BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0112.p (571 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310913|ref|YP_003775005.1| chloride channel EriC protein [... 853 0.0 gi|300311435|ref|YP_003775527.1| chloride channel protein [Herba... 108 1e-24 gi|300311782|ref|YP_003775874.1| chloride channel protein [Herba... 62 2e-10 gi|300310046|ref|YP_003774138.1| chloride-channel protein [Herba... 56 9e-09 >gi|300310913|ref|YP_003775005.1| chloride channel EriC protein [Herbaspirillum seropedicae SmR1] Length = 572 Score = 853 bits (2203), Expect = 0.0, Method: Compositional matrix adjust. Identities = 490/571 (85%), Positives = 520/571 (91%), Gaps = 1/571 (0%) Query: 1 MKHKRDYTADRRLPLLCAIAAAIGVLSTGAAWLLLKLIALFTNLFYFQRLSFEAISPAAH 60 MKHKRDYTADRRLP LCAIA IG+LSTGAAWLLLKLIALFTNLFYFQRLS A +PA H Sbjct: 1 MKHKRDYTADRRLPYLCAIALPIGLLSTGAAWLLLKLIALFTNLFYFQRLSLAASTPATH 60 Query: 61 TLGWMAALLPVIGGLIVGLMARYGSDKIRGHGIPEAIEAILFGKSRMSPKVALLKPLSSG 120 TLGW+AALLP+ GGLIVGLMARYGSDKIRGHGIPEAIEAILFGKSRMSPKVALLKPLSSG Sbjct: 61 TLGWIAALLPIAGGLIVGLMARYGSDKIRGHGIPEAIEAILFGKSRMSPKVALLKPLSSG 120 Query: 121 IVIGSGGPFGAEGPIIMTGGSLGSLLAQWLHLTAAERKTLLVAGACAGMTAIFGTPLAAL 180 IVIGSGGPFGAEGPIIMTGGS+GSLLAQ LHLTAAERKTLLVAGACAGMTAIFGTPLAA+ Sbjct: 121 IVIGSGGPFGAEGPIIMTGGSIGSLLAQLLHLTAAERKTLLVAGACAGMTAIFGTPLAAV 180 Query: 181 LLAVELLLFELRPRSLLPVALACAIAGFLRPWLIEGGVLFPLEVAPVAPIALLSCLAAGL 240 LLAVELLLFEL+PRSLLPVALACA+AGF+RPWL+E G LFPL+VA P+ALLSCL AGL Sbjct: 181 LLAVELLLFELKPRSLLPVALACAVAGFVRPWLVEAGPLFPLDVAAPTPVALLSCLVAGL 240 Query: 241 ASGVLAALLTLALYRIEDGFHRLPLHWMWWPALGGIAVGLGGLLQPRALGVGYDVIADLL 300 ASGVLAA LTLALYRIEDGFHRL LHWMWWPALGGIAVGLGGLLQPRALGVGYDVI DLL Sbjct: 241 ASGVLAAALTLALYRIEDGFHRLRLHWMWWPALGGIAVGLGGLLQPRALGVGYDVIGDLL 300 Query: 301 NQHMLVSAALALLLVKAVIWLLALGSGTSGGVLAPLLMMGAGLGLVLAPWLPGGSPGLWP 360 +QHML+SAALALLLVKAVIWLLALGSGTSGGVLAPLLM+GAGLGLVL+PWLPGGS GLWP Sbjct: 301 HQHMLLSAALALLLVKAVIWLLALGSGTSGGVLAPLLMLGAGLGLVLSPWLPGGSDGLWP 360 Query: 361 LVCMAGVLGSVLGAPLTAIVFAFGLTHAGNALLPLIATTAVAYGVNVLWMKRSIMTEKIA 420 LVCMAGVLGSVLGAPLTAIVFAFGLTHA ALLPLIATTAVAYGVNVL MKRSIMTEKIA Sbjct: 361 LVCMAGVLGSVLGAPLTAIVFAFGLTHASQALLPLIATTAVAYGVNVLLMKRSIMTEKIA 420 Query: 421 RRGLHVHREYGVDPLERVHVEDLMS-APQAVEAALPAGEALARYYIIERVHRYYPVTAHG 479 RRGLHVHREYGVDPLER+HVE+LM+ APQ + ALPA EALA E VHRYYPVT Sbjct: 421 RRGLHVHREYGVDPLERMHVEELMTAAPQTLAGALPAAEALAWCRAQEHVHRYYPVTDGV 480 Query: 480 RLLGMVALSRLQFADPAEGCGTLMQADAGYLLPHLSARAAAGAMARQGVARLPVVNGPER 539 L+GM+AL L+ AD ++ C TL Q YLLPH+SAR+AAG MAR GVARLPVV GPER Sbjct: 481 HLVGMLALRALEHADASQPCDTLAQGHEHYLLPHMSARSAAGMMARLGVARLPVVAGPER 540 Query: 540 RQLVGVLSLSDLARASASHAEEESVREKLLG 570 R+LVGVLSLSDLARASA+HAEEESVRE+LLG