BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0121.p (565 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme I... 229 6e-61 gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbasp... 135 7e-33 gi|300310483|ref|YP_003774575.1| benzoylformate decarboxylase [H... 115 9e-27 gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspi... 93 6e-20 gi|300313238|ref|YP_003777330.1| acetolactate synthase [Herbaspi... 61 2e-10 >gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme III large subunit [Herbaspirillum seropedicae SmR1] Length = 572 Score = 229 bits (583), Expect = 6e-61, Method: Compositional matrix adjust. Identities = 162/570 (28%), Positives = 275/570 (48%), Gaps = 33/570 (5%) Query: 1 MTGADVLVKLLEEYGVEVIFGVPGDTNVAFYKALNRSGKVRHVMARDERSALYMADAYAR 60 +TGAD+LV+ L E GVE +FG PG + Y A+ + K +H++ R E++A++ ADAY+R Sbjct: 6 LTGADILVRCLAEEGVEHVFGYPGGAVLYIYDAIFKQDKFQHILVRHEQAAVHAADAYSR 65 Query: 61 YSGKPGVAECPSGAGAMYSLPGMAEANSSSIPLILLTNDIPLKGEGRGMITELDNAKLFE 120 S K GVA SG G ++ G+A A SIP+++++ +P G+ E D + Sbjct: 66 SSQKVGVAIVTSGPGVTNAVTGLATAYMDSIPMVVISGQVPSTAIGQDAFQECDTVGITR 125 Query: 121 PVCKLSFQVKTTAKIPEAVRRAFRTATSGRPGAVHLTFPEDILAHASASTPDEIYAEPEC 180 P K +F VK + E V++AF AT+GRPG V + P+DI H A YA P+ Sbjct: 126 PCVKHNFLVKDVKDLAETVKKAFYIATTGRPGPVLVDIPKDITMHTCA------YAYPKA 179 Query: 181 RRFPAF-PM-RPDADLVRQ-----LAPRRPVLVAGGGVMHSDGGAALLALAEKIDAAVVT 233 ++ P+ + + +R+ L+ RP++ GGG++ ++ L L +++ Sbjct: 180 VEMRSYKPVDKGHSGQIRKALQLLLSAERPMIYTGGGIILANAAPELNKLVDRLGYPCTN 239 Query: 234 TITGQGAVPDEHPLALGVIGDNGFHPHAARAVEESDLLVYLGCKMGS-VVTIGWTFPSHQ 292 T+ G GA +G+ G +G + A A++ D+L+ +G + V+ F SH Sbjct: 240 TLMGLGAYRSSSDKFVGMPGMHGTY-EANMAMQHCDVLIAIGARFDDRVIGNPKHFASH- 297 Query: 293 QERQRVQVDIDPAVLGNTGRNDLNIAGDVRSVLQDLLPLVTQHEPRAWNAELNGMRRRFW 352 R+ + +DIDP+ + + D+ I G+V+ VLQ+LL + E R A L+ +W Sbjct: 298 -ARKIIHIDIDPSSISKRVKVDIPIVGNVKDVLQELLSQLDAAETRQNGAALDA----WW 352 Query: 353 EQAQADLHSDALP-------LKPQRVVHELNRRLPPRAVMVSDAGTPTPHMTRYLRLPGD 405 +Q D L +KPQ VV ++ A + SD G +Y Sbjct: 353 KQINEWRKRDCLKFATSEEFIKPQSVVQKVWEVTKGDAFITSDVGQHQMWAAQYYGFD-K 411 Query: 406 GSRLVIPRAFGGLGYAIPAVVGAWLARPDVRPIGLFGDGSFSMSCGELETLTRLQVPAVL 465 R + G +G +P +G +A PD + G+ S M EL T + + + Sbjct: 412 PRRWINSGGLGTMGVGLPYAMGVQMANPDATVACITGEASIQMCIQELATCKQYHLTPKI 471 Query: 466 LHFNNGCFGWIKALQKLHNDAQYLSVDFTRL-DVARIAQGFGLKGLRAANAEELVAALDE 524 + NN G ++ Q++ ++Y L D ++A+ +G G++ ++ AL E Sbjct: 472 ILLNNRFLGMVRQWQQIDYGSRYSESYMDSLPDFNKLAESYGHVGMKIEKPGDVDGALRE 531 Query: 525 AFASEGPVLIDIVSESIVDECPPVYSWIKA 554 AFA + + + I D+ V+ +KA