BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0082.p (1016 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310933|ref|YP_003775025.1| metal-transporting P-type ATPas... 1294 0.0 gi|300309618|ref|YP_003773710.1| LacI family transcription regul... 492 e-140 gi|300310937|ref|YP_003775029.1| heavy metal translocating P-typ... 320 4e-88 gi|300311699|ref|YP_003775791.1| cation transport P-type ATPase ... 224 3e-59 gi|300312503|ref|YP_003776595.1| cation transport P-type ATPase ... 169 1e-42 >gi|300310933|ref|YP_003775025.1| metal-transporting P-type ATPase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 778 Score = 1294 bits (3348), Expect = 0.0, Method: Compositional matrix adjust. Identities = 660/705 (93%), Positives = 681/705 (96%) Query: 37 CCSGHACASNEAAPAPIPAAQLEGKTEEAVFFIQKMDCPTEEKLIRERLSQMSGVGTLEF 96 CC+GHACAS + APIPAA+LEG TEEAVFFIQKMDCPTEEKLIRERLSQMSGVGTLEF Sbjct: 37 CCAGHACASEQERSAPIPAARLEGATEEAVFFIQKMDCPTEEKLIRERLSQMSGVGTLEF 96 Query: 97 NLIQRELTVQHQLPSIAPLVDTLKALDMLPAVKSDSLDAEAGRDAPDQAAAYRIPARRWA 156 NLIQRELTVQHQLPSIAPLV TLKALDMLPAVKSDSLDAEAGRDAPDQAAAYRIPARRW Sbjct: 97 NLIQRELTVQHQLPSIAPLVATLKALDMLPAVKSDSLDAEAGRDAPDQAAAYRIPARRWI 156 Query: 157 LLGLAGVAALGAEVLAWTSGNDHSLPVIALAIAAILLGGLGTLKKGWIALRNFSLNMNFL 216 LLGLAGVAALGAEVLAW+SG++ SLPVI LA+AAILLGGLGTLKKGWIALRNFSLNMNFL Sbjct: 157 LLGLAGVAALGAEVLAWSSGDERSLPVIGLALAAILLGGLGTLKKGWIALRNFSLNMNFL 216 Query: 217 MSLAVIGAAVIGQWPEAAVVIVLFTLAEMIEALSLDRARNAIAGLMAMTPDMATRRGADG 276 MSLAVIGAAVIGQWPEAAVVIVLFTLAEMIEALSLDRARNAIAGL+AMTPDMATRRG DG Sbjct: 217 MSLAVIGAAVIGQWPEAAVVIVLFTLAEMIEALSLDRARNAIAGLLAMTPDMATRRGEDG 276 Query: 277 VWSTVPARTVTVDETVRVAPGERVPLDGLVLSGSTSINQAPITGESIPVQKQPGDPVFAG 336 VWSTVPARTVTVD+TVRVAPGERVPLDGLVLSGSTSINQAPITGESIPVQKQ GDPVFAG Sbjct: 277 VWSTVPARTVTVDDTVRVAPGERVPLDGLVLSGSTSINQAPITGESIPVQKQAGDPVFAG 336 Query: 337 TINEQGAFEMRVTAVQGDSTLARIVKSVQQAQGQRAPTQRFVDNFARYYIPAVVLMALLV 396 TINEQGAFEMRVTA QGDSTLARIVKSVQQAQGQRAPTQRFVDNFARYYIPAVV+MA+LV Sbjct: 337 TINEQGAFEMRVTAAQGDSTLARIVKSVQQAQGQRAPTQRFVDNFARYYIPAVVVMAVLV 396 Query: 397 AVLPPLLMGAPFQPWLYKALVLLVIACPCALVISTPVTIVSGLASAARHGILVKGGVYLE 456 A+LPPLL GA FQPWLYKALVLLVIACPCALVISTPVTIVSGLASAARHGILVKGGVYLE Sbjct: 397 ALLPPLLAGAAFQPWLYKALVLLVIACPCALVISTPVTIVSGLASAARHGILVKGGVYLE 