BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0054.p (326 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [H... 643 0.0 gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid... 249 3e-67 gi|300313156|ref|YP_003777248.1| D-2-hydroxyacid dehydrogenase [... 213 3e-56 gi|300311988|ref|YP_003776080.1| D-3-phosphoglycerate dehydrogen... 149 4e-37 gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogen... 144 8e-36 >gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 643 bits (1658), Expect = 0.0, Method: Compositional matrix adjust. Identities = 313/323 (96%), Positives = 318/323 (98%) Query: 1 MKPKIFVARAVFPEVLARLEQHFEVESNQDDRIYSADELLAKANGKDGLFTTPSEPVTAA 60 MKPKIFVARAVFPEVLARLEQHFEVESNQDDRIYSA EL AKA GKDGLFTTPSEPVTAA Sbjct: 1 MKPKIFVARAVFPEVLARLEQHFEVESNQDDRIYSAAELQAKAQGKDGLFTTPSEPVTAA 60 Query: 61 LFAANPQLKAVCNMAVGYNNIDVAAATKAGVMATNTPDVLNETTADFGWALMMAAARRIT 120 LFAANPQLKAVCNMAVGYNNIDVAAAT+AGVMATNTPDVLNETTADFGWALMMAAARRIT Sbjct: 61 LFAANPQLKAVCNMAVGYNNIDVAAATQAGVMATNTPDVLNETTADFGWALMMAAARRIT 120 Query: 121 ESEHFLRAGKWKKWSYDSFVGPDIHGATLGIIGMGRIGQAIARRSLGFDMQVLYHNRSRL 180 ESEHFLRAGKWKKWSYDSFVGPDIHGATLGIIGMGRIGQAIARRSLGFDMQVLYHNRSRL Sbjct: 121 ESEHFLRAGKWKKWSYDSFVGPDIHGATLGIIGMGRIGQAIARRSLGFDMQVLYHNRSRL 180 Query: 181 APELEARANNARYVSKEELLQQADHVVLVLPYSKDTHHTIAAPELALMKPTATLTNIARG 240 APELEARAN+ARYVSKEELL+QADHV+LVLPYSKDTHHTIAA ELALMKPTATLTNIARG Sbjct: 181 APELEARANHARYVSKEELLRQADHVILVLPYSKDTHHTIAAAELALMKPTATLTNIARG 240 Query: 241 GIVDDAALIAALREGRIAAAGVDVFENEPSFKPEFLDLSNVVLTPHIASASTPTRLAMAN 300 GIVDDAALIAALREGRIAAAGVDVFENEP+FKPEFLDLSNVVLTPHIASASTPTRLAMAN Sbjct: 241 GIVDDAALIAALREGRIAAAGVDVFENEPAFKPEFLDLSNVVLTPHIASASTPTRLAMAN 300 Query: 301 CAAGNLIAALSGQRPPNLLNPDV 323 CAA NLIAALSGQRPPNLLNPDV Sbjct: 301 CAADNLIAALSGQRPPNLLNPDV 323 >gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 249 bits (635), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 148/318 (46%), Positives = 197/318 (61%), Gaps = 11/318 (3%) Query: 1 MKPKIFVARAVFPEVLARLEQHFEV---ESNQDDRIYSADELLAKANGKDGLFTTPSEPV 57 MKP + V + + ++ RL+ F++ + DD LA A+G G S P+ Sbjct: 1 MKPHVVVYKKLPEHLVQRLQAEFDLTLFDRITDDNRADFIAALATAHGMIG----ASLPI 56 Query: 58 TAALFAANPQLKAVCNMAVGYNNIDVAAATKAGVMATNTPDVLNETTADFGWALMMAAAR 117 T A+ A PQLK V ++VG +N D+ + G+M +TP VL E TAD +AL++A+AR Sbjct: 57 TNAMLEAAPQLKIVSTISVGVDNFDLDYFRQRGLMLAHTPGVLTEATADTIFALILASAR 116 Query: 