BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0058.p (417 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310957|ref|YP_003775049.1| branched-chain amino acid ABC t... 668 0.0 gi|300311772|ref|YP_003775864.1| branched-chain amino acid ABC t... 345 3e-96 gi|300312955|ref|YP_003777047.1| amino acid ABC transporter peri... 99 5e-22 gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC t... 86 4e-18 gi|300312236|ref|YP_003776328.1| amino acid ABC transporter peri... 86 5e-18 >gi|300310957|ref|YP_003775049.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 413 Score = 668 bits (1724), Expect = 0.0, Method: Compositional matrix adjust. Identities = 321/380 (84%), Positives = 356/380 (93%) Query: 38 AVEPIRIGEINSQGRASTAGQAYRNGLSLALEQINESGGIHGRPLQLISRDDQGDADQAA 97 A E IRIGEINS+ R STAGQAYRNGL+LALEQIN +GG+ GRPLQL+ R+D GDADQAA Sbjct: 34 AAEAIRIGEINSRHRLSTAGQAYRNGLALALEQINAAGGVGGRPLQLVVREDDGDADQAA 93 Query: 98 RNARELVSKEHVEVLTGTMLSDVALAVAREAARSKTVLVVGDALSDAVTLDKGNRYTFRV 157 RNAR L+S++HVEVLTG+MLSDVALAV+REAAR +TVLVVGDALSDA+TLDKGNRYTFRV Sbjct: 94 RNARALISQDHVEVLTGSMLSDVALAVSREAARGRTVLVVGDALSDAITLDKGNRYTFRV 153 Query: 158 RPSTYMLAAMLAEQAARMPGRRWAMVAPNYEYGQSAVASFKMLLKQARPDVEFVSDQWPA 217 RPSTYM AAMLAEQAAR+PGRRWA+VAPNYEYGQSAVASFK+LLKQARPDVEFVSDQWPA Sbjct: 154 RPSTYMQAAMLAEQAARLPGRRWAVVAPNYEYGQSAVASFKLLLKQARPDVEFVSDQWPA 213 Query: 218 MGKIKADDVARAIRQSRPDAIFNATFGEDLQKFVQAGNRQQLFAGVRVVSMSSAGAQSLD 277 +GKIKADDVARAIRQSRPD IFNATFGEDLQKFVQAGNRQQLF GVRVVSM+SAGA SLD Sbjct: 214 LGKIKADDVARAIRQSRPDGIFNATFGEDLQKFVQAGNRQQLFEGVRVVSMASAGAPSLD 273 Query: 278 PSQSEAIRDWVAVGYPWDQINTPEHQRFLEAYLKKFSERPQLSAVLGYSTMMAIAGGLKK 337 PSQSEAIRDW+A+GYPWDQ++ PEHQRFLEAYLK+FSERPQLSAV GY+TMMAIA GLKK Sbjct: 274 PSQSEAIRDWLAIGYPWDQVSLPEHQRFLEAYLKRFSERPQLSAVFGYTTMMAIAAGLKK 333 Query: 338 SEGGDGEVLLESLRGLHWSSPLGELSFRALDQQSTMGAFIGLVSQKDGRSTMREWRYLDG 397 SEGGDGEVL+E++RGL + SPLG +SFRALDQQ+++GAF+GLVSQKDGRS+MR+WRYLDG Sbjct: 334 SEGGDGEVLVEAMRGLQFGSPLGPVSFRALDQQASLGAFVGLVSQKDGRSSMRDWRYLDG 393 Query: 398 TAYLPPPTYVRGRRPASAGK 417 AYLPPP YVR RRPASA K Sbjct: 394 AAYLPPPAYVRSRRPASASK 413 >gi|300311772|ref|YP_003775864.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 345 bits (886), Expect = 3e-96, Method: Compositional matrix adjust. Identities = 168/374 (44%), Positives = 246/374 (65%) Query: 38 AVEPIRIGEINSQGRASTAGQAYRNGLSLALEQINESGGIHGRPLQLISRDDQGDADQAA 97 A + IRIGEINS + YR G +AL++IN SGG+ G+ L++I+RDD G+ + Sbjct: 28 AQDTIRIGEINSYKAQPAFLEPYRRGWEMALDEINASGGVLGKKLEVIARDDNGNPGDSV 87 Query: 98 RNARELVSKEHVEVLTGTMLSDVALAVAREAARSKTVLVVGDALSDAVTLDKGNRYTFRV 157 R A EL+++E V +L G LS+ LA+ A + + + + L+D + GN+YT+R+ Sbjct: 88 RVAEELMARERVALLFGGFLSNTGLALTDYAKQHRVFFLAAEPLTDKIVWQNGNKYTYRL 147 Query: 158 RPSTYMLAAMLAEQAARMPGRRWAMVAPNYEYGQSAVASFKMLLKQARPDVEFVSDQWPA 217 RPST