456 Query: 457 QGRQLRALALDKTGTITFGKPAVTDVVSLQAGADQQALLQLAVSLAARSDHPVSRAIAQQ 516 QGRQLRALALDKTGT+TFGKP VTDVVSLQA ADQQALLQLAVSLAARSDHPVSRAIA Q Sbjct: 457 QGRQLRALALDKTGTLTFGKPRVTDVVSLQAQADQQALLQLAVSLAARSDHPVSRAIAAQ 516 Query: 517 EDIVTLEVSDFAALAGRGVQGRIGGQLLHLGNHRLVHELGQCSPELEARLQAFEKQGKTT 576 E+I T+EVS+FAALAGRGVQGRI GQLLHLGNHRLVHELGQCSPELEARLQAFEKQGKTT Sbjct: 517 EEIATVEVSEFAALAGRGVQGRINGQLLHLGNHRLVHELGQCSPELEARLQAFEKQGKTT 576 Query: 577 TVLCRDGQPQLLVAVADTVRPSSKEAVAELRALGVDVIMLTGDNSHTAQAIASQTGIADA 636 TVLC DGQPQLLVAVADTVRPSS++AVAELRALGV+VIMLTGDNSHTAQAIA+QTGI+DA Sbjct: 577 TVLCLDGQPQLLVAVADTVRPSSRQAVAELRALGVEVIMLTGDNSHTAQAIAAQTGISDA 636 Query: 637 RGDQLPEDKLKAIEALSARHGVRGVGMVGDGINDAPALARADIGFAMGAAGTDTALETAD 696 RGDQLPEDKLKAIE LS RHGVRGVGMVGDGINDAPALARADIGFAMGAAGTDTALETAD Sbjct: 637 RGDQLPEDKLKAIEELSQRHGVRGVGMVGDGINDAPALARADIGFAMGAAGTDTALETAD 696 Query: 697 VALMDDDLRKIPDFIRLSRKAHTVLVQNISIALGIKAVFLALALV 741 VALMDDDLRKIPDFIRLSRKAH VLVQNISIALGIKAVFL LALV Sbjct: 697 VALMDDDLRKIPDFIRLSRKAHAVLVQNISIALGIKAVFLGLALV 741 >gi|300309618|ref|YP_003773710.1| LacI family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 338 Score = 492 bits (1267), Expect = e-140, Method: Compositional matrix adjust. Identities = 238/277 (85%), Positives = 252/277 (90%) Query: 738 LALVVSSIANPFYPEFALAAERAARERGRFLIVCNTNDDPDTGRAYLDQIAGTLSDGVLI 797 LALVVSSIANPFYPEFALAAERAAR+ GRFLIVCNTNDDPD GRAYLDQIAGTLSDGVLI Sbjct: 62 LALVVSSIANPFYPEFALAAERAARDHGRFLIVCNTNDDPDIGRAYLDQIAGTLSDGVLI 121 Query: 798 MNANLPMDELKKTERRGAPVVLCMWEKPDAPPELPCIAVDFYAAGATAAQHLLGLGHRHI 857 MNANLPMDEL+KTE+RGAPVVLCMWEKPDAPPELPCIAVDF+ AGA AA+HLLGLGH+ Sbjct: 122 MNANLPMDELRKTEQRGAPVVLCMWEKPDAPPELPCIAVDFFEAGAIAARHLLGLGHQCF 181 Query: 858 GALVGSKKSGIHAERYRGFVEACAAAGVKHTLQSARYIGDTVEAGYQATIALLREEPQLT 917 ALVGSKKSGIHAERYRGF AC AG H Q RYI DTVE+GY+AT+ LLRE PQ+T Sbjct: 182 AALVGSKKSGIHAERYRGFASACKEAGHPHPTQRTRYINDTVESGYRATMELLREHPQVT 241 Query: 918 AIFASNDLPALGAMNAIADLGMNVPRDISVIGITDIQWARVSRPALTTVAVPTEEAARMS 977 AIFASNDLPALGAMNAIAD+GMNVPRD+SV GITDIQWARVSRPALTTVAVPTE AARMS Sbjct: 242 AIFASNDLPALGAMNAIADMGMNVPRDVSVTGITDIQWARVSRPALTTVAVPTEAAARMS 301 Query: 978 IDLLLALIEGRSPSPVMRVTATPQLVQRASTAVPRKR 1014 I+LLLALIE RSPSPVM VTA PQLV+RASTAVPRKR Sbjct: 302 IELLLALIERRSPSPVMHVTAAPQLVERASTAVPRKR 