BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0093.p (546 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310928|ref|YP_003775020.1| medium-chain-fatty-acid CoA lig... 1016 0.0 gi|300313986|ref|YP_003778078.1| acyl-CoA synthetase/AMP-acid li... 237 1e-63 gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligas... 162 5e-41 gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligas... 136 3e-33 gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [H... 120 3e-28 >gi|300310928|ref|YP_003775020.1| medium-chain-fatty-acid CoA ligase [Herbaspirillum seropedicae SmR1] Length = 545 Score = 1016 bits (2628), Expect = 0.0, Method: Compositional matrix adjust. Identities = 498/545 (91%), Positives = 517/545 (94%) Query: 1 MPQTIEQTPSAYGYPLLIKQLLHSALATAPEQEIIYGARRLTYAGFYERVQRLANVLKEM 60 MPQ I QTPSAYGYPLLIKQLLHSALATA QEIIYG RRL+YAGFYERVQRLAN LKEM Sbjct: 1 MPQAIHQTPSAYGYPLLIKQLLHSALATASGQEIIYGQRRLSYAGFYERVQRLANALKEM 60 Query: 61 GVQAGNTVAVMDWDSNRYLECFFAVPMMGCVLQTVNVRLSPEQIAYTLNHAQADVLLVNS 120 G+Q GNTVAVMDWDSNRYLECFFAVPMMGCVLQTVNVRLSPEQIAYTLNHAQADVLLVNS Sbjct: 61 GLQPGNTVAVMDWDSNRYLECFFAVPMMGCVLQTVNVRLSPEQIAYTLNHAQADVLLVNS 120 Query: 121 DFMPVLKQIRGELNTLTRCVLLTDDGSPCPQGPGFAGEYEALLAQASAQYDFPDFDENAR 180 DF+P+LKQIRGEL TLTRCV LTDD +PCP+GPGFAGEYE LLAQA DFPDFDENAR Sbjct: 121 DFLPLLKQIRGELTTLTRCVWLTDDATPCPRGPGFAGEYEELLAQAEPACDFPDFDENAR 180 Query: 181 ATTFYTTGTTGLPKGVYFSHRQLVLHTLGTTAGLALAPQQGRLHRDDVYMPLTPMFHVHA 240 ATTFYTTGTTGLPKGVYFSHRQLVLHTL TTAGLALAPQQGR HRDDVYMPLTPMFHVHA Sbjct: 181 ATTFYTTGTTGLPKGVYFSHRQLVLHTLATTAGLALAPQQGRFHRDDVYMPLTPMFHVHA 240 Query: 241 WGLPYVATMAGVKQVYPGRYVPDTICKLIREEKVTFSHCVPTILQMVLTSEQAREVDLSG 300 WGLPYVATMAGVKQVYPGRYVPDTIC+LIREEKVTFSHCVPTILQMVLTSEQARE DLSG Sbjct: 241 WGLPYVATMAGVKQVYPGRYVPDTICRLIREEKVTFSHCVPTILQMVLTSEQAREADLSG 300 Query: 301 WKMIIGGSAMTGSLARMARDRGIDVFTGYGMSETCPILTLAQVPTEDLGSEREAVLRVKT 360 WKMIIGGSAMTGSLA+MARDRGIDVFTGYGMSETCP+LTLAQVPTE+LG EREA +RVKT Sbjct: 301 WKMIIGGSAMTGSLAKMARDRGIDVFTGYGMSETCPVLTLAQVPTEELGGEREAAIRVKT 360 Query: 361 GRPVPLVALRTVDGDMNDCPRDGKSTGEVVVRAPWLTQGYLHQAEASEELWRGGWLHTQD 420 GRP+PLV LRTVDG+M DCPRDG +TGEVVVRAPWLTQGYLHQAEASEELWRGGWLHTQD Sbjct: 361 GRPLPLVDLRTVDGEMQDCPRDGSTTGEVVVRAPWLTQGYLHQAEASEELWRGGWLHTQD 420 Query: 421 IGHLDAEGYLQVTDRIKDVIKTGGEWVSSLQIEDIIARHPAVAETAVIGVQDGKWGERPV 480 IGHLDAEGYLQVTDRIKDVIKTGGEWVSSLQIEDIIARHPAVAETAVIG+QDGKWGERPV Sbjct: 421 IGHLDAEGYLQVTDRIKDVIKTGGEWVSSLQIEDIIARHPAVAETAVIGIQDGKWGERPV 480 Query: 481 ALVVLKKDVDAVSEEDIRQHVMAAAEAGTISRYGVPDRVRFVPELARTSVGKLNKRVMRE 540 ALVVLKKD AVSE+DIR+HV AAAEAG ISRYGVPDRVRFV ELARTSVGKLNKRVMRE