BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0014.p (299 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC t... 573 e-165 gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC t... 168 4e-43 gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC t... 137 8e-34 gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC t... 122 4e-29 gi|300312236|ref|YP_003776328.1| amino acid ABC transporter peri... 77 2e-15 >gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 373 Score = 573 bits (1477), Expect = e-165, Method: Compositional matrix adjust. Identities = 289/299 (96%), Positives = 295/299 (98%) Query: 1 MDDACEPKQAVAVANKIVSQGIKYVIGHVCSGSTIPASDIYENEGVVMITPSATAPQLTE 60 MDDACEPKQAVAVANKIVSQGIKYVIGHVCSGSTIPASDIYENEGVVMITPSATAPQLTE Sbjct: 75 MDDACEPKQAVAVANKIVSQGIKYVIGHVCSGSTIPASDIYENEGVVMITPSATAPQLTE 134 Query: 61 AKPHKFIFRTIGRDDQQGPAAARYVIEKLKPKKVAVLHDKQSYGQGVASSVKSALEAAKV 120 AKPHKFIFRTIGRDDQQGPAAARYVIEKLKPKKVAVLHDKQSYGQGVASSVK+AL+AAKV Sbjct: 135 AKPHKFIFRTIGRDDQQGPAAARYVIEKLKPKKVAVLHDKQSYGQGVASSVKAALDAAKV 194 Query: 121 PVVVFEGINAGDSDYSAIVTKLKSQGVDFVYFGGYHPEMGLIMRQAREQGVKAVFMGPEG 180 PVVVFEGINAGDSDYSAIVTKLKSQGVDFVYFGGYHPEMGLIMRQAREQG+KAVFMGPEG Sbjct: 195 PVVVFEGINAGDSDYSAIVTKLKSQGVDFVYFGGYHPEMGLIMRQAREQGIKAVFMGPEG 254 Query: 181 VGNKDITAIAGPASEGMLVTLPADFAADPANAALVKAFAAAKRDANGPFQMPAYSAVKII 240 VGNKDITAIAGPASEGMLVTLPADFAADPANAALVKAFAAAKRD NGPFQMPAYS VKII Sbjct: 255 VGNKDITAIAGPASEGMLVTLPADFAADPANAALVKAFAAAKRDPNGPFQMPAYSGVKII 314 Query: 241 ADAITGAKSTDPDKVAAYIHGNSFKTPIGNVEYDKKGDLKAFKFVVFTWHKDATKTEAK 299 ADAI GAKSTDP+KVAAYIH NSFKTPIGNVEYDKKGDLK+FKFVVFTWHKDA+KTEAK Sbjct: 315 ADAIAGAKSTDPEKVAAYIHANSFKTPIGNVEYDKKGDLKSFKFVVFTWHKDASKTEAK 373 >gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 380 Score = 168 bits (426), Expect = 4e-43, Method: Compositional matrix adjust. Identities = 106/292 (36%), Positives = 156/292 (53%), Gaps = 5/292 (1%) Query: 2 DDACEPKQAVAVANKIVSQGIKYVIGHVCSGSTIPASDIYENEGVVMITPSATAPQLTEA 61 DDA +PKQA AVA K+V Q + VIGH+ SG+TIPAS IY + G+ ++PSAT P+ T+ Sbjct: 78 DDASDPKQATAVATKLVDQKVAAVIGHLNSGTTIPASKIYSDAGIPQVSPSATNPKYTQ- 136 Query: 62 KPHKFIFRTIGRDDQQGPAAARYVIEKLKPKKVAVLHDKQSYGQGVASSVKSALEAAKVP 121 + K FR + D Q G A +Y ++KL K++AV+ D+ +YGQGVA +AA Sbjct: 137 