Sbjct: 532 AFAMKDRL---VFMNFITDQTENVWPMVKA 558 >gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 564 Score = 135 bits (341), Expect = 7e-33, Method: Compositional matrix adjust. Identities = 142/552 (25%), Positives = 231/552 (41%), Gaps = 26/552 (4%) Query: 2 TGADVLVKLLEEYGVEVIFGVPGDTNVAFYKALNRSGKVRHVMARDERSALYMADAYARY 61 T AD+L+ L E GV IFGV GD A+ R ++ + R E A A A+ Sbjct: 4 TVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAAGQAKL 63 Query: 62 SGKPGVAECPSGAGAMYSLPGMAEANSSSIPLILLTNDIPLKGEGRGMITELDNAKLFEP 121 +G+ V +G GA + + G+ EA P++ ++ +P G + E LF Sbjct: 64 TGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELLFRD 123 Query: 122 VCKLSFQVKTTAKIPEAVRRAFRTATSGRPGAVHLTFPEDIL-AHASASTPD-EIYAEPE 179 V S + + + P +A A + R G HL P D++ A S S P E Sbjct: 124 VAAYSQIITSPDQAPAVFHQAIAQAYAQR-GVAHLNIPADVIGAKTSGSVPSLHTLRERT 182 Query: 180 CRRFPAFPMRPDADLVRQLAPRRPVLVAGGGVMHSDGGAALLALAEKIDAAVVTTITGQG 239 PA ++ ADL+ A + AG G + +LALA K+ A V+ T G+ Sbjct: 183 PLSPPAADIQAAADLIN--AAGSVAIFAGNGCRGAID--EVLALASKLQAPVMHTFRGKD 238 Query: 240 AVPDEHPLALGVIGDNGFHPHAARAVEESDLLVYLGCKMGSVVTIGWTFPSHQ---QERQ 296 +HP +G +G G P A+ +++++ LG +P + + + Sbjct: 239 LASYDHPHWIGGVGLIGGAP-GTDALHAAEVMLMLGSD----------YPYSEFLPTKSK 287 Query: 297 RVQVDIDPAVLGNTGRNDLNIAGDVRSVLQDLLPLVTQHEPRAWNAELNGMRRRFWEQ-- 354 +Q+D VLG L I G V +Q LL V A+ + N R R+ + Sbjct: 288 VIQIDERGFVLGRRAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLD 347 Query: 355 AQADLHSDALPLKPQRVVHELNRRLPPRAVMVSDAGTPTPHMTRYLRLPGDGSRLVIPRA 414 + A++ +KPQ V ++ A V D G T ++R G G R++ Sbjct: 348 SAAEIKRHHKKIKPQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRG-GQRILASFN 406 Query: 415 FGGLGYAIPAVVGAWLARPDVRPIGLFGDGSFSMSCGELETLTRLQVPAVLLHFNNGCFG 474 +G ++ G + + + GDG F+M GE T ++P ++ FNN +G Sbjct: 407 NAAVGTSLGQANGIQALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWG 466 Query: 475 WIKALQKLHNDAQYLSVDFTRLDVARIAQGFGLKGLRAANAEELVAALDEAFASEGPVLI 534 + + + +F D A A+ G +G EEL L + A+ GP ++ Sbjct: 467 LVHLEMEEAGMPAFEGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVL 526 Query: 535 DIVSESIVDECP 546 D+ + DE P Sbjct: 527 DVFIDP--DELP 536 >gi|300310483|ref|YP_003774575.1| benzoylformate decarboxylase [Herbaspirillum seropedicae SmR1] Length = 532 Score = 115 bits (288), Expect = 9e-27, Method: Compositional matrix adjust. Identities = 147/543 (27%), Positives = 228/543 (41%), Gaps = 39/543 (7%) Query: 7 LVKLLEEYGVEVIFGVPGDTNVAFYKALNRSGKVRHVMARDERSALYMADAYARYSGKPG 66 ++ L+ G+ +F PG T + ++ R+V+ E + MAD +A+ SG Sbjct: 15 VLDLMRRLGMTSVFANPGSTELPMFRDFPE--DFRYVLGLQEAVVVGMADGFAQASGNAA 