BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0033.p (327 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporte... 615 e-177 gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate A... 288 3e-79 gi|300310915|ref|YP_003775007.1| alkanesulfonates ABC transporte... 283 1e-77 gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate A... 252 2e-68 gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate A... 140 1e-34 >gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 327 Score = 615 bits (1585), Expect = e-177, Method: Compositional matrix adjust. Identities = 305/327 (93%), Positives = 319/327 (97%) Query: 1 VSANKNKTIHFPRRRSLQRLSAIALGALAFSLGTAPVQAQETKLLRIGYQKAANTLVLLK 60 +SANKNKT+H RRR++QRLSAIALGAL +LG +PVQAQETKLLRIGYQKAANTLVLLK Sbjct: 1 MSANKNKTVHQARRRAIQRLSAIALGALTIALGASPVQAQETKLLRIGYQKAANTLVLLK 60 Query: 61 AHGALEKRLQPLGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFVYTA 120 AHG LEKRLQPLGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFVYTA Sbjct: 61 AHGTLEKRLQPLGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFVYTA 120 Query: 121 YEIPTPEAEGLLVPKDSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLQYSDIQPVY 180 YEIPTP+AEGLLV K+SPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLQYSDIQPVY Sbjct: 121 YEIPTPDAEGLLVQKNSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLQYSDIQPVY 180 Query: 181 LAPADARAAFERGAVDAWAIWDPFQAAAEKQVGARRLASGVGAVSHHQFFLSARPFAAKN 240 LAPADARAAFERGAVDAWAIWDPFQAAAEKQVGARRLA+GVGAV+HHQFFLSARPFAAKN Sbjct: 181 LAPADARAAFERGAVDAWAIWDPFQAAAEKQVGARRLATGVGAVNHHQFFLSARPFAAKN 240 Query: 241 PQVEKILLEEITREGEWVRTHTAEAAAQLAPIQGLDADIIQTGLKRYAHVYKPVDAQVLA 300 PQVEKILLEEI+REGEWVRTHTA+AAAQLAPIQGLDADII+TGLKRYAHVYKPVDAQVLA Sbjct: 241 PQVEKILLEEISREGEWVRTHTAQAAAQLAPIQGLDADIIETGLKRYAHVYKPVDAQVLA 300 Query: 301 EQQKIADAFFELKLIPKQLKVSDAVLK 327 EQQKIADAF+ELKLIPK LKVSDAVLK Sbjct: 301 EQQKIADAFYELKLIPKPLKVSDAVLK 327 >gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 288 bits (738), Expect = 3e-79, Method: Compositional matrix adjust. Identities = 146/315 (46%), Positives = 207/315 (65%), Gaps = 4/315 (1%) Query: 13 RRRSLQRLSAIALGALAFSLGTAPVQAQETKLLRIGYQKAANTLVLLKAHGALEKRLQPL 72 RRR L +A+ LG+ +L A V AQ K +RIGYQK++ L +LKA+G LEK L P Sbjct: 10 RRRLLHAATALGLGS---ALAPASVFAQGNKAIRIGYQKSSTLLTILKANGTLEKLLAPT 66 Query: 73 GVEVKWAEFAAGPQLLEALNVGSVDFGY-VGEAPPVFAQAAGADFVYTAYEIPTPEAEGL 131 G ++ W EFA+G LLEALNVG VD V + PVFAQAAGA Y A E P+P A+ + Sbjct: 67 GAKISWHEFASGLPLLEALNVGGVDLSADVADTVPVFAQAAGAKLAYVAQESPSPAAQAI 126 Query: 132 LVPKDSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLQYSDIQPVYLAPADARAAFE 191 +V DS ++ ++LKGKKIA K + VH+L++A L+KAGL++SD+ YL+PAD RAAFE Sbjct: 127 VVRADSVLRKASDLKGKKIAVTKAAGVHYLLIATLEKAGLKFSDVDAAYLSPADGRAAFE 186 Query: 192 RGAVDAWAIWDPFQAAAEKQVGARRLASGVGAVSHHQFFLSARPFAAKNPQVEKILLEEI 251 RG+VDAW WDPF A ++Q R L+ G G + +++L++ FA P+V +++ +E+ Sbjct: 187 RGSVDAWVTWDPFLAGVQRQSQVRILSDGRGVADYQRYYLASASFAQARPEVLRVVFDEL 246 Query: 252 TREGEWVRTHTAEAAAQLAPIQGLDADIIQTGLKRYAHVYKPVDAQVLAEQQKIADAFFE 311 + G WV+ H EAAA LAP+ GLDAD I+ R ++ + V + L EQQ+IAD FF Sbjct: 247 QKTGHWVKQHPKEAAAFLAPLWGLDADTIELANSRRSYEVRAVRPEALGEQQRIADTFFA 306 Query: 312 LKLIPKQLKVSDAVL 326 L+P+++ +D + Sbjct: 307 AGLLPRKINTNDVAI 321 >gi|300310915|ref|YP_003775007.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 336 Score = 283 bits (724), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 155/327 (47%), Positives = 210/327 (64%), Gaps = 7/327 (2%) Query: 3 ANKNKTIHF----PRRRSLQRLSAIALGALAFSLGT-APVQAQETKLLRIGYQKAANTLV 57 A +NK I PRRR L + A A A V A + LR+GYQK A L Sbjct: 6 AQRNKAIQTAVRSPRRRRLIAGAVTLGAAAAAGASLPAWVHAAAPRQLRVGYQKGA--LS 63 Query: 58 LLKAHGALEKRLQPLGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFV 117 L++AHG LE+RL PLG VKW EF+AGP LEAL GS+DFG VGEAPP+FAQAAG V Sbjct: 64 LIRAHGVLEQRLAPLGATVKWVEFSAGPVQLEALGAGSIDFGDVGEAPPIFAQAAGTPLV 123 Query: 118 YTAYEIPTPEAEGLLVPKDSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLQYSDIQ 177 Y +P P E +LV DS ++ +LKGK+IA NKGS+VH+ +V LL+K GL+Y D+ Sbjct: 124 YVGATVPRPYVEAVLVKDDSELQRPQQLKGKRIALNKGSNVHYFLVKLLEKHGLKYGDVT 183 Query: 178 PVYLAPADARAAFERGAVDAWAIWDPFQAAAEKQVGARRLASGVGAVSHHQFFLSARPFA 237 PV+LAPADARAAFERGAVDAWAIW+P+ AAA++ + AR LA G V + ++ S R +A Sbjct: 184 PVWLAPADARAAFERGAVDAWAIWEPYLAAAQQGLKARILADADGVVKNRAYYFSTRDYA 243 Query: 238 AKNPQVEKILLEEITREGEWVRTHTAEAAAQLAPIQGLDADIIQTGLKRYAHVYKPVDAQ 297 + + +I LEE+ + W + E A++LA I GL ++ ++RY + P+ Sbjct: 244 RDHADLLRIALEELGKIDAWAQARPDEYASELAGILGLPRSVLDVVVRRYHYGTLPITRA 303 Query: 298 VLAEQQKIADAFFELKLIPKQLKVSDA 324 +L EQQ+IAD F +L+LIP+++ + +A Sbjct: 304 ILDEQQQIADTFLDLRLIPRKISLLEA 330 >gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 323 Score = 252 bits (644), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 139/317 (43%), Positives = 194/317 (61%), Gaps = 4/317 (1%) Query: 10 HFPRRRSLQRLSAIALGALAFSLGTAPVQAQETKLLRIGYQKAANTLVLLKAHGALEKRL 69 H PR + L +A GALA GT V+A K+LRIGYQK NTL +LK G LE+ L Sbjct: 4 HQPRHKRRLLLQGLAAGALAS--GTPLVRAAADKVLRIGYQKF-NTLNILKGSGKLEQAL 60 Query: 70 QPLGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFVYTAYEIPTPEAE 129 QP G VKW EFAAGPQL EALN ++D G+ + P VFA A+G + VY E P P+ Sbjct: 61 QPQGWSVKWFEFAAGPQLTEALNANAIDLGHAADTPSVFANASGVNAVYLGAEQPYPKGI 120 Query: 130 GLLVPKDSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLQYSDIQPVYLA-PADARA 188 G+ V ++SPI+SVA+LKGKK+A +G +V +L++ L++ GL Y DI+PVY+ AD RA Sbjct: 121 GIFVAQESPIRSVADLKGKKVATGRGWNVQFLLLKALEEVGLSYRDIEPVYVTNAADVRA 180 Query: 189 AFERGAVDAWAIWDPFQAAAEKQVGARRLASGVGAVSHHQFFLSARPFAAKNPQVEKILL 248 AF+ G VDA +WDPF A E R L G G ++ F L+ F ++ + + + Sbjct: 181 AFQSGNVDAATLWDPFLAGQELATRPRILRDGTGLSNNRTFHLAVPSFVDRHKDIVRTVF 240 Query: 249 EEITREGEWVRTHTAEAAAQLAPIQGLDADIIQTGLKRYAHVYKPVDAQVLAEQQKIADA 308 E+ + +W ++H E A LAP G+D +++ +R + VDA ++AEQQ+IADA Sbjct: 241 AELKKTNQWTQSHPQETAELLAPQLGVDPKVLRLATERRNYTTVAVDAGIVAEQQQIADA 300 Query: 309 FFELKLIPKQLKVSDAV 325 F +L LI ++V D V Sbjct: 301 FLKLGLIKNPVRVQDKV 317 >gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 140 bits (354), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 103/276 (37%), Positives = 146/276 (52%), Gaps = 7/276 (2%) Query: 14 RRSLQRLSAIALGALAFSLGTAPV---QAQETKLLRIGYQKAANTLVLLKAHGALEKRLQ 70 RR RL+ ALG A LG P+ AQ K++R+ Y + ++L+ G LE+ L+ Sbjct: 3 RRDFLRLAPAALGVTA--LGGIPLLSSAAQPPKVIRLDYAYYSPPSLVLRKFGWLEEDLK 60 Query: 71 PLGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFVYTAYEIPTPEAEG 130 G EVKW + LE LN SVDFG + A+A G + V Y PE Sbjct: 61 AQGTEVKWVLSQGSNRALEYLNSDSVDFGSTAGLAALLARANG-NPVKAVYIYSRPEWTA 119 Query: 131 LLVPKDSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLQYSDIQPVYLAPADARAAF 190 L V K+SP+KS+ ELKGKKIA KG+D + ++ L + GL+ SD++ V+L AD RAA Sbjct: 120 LAVGKESPVKSINELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQHADGRAAL 179 Query: 191 ERGAVDAWAIWDPFQAAAEKQVGARRLASGVGAVSHHQFFLSARPFAAKNPQVEKILLEE 250 E+G V AWA DP AA+E + G+R + V + + F + FAA++P K ++ Sbjct: 180 EQGRVAAWAGLDPHLAASELEAGSRLIYRNVN-FNTYGFLNVSDSFAARHPDQVKRVIAA 238 Query: 251 ITREGEWVRTHTAEAAAQLAPIQGLDADIIQTGLKR 286 R W+ H E A L+ L A + + LKR Sbjct: 239 YERARSWIIAHPDETVALLSEESKLSAPVARLQLKR 274 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,013,288 Number of extensions: 43237 Number of successful extensions: 199 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 192 Number of HSP's successfully gapped: 8 Length of query: 327 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 242 Effective length of database: 1,217,395 Effective search space: 294609590 Effective search space used: 294609590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)