BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0137.p (482 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312616|ref|YP_003776708.1| major facilitator superfamily p... 130 3e-31 gi|300310481|ref|YP_003774573.1| major facilitator superfamily p... 122 8e-29 gi|300310666|ref|YP_003774758.1| major facilitator superfamily p... 115 9e-27 gi|300310615|ref|YP_003774707.1| major facilitator superfamily p... 110 2e-25 gi|300313879|ref|YP_003777971.1| major facilitator superfamily p... 110 4e-25 >gi|300312616|ref|YP_003776708.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 414 Score = 130 bits (326), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 121/439 (27%), Positives = 196/439 (44%), Gaps = 40/439 (9%) Query: 30 TRSHHTIIFLILIGCLFDAFEQNAIGLVGPLLKQQWGLTATDIGLLNTVTFASAAIGRIV 89 T S + G D F+ + P L W ++ + G + T ++AIG Sbjct: 7 TTSERRTLTATFTGYAVDGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTSAIGGWA 66 Query: 90 SGLVADRYGRRVMLSLDLLLFTLGAGICALAPDLTVMAVGRAIVGFGLGGEIAIAVTMLA 149 +G++ADRYGR +L +L F + + RA+ GFG+GGE A+ ++A Sbjct: 67 AGVLADRYGRVRVLQWTVLWFAFFTFLSGFTTSFGQLFFTRAMQGFGMGGEWAVGSVLIA 126 Query: 150 EFCSSKFRGTAVGLV--NVGAGGMGNFLAPAFGLLVFTLFPGENSWRWLFFCLMLPALLG 207 E S++ RG A GLV + G G+ LA +G V+ + P E +W+ LF+ +LPALL Sbjct: 127 ETISARHRGKASGLVQSSWAVGWAGSALA-FWG--VYAVLPPETAWKVLFWIGVLPALLT 183 Query: 208 AFYRRYIPETPRFLLSRNKIAEANQVLARLASGKLSGPAPQGRDWISDDGSRQQTAPAKV 267 + RR I E +L ++ ++ E+ Q + Sbjct: 184 LYIRRAISEPAVYLATQARLRESGQ---------------------------------RG 210 Query: 268 RSMEIFQGALARRTLPLCIAIWMTYGAQISVLTLMPTILVSLGY-TISKSLLFTMVMQSG 326 + IF L R TL + GA SV T +PT L + + ++ + + +V+ G Sbjct: 211 NFLRIFHRDLLRTTLLASLLATGMQGAYYSVTTWLPTFLKTERHLSVLGTSTYLLVLIGG 270 Query: 327 SLLGAIAASVFGFHLPRKRVLTAGAICACLAALAIGFLAKSIVLILAAGAVFQFFVLLLN 386 S G + ++ + R+R A + L+ + + +LA G V FF+ + Sbjct: 271 SFCGYLTSAWLSDTIGRRRCFMLFAAMGIVLVLSYTQMIITDAWMLALGFVLGFFLSGIF 330 Query: 387 TTIWIFAPELYPTRVRAFGTAFILATGTAAGALMPLVAGRLFDAFGLAGVFGMIAA-MYG 445 + + + EL+P+ VR G F G A GA+ P + G L ++ L G IAA YG Sbjct: 331 SGMGAYLSELFPSDVRGSGQGFCYNFGRAVGAICPALVGYLSNSLPLGQTIGWIAAGCYG 390 Query: 446 IFTVAIRTVPETYGKSPDA 464 + VA +PET GK +A Sbjct: 391 LVIVACLVLPETRGKELEA 409 >gi|300310481|ref|YP_003774573.