72 Query: 67 VAECPSGAGAMYSLPGMAEANSSSIPLILLTNDIPLKGEGRGMITELD-------NAKLF 119 + S AG ++ + A + PLI+ G+ I D +L Sbjct: 73 LVNLHSAAGVGNAMGNIFTAFRNRTPLIITA------GQQARSILPFDPFLGATQATELA 126 Query: 120 EPVCKLSFQVKTTAKIPEAVRRAFRTATSGRPGAVHLTFP-EDILAHASASTPDEIYAEP 178 +P K S + A +P A+ RA+ A G V ++ P +D A P ++ + Sbjct: 127 KPYLKWSIEPARAADVPLALARAYHMAMQEPRGPVFVSIPVDDWDQPAELVPPTQVTSR- 185 Query: 179 ECRRFPAFPMRPDADLVRQLAPRRPVLVAGGGVMHSDGGAALLALAEKIDAAV-VTTITG 237 CR PA R A L + +RP +V G + + A +ALAE + A V V ++ Sbjct: 186 -CRPEPAAIARLAAALEQS---QRPAIVVGAELDRAGAWDAAIALAETLRARVFVAPMSS 241 Query: 238 QGAVPDEHPLALGVIGDNGFHPHAARAVEESDLLVYLGCKMGSVVTIGWTFPSHQQERQR 297 + + P++HPL G + +A++ DLL LG + G + P Sbjct: 242 RCSFPEDHPLFAGFL--PAMREKIVQALQGHDLLFALGAPAFTYHVEG-SGPHVPPGAAL 298 Query: 298 VQVDIDPAVLGNTGRNDLNIAGDVRSVLQDLLPLVTQHEPRAWNAELNGMRRRFWEQAQA 357 Q+ DP T I G+++ L DLL + + +P A+ R +A A Sbjct: 299 FQLIDDPVTASWTPTGQ-AIVGNIQLGLDDLLAQI-RRQPPTQRAQPPARPARARVEASA 356 Query: 358 DLHSDALPLKPQRVVHELNRRLPPRAVMVSDAGTPTPHMTRYLRLPGDGSRLVIPRAFGG 417 L S A L+ V E ++V +A P M +L GS + A GG Sbjct: 357 PL-SMAYALQTLADVREAGD------IVVEEAPGSRPVMQAHLPFTRPGSFYTM--ASGG 407 Query: 418 LGYAIPAVVGAWLARPDVRPIGLFGDGSFSMSCGELETLTRLQVPAVLLHFNNGCFGWIK 477 LGY +PA VG LARP R IGL GDGS S + + +L +P + NNG + ++ Sbjct: 408 LGYGMPAAVGVALARPHSRVIGLIGDGSAMYSPQAIYSAVQLGLPITFVILNNGRYAALQ 467 Query: 478 ALQKLHNDAQYLSVDFTR---LDVARIAQGFGLKGLRAANAEELVAALDEAFASEGPVLI 534 + V T LD +A G G+R + A L AL EA S L+ Sbjct: 468 DFAPVFGFGPEDPVHGTELPGLDFVALAHSMGCHGVRVSQAAALAPALREALCSNETRLV 527 Query: 535 DIV 537 +IV Sbjct: 528 EIV 530 >gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspirillum seropedicae SmR1] Length = 625 Score = 92.8 bits (229), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 138/584 (23%), Positives = 253/584 (43%), Gaps = 55/584 (9%) Query: 1 MTGADVLVKLLEEYGVEV----------IFGVPGDTNVA-FYKALNR-SGKVRHVMARDE 48 +T A LV+ L+ ++ +F + G NVA +AL + + A +E Sbjct: 13 LTMAQALVRYLDALRIQTPQGVLPLFGGVFSIFGHGNVAGMGEALYQYRATLPTYRAHNE 72 Query: 49 RSALYMADAYARYSGKPGVAECPS--GAGAMYSLPGMAEANSSSIPLILLTNDIPLKGEG 106 ++ + A AYA+ + + + G GA L A A+ + +P++LL D+ + Sbjct: 73 QAMAHSAIAYAKAHLRQRMMAVTTSIGPGATNLLTAAALAHVNRLPVLLLPGDVFVSRRP 132 Query: 107 RGMITELDNAK--------LFEPVCKLSFQVKTTAKIPEAVRRAFRTATS-GRPGAVHLT 157 ++ +L++ F P+ ++ ++ ++ A+ RA + T + G V L Sbjct: 133 DPVLQQLEDFNDPGLSVNDAFRPLSRMFDRICYPEQLLTALPRAIQVLTDPAQCGPVTLA 192 Query: 158 FPEDILAHASASTPDEIYAE----PECRRFPAFPMRPDADLVRQLAPRRPVLVAGGGVMH 213 P+D+ A P + ++ P + A + L+RQ R+P+L+AGGGV++ Sbjct: 