Sbjct: 481 ALVVLKKDAAAVSEDDIREHVRAAAEAGAISRYGVPDRVRFVAELARTSVGKLNKRVMRE 540 Query: 541 QYPAA 545 QYP++ Sbjct: 541 QYPSS 545 >gi|300313986|ref|YP_003778078.1| acyl-CoA synthetase/AMP-acid ligase II protein [Herbaspirillum seropedicae SmR1] Length = 546 Score = 237 bits (605), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 174/521 (33%), Positives = 265/521 (50%), Gaps = 33/521 (6%) Query: 34 IIYGARRLTYAGFYERVQRLANVLKEMGVQAGNTVAVMDWDSNRYLECFFAVPMMGCVLQ 93 II+GA R + Y R +RLA+ L+ +GV +TV+ M ++ +E F VPM G VL Sbjct: 37 IIHGALRQDWDQTYRRTRRLASALQGLGVGKNDTVSAMLPNTPAMVEAHFGVPMAGAVLN 96 Query: 94 TVNVRLSPEQIAYTLNHAQADVLLVNSDFMPVLKQIRGELNTLTRCVLLTDDGSPCPQGP 153 +N+RL E I + L H +A VLL++S+F + +Q+R +L L + + G P G Sbjct: 97 ALNIRLDAESIVFMLRHGEAKVLLIDSEFAALAQQLRTQLPALKIVEVFDELGPPPVAGE 156 Query: 154 GFAG-EYEALLAQASAQYDF--PDFDENARATTFYTTGTTGLPKGVYFSHRQLVLHTLGT 210 F EYEALLA +D+ P + +A A YT+GTTG PKGV + HR L+ + Sbjct: 157 RFGHLEYEALLAGGDEHFDWQMPADEWDAIALN-YTSGTTGDPKGVVYHHRGAALNAVSN 215 Query: 211 TAGLALAPQQGRLHRDDVYMPLTPMFHVHAWGLPY-VATMAGVKQVYPGRYVPDTICKLI 269 + L + VY+ PMFH + W P+ VA AGV V ++ P + LI Sbjct: 216 IL-------EWDLPKHPVYLWTLPMFHCNGWCFPWTVAARAGV-NVCLRKFEPKLVFDLI 267 Query: 270 REEKVTFSHCVPTILQMVLTSEQAREVDLSG--WKMIIGGSAMTGSLARMARDRGIDVFT 327 E +T P + + + + + G M+ G LA+M + ++ Sbjct: 268 AEHGITHYCAAPIVHAALANAPEGWRAGIRGPVKAMVAGAPPPAAVLAKMEAMQ-FELTH 326 Query: 328 GYGMSETC-PILTLA-QVPTEDLGSEREAVLRVKTGRPVPLVALRTV--DGDMNDCPRDG 383 YG++E P A Q L ++ AV + + G L + TV M DG Sbjct: 327 VYGLTEVYGPAAVCAEQDEWNTLSVDQRAVQKSRQGVRYHLQSGVTVLSPDTMAPVAADG 386 Query: 384 KSTGEVVVRAPWLTQGYLHQAEASEELWRGGWLHTQDIGHLDAEGYLQVTDRIKDVIKTG 443 + GE++ R +GYL A+ E + GGW HT D+G ++A+GY+++ DR KD+I +G Sbjct: 387 EEIGEIMFRGNICMKGYLKNDRATHEAFAGGWFHTGDLGVMNADGYIKIKDRSKDIIISG 446 Query: 444 GEWVSSLQIEDIIARHPAVAETAVIGVQDGKWGERPVALVVLKKDVD---AVSEEDIRQH 500 GE +SS+++ED++ RHPAV AV+ D KWGE P A V LK+ + A E R H Sbjct: 447 GENISSVEVEDVLYRHPAVLAAAVVAQPDEKWGETPCAFVELKEGAEVGAAELTEFCRSH 506 Query: 501 VMAAAEAGTISRYGVPDRVRFVPELARTSVGKLNKRVMREQ 541 ++ + VP + F P L +TS GK+ K +R++ Sbjct: 507 ---------LAGFKVPKAIYFGP-LPKTSTGKIQKFELRKR 537 >gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 162 bits (411), Expect = 5e-41, Method: Compositional matrix adjust. Identities = 140/500 (28%), Positives = 224/500 (44%), Gaps = 34/500 (6%) Query: 49 RVQRLANVLKEMGVQAGNTVAVMDWDSNRYLECFFAVPMMGCVLQTVNVRLSPEQIAYTL 108 RV LA+ L GV+ G+ + V ++ + E +A MG V N R++P + AY Sbjct: 71 RVDALAHALTRRGVKKGDKMLVHSRNNQQIFESAWAAFKMGVVWVPTNFRITPPEAAYLG 130 Query: 109 