BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0085.p (161 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310932|ref|YP_003775024.1| hypothetical protein Hsero_1603... 100 7e-23 >gi|300310932|ref|YP_003775024.1| hypothetical protein Hsero_1603 [Herbaspirillum seropedicae SmR1] Length = 157 Score = 100 bits (248), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 87/136 (63%), Positives = 98/136 (72%), Gaps = 15/136 (11%) Query: 1 MMLRAKLLLLWLLALAIPVQGFAAVLQACAPAQQQAVQHHAPAVQAHQ------MQHEHN 54 +MLRAKL LL LLALAIPVQGFAA LQACAPA Q + +HHAP+ A MQHEH+ Sbjct: 12 IMLRAKLFLLCLLALAIPVQGFAAALQACAPAVQSSARHHAPSAPAAPAAHSAGMQHEHH 71 Query: 55 DQQAHQGHHADHGAATQITSADASDTQHQP---HHTHSSCSFCAACTVGGLLPLALSTPP 111 Q H HGAA Q + +++T HQP HHT +SCSFCAACTVGGLLPLALS PP Sbjct: 72 APQGH------HGAAAQTVADQSAETAHQPDPPHHTQASCSFCAACTVGGLLPLALSAPP 125 Query: 112 APDLPSREFIALCTAG 127 APDLPSREFIAL +G Sbjct: 126 APDLPSREFIALPASG 141 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.132 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 259,204 Number of extensions: 6030 Number of successful extensions: 29 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 27 Number of HSP's successfully gapped: 1 Length of query: 161 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 83 Effective length of database: 1,250,540 Effective search space: 103794820 Effective search space used: 103794820 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 127 (53.5 bits)