BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0076.p (315 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310937|ref|YP_003775029.1| heavy metal translocating P-typ... 448 e-127 gi|300310933|ref|YP_003775025.1| metal-transporting P-type ATPas... 179 2e-46 gi|300312503|ref|YP_003776595.1| cation transport P-type ATPase ... 144 9e-36 gi|300311691|ref|YP_003775783.1| cation transport ATPase [Herbas... 98 7e-22 gi|300311699|ref|YP_003775791.1| cation transport P-type ATPase ... 95 6e-21 >gi|300310937|ref|YP_003775029.1| heavy metal translocating P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 744 Score = 448 bits (1152), Expect = e-127, Method: Compositional matrix adjust. Identities = 249/315 (79%), Positives = 268/315 (85%), Gaps = 4/315 (1%) Query: 1 VGRPRLTELQAADAAGQDQEHALLALAAALQARSEHPLARAVLEAAAAHTDISIPQAGQV 60 +G PRL EL+A + D+ AL AAALQA SEHPL RAV EAAA + QA QV Sbjct: 434 LGHPRLLELEATPSTQPDELLAL---AAALQAHSEHPLGRAVREAAAQSAQAAR-QAEQV 489 Query: 61 RSVAGLGIEGHVDGRALCLGSSRWMQQLQASAPALQARADQLQAQGRTLSWLAEREAGHV 120 RSVAGLGIEG V+GR L LGSSRWMQQLQA APALQARA+QLQAQGRTLSWLA R V Sbjct: 490 RSVAGLGIEGVVEGRQLMLGSSRWMQQLQAHAPALQARAEQLQAQGRTLSWLARRNDSGV 549 Query: 121 RILGLLAFGDEIKPGAQQAIATLHALGIRTAMLSGDNRGAAQQVAATLGIQDVRAEVLPG 180 +LGLLAFGD+IKPGAQ AIATLH +G+R+AMLSGDN GAAQQVA+ LGIQ+VRAEVLP Sbjct: 550 EVLGLLAFGDQIKPGAQAAIATLHRMGVRSAMLSGDNLGAAQQVASELGIQEVRAEVLPA 609 Query: 181 DKAEAVAALRENGAHVVAMVGDGVNDAPALAAADVGIAMSTGTDVAMQAAGITLMHGDPG 240 DKAEAV ALR+ GA VVAMVGDGVNDAPALAAADVG+AMSTGTDVAMQAAG+TLMHGDPG Sbjct: 610 DKAEAVTALRQGGAQVVAMVGDGVNDAPALAAADVGMAMSTGTDVAMQAAGVTLMHGDPG 669 Query: 241 LAADAIDISRRTYGKIRQNLFWAFIYNLVGIPLAALGLLNPMLAGAAMAFSSVSVVSNAL 300 L ADAIDISRRTY KIRQNLFWAFIYNLVGIPLAALGLLNPMLAGAAMAFSSVSVVSNAL Sbjct: 670 LVADAIDISRRTYAKIRQNLFWAFIYNLVGIPLAALGLLNPMLAGAAMAFSSVSVVSNAL 729 Query: 301 LLRRWRPHGPAKMEK 315 LLRRWRPH P ++ Sbjct: 730 LLRRWRPHAPVSTKE 744 >gi|300310933|ref|YP_003775025.1| metal-transporting P-type ATPase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 778 Score = 179 bits (455), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 118/305 (38%), Positives = 182/305 (59%), Gaps = 12/305 (3%) Query: 2 GRPRLTELQAADAAGQDQEHALLALAAALQARSEHPLARAVLEAAAAHTDISIPQAGQVR 61 G+PR+T++ + A Q + ALL LA +L ARS+HP++RA+ AA +I+ + + Sbjct: 475 