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 460 Score = 122 bits (305), Expect = 8e-29, Method: Compositional matrix adjust. Identities = 131/477 (27%), Positives = 209/477 (43%), Gaps = 43/477 (9%) Query: 13 HSPRVAELDGALDKIGVTRSHHTIIFLILIGCLFDAFEQNAIGLVGPLLKQQWGLTATDI 72 H + ++ LD + ++ L + + D F+ IG P L ++WG+T Sbjct: 7 HGNQTVDIGRMLDDGVFSGFQKLVVCLAALSIVMDGFDGQLIGFAIPSLIKEWGITRNAF 66 Query: 73 GLLNTVTFASAAIGRIVSGLVADRYGRRVMLSLDLLLFTLGAGICALAPDLTVMAVGRAI 132 IG +GL ADR+GRR+ + + +F + + A +PD+T +A+ R Sbjct: 67 APAVAAGLVGMGIGSAFAGLFADRFGRRMAIIGSVFVFGVATSLIAFSPDVTTIAILRFC 126 Query: 133 VGFGLGGEIAIAVTMLAEFCSSKFRGTAV--GLVNVGAGGMGNFLAPAFGLLVFTLFPGE 190 G G+GG + + TM AEF ++ R AV +V V GGM LA GL + P Sbjct: 127 AGLGIGGALPSSTTMTAEFSPARRRTLAVTATIVCVPLGGM---LA---GLFANAVLP-H 179 Query: 191 NSWRWLFFC-LMLPALLGAFYRRYIPETPRFLLSR-NKIAEANQVLARLASGKLSGPAPQ 248 WR LF +LP IPE+PR+L ++ E ++L R++ P Sbjct: 180 YGWRGLFLIGGLLPVAFSVLLWFVIPESPRYLARHPHRWDELRRLLQRMSR-------PV 232 Query: 249 GRDWISDDGSRQQTAPAKVRSMEIFQGALARRTLPLCIAIWMTYGAQISVLTLMPTILVS 308 D D +R+Q + +F AR T+ L +A +M A S + +PT+L Sbjct: 233 SADTGFAD-AREQAIETRAGFGALFASGQARDTMALWVAFFMNLLAVYSAFSWLPTMLAG 291 Query: 309 LGYTISKSLLFTMVMQSGSLLGAIAASV----FGFHLPRKRVLTAGAICACLAALAIGFL 364 G + S + G + GA+ ++ FG LP + CLAA A Sbjct: 292 EGLSPSVAGAGLTAYNFGGVFGALLCALFITRFGSRLPM--------LLCCLAATASALF 343 Query: 365 AKSI------VLILAAGAVFQFFVLLLNTTIWIFAPELYPTRVRAFGTAFILATGTAAGA 418 +S+ L++ + FV + +T++ +YPT+VRA GTA LA G GA Sbjct: 344 LRSLDVASQTTLLVVGIGLHGLFVNAVQSTMYALCAYIYPTQVRATGTAAALAFGR-LGA 402 Query: 419 LMPLVAGRL-FDAFGLAGVFGMIA-AMYGIFT---VAIRTVPETYGKSPDAPLEETE 470 ++ AG L G AG M+A AM +F V + +P + + +E+ Sbjct: 403 ILSAFAGALVITQGGAAGYLAMLAGAMLMVFVALLVVRKHIPRVNAGAAETLKKESH 459 >gi|300310666|ref|YP_003774758.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 438 Score = 115 bits (287), Expect = 9e-27, Method: Compositional matrix adjust. Identities = 107/394 (27%), Positives = 174/394 (44%), Gaps = 25/394 (6%) Query: 18 AELDGALDKIGVTRSHHTIIFLILIGCLFDAFEQNAIGLVGPLLKQQWGLTATDIGLLNT 77 A ++ L GV + + + + + L DA + +IG P + + +GLT T Sbjct: 6 ASVEQGLQAAGVGKFQYRLFVIFGLVWLADAMQVLSIGFSAPTIAKTFGLTVPQALQTGT 65 Query: 78 VTFASAAIGRIVSGLVADRYGRRVMLSLDLLLFTLGAGICALAPDLTVMAVGRAIVGFGL 137 + F +G V G +ADR GRR +L +++ A APD T + R + G G+ Sbjct: 66 MFFVGMLLGAFVFGRLADRIGRRPVLMGAVVIDACFGVASAFAPDFTWLLALRLLTGIGV 125 Query: 138 GGEIAIAVTMLAEFCSSKFRGTAVGLVNVGAGGMGNFLAPAFGLLVFTLFPGENSWRWLF 197 GG + + TM+AEF S RG + ++ +G L+ + G ++WR +F Sbjct: 126 GGTLPVDYTMMAEFLPSARRGRWLVMLE-SFWAIGTICLAVLALVAVSW--GNDAWRVIF 