Sbjct: 541 RKLVGVLSLSDLARASAAHAEEESVRERLLG 571 >gi|300311435|ref|YP_003775527.1| chloride channel protein [Herbaspirillum seropedicae SmR1] Length = 555 Score = 108 bits (271), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 146/517 (28%), Positives = 230/517 (44%), Gaps = 43/517 (8%) Query: 64 WMAALLPVIGGLIVGLMARYGSDKIRGHGIPEAIEAILFGKSRMSPKVALLKPLSSGIVI 123 + LLP GG++ GL + G+G + +EA+ G + + LL+ +SS I Sbjct: 47 YTRVLLPTCGGVVAGLFLVWAKRTPAGNG-GDYMEAVAIGDGAIPVRNTLLRSISSLASI 105 Query: 124 GSGGPFGAEGPIIMTGGSLGSLLAQWLHLTAAERKTLLVAGACAGMTAIFGTPLAALLLA 183 SGG G EGP++ SL+ ++ H ++ + L+ GA AG+T+ + P+A Sbjct: 106 ASGGSIGREGPMVQLSALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGAFFI 165 Query: 184 VELLLFELRPRSLLPVALACAIAGFLRPWLIEGGVLFPLEVAP-VAPIALLSCLAAGLAS 242 E++L L S PV +A +A L + + P + ++ L G+ + Sbjct: 166 TEIVLGSLVMESFGPVVVASVVANITMRELPGYRAAYEMPYFPEIGGWEVMLFLVLGVLA 225 Query: 243 GVLAALLTLALYRIEDGFHRLPLHWMWWPALGGIAVGLGGLLQPRALGVGYDVIADLLNQ 302 GVLA L + GF +L L +GG+ VGL + P G GY ++ LL+ Sbjct: 226 GVLAPQFLRVLELGKHGFGKLRLPLPVRLGVGGLLVGLISVQVPEVWGNGYSLVNSLLHT 285 Query: 303 HMLVSAALALLLVKAVIWLLALGSGTSGGVLAPLLMMGAGLGLVLAP----WLPG--GSP 356 + A L +L+VK + + +GSG GG+ P L +GA +G + LP P Sbjct: 286 SWVWQAVLMVLVVKVLATSITVGSGAVGGIFTPTLFVGAAVGYLFGDAAQALLPVHISQP 345 Query: 357 GLWPLVCMAGVLGSVLGAPLTAIVFAFGLTHAGNALLPLIATTAVAYGVNVLWMKRSIMT 416 + +V M L + APL AI+ F +T + +LPL+ + VAY V RS+ Sbjct: 346 FAYAMVGMGAFLAAASYAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVV-----ARSVDG 400 Query: 417 EKIARRGLHVHREYGVDPLERV-----HVEDLMSAPQAV---EAALPAGEALARYYIIER 468 + L HR D ER+ H+ +L+ + V EA+L AL Y ++ Sbjct: 401 RSMYEITLKRHR----DNEERLRLRGTHMSELIRPAETVLHEEASLEEISALFLKYPVKY 456 Query: 469 VHRYYPVTAHGRLLGMVALSRLQ--FADPAEGCGTLMQADAGYLLPHLSARAAAGA---- 522 V Y V R LG+VAL + D E G + + +L PH Sbjct: 457 V---YIVDGRQRYLGVVALQDITSWLLDKKEAAGRVAR---DFLRPHFLHEVTPDMSLGE 510 Query: 523 -----MARQGVARLPVVNGPERRQLVGVLSLSDLARA 554 + QG RLPV+ + QL+GV+ + L A Sbjct: 511 ALQLFLDHQG-ERLPVIASADDPQLLGVVFKTSLLDA 546 >gi|300311782|ref|YP_003775874.1| chloride channel protein [Herbaspirillum seropedicae SmR1] Length = 442 Score = 61.6 bits (148), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 88/295 (29%), Positives = 134/295 (45%), Gaps = 30/295 (10%) Query: 64 WMAALLPVIGGLIVGLMARYGSDKIRGHG---IPEAIEAILFGKSRMSPKVALLKPLSSG 120 W+ LLP +GG VG + + + I E + RM+P V LL ++S Sbjct: 53 WIIWLLP-LGGFAVGWLYLHTGKPVEAGNNLLIDEIHDPRKVIPLRMAPLV-LLGTVASH 110 Query: 121 IVIGSGGPFGAEGPIIMTGGSLGSLLAQWLHLTAAERKTLLVAGACAGMTAIFGTPLAAL 180 + G G EG + GG+L L L L +R+ LL+AG AG +++FGTP+A Sbjct: 111 LF---GASVGREGTAVQMGGALADQLTHLLRLRPEDRRILLMAGISAGFSSVFGTPMAGA 167 Query: 181 