BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0095.p (325 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspi... 593 e-171 gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspi... 170 1e-43 gi|300309398|ref|YP_003773490.1| threonine dehydratase [Herbaspi... 169 2e-43 gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspi... 114 1e-26 >gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 593 bits (1528), Expect = e-171, Method: Compositional matrix adjust. Identities = 304/325 (93%), Positives = 317/325 (97%) Query: 1 MIPVSSATESPSFADVLLAAERIKPWAHRTPVLRSRTADVEVGAQLFFKCENFQRMGAFK 60 MIPVSSATE PSFADVLLAAERIKPWAHRTPVLRSRTAD E+GAQLFFKCENFQRMGAFK Sbjct: 1 MIPVSSATELPSFADVLLAAERIKPWAHRTPVLRSRTADTELGAQLFFKCENFQRMGAFK 60 Query: 61 FRGAMNALSQFDSEQRKHGVITFSSGNHAQGTALAAQLLGIPAVIVMPEDAPAAKVAATR 120 FRGAMNAL+QFD+ QRKHGVITFSSGNHAQGTALAAQLLGIPAVIVMP+DAPAAKVAATR Sbjct: 61 FRGAMNALTQFDATQRKHGVITFSSGNHAQGTALAAQLLGIPAVIVMPQDAPAAKVAATR 120 Query: 121 GYGAEVVMYDRYTEDREAIGARLAAERKMTLIPPYDHPHVMAGQGTATLELLQETGPLDA 180 GYGAEVV YDRYTEDREAIGARLAAERKMTLIPPYDHPHVMAGQGTATLELLQETGPLDA Sbjct: 121 GYGAEVVTYDRYTEDREAIGARLAAERKMTLIPPYDHPHVMAGQGTATLELLQETGPLDA 180 Query: 181 LFVCLGGGGLLSGAALAARALSPDCKIYGVEPEAGNDGQQSLRAGQIVHIDTPKTIADGA 240 LFVCLGGGGLLSGAALAARALSP CKIYGVEP+AGNDGQQSLRAGQ+VHIDTPKTIADGA Sbjct: 181 LFVCLGGGGLLSGAALAARALSPACKIYGVEPQAGNDGQQSLRAGQVVHIDTPKTIADGA 240 Query: 241 QTQHLGKYTFDVIRQLVDDIHTVSDAELVSGLRFFAERMKMVVEPTGCLGFAAARKLKDQ 300 QTQHLGKYTF VIR+LVDDIHTVSD ELV+G+RFFAERMKMVVEPTGCLGFAAARKLKDQ Sbjct: 241 QTQHLGKYTFGVIRELVDDIHTVSDEELVAGMRFFAERMKMVVEPTGCLGFAAARKLKDQ 300 Query: 301 LQGKRVGVIISGGNIDLGKLSGYLS 325 LQGKRVGVIISGGN+DLGKL+ +L+ Sbjct: 301 LQGKRVGVIISGGNVDLGKLATFLA 325 >gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 508 Score = 170 bits (431), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 108/308 (35%), Positives = 174/308 (56%), Gaps = 10/308 (3%) Query: 22 RIKPWAHRTPVLRSRTADVEVGAQLFFKCENFQRMGAFKFRGAMNALSQFDSEQRKHGVI 81 R+ A TP+ + T + +++FK E+ Q + +FK RGA N ++ Q K GVI Sbjct: 13 RVYDVAQETPLELANTLSQRIDNRIYFKREDMQSVFSFKLRGAYNKMAHLTPAQLKRGVI 72 Query: 82 TFSSGNHAQGTALAAQLLGIPAVIVMPEDAPAAKVAATRGYGAEVVMY-DRYTEDREAIG 140 S+GNHAQG AL+A LG AVIVMP P K+ A + G EVV++ D +T+ E Sbjct: 73 CASAGNHAQGVALSAARLGCRAVIVMPTTTPQVKIDAVKARGGEVVLFGDSFTDAYEH-A 131 Query: 141 ARLAAERKMTLIPPYDHPHVMAGQGTATLELL-QETGPLDALFVCLGGGGLLSGAALAAR 199 L ++K+T + P+D P+V+AGQGT +E+L Q P+ A+FV +GGGGL++G A + Sbjct: 132 LTLEKKQKLTFVHPFDDPYVIAGQGTVGMEILRQHPDPIHAIFVAIGGGGLIAGVASYVK 191 Query: 200 ALSPDCKIYGVEPEAGNDGQQSLRAGQIVHIDTPKTIADGAQTQHLGKYTFDVIRQLVDD 259 A+ PD KI GV+ + +SL+AG+ V + +DG + +G+ TF + ++LVD+ Sbjct: 192 AVRPDIKIIGVQTTDSDAMARSLKAGRRVALPDVGLFSDGTAVKLVGEETFRIAKELVDE 251 Query: 260 IHTVSDAELVSGLRFFAERMKMVVEPTGCLGFAAARKL-------KDQLQGKRVGVIISG 312 + V + + ++ + + +VEP G L A A+ K ++G+ + I G Sbjct: 252 VIIVDTDAVCTAIKDIFQDTRSIVEPAGALAVAGAKAYVERAKASKKPIKGESLITIACG 311 Query: 313 GNIDLGKL 320 N++ +L Sbjct: 312 ANMNFDRL 319 >gi|300309398|ref|YP_003773490.