BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0032.p (370 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310978|ref|YP_003775070.1| alkanesulfonate monooxygenase [... 660 0.0 gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [... 482 e-137 gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-... 119 3e-28 gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-... 60 3e-10 >gi|300310978|ref|YP_003775070.1| alkanesulfonate monooxygenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 660 bits (1704), Expect = 0.0, Method: Compositional matrix adjust. Identities = 336/358 (93%), Positives = 348/358 (97%) Query: 1 MNVFWFIPTHGDSRYLGTSEGARPVTHDYMKQIAVAADTLGYDGVLLPTGRSCEDAWVAA 60 MNVFWFIPTHGDSRYLGTSEGAR V+HDYMKQ+AVAADTLGYDGVLLPTGRSCEDAWVAA Sbjct: 1 MNVFWFIPTHGDSRYLGTSEGARAVSHDYMKQVAVAADTLGYDGVLLPTGRSCEDAWVAA 60 Query: 61 ASLIDATQRLKFLVAVRPGLTAPTLSARMAATFDRLSNGRLLINVVTGGDPVEQESDGIF 120 ASLIDAT+RLKFLVAVRPGL APTL+ARMAATFDRLS GRLLINVVTGGDPVEQESDG+F Sbjct: 61 ASLIDATRRLKFLVAVRPGLMAPTLAARMAATFDRLSGGRLLINVVTGGDPVEQESDGVF 120 Query: 121 GSHAERYEISDEFLKIWREVLAKTHTGEETNFEGKHLSVKGAKALYPPVQTPYPPLYFGG 180 GSHAERYEISDEFLKIWR+VL KT +GE T+F+GKHLSVKGAKALYPPVQ PYPPLYFGG Sbjct: 121 GSHAERYEISDEFLKIWRDVLTKTRSGEPTHFQGKHLSVKGAKALYPPVQQPYPPLYFGG 180 Query: 181 SSDEAIDLAAEQVDVYLTWGEPPAAVAEKIARVRERAAKAGRTLRFGIRLHVIVRDTNEA 240 SSDEAIDLAAEQVDVYLTWGEPPAAVAEKIARVRERAAKAGRTLRFGIRLHVIVR+TNEA Sbjct: 181 SSDEAIDLAAEQVDVYLTWGEPPAAVAEKIARVRERAAKAGRTLRFGIRLHVIVRETNEA 240 Query: 241 AWRAADELISRLDDETIAKAQANFARMDSEGQRRMAALHGGRRDKLEVSPNLWAGVGLVR 300 AWRAADELISRLDDETIAKAQANFARMDSEGQRRMAALHGGRRDKLEVSPNLWAGVGLVR Sbjct: 241 AWRAADELISRLDDETIAKAQANFARMDSEGQRRMAALHGGRRDKLEVSPNLWAGVGLVR 300 Query: 301 GGAGTALVGDGPTVAARIKEYADLGIDTFILSGYPHLEESYRFAELVFPLLPRAQREG 358 GGAGTALVGDGPTVA R++EYA LGIDTFILSGYPHLEESYRFAELVFPLLPR QREG Sbjct: 301 GGAGTALVGDGPTVAERLQEYAALGIDTFILSGYPHLEESYRFAELVFPLLPRGQREG 358 >gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [Herbaspirillum seropedicae SmR1] Length = 375 Score = 482 bits (1241), Expect = e-137, Method: Compositional matrix adjust. Identities = 232/351 (66%), Positives = 278/351 (79%), Gaps = 4/351 (1%) Query: 1 MNVFWFIPTHGDSRYLGTSEGARPVTHDYMKQIAVAADTLGYDGVLLPTGRSCEDAWVAA 60 + +FWF+PT GD+RYLG S+ R T +YM+QIAV ++TLGYDG+L+PTG SC D WV A Sbjct: 3 LELFWFLPTSGDTRYLGKSDFGRAPTLEYMRQIAVTSETLGYDGLLIPTGASCLDPWVVA 62 Query: 61 ASLIDATQRLKFLVAVRPGLTAPTLSARMAATFDRLSNGRLLINVVTGGDPVEQESDGIF 120 ASL+ TQR+K LVA+R L P SAR AAT D+ NGRLL+NVV GGD E E+DG+F Sbjct: 63 ASLVPVTQRIKLLVALRTSLGGPGPSARQAATLDQALNGRLLLNVVPGGDATELEADGVF 122 Query: 121 GSHAERYEISDEFLKIWREVLAKTHTGEETNFEGKHLSVKGAKALYPPVQTPYPPLYFGG 180 H ERYE +DE+L IWR++L GE N+EGKH ++ + +P VQ PYPPLYFGG Sbjct: 123 LKHDERYEAADEYLTIWRKLL----QGETVNYEGKHFRIRNGQLFHPAVQQPYPPLYFGG 178 Query: 181 SSDEAIDLAAEQVDVYLTWGEPPAAVAEKIARVRERAAKAGRTLRFGIRLHVIVRDTNEA 240 SS A DLAA+ VD YLTWGEPPAAV +KI VRERAA+ GRT+RFG+RLH IVR+T Sbjct: 179 SSPAAHDLAAKHVDAYLTWGEPPAAVRQKIDDVRERAARQGRTVRFGVRLHAIVRETTAE 238 Query: 241 AWRAADELISRLDDETIAKAQANFARMDSEGQRRMAALHGGRRDKLEVSPNLWAGVGLVR 300 AW AA+ LIS+L DE IA+AQ N+ARMDS GQ+RMAALHGGRRDKLE++PNLWAGVGLVR Sbjct: 239 AWTAAERLISKLTDEDIARAQQNYARMDSVGQQRMAALHGGRRDKLEIAPNLWAGVGLVR 298 Query: 301 