BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0013.p (312 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310992|ref|YP_003775084.1| branched-chain aminoacid ABC tr... 595 e-171 gi|300310521|ref|YP_003774613.1| branched-chain amino acid ABC t... 214 1e-56 gi|300309519|ref|YP_003773611.1| branched-chain amino acid ABC t... 214 1e-56 gi|300312237|ref|YP_003776329.1| amino acid ABC transporter perm... 171 6e-44 gi|300311114|ref|YP_003775206.1| branched-chain amino acid ABC t... 136 2e-33 >gi|300310992|ref|YP_003775084.1| branched-chain aminoacid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 308 Score = 595 bits (1534), Expect = e-171, Method: Compositional matrix adjust. Identities = 306/308 (99%), Positives = 308/308 (100%) Query: 5 MNELLPQLTQQLVNGLSLGAIYALIAIGYTMVYGIIGMINFAHGEIYMIASYVGLVTLTA 64 MNELLPQLTQQLVNGLSLGAIYALIAIGYTMVYGIIGMINFAHGEIYMIASYVGLVTLTA Sbjct: 1 MNELLPQLTQQLVNGLSLGAIYALIAIGYTMVYGIIGMINFAHGEIYMIASYVGLVTLTA 60 Query: 65 IGVQSGYPLPLLLGGALIVSVLVTGLYGWTVERVAYRPLRGGPRLVPLISAIGMSIFLQN 124 IGVQSGYPLPLLLGGALIVSVLVTGLYGWTVERVAYRPLRGGPRLVPLISAIGMSIFLQN Sbjct: 61 IGVQSGYPLPLLLGGALIVSVLVTGLYGWTVERVAYRPLRGGPRLVPLISAIGMSIFLQN 120 Query: 125 YVQIGQGARDMSVPVLISGALEFQMGSDFTVTVPYSRMVIVGVTLVLMVALTLFIARSRM 184 YVQIGQGARDMSVPVLISGALEFQMGSDFTVT+PYSRMVIVGVTLVLMVALTLFIARSRM Sbjct: 121 YVQIGQGARDMSVPVLISGALEFQMGSDFTVTIPYSRMVIVGVTLVLMVALTLFIARSRM 180 Query: 185 GRACRACAEDMGMANLLGIDTNKVISFTFVLGAMLAAVGGVLIALTIGKLNPYIGFIAGI 244 GRACRACAEDMGMANLLGIDTNKVISFTFVLGAMLAAVGGVLIALTIGKLNPYIGFIAGI Sbjct: 181 GRACRACAEDMGMANLLGIDTNKVISFTFVLGAMLAAVGGVLIALTIGKLNPYIGFIAGI 240 Query: 245 KAFTAAVLGGIGSIPGAMLGGVLLGLAETFAAGYLPSEYKDVVSFGLLVLILLFRPTGLL 304 KAFTAAVLGGIGSIPGAMLGGVLLGLAETFAAGYLPSEYKDVVSFGLLVLILLFRPTGLL Sbjct: 241 KAFTAAVLGGIGSIPGAMLGGVLLGLAETFAAGYLPSEYKDVVSFGLLVLILLFRPTGLL 300 Query: 305 GKPDIEKV 312 GKPD+EKV Sbjct: 301 GKPDVEKV 308 >gi|300310521|ref|YP_003774613.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 309 Score = 214 bits (544), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 139/313 (44%), Positives = 203/313 (64%), Gaps = 35/313 (11%) Query: 14 QQLVNGLSLGAIYALIAIGYTMVYGIIGMINFAHGEIYMIASYVGLVTLTAIG-VQSGYP 72 QQ++NGL LG++YALIA+GYTMVYG++ +INFAHG+I M+ + VGL L + V G P Sbjct: 6 QQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDILMVGAMVGLSLLKVVQQVAPGLP 65 Query: 73 ----LPLLLGGALIVSVLVTGLYGWTVERVAYRPLRGGPRLVPLISAIGMSIFLQNYVQI 128 L + + GA+ V ++V+ L +ER+AYRPLR PRL PLI+AIG+SI LQ + Sbjct: 66 GIVQLVIAIVGAIPVCIVVSLL----IERIAYRPLRNAPRLAPLITAIGVSILLQTLAMM 