LLAVELL-LFELRPRSLLPVALACAIAGFLRPWLIEGGVLFPLEVAPVAPIALLSCLAA- 238 + +E+L + +R ++ P +A A + L G + AP+AL S A Sbjct: 168 IFGLEVLAIGRMRYEAIFPCFVAAIAAD--QVGLAWGVHHTHYAMNASAPMALWSVAAVI 225 Query: 239 ------GLASGVLA-ALLTLALYRIEDGFHRLPLHWMWWPALGGIAVGLG--GLLQPRAL 289 GLA + A + TL+ + ++ PL P +GG V L + R + Sbjct: 226 AAGVVFGLAGMIFANSTHTLSAF-MKRHVSYAPLR----PFIGGFIVALAVWAVGTHRYI 280 Query: 290 GVGYDVIADLLNQHMLVSAALALLLVKAVIWLLALGSGTSGGVLAPLLMMGAGLG 344 G+G VI + Q + A L K V + +LGSG GG + PL +GA LG Sbjct: 281 GLGIPVIVEAFQQPL----APWDFLGKMVFTVTSLGSGFKGGEVTPLFYIGATLG 331 >gi|300310046|ref|YP_003774138.1| chloride-channel protein [Herbaspirillum seropedicae SmR1] Length = 441 Score = 55.8 bits (133), Expect = 9e-09, Method: Compositional matrix adjust. Identities = 112/401 (27%), Positives = 175/401 (43%), Gaps = 38/401 (9%) Query: 49 RLSFEAISPAAHTLGWMAALL-PVIGGLIVGLMARY--GSDKIRGHGIPEAI-----EAI 100 + F H W+ LL P + V L RY GS+ G GIP+AI + Sbjct: 54 EIGFGVFRSIQHRSPWLPLLLTPAVCAACVWLTRRYFPGSE---GSGIPQAIATLHGDTA 110 Query: 101 LFGKSRMSPKVALLKPLSSGIVIGSGGPFGAEGPIIMTGGSLGSLL-----AQWLHLTAA 155 G + +S +V L K + S + I G G EGP + G ++ L A + H+ Sbjct: 111 ERGSTLLSLRVLLGKIVLSVVAIAGGMTIGREGPTVQIGAAIMYNLRRLYPAGYEHM--- 167 Query: 156 ERKTLLVAGACAGMTAIFGTPLAALLLAVELLLFELRPRSLLPVALACAIAGFLR----- 210 ER+ L++AGA AG+ A F TPLA ++ A+E L R+ V AG + Sbjct: 168 ERR-LILAGAAAGLAAAFNTPLAGIVFAIEELSRSFEQRASGVVITTIIFAGLVALSING 226 Query: 211 PWLIEGGVLFPLEVAPVAPIALL-SCLAAGLASGVLAALLTLALYRIEDGFHRLPLHW-M 268 + G + FP E + +A++ + + G+A G+ L+ I L + Sbjct: 227 NYTYFGSIAFPGETSGRLILAVIVTAVLMGVAGGLFCWLILNPTRWIPPALVTLRTERPV 286 Query: 269 WWPALGGIAVGLGGLLQPRA-LGVGYDVIADLLNQHMLVSAALALLLVKAVIWLLALGS- 326 + A+ G V + G+ A G GY+ LLN +S L ++I GS Sbjct: 287 AFAAVCGFIVAVIGVAAGGATFGSGYEQAHGLLNDGQGLSVFYPFLKFASMI-----GSY 341 Query: 327 --GTSGGVLAPLLMMGAGLGLVLAPWLPGGSPGLWPLVCMAGVLGSVLGAPLTAIVFAFG 384 G GG+ AP L +GAG G +L G S + + M G L +V +P+TA V Sbjct: 342 LPGLPGGIFAPSLSIGAGFGNLLHLLFGGTSLPMLVALAMVGYLAAVTQSPITAFVIVME 401 Query: 385 LTHAGNALLPLIATTAVAYGVNVLWMKRSIMTEKIARRGLH 425 + ++ L+AT +A V+ ++ + E +A R L Sbjct: 402 MVDGHALVISLMATALIASAVSKVFAPP--LYETLAERYLK 440 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.140 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,480,434 Number of extensions: 55487 Number of successful extensions: 360 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 351 Number of HSP's successfully gapped: 4 Length of query: 571 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 481 Effective length of database: 1,193,720 Effective search space: 574179320 Effective search space used: 574179320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)