GGAGTALVGDGPTVAARIKEYADLGIDTFILSGYPHLEESYRFAELVFPLL 351 GGAGT+LVGD TVA R++EYADLG+DTF+LSGYPHLEE+ RFAELVFPLL Sbjct: 299 GGAGTSLVGDATTVAERLQEYADLGVDTFVLSGYPHLEEAIRFAELVFPLL 349 >gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 119 bits (299), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 101/338 (29%), Positives = 158/338 (46%), Gaps = 34/338 (10%) Query: 28 DYMKQIAVAADTLGYDGVL----LPTGRSCE---DAWVAAASLIDATQRLKFLVAVRPGL 80 +Y +++AV A+ G++ L G E ++ + +L+ AT +LK L A+ PG Sbjct: 44 EYNQRLAVTAEKAGFEYALSQIRFTAGYGAEYQHESVSFSQALLHATTKLKVLAAILPGP 103 Query: 81 TAPTLSARMAATFDRLSNGRLLINVVTGGDPVEQESDG-IFGSHAERYEISDEFLKIWRE 139 P + A+ AT D +SNGR+ INVV+G E ++ G + H ERY S+EF++ Sbjct: 104 WHPAVVAKQIATIDHISNGRIAINVVSGWFKGEFQAIGEPWLEHDERYRRSNEFIR---- 159 Query: 140 VLAKTHTGEETNFEGKHLSVKGAKALYPPVQTPYPPLYFGGSSDEAIDLAAEQVDVYLTW 199 L T ++ F+G PVQ P+P ++ GGSS A D AA D Y T Sbjct: 160 ALKGIWTQDDFTFKGDFYRFNNYTLSPKPVQKPHPEIFQGGSSRAARDNAASVSDWYFTN 219 Query: 200 GEPPAAVAEKIARVRERAAKAGRTLRFGIRLHVIVRDTNEAAWRAADELISRLDDETI-- 257 G + +I ++ +AA+ G +R G+ VI RDT E A ++I E + Sbjct: 220 GNTLEGLKAQIDDIQAKAAQNGHKVRIGVNAFVIARDTEEEAQAVLQDIIDHAHVEAVHA 279 Query: 258 ---AKAQANFARMDSEGQRRMAALHGGRRDKLEVSPNLWAGVGLVRGGAGTALVGDGPTV 314 A QA A + EG + D ++ + G T L+G + Sbjct: 280 FGDAVKQAGKASPEGEGNWAKSTF----EDLVQYN-----------DGFKTNLIGTPQQI 324 Query: 315 AARIKEYADLGIDTFILSGYPH-LEESYRFAELVFPLL 351 A RI +G+D +L+G+ H +EE F + V PL+ Sbjct: 325 AERIVALKAIGVD-LVLTGFLHFIEEVEYFGQKVLPLV 361 >gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 60.1 bits (144), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 87/341 (25%), Positives = 134/341 (39%), Gaps = 50/341 (14%) Query: 53 CEDAWVAAASLIDATQRLKFLVAVRPGLTAPTLSARMAATFDRLSNGRLLINVVTGGDPV 112 D + ++ T+ L F V AP L AR +T D LS GR+ N+VTG Sbjct: 83 VNDPLMLVPAMAAVTEHLGFGVTANLTYEAPFLFARRMSTLDHLSRGRVGWNIVTGYLDS 142 Query: 113 EQESDGIFGS--HAERYEISDEFL----KIW--------------REVLAK-------TH 145 + G+ H +RY+ +DEF+ K+W R V A H Sbjct: 143 AARAMGLHEQLEHDQRYDRADEFMEVVYKLWEGSWEDGAVLRDRARRVYADPAKVHKIAH 202 Query: 146 TGEETNFEGKHLSVKGAKALYPPVQTPYPPLYFGGSSDEAIDLAAEQVDVYLTWGEPPAA 205 G+ + EG HLS P + P LY GSS A + + A Sbjct: 203 HGDYYHVEGIHLS--------EPSRQRTPVLYQAGSSGRGQAFGARHAECVFISSQSKDA 254 Query: 206 VAEKIARVRERAAKAGR---TLRFGIRLHVIVRDTNEAAWRAADELISRLDDET-IAKAQ 261 + + +R+ A + GR ++ + L V+ T + A E E +A Sbjct: 255 TRKLVQSIRDGAVREGRDPDAIKIFMGLTVVTGRTEQQAQERYREYARYASPEAGLAHYS 314 Query: 262 A----NFARMDSEGQRRMAALHGGRR-DKLEVSPNL-WAGVGLVR----GGAGTALVGDG 311 A +F++ + + R A + K+ P L W L++ GG ++VG Sbjct: 315 AGTGIDFSQYELDEPIRPAKTNAIESLTKVVTQPELNWTKRKLLQQLELGGRYASIVGAP 374 Query: 312 PTVAARIKEY-ADLGIDTFILSGYPHLEESYRFAELVFPLL 351 VA ++ + AD GID + L+ E F ELV P+L Sbjct: 375 DRVADELESWVADTGIDGYNLTRTVTPESFEDFVELVIPVL 415 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,280,759 Number of extensions: 57675 Number of successful extensions: 204 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 203 Number of HSP's successfully gapped: 4 Length of query: 370 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 283 Effective length of database: 1,207,925 Effective search space: 341842775 Effective search space used: 341842775 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)