338 >gi|300310937|ref|YP_003775029.1| heavy metal translocating P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 744 Score = 320 bits (820), Expect = 4e-88, Method: Compositional matrix adjust. Identities = 224/584 (38%), Positives = 329/584 (56%), Gaps = 38/584 (6%) Query: 202 GWIALRNFSLNMNFLMSLAVIGAAVIGQW--------------------PEAAVVIVLFT 241 GW ALR S NM+ L++L A + W +AVVI L Sbjct: 144 GWGALRARSGNMDLLVALGTSAAYGLSLWQWLTLPTMPGHAMAMPHLYFESSAVVITLVL 203 Query: 242 LAEMIEALSLDRARNAIAGLMAMTPDMATRRGADGVWSTVPARTVTVDETVRVAPGERVP 301 L + +E + + +AIA L A+ P+ A R DG VP V V + V + PGERVP Sbjct: 204 LGKWLEGRAKRQTADAIAALNALRPENARVR-RDGQDIEVPLAQVRVGDLVVLRPGERVP 262 Query: 302 LDGLVLSGSTSINQAPITGESIPVQKQPGDPVFAGTINEQGAFEMRVTAVQGDSTLARIV 361 +DG+V G + +++A +TGES P KQ GDP+ AG+IN +G M TA+ ++ LARIV Sbjct: 263 VDGVVREGRSHLDEAMLTGESRPQAKQAGDPLTAGSINAEGVLVMETTAIGAETVLARIV 322 Query: 362 KSVQQAQGQRAPTQRFVDNFARYYIPAVVLMALLVAVLPPLLMGAPFQPWLYKALVLLVI 421 + V+QAQ +AP QR VD + ++PAV+L++LL + L+ G Q L A+ ++VI Sbjct: 323 RMVEQAQAAKAPIQRLVDQVSAVFVPAVLLLSLLTLLGWGLVQGNWEQA-LLNAVAVMVI 381 Query: 422 ACPCALVISTPVTIVSGLASAARHGILVKGGVYLEQGRQLRALALDKTGTITFGKPAVTD 481 ACPCAL ++TP I++G AARHGIL+K LE ++ A+A DKTGT+T G P Sbjct: 382 ACPCALGLATPTAIMAGTGVAARHGILIKDAEALEIAHRVNAVAFDKTGTLTLGHP---R 438 Query: 482 VVSLQAGADQQ--ALLQLAVSLAARSDHPVSRAIAQQEDIVTLEVSDFA---ALAGRGVQ 536 ++ L+A Q LL LA +L A S+HP+ RA+ + ++AG G++ Sbjct: 439 LLELEATPSTQPDELLALAAALQAHSEHPLGRAVREAAAQSAQAARQAEQVRSVAGLGIE 498 Query: 537 GRIGGQLLHLGNHRLVHELGQCSPELEARLQAFEKQGKTTTVLCRDGQPQL----LVAVA 592 G + G+ L LG+ R + +L +P L+AR + + QG+T + L R + L+A Sbjct: 499 GVVEGRQLMLGSSRWMQQLQAHAPALQARAEQLQAQGRTLSWLARRNDSGVEVLGLLAFG 558 Query: 593 DTVRPSSKEAVAELRALGVDVIMLTGDNSHTAQAIASQTGIADARGDQLPEDKLKAIEAL 652 D ++P ++ A+A L +GV ML+GDN AQ +AS+ GI + R + LP DK +A+ AL Sbjct: 559 DQIKPGAQAAIATLHRMGVRSAMLSGDNLGAAQQVASELGIQEVRAEVLPADKAEAVTAL 618 Query: 653 SARHGVRGVGMVGDGINDAPALARADIGFAMGAAGTDTALETADVALMDDDLRKIPDFIR 712 + G + V MVGDG+NDAPALA AD+G AM + GTD A++ A V LM D + D I Sbjct: 619 R-QGGAQVVAMVGDGVNDAPALAAADVGMAM-STGTDVAMQAAGVTLMHGDPGLVADAID 676 Query: 713 LSRKAHTVLVQNISIALGIKAVFLALALVVSSIANPFYPEFALA 756 +SR+ + + QN+ A V + LA + + NP A+A Sbjct: 677 ISRRTYAKIRQNLFWAFIYNLVGIPLAAL--GLLNPMLAGAAMA 