118 RITESEHFLRAGKWKKWSYDSFVGPDIHGATLGIIGMGRIGQAIARRS-LGFDMQVLYHN 176 R+ E +++AG+WK ++ G ++HG TLG+IGMGRIG A+ARR+ GF M +LYHN Sbjct: 117 RVVELAEYVKAGRWKGSIGEAQFGVNVHGKTLGLIGMGRIGSAVARRAHHGFGMPILYHN 176 Query: 177 RSRLAPELEARANNARYVSKEELLQQADHVVLVLPYSKDTHHTIAAPELALMKPTATLTN 236 R R PE E R ARYVSKE+LL QAD V ++LP + T APE ALMK +A N Sbjct: 177 R-RPDPEAE-RELGARYVSKEDLLAQADFVCVMLPLTPQTERMFGAPEFALMKRSAIFIN 234 Query: 237 IARGGIVDDAALIAALREGRIAAAGVDVFENEP-SFKPEFLDLSNVVLTPHIASASTPTR 295 +RG IVD+AALIAAL++ I AG+DVFE EP + L L NVV PHI SA+ TR Sbjct: 235 ASRGRIVDEAALIAALQDKTIHGAGLDVFEVEPLPLQSPLLKLPNVVALPHIGSATHETR 294 Query: 296 LAMANCAAGNLIAALSGQ 313 LAMA A NLIA L G+ Sbjct: 295 LAMAELAVTNLIAGLRGE 312 >gi|300313156|ref|YP_003777248.1| D-2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 316 Score = 213 bits (541), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 128/312 (41%), Positives = 177/312 (56%), Gaps = 11/312 (3%) Query: 2 KPKIFVARAVFPEVLARLEQHFEVESNQDDRIYSADELLAKANGKDGLFTTPSEPVTAAL 61 +P + + + +L L+QH+ N + +S EL A A G+ V+ Sbjct: 6 RPHVLIVARLPQHLLDLLQQHYTCH-NLILQPHSEAELAAIAPQIRGIAANGEAKVSREF 64 Query: 62 FAANPQLKAVCNMAVGYNNIDVAAATKAGVMATNTPDVLNETTADFGWALMMAAARRITE 121 A P L+ V VGY+ +DV AA + G+ T+TPDVLN+ AD ALM+A AR + Sbjct: 65 MARFPALEIVSVFGVGYDGVDVPAARERGIHVTHTPDVLNDDVADLAMALMLATARNVVR 124 Query: 122 SEHFLRAGKWKKWSYDSFVGPDIHGATLGIIGMGRIGQAIARRSLGFDMQVLYHNRSRLA 181 ++ F R+G+WKK + + GA LGI+G+GRIGQAIA+R+ FDMQ+ YHNRSR Sbjct: 125 ADRFARSGEWKKGPFP--FTTKVSGARLGIVGLGRIGQAIAKRAAAFDMQISYHNRSR-- 180 Query: 182 PELEARANNARYV-SKEELLQQADHVVLVLPYSKDTHHTIAAPELALMKPTATLTNIARG 240 + YV S L ++ D +V++ P T + A L + P L N+ARG Sbjct: 181 -----KDVPYTYVDSITALAREVDFLVMITPGGAGTRALVNAEVLEALGPKGFLINVARG 235 Query: 241 GIVDDAALIAALREGRIAAAGVDVFENEPSFKPEFLDLSNVVLTPHIASASTPTRLAMAN 300 +VD+AALIAAL+ G IA AG+DVF +EP+ E L NVVLTPH+AS + TR AMA+ Sbjct: 236 SVVDEAALIAALKTGVIAGAGLDVFADEPNVPAELAALDNVVLTPHMASGTLVTRTAMAD 295 Query: 301 CAAGNLIAALSG 312 A NL A SG Sbjct: 296 LAFNNLQAHFSG 307 >gi|300311988|ref|YP_003776080.