193 LPQDVQTMAY-DYPLDFFSPRVIVPRAQAPAAQELEDAVALLRQ--ARQPLLIAGGGVLY 249 Query: 214 SDGGAALLALAEKIDAAVVTTITGQGAVPDEHPLALGVIGDNGFHPHAARAVEESDLLVY 273 + L AE+ V T G+ A+P H L +G IG G P A +E+D+++ Sbjct: 250 GNACEHLRRFAEQHGVPVAETQAGKSALPWSHALQMGAIGVTG-SPAANALAQEADVVIA 308 Query: 274 LGCKMGSVVTIGWTFPSHQQERQRVQVDIDPAVLGNTGRNDLNIAGDVRSVLQDLL-PLV 332 +G ++ +T SH Q ++++ + L + D L+ L L Sbjct: 309 VGTRLQD-----FTTGSHTLFAQARLLNLNVNAMDAHKWRGLALQADAGLGLEALSHALE 363 Query: 333 TQHEPRAWNAELNGMRRRFWEQ-AQADLHSDA---LPLKPQRVVHELNRRL---PPRAVM 385 W+A + + + + E+ AQ +D LP + V+ + R + P + ++ Sbjct: 364 GWRSAPEWHARAHTLAQDWRERVAQITGQTDTGGRLPYDGE-VIGAIQRSVADSPSQDIV 422 Query: 386 VSDAGTPTPHMTRYLRLPGDGSRLVIPRAFGGLGYAIPAVVGAWLARPDVRPIGLFGDGS 445 V AGT + + R G+ V + +GY + +G LA+P I + GDGS Sbjct: 423 VCAAGTLPAELHKLWRAGRPGAYHV-EYGYSCMGYEVAGGLGVKLAQPQREVIVIVGDGS 481 Query: 446 FSMSCGELETLTRLQVPAVLLHFNNGCFGWIKALQKLHNDAQY--LSVDFTR-------L 496 + M EL T L +++ +N +G I LQ+ A + + D + + Sbjct: 482 YLMMNSELATSVMLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNMLADCLQAGPGAPAI 541 Query: 497 DVARIAQGFGLKGLRAANAEELVAALDEAFASEGPVLIDIVSES 540 D A A+ G G EL AAL A A++ L+ I +++ Sbjct: 542 DFAAHARSLGALGENVKTITELEAALQRARAADRSYLVCIDTDA 585 >gi|300313238|ref|YP_003777330.1| acetolactate synthase [Herbaspirillum seropedicae SmR1] Length = 550 Score = 61.2 bits (147), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 54/181 (29%), Positives = 83/181 (45%), Gaps = 16/181 (8%) Query: 366 LKPQRVVHELNRRLPPRAVMVSDAGTPTPHMTRYLRLPGDGSR--LVIPRAFGGLGYAIP 423 L PQ++ L R LP RA++ + GT D +R V+ G +G +P Sbjct: 374 LTPQKIAAVLARELPERAIVSVEGGT----CGYPFYAVSDAARPHTVLTNTGGAIGQGLP 429 Query: 424 AVVGAWLARPDVRPIGLFGDGSFSMSCGELETLTRLQVPAVLLHFNNGCFGWIKALQKLH 483 +GA +A PD +GL DGS + L +L V+L N + ++ +L Sbjct: 430 VALGAAVACPDRTVVGLLSDGSTQYTIQTLWSLAHENARVVVLIAANHQYAILR--NELR 487 Query: 484 ND--------AQYLSVDFTRLDVARIAQGFGLKGLRAANAEELVAALDEAFASEGPVLID 535 D AQ ++D R+D +AQ +G+ RA A++L L AFA GP LI+ Sbjct: 488 RDGAPLGERAAQMTALDHPRIDWVGLAQSYGVPASRAETAQQLQQQLQAAFAGTGPALIE 547 Query: 536 I 536 + Sbjct: 548 M 548 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,993,274 Number of extensions: 92529 Number of successful extensions: 302 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 286 Number of HSP's successfully gapped: 6 Length of query: 565 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 475 Effective length of database: 1,193,720 Effective search space: 567017000 Effective search space used: 567017000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)