718 >gi|300311699|ref|YP_003775791.1| cation transport P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 734 Score = 224 bits (571), Expect = 3e-59, Method: Compositional matrix adjust. Identities = 188/568 (33%), Positives = 305/568 (53%), Gaps = 39/568 (6%) Query: 172 AWTSGNDHSLPVIALAIAAILLGGLGTLKKGWIALRNFSLNMNFLMSLAVIGAAVIGQWP 231 AW +G P++ L + L +A+R + ++ + +++ GA ++ + Sbjct: 10 AWLAG-----PLVVL---------MAVLADTAMAMRRGGMKLDLIALVSIAGAVLLREDL 55 Query: 232 EAAVVIVLFTLAEMIEALSLDRARNAIAGLMAMTPDMATRRGADGVWSTVPARTVTVDET 291 AAV+ ++F +E+++ RA+ ++ L+ P +A R + + T+ V + Sbjct: 56 VAAVIALMFAGGRALESVAQRRAQQEMSALLGKAPHVANRYEGERI-VTIGLNLVRPGDR 114 Query: 292 VRVAPGERVPLDGLVLSGSTSINQAPITGESIPVQKQPGDPVFAGTINEQGAFEMRVTAV 351 + V PGE VP+DG +L G ++++ +TGES+PV G + +G +N AF++ T+ Sbjct: 115 LLVRPGEPVPVDGRLL-GPAVVDESMLTGESLPVSHPQGATLASGCVNAGNAFDLLATST 173 Query: 352 QGDSTLARIVKSVQQAQGQRAPTQRFVDNFARYYIPAVVLMALLVAVLPPLLMGAPFQPW 411 ST A++++ VQ AQ +AP R D +A +IP V+ MA L LL G P Sbjct: 174 DQSSTFAQVLRLVQAAQASQAPAARMADRYALLFIPLVLAMA----ALSWLLTGDP---- 225 Query: 412 LYKALVLLVIACPCALVISTPVTIVSGLASAARHGILVKGGVYLEQGRQLRALALDKTGT 471 +AL ++V+A PC L+++ PV IV ++ A+ G+L+K G LE+ Q+R L DKTGT Sbjct: 226 -RRALAVVVVATPCPLILAVPVAIVCAMSRCAQRGVLIKHGGALEKLAQVRTLFFDKTGT 284 Query: 472 ITFGKPAVTDVV---SLQAGADQQALLQLAVSLAARSDHPVSRAI--AQQEDIVTLEVSD 526 +T GK + ++V S+QA LL+LA SL S+H +++A+ A E + L + D Sbjct: 285 LTAGKARLMNMVTTPSIQA----DELLRLAASLEQMSNHVIAQAVVSAAIERALVLSIPD 340 Query: 527 -FAALAGRGVQGRIGGQLLHLGNHRLVHELGQCSPELEARLQAFEKQGKTTTVLCRDGQP 585 G G+ GR+ G+L+ +G + V P + +G + DGQ Sbjct: 341 QVQEQPGAGLSGRVDGKLVRVGAMQYVIGPSAAPPWTSGLRDRLKYEGGAGVCVAVDGQL 400 Query: 586 QLLVAVADTVRPSSKEAVAELRALGVD-VIMLTGDNSHTAQAIASQTGIADARGDQLPED 644 ++ +AD +R + A+ +LRALG ++MLTGD A I S G D +Q P Sbjct: 401 CGVLYLADDIRLDTPRALRQLRALGAKRIVMLTGDRRDVAMTIGSSLGFDDVMPEQSPAS 460 Query: 645 KLKAIEALSARHGVRGVGMVGDGINDAPALARADIGFAMGAAGTDTALETADVALMDDDL 704 KL AI+ S R GV MVGDGINDAPALA ADIG AMGA G A E+A V L+ D L Sbjct: 461 KLAAIKQAS-REGV--TMMVGDGINDAPALAAADIGVAMGARGAAAAAESAQVVLLVDRL 517 Query: 705 RKIPDFIRLSRKAHTVLVQNISIALGIK 732 ++ +R++++A + +Q++ +G+ Sbjct: 518 DRLAFAVRMAQQARKIALQSVVAGMGLS 545 >gi|300312503|ref|YP_003776595.1| cation transport