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 310 Score = 149 bits (375), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 99/292 (33%), Positives = 142/292 (48%), Gaps = 11/292 (3%) Query: 1 MKPKIFVARAVFPEVLARLEQHFEVESNQDDRIYSADELLAKANGKDGL-FTTPSEPVTA 59 MKP + V + E A + H+++ D + DE++A K + T S +TA Sbjct: 1 MKPPLLVLIHLSEESTATIAAHYDILYAPDRQ--RRDEMIAGPARKVAVVLTNGSTGLTA 58 Query: 60 ALFAANPQLKAVCNMAVGYNNIDVAAATKAGVMATNTPDVLNETTADFGWALMMAAARRI 119 A P L+ VC + G+ N+DVA A G+ + AD L++A R I Sbjct: 59 EEMRALPHLQLVCTLGAGFENVDVAHAEAHGIEIATGAGTNEDCVADHALGLLLAILRNI 118 Query: 120 TESEHFLRAGKWKKWSYDSFVGPDIHGATLGIIGMGRIGQAIARRSLGFDMQVLYHNRSR 179 + + R G W++ + P + G +GI+GMG IG+ IARR+ FD ++ Y NR + Sbjct: 119 PVLDRYTRDGGWRE---TIPLQPQLAGKRVGIVGMGNIGKKIARRAAAFDAEIAYCNRKK 175 Query: 180 LAPELEARANNARYVSKEELLQQADHVVLVLPYSKDTHHTIAAPELALMKPTATLTNIAR 239 + + +L AD +++ P T H I A L + P L NI R Sbjct: 176 -----RDDVDYHYFPDVAQLAGWADCLIVAAPGGAQTRHLINARVLEELGPQGYLVNIGR 230 Query: 240 GGIVDDAALIAALREGRIAAAGVDVFENEPSFKPEFLDLSNVVLTPHIASAS 291 G IVD AL AAL GR+A AG+DV+E EP + L NVVLTPHIA S Sbjct: 231 GSIVDTDALGAALSSGRLAGAGLDVYEGEPQPPAALIALPNVVLTPHIAGWS 282 >gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 308 Score = 144 bits (364), Expect = 8e-36, Method: Compositional matrix adjust. Identities = 89/256 (34%), Positives = 133/256 (51%), Gaps = 9/256 (3%) Query: 57 VTAALFAANPQLKAVCNMAVGYNNIDVAAATKAGVMATNTPDVLNETTADFGWALMMAAA 116 V+A + A P L+ + G ++ID AA + G+ A+ WAL+MA A Sbjct: 60 VSARVMDAAPILRVISKHGTGIDSIDSQAAQQRGIAVKAAAGANAPAVAEHTWALIMACA 119 Query: 117 RRITESEHFLRAGKWKKWSYDSFVGPDIHGATLGIIGMGRIGQAIARRSLGFDMQVLYHN 176 + + + +R G W K ++ S ++ G TLG++G+G IG+ +A + M VL H+ Sbjct: 120 KNVVGLDQRMREGHWDKSTHKSL---ELQGRTLGLVGLGAIGRRVAAVAAALGMPVLAHD 176 Query: 177 RSRLAPELEARANNARYVSKEELLQQADHVVLVLPYSKDTHHTIAAPELALMKPTATLTN 236 P + + V + L Q+D V L P + + H I A LA MK A L N Sbjct: 177 -----PYAKEAPQGVQLVDLDTLFAQSDVVSLHCPLTAENKHMINAQSLARMKDGAILVN 231 Query: 237 IARGGIVDDAALIAALREGRIAAAGVDVFENEPSFKPEFLD-LSNVVLTPHIASASTPTR 295 ARGG++D+ ALIAAL G++ AAG+D FE EP P L + N VL+PHI ++ Sbjct: 232 TARGGLIDEQALIAALDSGKLRAAGLDSFEKEPFTAPHPLQRVGNAVLSPHIGGVTSDAY 291 Query: 296 LAMANCAAGNLIAALS 311 +AM AA N++A L Sbjct: 292 IAMGTGAASNVLAVLG 307 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,039,866 Number of extensions: 43654 Number of successful extensions: 181 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 161 Number of HSP's successfully gapped: 11 Length of query: 326 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 241 Effective length of database: 1,217,395 Effective search space: 293392195 Effective search space used: 293392195 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)