKRLGTDHLDLYLLHW-RGSHP 120 AG+R + L SK+ P+ N S + E L RL TD+LD+ +H Sbjct: 67 AGQRHEWVLASKLGNAMSGKPNESNYSRSWIVRETENILTRLDTDYLDILYMHRDYQDEN 126 Query: 121 LSATIAGMETLIAQGKIRGWGVSNLDTDDMDELADAPGGEQV---LCNQVLYNLSRRGIE 177 L + + LI GKIRG+GVSN + E+ V L Q YNL R E Sbjct: 127 LEEAVQALGDLIRVGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPLVCQPYYNLLNRQPE 186 Query: 178 YDLLPQAEQSGVAVMAYSPIEQG 200 ++LP G+ V+ YSPI +G Sbjct: 187 VEILPACAHYGLGVVPYSPIARG 209 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 920,137 Number of extensions: 37954 Number of successful extensions: 178 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 164 Number of HSP's successfully gapped: 6 Length of query: 280 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 196 Effective length of database: 1,222,130 Effective search space: 239537480 Effective search space used: 239537480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)