BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0012.p (408 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310993|ref|YP_003775085.1| branched-chain amino acid ABC t... 776 0.0 gi|300309520|ref|YP_003773612.1| branched-chain amino acid ABC t... 263 2e-71 gi|300310522|ref|YP_003774614.1| branched-chain amino acid ABC t... 247 2e-66 gi|300311115|ref|YP_003775207.1| branched-chain amino acid ABC t... 164 2e-41 gi|300312238|ref|YP_003776330.1| amino acid ABC transporter perm... 127 1e-30 >gi|300310993|ref|YP_003775085.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 408 Score = 776 bits (2005), Expect = 0.0, Method: Compositional matrix adjust. Identities = 390/408 (95%), Positives = 403/408 (98%) Query: 1 MPETFKNAVAAALVTAVLTIPLLGMQLQLEGYRVVLNTHWTPVLVAVLAVFLFQLARPAL 60 MPETFKNAV AALVTAVLTIPLLGMQLQLEGYRVVLNTHWTPVLVAVLAVFLFQLA+P L Sbjct: 1 MPETFKNAVTAALVTAVLTIPLLGMQLQLEGYRVVLNTHWTPVLVAVLAVFLFQLAKPVL 60 Query: 61 ARTSAGIKLPALPRMQPGQQRAAVLILLMAALVWPFFGSRGYVDVMTLALIYVVLGLGLN 120 +R+SAGIKLPALPRMQP QQRAAV+ILLMAALVWPFFGSRGYVDVMTLALIYVVLGLGLN Sbjct: 61 SRSSAGIKLPALPRMQPRQQRAAVMILLMAALVWPFFGSRGYVDVMTLALIYVVLGLGLN 120 Query: 121 IVVGFAGLLDLGYVGFYAVGAYTYALCNQYFGLSFWECVPLSAAAAALFGFLLGFPVLRL 180 IVVGFAGLLDLGYVGFYAVGAYTYALCNQYFGL+FWECVPL+AAA+ALFGFLLGFPVLRL Sbjct: 121 IVVGFAGLLDLGYVGFYAVGAYTYALCNQYFGLTFWECVPLAAAASALFGFLLGFPVLRL 180 Query: 181 RGDYLAIVTLGFGEIIRLLLNNMTSLTGGPDGISGIPKPTVFGVVMARNPLTEDGTTFHQ 240 RGDYLAIVTLGFGEIIRLLLNNMTS+TGGPDG+SGIPKPTVFG+VMARNP+TE GTTFHQ Sbjct: 181 RGDYLAIVTLGFGEIIRLLLNNMTSITGGPDGVSGIPKPTVFGLVMARNPVTEGGTTFHQ 240 Query: 241 LLGLTYQGGHMVIFLYFLALLMVLFTYFVTSRLLRMPMGRAWEALREDEIACRSLGINPT 300 L GL+YQGGHMVIFLYFLALLMVLFTYFVTSRLLRMPMGRAWEALREDEIACRSLGINPT Sbjct: 241 LFGLSYQGGHMVIFLYFLALLMVLFTYFVTSRLLRMPMGRAWEALREDEIACRSLGINPT 300 Query: 301 RVKLSAFTLGAAFAGLAGSFFAARQGLVTPESFTFIESALILAIVVLGGMGSQLGVILAA 360 +VKLSAFTLGAAFAGLAGSFFAARQGLVTPESFTFIESALILAIVVLGGMGSQLGVILAA Sbjct: 301 KVKLSAFTLGAAFAGLAGSFFAARQGLVTPESFTFIESALILAIVVLGGMGSQLGVILAA 360 Query: 361 ILLTVLPELARSFAEYRMLIFGLVMVLMMMWRPQGLLPATRPHVELPQ 408 ILLTVLPELARSFAEYRMLIFGLVMVLMMMWRPQGLLPATRPHVELPQ Sbjct: 361 ILLTVLPELARSFAEYRMLIFGLVMVLMMMWRPQGLLPATRPHVELPQ 408 >gi|300309520|ref|YP_003773612.