QGFKTAFRVVANDAQLGAALGKYAVQKLGTKQIAVIDDRTAYGQGVADEFAKGAKAAGGT 196 Query: 122 VVVFEGINAGDSDYSAIVTKLKSQGVDFVYFGGYHPEMGLIMRQAREQGVKAVFMGPEGV 181 +V + N +D++AI+T LK + D V+FGG G ++RQ ++ G+ A FMG +G+ Sbjct: 197 IVGTQYTNDKATDFNAILTSLKGKKPDVVFFGGMDAVGGPMLRQMKQLGISAKFMGGDGI 256 Query: 182 GNKDITAIAGPASEGMLVTLPADFAADPANAALVKAFAAAKRDANGP----FQMPAYSAV 237 + +AG V D A + F AA + G AY A Sbjct: 257 CTGSLPGLAGDGLGDDQVICAEAGGVDEAGKKGMDDFRAAYKKKFGIDVVYNAAYAYDAT 316 Query: 238 KIIADAITGAKSTDPDKVAAYIHGNSFKTPIGNVEYDKKGDLKAFKFVVFTW 289 +ADA+ A S DP K + S K G + +D KGD+K ++T+ Sbjct: 317 MTVADAMAKAGSADPKKYLPELAKISHKGVTGVIAFDAKGDIKDGSLTLYTY 368 >gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 378 Score = 137 bits (346), Expect = 8e-34, Method: Compositional matrix adjust. Identities = 94/292 (32%), Positives = 151/292 (51%), Gaps = 8/292 (2%) Query: 2 DDACEPKQAVAVANKIVSQGIKYVIGHVCSGSTIPASDIYENEGVVMITPSATAPQLTEA 61 DD +PK VAVA K+ QG+K ++G SG TIPAS +Y + G+V+ T A+ P++T+ Sbjct: 80 DDQADPKSGVAVAQKLADQGVKAIVGPYNSGVTIPASRVYNDAGIVVAT-VASNPKITQ- 137 Query: 62 KPHKFIFRTIGRDDQQGPAAARYVIEKLKPKKVAVLHDKQSYGQGVASSVKSALEAAKVP 121 + +FR D Q G A Y ++LK K+VAV+ D+ +YGQG+A +A + Sbjct: 138 QGFATLFRVAASDSQLGGKMALYAAKELKFKRVAVIDDRTAYGQGLAQEFIKVAKANGID 197 Query: 122 VVVFEGINAGDSDYSAIVTKLKSQGVDFVYFGGYHPEMGLIMRQAREQGVKAVFMGPEGV 181 VV + N +D++AI+T +K + D V+ GGY P+ G I RQ ++ GV MG +G+ Sbjct: 198 VVSTDFTNDKATDFTAILTSIKGKKPDAVFLGGYAPQGGPIKRQMKQLGVDVPLMGGDGI 257 Query: 182 GNKDITAIAGPASEGMLVTLPADFAADPANAALVKAFA-AAKRDANGP---FQMPAYSAV 237 + ++ + G A + D A K F+ ++ N P + + Y + Sbjct: 258 CSPEMGRLGGDAIGESVYCTQGGTMLDKAKEG--KVFSDEYQKKYNRPAETYAVSFYDGM 315 Query: 238 KIIADAITGAKSTDPDKVAAYIHGNSFKTPIGNVEYDKKGDLKAFKFVVFTW 289 +IA A+ A S DP + + S+K G ++D DLK V+ + Sbjct: 316 MLIAQAMKQANSVDPKQFGPALAKISYKGVAGQYDFDANHDLKQSPVTVYRF 367 >gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 394 Score = 122 bits (305), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 80/287 (27%), Positives = 138/287 (48%), Gaps = 2/287 (0%) Query: 2 DDACEPKQAVAVANKIVSQGIKYVIGHVCSGSTIPASDIYENEGVVMITPSATAPQLTEA 61 DD +P A VA +V Q + V+GH SG+++ A+ +Y GV+ +TPSA + +LT A Sbjct: 90 DDRADPATAEFVARSLVQQQVIAVVGHWTSGTSLAAAPVYHRAGVIQVTPSAMSRRLT-A 148 Query: 62 KPHKFIFRTIGRDDQQGPAAARYVIEKLKPKKVAVLHDKQSYGQGVASSVKSALEAAKVP 