BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0111.p (334 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310915|ref|YP_003775007.1| alkanesulfonates ABC transporte... 529 e-151 gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporte... 288 4e-79 gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate A... 244 6e-66 gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate A... 226 2e-60 gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate A... 95 1e-20 >gi|300310915|ref|YP_003775007.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 336 Score = 529 bits (1362), Expect = e-151, Method: Compositional matrix adjust. Identities = 276/336 (82%), Positives = 299/336 (88%), Gaps = 2/336 (0%) Query: 1 MPQYEDKHQPALAAVELNSKRRRLIGAGLALGAAGAAAAFSPRLAHAAT-RQLRVGYQKG 59 MP + + A+ + +RRRLI + LGAA AA A P HAA RQLRVGYQKG Sbjct: 1 MPHDQAQRNKAIQTAVRSPRRRRLIAGAVTLGAAAAAGASLPAWVHAAAPRQLRVGYQKG 60 Query: 60 ALSIIRAHGTLEQRLASLGATVKWVEFSAGPVQLEALGAGSIDFGDVGEAPPIFSQAAGT 119 ALS+IRAHG LEQRLA LGATVKWVEFSAGPVQLEALGAGSIDFGDVGEAPPIF+QAAGT Sbjct: 61 ALSLIRAHGVLEQRLAPLGATVKWVEFSAGPVQLEALGAGSIDFGDVGEAPPIFAQAAGT 120 Query: 120 PLVYVGATVPRPYVEAILVKDDS-LQKPAQLKGKRIALNKGSNVHYFLVKLLEKHGLQYG 178 PLVYVGATVPRPYVEA+LVKDDS LQ+P QLKGKRIALNKGSNVHYFLVKLLEKHGL+YG Sbjct: 121 PLVYVGATVPRPYVEAVLVKDDSELQRPQQLKGKRIALNKGSNVHYFLVKLLEKHGLKYG 180 Query: 179 DVTPVWLAPADARAAFERGSVDAWAIWEPYLAAAQQTLKARILADADGVVKNRAYYFSTR 238 DVTPVWLAPADARAAFERG+VDAWAIWEPYLAAAQQ LKARILADADGVVKNRAYYFSTR Sbjct: 181 DVTPVWLAPADARAAFERGAVDAWAIWEPYLAAAQQGLKARILADADGVVKNRAYYFSTR 240 Query: 239 DYARDNADLIRIALEELAKVDEFAKTRPDDYASELAGILGLPKGVLDVVVRRYNYGTLPI 298 DYARD+ADL+RIALEEL K+D +A+ RPD+YASELAGILGLP+ VLDVVVRRY+YGTLPI Sbjct: 241 DYARDHADLLRIALEELGKIDAWAQARPDEYASELAGILGLPRSVLDVVVRRYHYGTLPI 300 Query: 299 THAILDEQQQIADTFLDLKLIPKKINLRETAPPWLA 334 T AILDEQQQIADTFLDL+LIP+KI+L E APPWLA Sbjct: 301 TRAILDEQQQIADTFLDLRLIPRKISLLEAAPPWLA 336 >gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 327 Score = 288 bits (737), Expect = 4e-79, Method: Compositional matrix adjust. Identities = 154/316 (48%), Positives = 214/316 (67%), Gaps = 6/316 (1%) Query: 17 LNSKRRRLIG--AGLALGAAGAAAAFSPRLAHAATRQLRVGYQKGA--LSIIRAHGTLEQ 72 ++ RRR I + +ALGA A SP A T+ LR+GYQK A L +++AHGTLE+ Sbjct: 9 VHQARRRAIQRLSAIALGALTIALGASPVQAQE-TKLLRIGYQKAANTLVLLKAHGTLEK 67 Query: 73 RLASLGATVKWVEFSAGPVQLEALGAGSIDFGDVGEAPPIFSQAAGTPLVYVGATVPRPY 132 RL LG VKW EF+AGP LEAL GS+DFG VGEAPP+F+QAAG VY +P P Sbjct: 68 RLQPLGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFVYTAYEIPTPD 127 Query: 133 VEAILVKDDS-LQKPAQLKGKRIALNKGSNVHYFLVKLLEKHGLQYGDVTPVWLAPADAR 191 E +LV+ +S ++ A+LKGK+IA NKGS+VH+ +V LL+K GLQY D+ PV+LAPADAR Sbjct: 128 AEGLLVQKNSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLQYSDIQPVYLAPADAR 187 Query: 192 AAFERGSVDAWAIWEPYLAAAQQTLKARILADADGVVKNRAYYFSTRDYARDNADLIRIA 251 AAFERG+VDAWAIW+P+ AAA++ + AR LA G V + ++ S R +A N + +I Sbjct: 188 AAFERGAVDAWAIWDPFQAAAEKQVGARRLATGVGAVNHHQFFLSARPFAAKNPQVEKIL 247 Query: 252 LEELAKVDEFAKTRPDDYASELAGILGLPKGVLDVVVRRYNYGTLPITHAILDEQQQIAD 311 LEE+++ E+ +T A++LA I GL +++ ++RY + P+ +L EQQ+IAD Sbjct: 248 LEEISREGEWVRTHTAQAAAQLAPIQGLDADIIETGLKRYAHVYKPVDAQVLAEQQKIAD 307 Query: 312 TFLDLKLIPKKINLRE 327 F +LKLIPK + + + Sbjct: 308 AFYELKLIPKPLKVSD 323 >gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 244 bits (624), Expect = 6e-66, Method: Compositional matrix adjust. Identities = 135/316 (42%), Positives = 196/316 (62%), Gaps = 6/316 (1%) Query: 18 NSKRRRLIGAGLALGAAGAAAAFSPRLAHAATRQLRVGYQKGA--LSIIRAHGTLEQRLA 75 +S RRRL+ A ALG G+A A + A + +R+GYQK + L+I++A+GTLE+ LA Sbjct: 7 DSTRRRLLHAATALGL-GSALAPASVFAQG-NKAIRIGYQKSSTLLTILKANGTLEKLLA 64 Query: 76 SLGATVKWVEFSAGPVQLEALGAGSIDFG-DVGEAPPIFSQAAGTPLVYVGATVPRPYVE 134 GA + W EF++G LEAL G +D DV + P+F+QAAG L YV P P + Sbjct: 65 PTGAKISWHEFASGLPLLEALNVGGVDLSADVADTVPVFAQAAGAKLAYVAQESPSPAAQ 124 Query: 135 AILVKDDS-LQKPAQLKGKRIALNKGSNVHYFLVKLLEKHGLQYGDVTPVWLAPADARAA 193 AI+V+ DS L+K + LKGK+IA+ K + VHY L+ LEK GL++ DV +L+PAD RAA Sbjct: 125 AIVVRADSVLRKASDLKGKKIAVTKAAGVHYLLIATLEKAGLKFSDVDAAYLSPADGRAA 184 Query: 194 FERGSVDAWAIWEPYLAAAQQTLKARILADADGVVKNRAYYFSTRDYARDNADLIRIALE 253 FERGSVDAW W+P+LA Q+ + RIL+D GV + YY ++ +A+ +++R+ + Sbjct: 185 FERGSVDAWVTWDPFLAGVQRQSQVRILSDGRGVADYQRYYLASASFAQARPEVLRVVFD 244 Query: 254 ELAKVDEFAKTRPDDYASELAGILGLPKGVLDVVVRRYNYGTLPITHAILDEQQQIADTF 313 EL K + K P + A+ LA + GL +++ R +Y + L EQQ+IADTF Sbjct: 245 ELQKTGHWVKQHPKEAAAFLAPLWGLDADTIELANSRRSYEVRAVRPEALGEQQRIADTF 304 Query: 314 LDLKLIPKKINLRETA 329 L+P+KIN + A Sbjct: 305 FAAGLLPRKINTNDVA 320 >gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 323 Score = 226 bits (576), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 130/329 (39%), Positives = 190/329 (57%), Gaps = 14/329 (4%) Query: 6 DKHQPALAAVELNSKRRRLIGAGLALGAAGAAAAFSPRLAHAATRQLRVGYQK-GALSII 64 D HQP +RRL+ GLA AGA A+ +P + AA + LR+GYQK L+I+ Sbjct: 2 DAHQP--------RHKRRLLLQGLA---AGALASGTPLVRAAADKVLRIGYQKFNTLNIL 50 Query: 65 RAHGTLEQRLASLGATVKWVEFSAGPVQLEALGAGSIDFGDVGEAPPIFSQAAGTPLVYV 124 + G LEQ L G +VKW EF+AGP EAL A +ID G + P +F+ A+G VY+ Sbjct: 51 KGSGKLEQALQPQGWSVKWFEFAAGPQLTEALNANAIDLGHAADTPSVFANASGVNAVYL 110 Query: 125 GATVPRPYVEAILVKDDS-LQKPAQLKGKRIALNKGSNVHYFLVKLLEKHGLQYGDVTPV 183 GA P P I V +S ++ A LKGK++A +G NV + L+K LE+ GL Y D+ PV Sbjct: 111 GAEQPYPKGIGIFVAQESPIRSVADLKGKKVATGRGWNVQFLLLKALEEVGLSYRDIEPV 170 Query: 184 WLA-PADARAAFERGSVDAWAIWEPYLAAAQQTLKARILADADGVVKNRAYYFSTRDYAR 242 ++ AD RAAF+ G+VDA +W+P+LA + + RIL D G+ NR ++ + + Sbjct: 171 YVTNAADVRAAFQSGNVDAATLWDPFLAGQELATRPRILRDGTGLSNNRTFHLAVPSFVD 230 Query: 243 DNADLIRIALEELAKVDEFAKTRPDDYASELAGILGLPKGVLDVVVRRYNYGTLPITHAI 302 + D++R EL K +++ ++ P + A LA LG+ VL + R NY T+ + I Sbjct: 231 RHKDIVRTVFAELKKTNQWTQSHPQETAELLAPQLGVDPKVLRLATERRNYTTVAVDAGI 290 Query: 303 LDEQQQIADTFLDLKLIPKKINLRETAPP 331 + EQQQIAD FL L LI + +++ P Sbjct: 291 VAEQQQIADAFLKLGLIKNPVRVQDKVYP 319 >gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 94.7 bits (234), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 86/310 (27%), Positives = 145/310 (46%), Gaps = 9/310 (2%) Query: 23 RLIGAGLALGAAGAAAAFSPRLAHAATRQLRVGYQKGALSIIRAHGTLEQRLASLGATVK 82 RL A L + A G S +L Y ++R G LE+ L + G VK Sbjct: 8 RLAPAALGVTALGGIPLLSSAAQPPKVIRLDYAYYSPPSLVLRKFGWLEEDLKAQGTEVK 67 Query: 83 WVEFSAGPVQLEALGAGSIDFGDVGEAPPIFSQAAGTPL--VYVGATVPRPYVEAILV-K 139 WV LE L + S+DFG + ++A G P+ VY+ + RP A+ V K Sbjct: 68 WVLSQGSNRALEYLNSDSVDFGSTAGLAALLARANGNPVKAVYIYS---RPEWTALAVGK 124 Query: 140 DDSLQKPAQLKGKRIALNKGSNVHYFLVKLLEKHGLQYGDVTPVWLAPADARAAFERGSV 199 + ++ +LKGK+IA KG++ + FL++ L ++GL+ DV V L AD RAA E+G V Sbjct: 125 ESPVKSINELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQHADGRAALEQGRV 184 Query: 200 DAWAIWEPYLAAAQQTLKARILADADGVVKNRAYYFSTRD-YARDNADLIRIALEELAKV 258 AWA +P+LAA++ +R++ V N + + D +A + D ++ + + Sbjct: 185 AAWAGLDPHLAASELEAGSRLI--YRNVNFNTYGFLNVSDSFAARHPDQVKRVIAAYERA 242 Query: 259 DEFAKTRPDDYASELAGILGLPKGVLDVVVRRYNYGTLPITHAILDEQQQIADTFLDLKL 318 + PD+ + L+ L V + ++R ++ I ++ + A L+ L Sbjct: 243 RSWIIAHPDETVALLSEESKLSAPVARLQLKRNDFSQPQIGREHIEALRAAAPILLEEDL 302 Query: 319 IPKKINLRET 328 + +L T Sbjct: 303 VKSGTDLPRT 312 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,067,969 Number of extensions: 45668 Number of successful extensions: 194 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 185 Number of HSP's successfully gapped: 7 Length of query: 334 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 248 Effective length of database: 1,212,660 Effective search space: 300739680 Effective search space used: 300739680 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)