182 Query: 198 FCLMLPALLGAFYRRYIPETPRFLLSRNKIAEANQVLARLASGKLSGPAPQGRDWISDDG 257 F LPAL+G R Y+PE+P +L K A QVL R+A +G Sbjct: 183 FVTGLPALVGVVLRFYVPESPMYLNRSGKSDAARQVLERVA---------------KVNG 227 Query: 258 SRQQTAPAKVRSME------IFQGALARRTLPLCIAIWMTYGAQISVLTLMPTILVSLGY 311 S P + +E +F AL RR+L L +A + A V +P L S G+ Sbjct: 228 SSTPIPPLQPEVVERKPMSALFSAALRRRSLSLYLAWGLISIAYYGVFVYLPVKLGSEGF 287 Query: 312 TISKSLLFTMVMQSGSLLGAIAASVFGFHLPRKRVLTAGAICACLAALAIGFLAKSIVLI 371 + +F + + L G A S +G ++ G + + L K +L+ Sbjct: 288 GFMRGQIFLIFLALVQLPG-YALSAYGVERWGRKPTLVGFLVLSAVGCMLYSLGKDPMLV 346 Query: 372 LAAGAVFQFFVLLLNTTIWIFAPELYPTRVRAFG 405 + + + F +L ++ F PE+YPT +RA G Sbjct: 347 VGSTLLLSFSLLGTWAALYAFTPEVYPTDLRASG 380 >gi|300310615|ref|YP_003774707.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 110 bits (276), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 128/499 (25%), Positives = 215/499 (43%), Gaps = 71/499 (14%) Query: 6 LSSPDLPHSPRVAELDGALDKIGVTRSHHTIIFLILIGCLFDAFEQNAIGLVGPLLKQQW 65 L+S D + R A L ++ + +R H ++ LFDAF ++ V P+L W Sbjct: 18 LTSDD---AHRAAGLLFRIENVPFSRWHTKARIIMGSATLFDAFHALSLAFVLPVLVGLW 74 Query: 66 GLTATDIGLLNTVTFASAAIGRIVSGLVADRYGRRVMLSLDLLLFTLGAGICALAPDLTV 125 L+A IGLL + IG + G +A+RYGR S+ + L ++ + +CAL + Sbjct: 75 QLSAAQIGLLIASGYLGQVIGALFFGWMAERYGRVPSASVTVGLMSVLSIVCALTGSFHM 134 Query: 126 MAVGRAIVGFGLGGEIAIAVTMLAEFCSSKFRGTAVGLVN-------VGAGGMGNFLAPA 178 + + R + G G+GGE+ +A T + E + RG L + A +G FL P Sbjct: 135 LFLCRFLQGIGVGGEVPVAATYINELSQAHGRGKFFVLYELIFPLGLLAAAQVGAFLVPR 194 Query: 179 FGLLVFTLFPGENSWRWLFFCLMLPALLGAFYRRYIPETPRFLLSRNKIAEANQVLARLA 238 FG W ++F +P +L A +PE+PR+L++R ++ +A +++ L Sbjct: 195 FG------------WEYMFLVGGIPGILVALLITRLPESPRWLIARGRLDDAEKLIRTLE 242 Query: 239 SG--KLSGPAPQGRDWISDD--------GSRQQTAPAKVRSMEIFQGALARRTLPLCIAI 288 + K + A + I G RQ+ + E+F RT I + Sbjct: 243 ASTPKRNLDAQTDKQAIEQRINALAAGAGKRQRGSWK-----ELFSPVYRGRT----IIV 293 Query: 289 WMTYGAQISVLTLMPTILVSLGYTI-----SKSLLFTMVMQSGSLLGAIAASVFGFHLPR 343 W+ + + V + L SL T+ +SL V S + ++ + R Sbjct: 294 WLLWASSYFVANGINNWLPSLYKTVYHLPLQESLRMASVSNVLSACAVLGCALLIDRVGR 353 Query: 344 KRVLTAGAICACL-----------AALAIGFLAKSIVLILAAGAVFQFFVLLLNTTIWIF 392 +R A I + +A ++ FLA S ++ V ++++ Sbjct: 354 RRWAIASFILSGALLALLGVLKAESAWSVMFLASSAYAVMGTTTVL----------LYLY 403 Query: 393 APELYPTRVRAFGTAFILATGTAAGALMPLVAGRLFDAFGLAGVFGMIAAMYGIFTVAIR 452 PE+YPTR+RA GT + AA A P + G + G+A VF M A + + +A Sbjct: 404 TPEIYPTRMRAIGTGLATSWLRAASAAAPAMVGVVLAGHGIATVFLMFAGVTVLGLLAAW 463 Query: 453 TVPETYGKSPDAPLEETEP 471 + ET +S LEE P Sbjct: 464 RMIETTNRS----LEEISP 478 >gi|300313879|ref|YP_003777971.