GKPRVTDVVSLQA--QADQQALLQLAVSLAARSDHPVSRAI----AAQEEIATVEVSEFA 528 Query: 62 SVAGLGIEGHVDGRALCLGSSRWMQQLQASAPALQARADQLQAQGRTLSWLAEREAGHVR 121 ++AG G++G ++G+ L LG+ R + +L +P L+AR + QG+T + L G + Sbjct: 529 ALAGRGVQGRINGQLLHLGNHRLVHELGQCSPELEARLQAFEKQGKTTTVLCLD--GQPQ 586 Query: 122 ILGLLAFGDEIKPGAQQAIATLHALGIRTAMLSGDNRGAAQQVAATLGIQDVRAEVLPGD 181 +L +A D ++P ++QA+A L ALG+ ML+GDN AQ +AA GI D R + LP D Sbjct: 587 LL--VAVADTVRPSSRQAVAELRALGVEVIMLTGDNSHTAQAIAAQTGISDARGDQLPED 644 Query: 182 KAEAVAALRE-NGAHVVAMVGDGVNDAPALAAADVGIAM-STGTDVAMQAAGITLMHGDP 239 K +A+ L + +G V MVGDG+NDAPALA AD+G AM + GTD A++ A + LM D Sbjct: 645 KLKAIEELSQRHGVRGVGMVGDGINDAPALARADIGFAMGAAGTDTALETADVALMDDDL 704 Query: 240 GLAADAIDISRRTYGKIRQNLFWAFIYNLVGIPLAALGLLNPMLAGAAMAFSSVSVVSNA 299 D I +SR+ + + QN+ A V + LA +G+ + +A A +S+ VV N Sbjct: 705 RKIPDFIRLSRKAHAVLVQNISIALGIKAVFLGLALVGMSSLWMAVFADMGASLIVVFNG 764 Query: 300 LLLRR 304 L L R Sbjct: 765 LRLVR 769 >gi|300312503|ref|YP_003776595.1| cation transport P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 839 Score = 144 bits (363), Expect = 9e-36, Method: Compositional matrix adjust. Identities = 114/305 (37%), Positives = 161/305 (52%), Gaps = 17/305 (5%) Query: 2 GRPRLTELQAADAAGQDQEHALLALAAALQARSEHPLARAVLEAAAAHTDISIPQAGQVR 61 GRP+LT L + D Q LA+AAA++ S HPLA+A++E A + + Q+ Sbjct: 528 GRPQLTALWS-DVDCQRS----LAIAAAMERGSLHPLAKALVEEGA-RRQVDALEVEQLT 581 Query: 62 SVAGLGIEGHVDGRALCLGSSRWMQQLQASA--PALQARADQLQAQGRTLSWLAEREAGH 119 SV G G++G ++G +GS ++ L +A P L Q A G +L + Sbjct: 582 SVTGAGMQGMIEGDLYRIGSRSFVAGLAGTALPPVLL----QSAASGAGSVYLGSSDGWL 637 Query: 120 VRILGLLAFGDEIKPGAQQAIATLHALGIRTAMLSGDNRGAAQQVAATLGIQDVRAEVLP 179 R D ++ A +A ALG+RT +LSGD +VAA GI +V+A P Sbjct: 638 AR----FDLADALRTDAVATVAAFRALGLRTILLSGDQPEVCAEVAAAAGISEVQAACTP 693 Query: 180 GDKAEAVAALRENGAHVVAMVGDGVNDAPALAAADVGIAMSTGTDVAMQAAGITLMHGDP 239 K + V L+ +GA VVA+VGDG+NDA L A DV AM G +A +A +M Sbjct: 694 QGKLDYVRNLQRDGA-VVAIVGDGINDAAMLRAGDVSFAMGKGAALAQVSADAVIMSDRL 752 Query: 240 GLAADAIDISRRTYGKIRQNLFWAFIYNLVGIPLAALGLLNPMLAGAAMAFSSVSVVSNA 299 AD +++RT +RQNL WA YN + IP AA GLL+P ++ M+ SS+ VV NA Sbjct: 753 QAVADCAAMAQRTMHIVRQNLVWAATYNFLAIPAAAFGLLDPWMSAVGMSLSSLLVVGNA 812 Query: 300 LLLRR 304 L L R Sbjct: 813 LRLSR 817 >gi|300311691|ref|YP_003775783.1| cation transport ATPase [Herbaspirillum seropedicae SmR1] Length = 853 