BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0110.p (515 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310916|ref|YP_003775008.1| phospholipase C protein [Herbas... 1014 0.0 gi|300311919|ref|YP_003776011.1| non-hemolytic phospholipase C (... 87 3e-18 >gi|300310916|ref|YP_003775008.1| phospholipase C protein [Herbaspirillum seropedicae SmR1] Length = 558 Score = 1014 bits (2623), Expect = 0.0, Method: Compositional matrix adjust. Identities = 486/516 (94%), Positives = 504/516 (97%), Gaps = 2/516 (0%) Query: 1 VPYPSGNPAESAALDRALRDNVKTIVVIYAENRSFNNLFADFPGLEKPLSSLKPSDYQQR 60 P PSGNPAE+AALDRALR+ VKTIVVIYAENRSFNNLFADFPGLEKPLS+LKPSDYQQR Sbjct: 42 APPPSGNPAEAAALDRALREQVKTIVVIYAENRSFNNLFADFPGLEKPLSALKPSDYQQR 101 Query: 61 DRDGSLLKELPPAWNGVLIQDQSVEGVNYAGGKQYQTNLPNAPYALKGPQGEALPLSLVT 120 DRDGSLLK+LPPAW+GV+ Q+Q+VEGV Y GKQYQTNLPNAPYALKGPQGEALPL LVT Sbjct: 102 DRDGSLLKQLPPAWSGVVNQEQTVEGVTYEAGKQYQTNLPNAPYALKGPQGEALPLGLVT 161 Query: 121 RDLWHVFYQNQMQINGGKNDGFVAWADSGGLTMGHYADTRYNLRLWDVAREFVLCDNFFQ 180 RDLWHVFYQNQMQINGGKNDGFVAWADSGGLTMGHYADTRY+LRLWDVAREFVLCDNFFQ Sbjct: 162 RDLWHVFYQNQMQINGGKNDGFVAWADSGGLTMGHYADTRYHLRLWDVAREFVLCDNFFQ 221 Query: 181 GAFGGSFLNHQYLICATAPYYPNVMSSVSRTQVAKTKNNDPLSPELVPAEGSPASALQGP 240 GAFGGSFLNHQYLICATAPYYPNVM+SVSRTQVAKTKNNDPLSPELVPAEGSP SALQGP Sbjct: 222 GAFGGSFLNHQYLICATAPYYPNVMNSVSRTQVAKTKNNDPLSPELVPAEGSPTSALQGP 281 Query: 241 PKFGPSALTPD--AVNTMAPPYWPTWSRDRNNPAYSEPNQPSVLVPQTHEHIGDKLNKKG 298 PKFGPSALTPD AVNTMAPPYWPTWSRDRNNPAY+EPNQPSVLVPQTHEHIGDKLNKKG Sbjct: 282 PKFGPSALTPDGYAVNTMAPPYWPTWSRDRNNPAYAEPNQPSVLVPQTHEHIGDKLNKKG 341 Query: 299 IDWAWYAGAWQATLDEFKDSKGIPKIPNFQYHHQPFNYFKNLGPENPAERNKRLRDGGLG 358 IDWAWYAGAWQATLDEFKDSKGIPKIPNFQYHHQPFNYFKNLGPENPAERNKRLRDGGLG Sbjct: 342 IDWAWYAGAWQATLDEFKDSKGIPKIPNFQYHHQPFNYFKNLGPENPAERNKRLRDGGLG 401 Query: 359 DESRTNRFLADVEAGRLPPVTFYKPQGNLNLHAGYADIASGDRHIAHIVKSLQASPQWKN 418 DE+RTNRFLADVEAGRLPPVTFYKPQGNLNLHAGYADIASGDRHIAHI+KSLQASPQWKN Sbjct: 402 DEARTNRFLADVEAGRLPPVTFYKPQGNLNLHAGYADIASGDRHIAHIIKSLQASPQWKN 461 Query: 419 MVVVITFDENGGWWDHVAPPKGDRWGPGTRVPALVISPFARKGTVDHTVYDTASILRLIT 478 MVVV+TFDENGGWWDHVAPPKGDRWGPGTRVPALV+SPFARKGTVDHTVYDTASILRLIT Sbjct: 462 MVVVVTFDENGGWWDHVAPPKGDRWGPGTRVPALVVSPFARKGTVDHTVYDTASILRLIT 521 Query: 479 RTFDLEKLNGLKERDDAMIARGQQPMGDLTNALQFK 514 RTFDLEKL+GLK+RD+AMIARGQQPMGDLTNALQ K Sbjct: 522 RTFDLEKLDGLKQRDEAMIARGQQPMGDLTNALQLK 557 >gi|300311919|ref|YP_003776011.1| non-hemolytic phospholipase