P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 839 Score = 169 bits (428), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 140/467 (29%), Positives = 226/467 (48%), Gaps = 36/467 (7%) Query: 296 PGERVPLDGLVLSGSTSINQAPITGESIPVQKQPGDPVFAGTINEQGAFEMRVTAVQGDS 355 PG+ + D +L G+ + A ++GES P Q G G +N +RV G+S Sbjct: 352 PGDTIAADACLLEGAGEFDLALLSGESRPQSFQIGQEAPGGAVNLSQPVFLRVVRATGES 411 Query: 356 TLARIVKSVQQA-QGQRAPTQRFVDNFARYYIPAVVLMALLVAVLPPLLMGAPFQPW--- 411 TLA + + +QA QG+ A Q + D A ++ A++L+A GA F W Sbjct: 412 TLAALTRLAEQAGQGKPALAQ-WADVVASRFVVALLLLA-----------GATFLAWQLI 459 Query: 412 -----LYKALVLLVIACPCALVISTPVTIVSGLASAARHGILVKGGVYLEQGRQLRALAL 466 A+ +LV++CPCAL ++TP + + R G L+ G LE ++ + Sbjct: 460 DPTRAWSTAIAVLVVSCPCALSLATPSALAAATDRLLRAGTLIVRGNVLETLQRADTIVF 519 Query: 467 DKTGTITFGKPAVTDVVSLQAGADQQALLQLAVSLAARSDHPVSRAIAQQ---EDIVTLE 523 DKTGT+T G+P +T +L + D Q L +A ++ S HP+++A+ ++ + LE Sbjct: 520 DKTGTLTQGRPQLT---ALWSDVDCQRSLAIAAAMERGSLHPLAKALVEEGARRQVDALE 576 Query: 524 VSDFAALAGRGVQGRIGGQLLHLGNHRLVHEL-GQCSPELEARLQAFEKQGKTTTVLCRD 582 V ++ G G+QG I G L +G+ V L G P + LQ+ + + D Sbjct: 577 VEQLTSVTGAGMQGMIEGDLYRIGSRSFVAGLAGTALPPV--LLQSAASGAGSVYLGSSD 634 Query: 583 GQPQLLVAVADTVRPSSKEAVAELRALGVDVIMLTGDNSHTAQAIASQTGIADARGDQLP 642 G +AD +R + VA RALG+ I+L+GD +A+ GI++ + P Sbjct: 635 GWLARF-DLADALRTDAVATVAAFRALGLRTILLSGDQPEVCAEVAAAAGISEVQAACTP 693 Query: 643 EDKLKAIEALSARHGVRGVGMVGDGINDAPALARADIGFAMGAAGTDTALETADVALMDD 702 + KL + L V V +VGDGINDA L D+ FAMG G A +AD +M D Sbjct: 694 QGKLDYVRNLQRDGAV--VAIVGDGINDAAMLRAGDVSFAMG-KGAALAQVSADAVIMSD 750 Query: 703 DLRKIPDFIRLSRKAHTVLVQNISIALGIKAVFLALALVVSSIANPF 749 L+ + D ++++ ++ QN+ A FLA+ + +P+ Sbjct: 751 RLQAVADCAAMAQRTMHIVRQNLVWAATYN--FLAIPAAAFGLLDPW 795 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,850,126 Number of extensions: 118949 Number of successful extensions: 545 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 512 Number of HSP's successfully gapped: 15 Length of query: 1016 Length of database: 1,619,870 Length adjustment: 95 Effective length of query: 921 Effective length of database: 1,170,045 Effective search space: 1077611445 Effective search space used: 1077611445 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 135 (56.6 bits)