Score = 98.2 bits (243), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 71/193 (36%), Positives = 101/193 (52%), Gaps = 33/193 (17%) Query: 120 VRILGLLAFGDEIKPGAQQAIATLHALGIRTAMLSGDNRGAAQQVAATLGIQ-------- 171 +R LGLLA D ++PG AI H IR M++GD+ AQ +A GI Sbjct: 481 LRYLGLLALADPLRPGVPDAIQRCHQAEIRVIMITGDHPSTAQNIARQAGISADQVLLGD 540 Query: 172 -----------------DVRAEVLPGDKAEAVAALRENGAHVVAMVGDGVNDAPALAAAD 214 +V A + P K VAAL+++G ++AM GDGVNDAPAL AA Sbjct: 541 EVQAMAEHELARRLRTTNVCARITPLQKLRIVAALKDDG-EIIAMTGDGVNDAPALKAAH 599 Query: 215 VGIAM-STGTDVAMQAAGITLMHGDPGLAADAIDISRRTYGKIRQNLFWAFIYNL-VGIP 272 VGIAM + GTDVA +A + L+ + + I + RR + ++R A IY L + +P Sbjct: 600 VGIAMGARGTDVAREAGSLILLDDNFASIVEGILMGRRIFSRMRG----AMIYVLAMHMP 655 Query: 273 LAALGLLNPMLAG 285 +A + LL P++ G Sbjct: 656 IAGMALL-PVVFG 667 >gi|300311699|ref|YP_003775791.1| cation transport P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 734 Score = 95.1 bits (235), Expect = 6e-21, Method: Compositional matrix adjust. Identities = 76/216 (35%), Positives = 114/216 (52%), Gaps = 13/216 (6%) Query: 2 GRPRLTELQAADAAGQDQEHALLALAAALQARSEHPLARAVLEAAAAHTDI-SIPQAGQV 60 G+ RL + + D+ LL LAA+L+ S H +A+AV+ AA + SIP QV Sbjct: 288 GKARLMNMVTTPSIQADE---LLRLAASLEQMSNHVIAQAVVSAAIERALVLSIPD--QV 342 Query: 61 RSVAGLGIEGHVDGRALCLGSSRWMQQLQASAPALQARADQLQAQGRTLSWLAEREAGHV 120 + G G+ G VDG+ + +G+ +++ A+ P D+L+ +G +A Sbjct: 343 QEQPGAGLSGRVDGKLVRVGAMQYVIGPSAAPPWTSGLRDRLKYEGGAGVCVAVDG---- 398 Query: 121 RILGLLAFGDEIKPGAQQAIATLHALGI-RTAMLSGDNRGAAQQVAATLGIQDVRAEVLP 179 ++ G+L D+I+ +A+ L ALG R ML+GD R A + ++LG DV E P Sbjct: 399 QLCGVLYLADDIRLDTPRALRQLRALGAKRIVMLTGDRRDVAMTIGSSLGFDDVMPEQSP 458 Query: 180 GDKAEAVAALRENGAHVVAMVGDGVNDAPALAAADV 215 K A+ G V MVGDG+NDAPALAAAD+ Sbjct: 459 ASKLAAIKQASREG--VTMMVGDGINDAPALAAADI 492 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.131 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 929,101 Number of extensions: 36908 Number of successful extensions: 235 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 218 Number of HSP's successfully gapped: 7 Length of query: 315 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 230 Effective length of database: 1,217,395 Effective search space: 280000850 Effective search space used: 280000850 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)