NHAQADVLLVNSDFMPVLKQIRGELNTLTRCVLLTDDGSPCPQGPGFAGE--YEALLAQA 166 + A V+L + F + ++ L V L D AGE YEAL+ + Sbjct: 131 QSSGACVMLYDRGFAQYVDAVKAASPALEHVVALAD---------ARAGELDYEALVREG 181 Query: 167 S-AQYDFPDFDENARATTFYTTGTTGLPKGVYFSHRQLVLHTLGTTAGLALAPQQGRLHR 225 S A + + D + FYT+GTTG PK SH Q+ A L P R Sbjct: 182 SGAAFREAEVDYDDPLWFFYTSGTTGHPKAGMLSHGQMAFVVTNHLADLM--PGLTHQSR 239 Query: 226 DDVYMPLTPMFHVHAWGLPYVATMAGVKQVY--PGRYVPDTICKLIREEKVTFSHCVPTI 283 V PL+ VHA + T G + R V + +L+ + +V VPTI Sbjct: 240 SLVVAPLSHGAGVHA----VINTARGAASILLSSERLVVEEAWQLVEKYRVDNLFTVPTI 295 Query: 284 LQMVLTSEQAREVDLSGWKMII--GGSAMTGSLARMARDRGIDVFTGYGMSETCPILTLA 341 +++++ E D S K +I G + G + YG+ E +T Sbjct: 296 VKILVEDESVDRYDHSSLKHVIYAGAPMYRADQVYALKKLGKVLVQYYGLGEVTGNITFL 355 Query: 342 QVPTEDLGSEREAVLRVKT-GRPVPLVALRTVDGDMND-CPRDGKSTGEVVVRAPWLTQG 399 P + RV T G P + + ++ D + P + TGE+ VR P + G Sbjct: 356 P-PYLHFEDDAHPQARVGTCGMPRTGMQIAILNDDGQELAPFE---TGEICVRGPAVFMG 411 Query: 400 YLHQAEASEELWRGGWLHTQDIGHLDAEGYLQVTDRIKDVIKTGGEWVSSLQIEDIIARH 459 Y + EA+ + ++ W HT D+GH+D +G+L +T R D+ +GG V +IE+ + H Sbjct: 412 YHNNPEANAKAFKHDWFHTGDLGHVDQDGFLYITGRSSDMYISGGSNVYPREIEEALLTH 471 Query: 460 PAVAETAVIGVQDGKWGERPVALVVLKKDVDAVSEEDIRQHVMAAAEAGTISRYGVPDRV 519 PAV+E AV+GV D KWGE +A++V K + + + + A I +Y P R Sbjct: 472 PAVSEVAVLGVPDPKWGESGLAVIVCK------AGQQVDGEALLAHLESRIGKYKWPRRF 525 Query: 520 RFVPELARTSVGKLNKRVMR 539 + + ++ GK+ K+ ++ Sbjct: 526 IYWDSMPKSGYGKIVKKQIK 545 >gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 566 Score = 136 bits (343), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 106/366 (28%), Positives = 174/366 (47%), Gaps = 30/366 (8%) Query: 185 YTTGTTGLPKGVYFSHRQLVLHTLGTTAGLALAPQQGRLHRDDVYMPLTPMFHVHAWGLP 244 YT GTTG+ KG +HR LV + L L A + G+ V++ P++H+ A Sbjct: 212 YTGGTTGVAKGAVLTHRNLVANVLQAEEWLTPALKAGKPIDQLVFVCALPLYHIFALT-- 269 Query: 245 YVATMAGVKQ-----VYPG-RYVPDTICKLIREEKVTFSHCVPTILQMVLTSEQAREVDL 298 V M G ++ + P R +P + +L + + TF V T+ +L + +D Sbjct: 270 -VCNMLGTREGALNLLIPNPRDIPGFVKELAKYKVNTFP-AVNTLYNALLNNADFARLDF 327 Query: 299 SGWKMIIGGS-AMTGSLA-RMARDRGIDVFTGYGMSETCPILTLAQVPTEDLGSEREAVL 356 SG++ +GG A+ S+A + + G + GYG+SET PI + ++ Sbjct: 328 SGYRCCVGGGMAVQKSVADKWKKLTGCPIIEGYGLSETSPIASANPCTIDEYTG------ 381 Query: 357 RVKTGRPVPLVALRTVDGDMNDCPRDGKSTGEVVVRAPWLTQGYLHQA-EASEELWRGGW 415 G P+P +D D P GE+ +R P + GY + E ++ + G+ Sbjct: 382 --TIGLPLPSTEFAILDDDGQPVPLG--QPGEIAIRGPQVMPGYWQRPDETAKVMTPDGF 437 Query: 416 LHTQDIGHLDAEGYLQVTDRIKDVIKTGGEWVSSLQIEDIIARHPAVAETAVIGVQDGKW 475 T DIG +D GY ++ DR KD+I G V ++E ++A+HP V E A +GV D Sbjct: 438 FKTGDIGVMDERGYTKIVDRKKDMILVSGFNVYPNEVEGVVAQHPGVLECACVGVPDEHT 