BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0119.p (336 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [... 556 e-159 gi|300310978|ref|YP_003775070.1| alkanesulfonate monooxygenase [... 328 3e-91 gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-... 119 4e-28 gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-... 65 5e-12 gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirill... 65 8e-12 >gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [Herbaspirillum seropedicae SmR1] Length = 375 Score = 556 bits (1432), Expect = e-159, Method: Compositional matrix adjust. Identities = 270/279 (96%), Positives = 276/279 (98%) Query: 1 MSLELFWFLPTSGDTRYLGKSDFGRAPTLDYMRQIAVTSEALGYDGLLIPTGASCLDPWV 60 MSLELFWFLPTSGDTRYLGKSDFGRAPTL+YMRQIAVTSE LGYDGLLIPTGASCLDPWV Sbjct: 1 MSLELFWFLPTSGDTRYLGKSDFGRAPTLEYMRQIAVTSETLGYDGLLIPTGASCLDPWV 60 Query: 61 VAASLVPVTQRIKLLVALRTSLGGPGPSARQAATLDQALNGRLLLNVVPGGDATELEADG 120 VAASLVPVTQRIKLLVALRTSLGGPGPSARQAATLDQALNGRLLLNVVPGGDATELEADG Sbjct: 61 VAASLVPVTQRIKLLVALRTSLGGPGPSARQAATLDQALNGRLLLNVVPGGDATELEADG 120 Query: 121 VFLKHDERYEAADEYLTIWRKLLQGETVNYEGKHFRIRNGQLFHPAIQQPYPPLYFGGSS 180 VFLKHDERYEAADEYLTIWRKLLQGETVNYEGKHFRIRNGQLFHPA+QQPYPPLYFGGSS Sbjct: 121 VFLKHDERYEAADEYLTIWRKLLQGETVNYEGKHFRIRNGQLFHPAVQQPYPPLYFGGSS 180 Query: 181 PAAHDLAAKHVDAYLTWGEPPAAVKQKIEDVRERAAKQGRTVRFGVRLHAIVRETTAEAW 240 PAAHDLAAKHVDAYLTWGEPPAAV+QKI+DVRERAA+QGRTVRFGVRLHAIVRETTAEAW Sbjct: 181 PAAHDLAAKHVDAYLTWGEPPAAVRQKIDDVRERAARQGRTVRFGVRLHAIVRETTAEAW 240 Query: 241 ADAERLISKLTDEDIARAQQNYAKMDSVGQQRMAALHGG 279 AERLISKLTDEDIARAQQNYA+MDSVGQQRMAALHGG Sbjct: 241 TAAERLISKLTDEDIARAQQNYARMDSVGQQRMAALHGG 279 >gi|300310978|ref|YP_003775070.1| alkanesulfonate monooxygenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 328 bits (842), Expect = 3e-91, Method: Compositional matrix adjust. Identities = 169/281 (60%), Positives = 210/281 (74%), Gaps = 4/281 (1%) Query: 3 LELFWFLPTSGDTRYLGKSDFGRAPTLDYMRQIAVTSEALGYDGLLIPTGASCLDPWVVA 62 + +FWF+PT GD+RYLG S+ RA + DYM+Q+AV ++ LGYDG+L+PTG SC D WV A Sbjct: 1 MNVFWFIPTHGDSRYLGTSEGARAVSHDYMKQVAVAADTLGYDGVLLPTGRSCEDAWVAA 60 Query: 63 ASLVPVTQRIKLLVALRTSLGGPGPSARQAATLDQALNGRLLLNVVPGGDATELEADGVF 122 ASL+ T+R+K LVA+R L P +AR AAT D+ GRLL+NVV GGD E E+DGVF Sbjct: 61 ASLIDATRRLKFLVAVRPGLMAPTLAARMAATFDRLSGGRLLINVVTGGDPVEQESDGVF 120 Query: 123 LKHDERYEAADEYLTIWRKLL----QGETVNYEGKHFRIRNGQLFHPAIQQPYPPLYFGG 178 H ERYE +DE+L IWR +L GE +++GKH ++ + +P +QQPYPPLYFGG Sbjct: 121 GSHAERYEISDEFLKIWRDVLTKTRSGEPTHFQGKHLSVKGAKALYPPVQQPYPPLYFGG 180 Query: 179 SSPAAHDLAAKHVDAYLTWGEPPAAVKQKIEDVRERAAKQGRTVRFGVRLHAIVRETTAE 238 SS A DLAA+ VD YLTWGEPPAAV +KI VRERAAK GRT+RFG+RLH IVRET Sbjct: 181 SSDEAIDLAAEQVDVYLTWGEPPAAVAEKIARVRERAAKAGRTLRFGIRLHVIVRETNEA 240 Query: 239 AWADAERLISKLTDEDIARAQQNYAKMDSVGQQRMAALHGG 279 AW A+ LIS+L DE IA+AQ N+A+MDS GQ+RMAALHGG Sbjct: 241 AWRAADELISRLDDETIAKAQANFARMDSEGQRRMAALHGG 281 >gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 119 bits (297), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 84/259 (32%), Positives = 135/259 (52%), Gaps = 14/259 (5%) Query: 28 TLDYMRQIAVTSEALGYDGLLIPT------GASCLDPWV-VAASLVPVTQRIKLLVALRT 80 +L+Y +++AVT+E G++ L GA V + +L+ T ++K+L A+ Sbjct: 42 SLEYNQRLAVTAEKAGFEYALSQIRFTAGYGAEYQHESVSFSQALLHATTKLKVLAAILP 101 Query: 81 SLGGPGPSARQAATLDQALNGRLLLNVVPGGDATELEADGV-FLKHDERYEAADEYLTIW 139 P A+Q AT+D NGR+ +NVV G E +A G +L+HDERY ++E++ Sbjct: 102 GPWHPAVVAKQIATIDHISNGRIAINVVSGWFKGEFQAIGEPWLEHDERYRRSNEFIRAL 161 Query: 140 RKLLQGETVNYEGKHFRIRNGQLFHPAIQQPYPPLYFGGSSPAAHDLAAKHVDAYLTWGE 199 + + + ++G +R N L +Q+P+P ++ GGSS AA D AA D Y T G Sbjct: 162 KGIWTQDDFTFKGDFYRFNNYTLSPKPVQKPHPEIFQGGSSRAARDNAASVSDWYFTNGN 221 Query: 200 PPAAVKQKIEDVRERAAKQGRTVRFGVRLHAIVRETTAEAWADAERLISKLTDEDIARAQ 259 +K +I+D++ +AA+ G VR GV I R+T EA A + +I D A + Sbjct: 222 TLEGLKAQIDDIQAKAAQNGHKVRIGVNAFVIARDTEEEAQAVLQDII------DHAHVE 275 Query: 260 QNYAKMDSVGQQRMAALHG 278 +A D+V Q A+ G Sbjct: 276 AVHAFGDAVKQAGKASPEG 294 >gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 65.5 bits (158), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 46/188 (24%), Positives = 84/188 (44%), Gaps = 25/188 (13%) Query: 57 DPWVVAASLVPVTQRIKLLVALRTSLGGPGPSARQAATLDQALNGRLLLNVVPGGDATEL 116 DP ++ ++ VT+ + V + P AR+ +TLD GR+ N+V G + Sbjct: 85 DPLMLVPAMAAVTEHLGFGVTANLTYEAPFLFARRMSTLDHLSRGRVGWNIVTGYLDSAA 144 Query: 117 EADGVF--LKHDERYEAADEYLTIWRKLLQG----------------------ETVNYEG 152 A G+ L+HD+RY+ ADE++ + KL +G + + G Sbjct: 145 RAMGLHEQLEHDQRYDRADEFMEVVYKLWEGSWEDGAVLRDRARRVYADPAKVHKIAHHG 204 Query: 153 KHFRIRNGQLFHPAIQQPYPPLYFGGSSPAAHDLAAKHVDAYLTWGEPPAAVKQKIEDVR 212 ++ + L P+ Q+ P LY GSS A+H + + A ++ ++ +R Sbjct: 205 DYYHVEGIHLSEPSRQRT-PVLYQAGSSGRGQAFGARHAECVFISSQSKDATRKLVQSIR 263 Query: 213 ERAAKQGR 220 + A ++GR Sbjct: 264 DGAVREGR 271 >gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirillum seropedicae SmR1] Length = 433 Score = 65.1 bits (157), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 59/191 (30%), Positives = 83/191 (43%), Gaps = 26/191 (13%) Query: 53 ASCLDPWVVAASLVPVTQRIKLLVALRTSLGGPGPSARQAATLDQALNGRLLLNVVPGGD 112 A+ LDP + +L T I L T+ P AR+ ATLD GR NVV Sbjct: 77 ATRLDPLPILGALAAATSHIGLGATRSTTYSQPYSLAREFATLDHLSGGRAAWNVVTS-- 134 Query: 113 ATELEADGVFLK----HDERYEAADEYLTIWRKL----------LQGE---------TVN 149 + EAD LK HD RY+ ADE+L + KL LQ + ++ Sbjct: 135 VNQGEADNFGLKETLPHDVRYDRADEFLEVTHKLWHSWQPDALRLQQDRYADADKVAAID 194 Query: 150 YEGKHFRIRNGQLFHPAIQQPYPPLYFGGSSPAAHDLAAKHVDAYLTWGEPPAAVKQKIE 209 ++G HF +R G L PA Q +P + GSS D AA+ + ++ Sbjct: 195 HQGAHFSVR-GPLNIPASPQGHPVIIQAGSSDRGQDFAARWAEVVFNIQPDLPRLQAFYR 253 Query: 210 DVRERAAKQGR 220 D++ RA + GR Sbjct: 254 DLKNRAQQFGR 264 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,139,151 Number of extensions: 49564 Number of successful extensions: 149 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 144 Number of HSP's successfully gapped: 5 Length of query: 336 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 250 Effective length of database: 1,212,660 Effective search space: 303165000 Effective search space used: 303165000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)