BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0026.p (194 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310983|ref|YP_003775075.1| alpha/beta hydrolase fold ester... 330 3e-92 >gi|300310983|ref|YP_003775075.1| alpha/beta hydrolase fold esterase [Herbaspirillum seropedicae SmR1] Length = 192 Score = 330 bits (847), Expect = 3e-92, Method: Compositional matrix adjust. Identities = 165/194 (85%), Positives = 178/194 (91%), Gaps = 2/194 (1%) Query: 1 MSLTSPSSSTTPARLADFRVLVAPGLSDSGPEHWQSRWQRLYPAFERVKQDDWETPDLPR 60 MSL+S +SS PARLADFRVLVAPGLSDSG EHWQSRWQRLYP FERV+QDDWE+PDLPR Sbjct: 1 MSLSSQTSSA-PARLADFRVLVAPGLSDSGAEHWQSRWQRLYPGFERVRQDDWESPDLPR 59 Query: 61 WSARLGEVLARDGRPTVIVAHSFGCLTTVHRTAALQAASEGSAHPIVAALLVAPADPVKF 120 WS RLGEVLA+DGRPTVIVAHSFGCL TVHR AALQA GSA+PIVAALLVAPADPVKF Sbjct: 60 WSQRLGEVLAQDGRPTVIVAHSFGCLATVHRAAALQAEG-GSAYPIVAALLVAPADPVKF 118 Query: 121 SLSAAVRHKLPYPALVVGSDNDPWMSAERARHWAEAWGAQFRSVGARGHINSESGLGDWT 180 +SAAVRHKLP+ A V+GSDNDPWMSAERARHWAEAWGA FRSVGA GHIN+ESGLGDWT Sbjct: 119 GVSAAVRHKLPFAARVIGSDNDPWMSAERARHWAEAWGADFRSVGAHGHINAESGLGDWT 178 Query: 181 EGQQWLLELLERIA 194 +GQQWLLELL++IA Sbjct: 179 QGQQWLLELLDQIA 192 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.131 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 607,568 Number of extensions: 23169 Number of successful extensions: 97 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 96 Number of HSP's successfully gapped: 1 Length of query: 194 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 114 Effective length of database: 1,241,070 Effective search space: 141481980 Effective search space used: 141481980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)