121 Query: 129 GQGARDM-------SVPVLISGALEFQMGSDFTVTVPYSRMVIVGVTLVLMVALTLFIAR 181 G + S PV I+GAL + ++++++ + ++ MV L L + + Sbjct: 122 IWGRSPLPFPQVMPSDPVHIAGAL-----------ISPTQIMLLALAVLAMVGLVLIVEK 170 Query: 182 SRMGRACRACAEDMGMANLLGIDTNKVISFTFVLGAMLAAVGGVLIALTIGKLNPYIGFI 241 ++MGRA RA AE+ +A L+G+D NKVI TF +GA LAA+ GV+ A +GF+ Sbjct: 171 TKMGRAMRATAENPRIAGLMGVDANKVIVVTFAIGAGLAAIAGVMWAANYSTAQFAMGFV 230 Query: 242 AGIKAFTAAVLGGIGSIPGAMLGGVLLGLAETFAAGY--------LPSEYKDVVSFGLLV 293 G+KAF+AAVLGGIG+I GAMLGG+LLGL E+ AGY L S Y+D+ +F +L+ Sbjct: 231 PGLKAFSAAVLGGIGNIYGAMLGGILLGLIESLGAGYIGDLTGNFLGSNYQDIFAFIVLI 290 Query: 294 LILLFRPTGLLGK 306 ++L RP+G++G+ Sbjct: 291 IVLTLRPSGIMGE 303 >gi|300309519|ref|YP_003773611.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 309 Score = 214 bits (544), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 139/313 (44%), Positives = 203/313 (64%), Gaps = 35/313 (11%) Query: 14 QQLVNGLSLGAIYALIAIGYTMVYGIIGMINFAHGEIYMIASYVGLVTLTAIG-VQSGYP 72 QQ++NGL LG++YALIA+GYTMVYG++ +INFAHG+I M+ + VGL L + V G P Sbjct: 6 QQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDILMVGAMVGLSLLKVVQQVAPGLP 65 Query: 73 ----LPLLLGGALIVSVLVTGLYGWTVERVAYRPLRGGPRLVPLISAIGMSIFLQNYVQI 128 L + + GA+ V ++V+ L +ER+AYRPLR PRL PLI+AIG+SI LQ + Sbjct: 66 GIVQLVIAIVGAIPVCIVVSLL----IERIAYRPLRNAPRLAPLITAIGVSILLQTLAMM 121 Query: 129 GQGARDM-------SVPVLISGALEFQMGSDFTVTVPYSRMVIVGVTLVLMVALTLFIAR 181 G + S PV I+GAL + ++++++ + ++ MV L L + + Sbjct: 122 IWGRSPLPFPQVMPSDPVHIAGAL-----------ISPTQIMLLALAVLAMVGLVLIVEK 170 Query: 182 SRMGRACRACAEDMGMANLLGIDTNKVISFTFVLGAMLAAVGGVLIALTIGKLNPYIGFI 241 ++MGRA RA AE+ +A L+G+D NKVI TF +GA LAA+ GV+ A +GF+ Sbjct: 171 TKMGRAMRATAENPRIAGLMGVDANKVIVVTFAIGAGLAAIAGVMWAANYSTAQFAMGFV 230 Query: 242 AGIKAFTAAVLGGIGSIPGAMLGGVLLGLAETFAAGY--------LPSEYKDVVSFGLLV 293 G+KAF+AAVLGGIG+I GAMLGG+LLGL E+ AGY L S Y+D+ +F +L+ Sbjct: 231 PGLKAFSAAVLGGIGNIYGAMLGGILLGLIESLGAGYIGDLTGNFLGSNYQDIFAFIVLI 290 Query: 294 LILLFRPTGLLGK 306 ++L RP+G++G+ Sbjct: 291 IVLTLRPSGIMGE 303 >gi|300312237|ref|YP_003776329.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 293 Score = 171 bits (434), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 110/297 (37%), Positives = 177/297 (59%), Gaps = 13/297 (4%) Query: 12 LTQQLVNGLSLGAIYALIAIGYTMVYGIIGMINFAHGEIYMIASYVGLVTLTAIGVQSGY 71 L QQL+N LSLG++YAL A+G+T+V+G++G+IN +HG I+M+ SY L+ + + Sbjct: 2 LEQQLINALSLGSVYALFALGFTLVFGVLGVINLSHGAIFMLGSYAALLLVEQMA----- 56 Query: 72 PLPLLLGGALIVSVLVTGLYGWTVERVAYRPLR--GGPRLVPLISAIGMSIFLQNYVQIG 129 LPL + AL+++++ TGL G ++ + +PLR P L+P+I+ IG++I + N Q Sbjct: 57 -LPLWV--ALLLAMVATGLLGLIIDVLVLKPLRKRNAPHLIPMIATIGVAIMITNISQGL 113 Query: 130 QGARDMSVPVLISGALEFQMGSDFTVTVPYSRMVIVGVTLVLMVALTLFIARSRMGRACR 189 GA + P G + + S + + ++ I+ + VLMV L + ++++GRA R Sbjct: 114 FGAENKRFP---QGTIPEESVSLGNLHITAVQVAIIVIAFVLMVVLLGVMRKTQLGRALR 170 Query: 190 ACAEDMGMANLLGIDTNKVISFTFVLGAMLAAVGGVLIALTIGKLNPYIGFIAGIKAFTA 249 A AE A LLGI+ + T A L GVL+ ++ ++P++G K Sbjct: 171 AIAESPKAAYLLGINVEGLFLLTSFAAAALGGAAGVLVGVSFNAISPFMGQPMLHKGIAV 230 Query: 250 AVLGGIGSIPGAMLGGVLLGLAETFAAGYLPSEYKDVVSFGLLVLILLFRPTGLLGK 306 +LGG+G I GAM+GG+ LG AE Y+ S+++D V+FGLL LILL +P+G+ GK Sbjct: 231 IILGGMGDIRGAMIGGLFLGFAEVLTVAYISSDFRDAVAFGLLFLILLVKPSGMFGK 287 >gi|300311114|ref|YP_003775206.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 296 Score = 136 bits (343), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 115/305 (37%), Positives = 173/305 (56%), Gaps = 16/305 (5%) Query: 12 LTQQLVNGLSLGAIYALIAIGYTMVYGIIGMINFAHGEIYMIASYVGLVTLTAIGVQSGY 71 L Q + +G++LG IYA+IA GY + + G +NF GE M+ + VGL + I + Sbjct: 4 LLQLVFSGIALGMIYAVIAFGYQLTFATSGTLNFGQGEALMLGALVGLSVVGNI-----H 58 Query: 72 PLPLLLGGALIVSVLVTG-LYGWTVERVAYRP-LRGGPRLVPLISAIGMSIFLQNYVQIG 129 P L +I VLV G L G VE + RP ++ ++S I ++I +N + Sbjct: 59 GGPYLNYWLMIPIVLVFGALQGMFVEWIGVRPAIKIKSEFGWIMSTIALAIIFKNVAENI 118 Query: 130 QGARDMSVPVLISGALEFQMGSDFTVTV-PYSRMVIVGVTLVLMVALTLFIARSRMGRAC 188 G D+ P ISGA FQ+ F V P +V+VG L++M A+ LF +S G+A Sbjct: 119 WGKDDLPFPSPISGA-PFQI---FGANVQPMQVLVVVG-ALLIMAAVELFNRKSIYGKAV 173 Query: 189 RACAEDMGMANLLGIDTNKVISFTFVLGAMLAAVGGVLIA-LTIGKLNPYIGFIAGIKAF 247 A + D A L+GI+T+ VI+F++ L + AA GVL+A LT+ +G G+KAF Sbjct: 174 VATSNDRDAAGLMGINTSMVITFSYALSSATAAFAGVLVAPLTL--TGATMGAALGLKAF 231 Query: 248 TAAVLGGIGSIPGAMLGGVLLGLAETFAAGYLPSEYKDVVSFGLLVLILLFRPTGLLGKP 307 A++GG+ S GA++GG++LG+AET Y+ + YK+V LL+L+L +P GL GK Sbjct: 232 AVAIIGGLTSGMGAIVGGLILGVAETLTGFYISTGYKEVPGLVLLLLVLAVKPAGLFGKT 291 Query: 308 DIEKV 312 I+KV Sbjct: 292 AIKKV 296 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.145 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 888,489 Number of extensions: 35675 Number of successful extensions: 327 Number of sequences better than 1.0e-08: 31 Number of HSP's gapped: 260 Number of HSP's successfully gapped: 31 Length of query: 312 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 227 Effective length of database: 1,217,395 Effective search space: 276348665 Effective search space used: 276348665 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)