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 413 Score = 110 bits (274), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 116/441 (26%), Positives = 187/441 (42%), Gaps = 44/441 (9%) Query: 29 VTRSHHTIIFLILIGCLFDAFEQNAIGLVGPLLKQQWGLTATDIGLLNTVTFASAAIGRI 88 VT + G DA + L P L + L+ D GL++ T ++AIG Sbjct: 8 VTAKERKTFWACFSGWALDALDVQMFSLAIPALIAAFSLSKADAGLISGATLVASAIGGW 67 Query: 89 VSGLVADRYGRRVMLSLDLLLFTLGAGICALAPDLTVMAVGRAIVGFGLGGEIAIAVTML 148 +G ++DRYGR L + ++ F+ + A A + V +A+ GFG GGE A ++ Sbjct: 68 FAGALSDRYGRVKTLQITIIWFSFFTFLSAFAQSFPQLLVLKALQGFGFGGEWAAGAVLM 127 Query: 149 AEFCSSKFRGTAVGLVN----VGAGGMGNFLAPAFGLLVFTLFPGENSWRWLFFCLMLPA 204 AE ++ RG A+G V VG GG A ++F+L P E +WR +F +LPA Sbjct: 128 AETIRAEHRGKAMGTVQSAWAVGWGG-----AVLLFSVLFSLLPQEMAWRVMFVIGLLPA 182 Query: 205 LLGAFYRRYIPETPRFLLSRNKIAEANQVLARLASGKLSGPAPQGRDWISDDGSRQQTAP 264 LL + RR + ET FL + A L Sbjct: 183 LLVVYVRRSVQETDAFLAGQKAAASKPAASTLLG-------------------------- 216 Query: 265 AKVRSMEIFQGALARRTLPLCIAIWMTYGAQISVLTLMPTILVSLGY-TISKSLLFTMVM 323 IF+ + R T+ + +G +++T +PT L + + ++ + + V+ Sbjct: 217 -------IFRPDVLRLTIIGSLLGVGAHGGYYALMTWLPTYLKTEKHLSVLSTGGYLAVI 269 Query: 324 QSGSLLGAIAASVFGFHLPRKRVLTAGAICACLAALAIGFLAKSIVLILAAGAVFQFFVL 383 G IA+S + R+R + A+ L FL S +L G FF Sbjct: 270 IVAFWCGCIASSQLLDRIGRRRTVAFFALACVATVLCYIFLPLSDTQMLVCGFPLGFFAA 329 Query: 384 LLNTTIWIFAPELYPTRVRAFGTAFILATGTAAGALMPLVAGRLFDAFGLAGVFGMIAAM 443 + ++ ELYP+ +R G F G A A P++ G + D+ L G+ AA+ Sbjct: 330 GIPASMGALFNELYPSGIRGTGVGFCYNFGRVASAGFPVLVGHMSDSMPLGQAIGIDAAI 389 Query: 444 -YGIFTVAIRTVPETYGKSPD 463 Y + VA+ +PET G+ + Sbjct: 390 AYSLVVVAVLMLPETKGRKME 410 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.141 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,463,979 Number of extensions: 64123 Number of successful extensions: 481 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 463 Number of HSP's successfully gapped: 22 Length of query: 482 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 393 Effective length of database: 1,198,455 Effective search space: 470992815 Effective search space used: 470992815 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)