121 + IFRTI ++ G AA Y ++KL+ + + + D+ +GQG+A Sbjct: 149 MSYPRIFRTIPNNESLGRLAAGYAVDKLETRTIVTIDDRTPFGQGLAEQFARTASERGAQ 208 Query: 122 VVVFEGINAGDSDYSAIVTKLKSQGVDFVYFGGYHPEMGLIMRQAREQGVKAVFMGPEGV 181 VV ++ SD++A + + + D ++FGG + G++ + R + A M G Sbjct: 209 VVGRYSVSDKTSDFNAALLEARKLQPDLIFFGGLDWQAGVLAKSIRRLKLHARLMASPGT 268 Query: 182 GNKDITAIAGPASEGMLVTLPADFAAD-PANAALVKAFAAAKRDANGPFQMPAYSAVKII 240 AGP + G LV P P + + ++ + + AY A + I Sbjct: 269 LGLPFLMRAGPDANGALVLEPGPPQDKMPGWKSFRQRYSENFDSDMDLYAVFAYEAAQAI 328 Query: 241 ADAITGAKSTDPDKVAAYIHGNSFKTPIGNVEYDKKGDLKAFKFVVF 287 I A S DP+++A +H F+ G V ++++GDL F ++ Sbjct: 329 IQGIRQAGSADPERIAEAMHRLRFEGVSGPVAFNEEGDLLQPSFTMY 375 >gi|300312236|ref|YP_003776328.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 76.6 bits (187), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 72/252 (28%), Positives = 115/252 (45%), Gaps = 9/252 (3%) Query: 2 DDACEPKQAVAVANKIVSQG-IKYVIGHVCSGSTIPASDIYENEGVVMITPSATAPQLTE 60 D+ + ++A+ V K++ Q + V G S S A I VV+ S TA +T Sbjct: 75 DEQGKKEEAINVFKKLIFQDKVLAVFGPTLSNSAFAADPIANAAKVVVFGTSNTADGITA 134 Query: 61 AKPHKFIFRTIGRDDQQGPAAARYVIEKLKPKKVAVLH-DKQSYGQGVASSVKSALEAAK 119 P F FR + P + ++ KKVAV++ + ++ + K+ LE K Sbjct: 135 MGP--FTFRNSVMEADVLPVTVKAAVKHFGIKKVAVIYGNDDAFTKSGYDVFKATLEHQK 192 Query: 120 VPVVVFEGINAGDSDYSAIVTKLKSQGVDFVYFGGYHPEMGLIMRQAREQGVKAVFMGPE 179 +PV E GD D+ A +TK+K+ D + E I+ Q R G+K F+G Sbjct: 193 IPVTTTEAYAKGDVDFKAQLTKIKAGNPDAIVCSCLAEEAANIILQTRALGMKQPFIGGN 252 Query: 180 GVGNKDITAIAGPASEGMLVTLPADFAAD---PANAALVKAFAAAKRDANGPFQMPAYSA 236 G+ + + IA A + L+ P ++A+ PAN A + A+ A F AY A Sbjct: 253 GLNSPKLFEIAKDAGDNTLMGSP--WSAENQTPANKAFITAYKAKFGADPDQFAAQAYDA 310 Query: 237 VKIIADAITGAK 248 + I+ADA+ K Sbjct: 311 MYIVADALKNVK 322 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.132 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 828,078 Number of extensions: 31272 Number of successful extensions: 130 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 123 Number of HSP's successfully gapped: 9 Length of query: 299 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 214 Effective length of database: 1,217,395 Effective search space: 260522530 Effective search space used: 260522530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)