BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0132.p (248 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogena... 487 e-139 gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogena... 126 1e-30 gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogena... 124 1e-29 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 110 1e-25 gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herb... 107 1e-24 >gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 248 Score = 487 bits (1254), Expect = e-139, Method: Compositional matrix adjust. Identities = 242/248 (97%), Positives = 246/248 (99%) Query: 1 MSNNIKDKVVVITGASSGLGETTARHLASLGARLVLGARRTDRLEKLVADITAAGGQAIA 60 MSNNIKDKVVVITGASSGLGETTARHLASLGA+LVLGARRT+RLEKLVADITAAGGQAIA Sbjct: 1 MSNNIKDKVVVITGASSGLGETTARHLASLGAKLVLGARRTERLEKLVADITAAGGQAIA 60 Query: 61 VTTDVARRDDVEALVAKGEQHFGRIDVLVNNAGIMPLAPMAKLKVEEWDRMIDVNVKGVL 120 VTTDVARRDDVEALVAKGEQHFGRIDVLVNNAGIMPLAPMAKLKVEEWDRMIDVNVKGVL Sbjct: 61 VTTDVARRDDVEALVAKGEQHFGRIDVLVNNAGIMPLAPMAKLKVEEWDRMIDVNVKGVL 120 Query: 121 YGVAAALPRFAAQGSGHIINVSSVAGIKVFAGMGTVYSATKFAVRALTEGLRAEAPDGVR 180 YGVAAALPRFAAQ SGHIINVSSVAGIKVFAGMGTVYSATKFAVRALTEGLR EAPDGVR Sbjct: 121 YGVAAALPRFAAQSSGHIINVSSVAGIKVFAGMGTVYSATKFAVRALTEGLRTEAPDGVR 180 Query: 181 TTIISPGAVDSELKTHSSDQETSEALLAWYEANQIPADSVARAIAYAIEQPANVDISEVV 240 TTIISPGAVDSELKTHSSD+ETSEALLAWYEANQIP+DSVARAIAYAIEQPANVDISEVV Sbjct: 181 TTIISPGAVDSELKTHSSDKETSEALLAWYEANQIPSDSVARAIAYAIEQPANVDISEVV 240 Query: 241 LRPVAQEF 248 LRPVAQEF Sbjct: 241 LRPVAQEF 248 >gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 250 Score = 126 bits (317), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 84/243 (34%), Positives = 128/243 (52%), Gaps = 6/243 (2%) Query: 9 VVVITGASSGLGETTARHLASLGARLVLGARRTDRLEKLVADITAAGGQAIAVTTDVARR 68 VV+ITGA++G G AR A G +++ RR +RL+ LV ++ A + + + DV + Sbjct: 2 VVLITGATAGFGAAMARKFAGNGHKVIAAGRRKERLDDLVKELGAD--KVLPLVLDVTSK 59 Query: 69 DDVEALVAKGEQHFGRIDVLVNNAGI-MPLAPMAKLKVEEWDRMIDVNVKGVLYGVAAAL 127 +E +A + +DVL+NNAG+ + L P ++ + +W+ MID NVKG++ A L Sbjct: 60 SSIETGLASLTGAWKEVDVLINNAGLALGLGPAHEVALSDWETMIDTNVKGLVTITHALL 119 Query: 128 PRFAAQGSGHIINVSSVAGIKVFAGMGTVYSATKFAVRALTEGLRAE-APDGVRTTIISP 186 P +G G IIN+ SVAG + G G VY ATK V T LRA+ GVR T ++P Sbjct: 120 PDMVKRGRGTIINLGSVAGAYPYPG-GNVYGATKAFVDQFTLNLRADLVGTGVRATNLAP 178 Query: 187 GAV-DSELKTHSSDQETSEALLAWYEANQIPADSVARAIAYAIEQPANVDISEVVLRPVA 245 G +E + A + + AD +A A + PA+V+I+ + L P Sbjct: 179 GLCGGTEFSNVRFKGDDGAAAKVYEGTTPLTADDIAEAAFWVATMPAHVNINYMELMPTC 238 Query: 246 QEF 248 Q F Sbjct: 239 QGF 241 >gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 124 bits (310), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 74/196 (37%), Positives = 119/196 (60%), Gaps = 9/196 (4%) Query: 1 MSNNIKDKVVVITGASSGLGETTARHLASLGARLVLGARRTDRLEKLVADITAAGGQAIA 60 M+ +DKVVV+TG S G+G TA+ A+ GAR+ + RR D+LE+ V +I G A+ Sbjct: 3 