C (phosphatidylcholine choline phospho hydrolase) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 696 Score = 87.0 bits (214), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 125/531 (23%), Positives = 196/531 (36%), Gaps = 155/531 (29%) Query: 21 NVKTIVVIYAENRSFNNLFADFPGLE-------KPLSSLKPSDYQQRDRDGSLLKELPPA 73 +VK +V++ ENRSF++ F G+ +PL P + QRD G L+ LP Sbjct: 49 DVKHVVILMQENRSFDHYFGCLRGVRGFADPIPRPLPDGSPV-FHQRDGQGRLV--LP-- 103 Query: 74 WNGVLIQDQSVEGVNYAGGKQYQTNLPNAPYALKGPQGEALPLSLVTRDLWHVFYQNQMQ 133 + + AGG NLP H + Sbjct: 104 ----YRMEMATTSAGRAGG-----NLP------------------------HAWKDQHAA 130 Query: 134 INGGKNDGFVAWADSGGLTMGHYADTRYNLRL-WDVAREFVLCDNFFQGAFGGSFLNHQY 192 N G D ++ A +TM ++ TR +L + +A F LCD + H Sbjct: 131 WNRGDYDKWIE-AKKDTVTMAYF--TREDLPFHYALADAFTLCDAY-----------HCS 176 Query: 193 LICATAPYYPNVMSSVSRTQVAKTKNNDPLSPELVPAEGSPASALQGPPKFGPSALTPDA 252 ++ +T P ++MS Q + P G P G FG Sbjct: 177 VLGSTNPNRFHLMSGGIDLQ-GRAGGPMTFQPRTGSCSGVPGERRSGADSFG-------- 227 Query: 253 VNTMAPPYWPTWSRDRNNPAYSEPNQPSVLVPQTHEHIGDKLNKKGIDWAWYAGAWQATL 312 W T+ ++L + G+ W Y Q T Sbjct: 228 --------WTTYP--------------------------ERLEQHGVSWKVY----QGTE 249 Query: 313 DEFKDSKGIPKIPNFQYHHQPFNYFKNLGPENPAERNKRLRDGGLGDESRTNRFLADVEA 372 + KD P +F F ++N P++P + K L D +R DV + Sbjct: 250 ELHKDHGDYPS--DFNVLQYFFAQYQNAAPDSPLWQ-KACSKFTLADLAR------DVNS 300 Query: 373 GRLPPVTFYKPQGNLNLHAGYADIASGDRHIAHIVKSLQASPQ-WKNMVVVITFDENGGW 431 G+LP V++ P L+ G +I+ ++ L A+P W + V+++ +DEN G+ Sbjct: 301 GKLPQVSWLMPP-KLSCEHPDRTPGYGAHYISQVLDILTANPALWSSTVLLVNYDENDGF 359 Query: 432 WDHVAPP---------------------KGDRWGP--------GTRVPALVISPFARKGT 462 +DHV PP KGD P G RVP +VISP+++ G Sbjct: 360 FDHVVPPTPPLHAGQGLSTVETSAEFHRKGDYVNPADDLPAGLGARVPMMVISPWSKGGY 419 Query: 463 VDHTVYDTASILRLITRTFDLEKLNGLKERDDAMIARGQQPMGDLTNALQF 513 V V+D S++R + + FD+ + N R + GDLT+A F Sbjct: 420 VCSEVFDHTSVIRFLEQVFDVHEPNISPWR--------RAVCGDLTSAFDF 462 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.135 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,828,447 Number of extensions: 82762 Number of successful extensions: 207 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 205 Number of HSP's successfully gapped: 2 Length of query: 515 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 426 Effective length of database: 1,198,455 Effective search space: 510541830 Effective search space used: 510541830 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)