BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0022.0133.p
         (243 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309641|ref|YP_003773733.1| heat-shock Hsp70 protein [Herba...   367   e-103
gi|300312141|ref|YP_003776233.1| molecular chaperone, Hsp70 fami...    62   5e-11
gi|300312439|ref|YP_003776531.1| molecular chaperone protein [He...    55   4e-09

>gi|300309641|ref|YP_003773733.1| heat-shock Hsp70 protein
           [Herbaspirillum seropedicae SmR1]
          Length = 418

 Score =  367 bits (941), Expect = e-103,   Method: Compositional matrix adjust.
 Identities = 192/213 (90%), Positives = 201/213 (94%)

Query: 1   MANACGVDFGTSNSTVGWMRPQAGDSLLLPLEDGKATLPSVVFFNADENSFSYGRAGLGE 60
           MANACGVDFGTSNSTVGW+RP A DSLLLPLEDGKATLPSVVFFNADEN FSYGRAGLG+
Sbjct: 1   MANACGVDFGTSNSTVGWLRPDAPDSLLLPLEDGKATLPSVVFFNADENQFSYGRAGLGD 60

Query: 61  YLAGYEGRLMRSLKSLLGSGAIDGQTEVGGRALPYRGLLSQFIGELKQRAERAAGTSFSK 120
           YLAGYEGRLMRSLKSLLGSGAIDGQTEVGGRALPYRGLLSQFIGELK RA+RAAGTSFS 
Sbjct: 61  YLAGYEGRLMRSLKSLLGSGAIDGQTEVGGRALPYRGLLSQFIGELKMRAQRAAGTSFSS 120

Query: 121 VVLGRPVHFVDEDAAADQLAQDTLEEIARDVGFKDIAFQFEPIAAAFDYESRIDKEELVL 180
           VVLGRPVHFVD+D  ADQLAQDTLE+IARDVGFKDIAFQFEPIAAAFDYESRID+EELVL
Sbjct: 121 VVLGRPVHFVDDDDQADQLAQDTLEQIARDVGFKDIAFQFEPIAAAFDYESRIDREELVL 180

Query: 181 IVDIGGGTSDFSLVRLSPERAARSERAXYICAS 213
           IVDIGGGTSDFSLVRLSP+RA R+ER   I A+
Sbjct: 181 IVDIGGGTSDFSLVRLSPQRAQRAERRDDILAN 213


>gi|300312141|ref|YP_003776233.1| molecular chaperone, Hsp70 family
           [Herbaspirillum seropedicae SmR1]
          Length = 642

 Score = 62.0 bits (149), Expect = 5e-11,   Method: Compositional matrix adjust.
 Identities = 69/222 (31%), Positives = 96/222 (43%), Gaps = 40/222 (18%)

Query: 4   ACGVDFGTSNSTVGWMRPQAGDSLLLPLEDGKATLPSVVFF-------NADENSFSYGRA 56
           A G+D GT+NS V  +  Q+G + +LP E G+A LPSVV +        AD      G A
Sbjct: 21  AVGIDLGTTNSLVATV--QSGQARILPDEAGQALLPSVVRYLPAHSLQPADRPPAVVGHA 78

Query: 57  GLGEYLAGYEGRLMRSLKSLLGSGAIDGQTEVGGRA-LPYR-----GLL----------- 99
                 +      + S+K L+G    D     G  A +PY      GL+           
Sbjct: 79  AKAAQ-SDDPRNTLSSIKRLMGRDRAD--ISAGNSADMPYAFIDKPGLVQIDTAAGIKTP 135

Query: 100 ----SQFIGELKQRAERAAGTSFSKVVLGRPVHFVDEDAAADQLAQDTLEEIARDVGFKD 155
               +  +  L+QRAE + G   S  V+  P +F   D A  Q  +D     AR  G   
Sbjct: 136 IDVSADILRVLRQRAEASLGGELSGAVITVPAYF---DDAQRQATKDA----ARLAGLNV 188

Query: 156 IAFQFEPIAAAFDYESRIDKEELVLIVDIGGGTSDFSLVRLS 197
           +    EP AAA  Y      E +  I D+GGGT D S+++LS
Sbjct: 189 LRLLNEPTAAAIAYGLDQAAEGVFAIYDLGGGTFDISILKLS 230


>gi|300312439|ref|YP_003776531.1| molecular chaperone protein
           [Herbaspirillum seropedicae SmR1]
          Length = 621

 Score = 55.5 bits (132), Expect = 4e-09,   Method: Compositional matrix adjust.
 Identities = 60/216 (27%), Positives = 90/216 (41%), Gaps = 38/216 (17%)

Query: 4   ACGVDFGTSNSTVGWMRPQAGDSLLLPLEDGKATLPSVVFFNADENSFSYGRAGLGEYLA 63
           A G+D GT+NS V  +R    +  +L  +DG+  LPSVV +         G A +G    
Sbjct: 21  AVGIDLGTTNSLVATVRNSIPE--VLADDDGRVLLPSVVRY------LPNGHANIGYKAQ 72

Query: 64  GYEG----RLMRSLKSLLGSGAID---------------GQTE---VGGRALPYRGLLSQ 101
             +       + S+K  +G G  D               G  +   V G   P   + +Q
Sbjct: 73  AAQTTDPRNTIVSVKRFMGRGLKDIAHAENMPYDFQDQPGMVQIKTVAGVKSPVE-VSAQ 131

Query: 102 FIGELKQRAERAAGTSFSKVVLGRPVHFVDEDAAADQLAQDTLEEIARDVGFKDIAFQFE 161
            +  L+Q AE A G      V+  P +F   D A  Q  +D     A+  G   +    E
Sbjct: 132 ILATLRQTAEDALGDDLVGAVITVPAYF---DDAQRQATKDA----AQLAGLNVLRLLNE 184

Query: 162 PIAAAFDYESRIDKEELVLIVDIGGGTSDFSLVRLS 197
           P AAA  Y      E +  + D+GGGT D S+++L+
Sbjct: 185 PTAAAIAYGLDNSSEGVFAVYDLGGGTFDISILKLT 220


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.317    0.133    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 658,593
Number of extensions: 26756
Number of successful extensions: 92
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 90
Number of HSP's successfully gapped: 3
Length of query: 243
Length of database: 1,619,870
Length adjustment: 83
Effective length of query: 160
Effective length of database: 1,226,865
Effective search space: 196298400
Effective search space used: 196298400
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 129 (54.3 bits)