MAKRFEDKVVVVTGGSEGIGLATAKLFAAEGARVYVSGRRADKLEQAVREI---GNGAVG 59 Query: 61 VTTDVARRDDVEALVAKGEQHFGRIDVLVNNAGIMPL--APMAKLKVEEWDRMIDVNVKG 118 V D A+ DD++ L A+ + GR+DVL NAG P+ + E++D + ++NV+G Sbjct: 60 VPGDAAKLDDLDRLYARVQADHGRLDVLFANAGTASTEAKPLGMIGEEDFDGLFNLNVRG 119 Query: 119 VLYGVAAALPRFAAQGSGHIINVSSVAGIKVFAGMGTVYSATKFAVRALTEGLRAEAPD- 177 +L+ V ALP +QG ++N SVAG+K F G ++Y+A+K AVR+ A+ + Sbjct: 120 LLFSVQKALP-LMSQGGAIVLN-GSVAGVKGFPGQ-SLYNASKAAVRSFARSWTADLKER 176 Query: 178 GVRTTIISPGAVDSEL 193 G+R +++PG ++ L Sbjct: 177 GIRVNVVAPGGTETRL 192 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 110 bits (274), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 72/209 (34%), Positives = 108/209 (51%), Gaps = 4/209 (1%) Query: 3 NNIKDKVVVITGASSGLGETTARHLASLGARLVLGARRTDRLEKLVADITAAGGQAIAVT 62 N ++ KV +ITGAS+G+G TA A GA L+L ARR D LE++ A I A GG+A V Sbjct: 2 NTLQGKVALITGASAGIGRATALLFARHGAALILTARRQDALEQVAALICAQGGRASVVA 61 Query: 63 TDVARRDDVEALVAKGEQHFGRIDVLVNNAG-IMPLAPMAKLKVEEWDRMIDVNVKGVLY 121 DV E VA FG +D+ +NNAG + P+A++ ++W + N+ Sbjct: 62 GDVGEAATHERGVAMALDEFGGLDIAINNAGAVGACKPLAEISPQQWQDTLHANLTSAFL 121 Query: 122 GVAAALPRFAAQGSGHIINVSSVAGIKV-FAGMGTVYSATKFAVRALTEGLRAE-APDGV 179 G +P A+G+G I+ SS G GM Y A K + L +G+ A+ A G+ Sbjct: 122 GARCQIPAMLARGAGAIVFTSSFVGSSAGLPGM-AAYGAAKAGLMGLVKGIAADYAVQGI 180 Query: 180 RTTIISPGAVDSELKTHSSDQETSEALLA 208 R + PG VD+++ ++ + L A Sbjct: 181 RANALLPGGVDTDMGGDPQQKQWAAGLHA 209 >gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 107 bits (266), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 73/229 (31%), Positives = 124/229 (54%), Gaps = 9/229 (3%) Query: 2 SNNIKDKVVVITGASSGLGETTARHLASLGARLVLG-ARRTDRLEKLVADITAAGGQAIA 60 ++ + KV + G S G+G + LA+ GA + D+ + LV+ + A GG+A+A Sbjct: 12 TSTLVSKVAFVQGGSRGIGAAIVQRLAAEGATVAFTYVSSPDKAQALVSSVQAKGGRALA 71 Query: 61 VTTDVARRDDVEALVAKGEQHFGRIDVLVNNAGIMPLAPMAKLKVEEWDRMIDVNVKGVL 120 + D A ++ + + FGR+D+LVNNAG++ + P+ + K+E+ DR + VNV+ V Sbjct: 72 IQADSADAAALQQAIRLAAETFGRLDILVNNAGVLAMGPLDQFKLEDLDRTLAVNVRSVF 131 Query: 121 YGVAAALPRFAAQGSGHIINVSSVAGIKV-FAGMGTVYSATKFAVRALTEGL-RAEAPDG 178 A R+ +G G +I++ S ++ FAG G VY+ +K A+ LT+GL R P G Sbjct: 132 VATQEA-ARYMGEG-GRVISIGSTNADRIPFAG-GAVYAMSKSALVGLTKGLARDLGPRG 188 Query: 179 VRTTIISPGAVDSELKTHSSDQETS-EALLAWYEANQIPADSVARAIAY 226 + + PG VD+++ S D S L+A + A+ +A +AY Sbjct: 189 ITVNNVQPGPVDTDMNPASGDFAASLTGLMALPRYGK--AEEIASFVAY 235 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.131 0.359 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 750,883 Number of extensions: 31540 Number of successful extensions: 256 Number of sequences better than 1.0e-08: 48 Number of HSP's gapped: 190 Number of HSP's successfully gapped: 48 Length of query: 248 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 165 Effective length of database: 1,226,865 Effective search space: 202432725 Effective search space used: 202432725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)