ML A + ++A ++ +RWA+V PNYEYGQSAVASFK L+KQA+PDVEFV++Q P Sbjct: 148 RPSTSMLVASVVKEAVKLKKKRWAIVYPNYEYGQSAVASFKTLMKQAQPDVEFVAEQAPP 207 Query: 218 MGKIKADDVARAIRQSRPDAIFNATFGEDLQKFVQAGNRQQLFAGVRVVSMSSAGAQSLD 277 +GKI A V +A+ ++PDAIFNA F DL KFV+ GN + LF V VVS+ + + LD Sbjct: 208 LGKIDAGAVTQALADAKPDAIFNALFAADLGKFVREGNTRGLFEQVDVVSLLTGEPEYLD 267 Query: 278 PSQSEAIRDWVAVGYPWDQINTPEHQRFLEAYLKKFSERPQLSAVLGYSTMMAIAGGLKK 337 P + EA ++W+ GYPW I+TPEH++F+EAY KKF++ P+ +++GYS +M+IA GL+K Sbjct: 268 PLKGEAPQNWIVTGYPWYAIHTPEHKKFVEAYRKKFNDYPRNGSLVGYSALMSIAAGLRK 327 Query: 338 SEGGDGEVLLESLRGLHWSSPLGELSFRALDQQSTMGAFIGLVSQKDGRSTMREWRYLDG 397 + D + L + GL +P+ + +R D QST+GAF+G K+GR M + Y+DG Sbjct: 328 AGSTDADRLAAAFSGLKVETPVASIVYRPQDHQSTLGAFVGRTGLKEGRGVMTSFTYIDG 387 Query: 398 TAYLPPPTYVRGRR 411 A P V+ R Sbjct: 388 AALQLPDAEVKKLR 401 >gi|300312955|ref|YP_003777047.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 392 Score = 99.4 bits (246), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 101/362 (27%), Positives = 175/362 (48%), Gaps = 23/362 (6%) Query: 14 LSLLPTLLGALAVAVSLVLPLSVGAVEPIRIGEINS-QGRASTAGQAYRNGLSLALEQI- 71 ++ L + GA+ +A+++ L A + +++G + + G AS G R G LAL+ + Sbjct: 1 MNRLSKVAGAVMMAMAVGASLPAQAADVVKVGFLTTLSGPASAPGVDEREGFMLALKTLG 60 Query: 72 NESGGIHGRPLQLISRDDQGDADQAARNARELVSKEHVEVLTGTMLSDVALAVAREAARS 131 N+ GG+ P ++I DDQ D A + A L+ +E V++L+G + S++ LAVA A S Sbjct: 61 NKLGGV---PAEVIVVDDQFSPDAAKQGADRLIKREKVDILSGFVYSNILLAVAPAAFAS 117 Query: 132 KTVLVVGDALSDAVTLDKGNRYTFRVRPSTYMLAAMLAEQAARMPGRRWAMVAPNYEYGQ 191 KTV+V +A + N Y F + A +R A++APNY G+ Sbjct: 118 KTVVVSANAGPAQLAGAGCNPYFFSASYQNDAAHEAAGKLMADKGYQRVAIIAPNYPAGK 177 Query: 192 SAVASFKMLLKQARPDVEFVSDQWPAMGKIK-ADDVARAIRQSRPDAIFNATFG----ED 246 A A FK LK+ P VE +P +G++ A ++A+ +R + DA++ G Sbjct: 178 DASAGFKRFLKK-DPAVEL----FPKLGQLDFATEIAQ-LRAANIDALYFFLPGGMGVNF 231 Query: 247 LQKFVQAGNRQQLFAGVRVVSMSSAGAQSLDPSQSEAIRDWVAVGYPWDQINTPEHQRFL 306 ++++ AG ++ VRV+ A Q L S + I G + +++ F+ Sbjct: 232 VKQYAAAGMDPKI---VRVMPAYDADQQML--SNGDLIAGVANTGQWSSDLPNEQNRAFV 286 Query: 307 EAYLKKFSERPQLSAVLGYSTMMAIAGGLKKSEGG--DGEVLLESLRGLHWSSPLGELSF 364 AYLK++ +P + A Y M I +K G + E L+ +++ ++ S G F Sbjct: 287 AAYLKEYGRQPSMFASQAYDAAMLIDSAVKAVGGKVENREALIAAIKTANFKSVRGGFRF 346 Query: 365 RA 366 A Sbjct: 347 AA 348 >gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 383 Score = 86.3 bits (212), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 92/347 (26%), Positives = 167/347 (48%), Gaps = 26/347 (7%) Query: 23 ALAVAVSLVLPLSVGAVEPIRIGEINS-QGRASTAGQAYRNGLSLALEQINESGGIHGRP 81 A A + L L+ A +PI+IG G ++ G + R+G+ LA+ +IN +GG+ GR Sbjct: 7 AFVAAALMSLALTSQAADPIKIGVSGPYTGGSAPMGVSMRDGVKLAVAEINAAGGLLGRQ 66 Query: 82 LQLISRDDQGDADQAARNARELVSKEHVEVLTGTMLSDVALAVAREAARSKTVLV----V 137 +QLI RDD+ ++ + A+EL++KE V G + + VALA R +K ++ Sbjct: 67 IQLIERDDEAKNERGVQIAQELINKEKVVATVGFINTGVALASQRFYQEAKIPVINNVAT 126 Query: 138 GDALSDAVTLDKGNRYTFRVRPSTYMLAAMLAEQAA-RMPGRRWAMVAPNYEYGQSAVAS 196 G ++ +K N Y FR + + A M+A++A + + A++A + YGQ Sbjct: 127 GTVITKQFEKEKDN-YVFRTSAADKIQAKMIADEAVDKQKFTKVAILADSTNYGQLGRED 185 Query: 197 FKMLL--KQARPDVEFVSDQWPAMGKIKADDVARAIRQSR---PDAIFNATFGEDLQKFV 251 + +L K+ P +++ IK D+ + +S+ A+ G +L + Sbjct: 186 LEKVLADKKVTP---VAVEKY----NIKDVDMTAQLLKSKQGGAQAVLTYGIGPELAQI- 237 Query: 252 QAGNRQQLFAGVRVV-SMSSAGAQSLDPSQSEAIRDWVAVGYPWDQINTPEHQRFLEAYL 310 A ++L V ++ S + +D S + + D NTP+ + F+EAY Sbjct: 238 -ANGMEKLNWHVPIIGSWPLSMGNFIDNSGKNGNGARMPQTFIQDG-NTPKRKAFIEAYQ 295 Query: 311 K--KFSERPQ-LSAVLGYSTMMAIAGGLKKSEGGDGEVLLESLRGLH 354 K K P +SA GY +++ +A +K++ DG+ + +L L+ Sbjct: 296 KAYKVDRMPSAVSAAQGYDSILLLAAAIKQAGTTDGDKVRAALENLN 342 >gi|300312236|ref|YP_003776328.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 85.9 bits (211), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 95/368 (25%), Positives = 159/368 (43%), Gaps = 35/368 (9%) Query: 14 LSLLPTLL-GALAVAVSLVLPLSVGAVEPIRIGEINSQGRASTAGQAYRNGLSLALEQIN 72 L +P LL GA +L + +G E + G A+ G A +NG +LA E+IN Sbjct: 7 LKTIPALLIGATLSQAALAADIKLGVAEALT-------GPAAKYGVAIKNGFTLASEEIN 59 Query: 73 ESGGIHGRPLQLISRDDQGDADQAARNARELVSKEHVEVLTGTMLSDVALAVAREAARSK 132 GG++G L L+ D+QG ++A ++L+ ++ V + G LS+ A A A +K Sbjct: 60 AKGGVNGDKLALVIEDEQGKKEEAINVFKKLIFQDKVLAVFGPTLSNSAFAADPIANAAK 119 Query: 133 TVLVVGDALSDAVTLDKGNRYTFRVRPSTYMLAAML---AEQAARMPG-RRWAMVAPNYE 188 V+ +D +T +TFR ++ M A +L + A + G ++ A++ N + Sbjct: 120 VVVFGTSNTADGIT--AMGPFTFR---NSVMEADVLPVTVKAAVKHFGIKKVAVIYGNDD 174 Query: 189 -YGQSAVASFKMLLKQARPDVEFVSDQWPAMGKIKADDVARAIRQSRPDAIFNATFGEDL 247 + +S FK L+ + + + + A G + I+ PDAI + E+ Sbjct: 175 AFTKSGYDVFKATLEHQK--IPVTTTEAYAKGDVDFKAQLTKIKAGNPDAIVCSCLAEEA 232 Query: 248 QKFVQAGNRQQLFAGVRVVSMSSAGAQSLDPSQSEAIRDW---VAVGYPWDQIN-TPEHQ 303 + Q G++ + G S P E +D +G PW N TP ++ Sbjct: 233 ANII----LQTRALGMKQPFIGGNGLNS--PKLFEIAKDAGDNTLMGSPWSAENQTPANK 286 Query: 304 RFLEAYLKKFSERPQLSAVLGYSTMMAIAGGLKKSE-----GGDGEVLLESLRGLHWSSP 358 F+ AY KF P A Y M +A LK + D + L +L + Sbjct: 287 AFITAYKAKFGADPDQFAAQAYDAMYIVADALKNVKLSGNLAKDRDALRAALPAVKIDGA 346 Query: 359 LGELSFRA 366 G+ +FRA Sbjct: 347 TGKFAFRA 354 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.131 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,185,736 Number of extensions: 46084 Number of successful extensions: 198 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 201 Number of HSP's successfully gapped: 17 Length of query: 417 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 329 Effective length of database: 1,203,190 Effective search space: 395849510 Effective search space used: 395849510 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)