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 401 Score = 169 bits (429), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 119/306 (38%), Positives = 168/306 (54%), Gaps = 3/306 (0%) Query: 12 SFADVLLAAERIKPWAHRTPVLRSRTADVEVGAQLFFKCENFQRMGAFKFRGAMNALSQF 71 + D+ AA R++ TP + SRT VGAQ+F K EN Q +FK RGA N L Sbjct: 3 TLNDIEAAARRLQGQILETPCVESRTLSQIVGAQVFLKFENLQFTASFKERGACNKLVDV 62 Query: 72 D-SEQRKHGVITFSSGNHAQGTALAAQLLGIPAVIVMPEDAPAAKVAATRGYGAEVVMYD 130 S GV+ S+GNHAQG A AQ LG+PA IVMP P K+ TRG+GA VV++ Sbjct: 63 AASGGGARGVVAMSAGNHAQGVAYHAQRLGLPATIVMPRFTPGVKIERTRGFGANVVLHG 122 Query: 131 RYTEDREAIGARLAAERKMTLIPPYDHPHVMAGQGTATLELLQETGPLDALFVCLGGGGL 190 E LA + +T + PYD ++AGQGT LE+++ LD L V +GGGGL Sbjct: 123 DTLEAARTHAYELAEKEGLTFVHPYDDEAIIAGQGTVALEMMKAVPDLDCLVVSIGGGGL 182 Query: 191 LSGAALAARALSPDCKIYGVEPEAGNDGQQSLRAGQIVHIDTPKTIADGAQTQHLGKYTF 250 L+G A+AARA+ P+ +I GV+ +++ + H TIA+G G T Sbjct: 183 LAGMAVAARAIKPEIEIVGVQTRRFPTMVNAVKGTE--HPVGASTIAEGIAVGTPGTLTR 240 Query: 251 DVIRQLVDDIHTVSDAELVSGLRFFAERMKMVVEPTGCLGFAAARKLKDQLQGKRVGVII 310 +I + D++ V + ++ + E K +VE G G AA + D+ +GKRVG+++ Sbjct: 241 AIIAREADELLLVDEGDIEQAVLMLLEIEKTLVEGAGAAGLAALVRYPDRFRGKRVGLVL 300 Query: 311 SGGNID 316 SGGNID Sbjct: 301 SGGNID 306 >gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 351 Score = 114 bits (285), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 96/308 (31%), Positives = 148/308 (48%), Gaps = 4/308 (1%) Query: 11 PSFADVLLAAERIKPWAHRTPVLRSRTADVEVGAQLFFKCENFQRMGAFKFRGAMNALSQ 70 PS D+ AA + T L + +GA+++ K EN GAFK RG M L Sbjct: 10 PSLTDIEQAAGIVYAGMAPTAQLCWPLLNQALGAEVWVKHENHAPTGAFKVRGGMVYLHH 69 Query: 71 FDSEQRK-HGVITFSSGNHAQGTALAAQLLGIPAVIVMPEDAPAAKVAATRGYGAEVVMY 129 +Q + GV++ + GNH Q LAA+ LGI A IV+P+ K AA R G E++ + Sbjct: 70 LARQQPQLSGVVSATRGNHGQSVGLAARRLGISATIVVPQGNSREKNAAMRALGVELIEH 129 Query: 130 -DRYTEDREAIGARLAAERKMTLIPPYDHPHVMAGQGTATLELLQETGPLDALFVCLGGG 188 + E RE A ++ + P H ++AG + +EL LD + V +G G Sbjct: 130 GSEFQESREYAQQLAAQQQLHMI--PSLHRDLVAGVASYWMELFAAQPGLDVVLVPIGQG 187 Query: 189 GLLSGAALAARALSPDCKIYGVEPEAGNDGQQSLRAGQIVHIDTPKTIADGAQTQHLGKY 248 + GA A AL +I GV + S AG+ + IADG + + Sbjct: 188 SGMCGAVAARNALGLSTRIIGVVSAHALAYKLSFEAGRKIESPVSTCIADGVACRVPDQA 247 Query: 249 TFDVIRQLVDDIHTVSDAELVSGLRFFAERMKMVVEPTGCLGFAAARKLKDQLQGKRVGV 308 + +V+ VD++ V+D E++ ++ V E G AAA +L+ +L+G +VGV Sbjct: 248 SLEVLFSEVDEVVAVTDDEVMDAMKLAYIATHNVAEGAGACALAAAWQLRARLRGLKVGV 307 Query: 309 IISGGNID 316 +SGGN+D Sbjct: 308 TLSGGNVD 315 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 927,215 Number of extensions: 35086 Number of successful extensions: 122 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 117 Number of HSP's successfully gapped: 4 Length of query: 325 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 240 Effective length of database: 1,217,395 Effective search space: 292174800 Effective search space used: 292174800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)