497 Query: 476 GERPVALVVLKKDVDAVSEEDIRQHVMAAAEAGTISRYGVPDRVRFVPELARTSVGKLNK 535 GE V L V++KD +S + + + Y P + F EL +T+VGK+ + Sbjct: 498 GEA-VKLFVVRKD-KTLSAAQLMDYCKE-----QFTGYKKPKYIEFRDELPKTNVGKILR 550 Query: 536 RVMREQ 541 R +R++ Sbjct: 551 RELRDE 556 >gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 549 Score = 120 bits (300), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 129/502 (25%), Positives = 207/502 (41%), Gaps = 42/502 (8%) Query: 52 RLANVLKEMGVQAGNTVAVMDWDSNRYLECFFAVPMMGCVLQTVNVRLSPEQIAYTLNHA 111 R AN L+ GV G+ + + + A +G + + P+ IAY L+++ Sbjct: 74 RFANALRAAGVGRGDRIGIYLSQRIETVITHLAAYKLGAITVPLFYLFGPDAIAYRLDNS 133 Query: 112 QADVLLVNSDFMPVLKQIRGELNTLTRCVLLTDDGSPCPQGPGFAGEYEALLAQASAQYD 171 A L+ ++ M + GEL L + PQ F A A+ Sbjct: 134 GAVALVTDASGMEK-ATLAGELPALRLVFCVESSDLVLPQTTDFWDRLHAAPAELDPVLT 192 Query: 172 FPDFDENARATTFYTTGTTGLPKGVYFSHRQLVLHTLGTTAGLALAPQQGRLHRDDVYMP 231 D A YT+GTTG KG +HR L+ H G PQ+G D + P Sbjct: 193 LAD----DPAMIIYTSGTTGKAKGALHAHRVLLGHLPGVEVSHDSFPQEG----DRFWTP 244 Query: 232 LTPMFHVHAWG-------LPYVATMAGVKQVYPGRYVPDTICKLIREEKVTFSHCVPTIL 284 AW LP + V ++ + L+ ++ PT L Sbjct: 245 AD-----WAWIGGLLDVLLPSLYHGVAVVARRLEKFDAAEVFGLLARHQIRNVFFPPTAL 299 Query: 285 QMVLTSEQAR-EVDLSGWKMIIGGSAMTGSLARMARDR-GIDVFTGYGMSETCPILTLAQ 342 +M+ + R + D S + GG + L R+ G+ + YG +E C ++ + Sbjct: 300 KMLRGAATVRAQADFSLRSVASGGETLGDDLIAWGREALGVTINEFYGQTE-CNLVVSSS 358 Query: 343 VPTEDLGSEREAVLRVKTGRPVPLVALRTVDGDMNDCPRDGKSTGEVVVRAP--WLTQGY 400 S + GR VP ++ VD P + G + +RAP + Y Sbjct: 359 -------SHCYPSVSGSMGRAVPGHVVQIVDEQGQVLPH--GTVGNIAIRAPDPVMFLRY 409 Query: 401 LHQAEASEELWRGGWLHTQDIGHLDAEGYLQVTDRIKDVIKTGGEWVSSLQIEDIIARHP 460 EA+ E + G +L T D+G +D +GY++ R DVI + G + IE+ + RHP Sbjct: 410 WRNEEATREKFAGDFLLTGDLGSMDEQGYIRYLGRNDDVITSAGYRIGPAAIEECLMRHP 469 Query: 461 AVAETAVIGVQDGKWGERPVALVVLKKDV--DAVSEEDIRQHVMAAAEAGTISRYGVPDR 518 AV AV+GV+D E A VVLK V D + +++QHV A ++ + P Sbjct: 470 AVRIAAVVGVKDALRTEVVKAFVVLKDGVTPDEALKAELQQHVRA-----QLAAHEYPRL 524 Query: 519 VRFVPELARTSVGKLNKRVMRE 540 + FV L T+ GK+ ++ ++E Sbjct: 525 ISFVAALPTTATGKIMRKTLKE 546 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,713,211 Number of extensions: 71386 Number of successful extensions: 241 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 215 Number of HSP's successfully gapped: 16 Length of query: 546 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 456 Effective length of database: 1,193,720 Effective search space: 544336320 Effective search space used: 544336320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)