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 404 Score = 263 bits (672), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 167/389 (42%), Positives = 227/389 (58%), Gaps = 61/389 (15%) Query: 74 RMQPGQQRAAVLILLMAALVWPFFGSR---GYVDVMTLALIYVVLGLGLNIVVGFAGLLD 130 + P Q R ++L+LL +V+PF + +V +M +AL+Y++L LGLN+VVGFAGLLD Sbjct: 9 KRNPQQARISLLLLLALMIVFPFVAQQFGNSWVRIMDVALLYIMLALGLNVVVGFAGLLD 68 Query: 131 LGYVGFYAVGAYTYAL-------------CNQY-------------------FGLSFWEC 158 LGY+ FYA+GAY+ L N Y LS W Sbjct: 69 LGYIAFYAIGAYSAGLLASPQFAAVIESFVNTYPSVGNFLVWLCGPEIVQNGIHLSLWLI 128 Query: 159 VPLSAAAAALFGFLLGFPVLRLRGDYLAIVTLGFGEIIRLLLNNMT---SLTGGPDGISG 215 VP+SA AALFG LLG P L+LRGDYLAIVTLGFGEIIR+ +NN+ ++T GP GI+ Sbjct: 129 VPISAFLAALFGALLGAPTLKLRGDYLAIVTLGFGEIIRIFMNNLNAPVNITNGPQGINL 188 Query: 216 IPKPTVFGVVMARNPLTEDGTTFHQLLGLTYQGGHMVIFLYFLALLMVLFTYFVTSRLLR 275 I VFGV +A P + ++ G++ V YFL LL+ + F + RL Sbjct: 189 IDPIKVFGVSLAGEP---GSGSMVKVFGMSMPS---VNAYYFLFLLLCIGVIFFSVRLQD 242 Query: 276 MPMGRAWEALREDEIACRSLGINPTRVKLSAFTLGAAFAGLAGSFFAARQGLVTPESFTF 335 +GRAW A+REDEIA +++GIN VKL AF +GA+F G+AG+ F A QG V+PESF+ Sbjct: 243 SRLGRAWVAIREDEIAAKAMGINTRNVKLLAFAMGASFGGVAGAMFGAFQGFVSPESFSL 302 Query: 336 IESALILAIVVLGGMGSQLGVILAAILLTVLPELARSFAE----------------YRML 379 ES +LA+VVLGG+G GV+L ++L LPE+ R E R L Sbjct: 303 TESIAVLAMVVLGGIGHIPGVVLGGVILAALPEVLRHVVEPVQMAIFGKVWIDAEVLRQL 362 Query: 380 IFGLVMVLMMMWRPQGLLPATRPHVELPQ 408 ++GL MV++M+ RP GL P+ R H + P+ Sbjct: 363 LYGLAMVVIMLTRPAGLWPSPR-HEDRPE 390 >gi|300310522|ref|YP_003774614.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 404 Score = 247 bits (630), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 167/389 (42%), Positives = 227/389 (58%), Gaps = 61/389 (15%) Query: 74 RMQPGQQRAAVLILLMAALVWPFFGSR---GYVDVMTLALIYVVLGLGLNIVVGFAGLLD 130 + P Q R ++L+LL +V+PF + +V +M +AL+Y++L LGLN+VVGFAGLLD Sbjct: 9 KRNPQQARISLLLLLALMIVFPFVAQQFGNSWVRIMDVALLYIMLALGLNVVVGFAGLLD 68 Query: 131 LGYVGFYAVGAYTYAL-------------CNQY-------------------FGLSFWEC 158 LGY+ FYA+GAY+ L N Y LS W Sbjct: 69 LGYIAFYAIGAYSAGLLASPQFAAVIESFVNTYPSVGNFLVWLCGPEIVQNGIHLSLWLI 128 Query: 159 VPLSAAAAALFGFLLGFPVLRLRGDYLAIVTLGFGEIIRLLLNNMT---SLTGGPDGISG 215 VP+SA AALFG LLG P L+LRGDYLAIVTLGFGEIIR+ +NN+ ++T GP GI+ Sbjct: 129 VPISALLAALFGALLGAPTLKLRGDYLAIVTLGFGEIIRIFMNNLNAPVNITNGPQGINL 188 Query: 216 IPKPTVFGVVMARNPLTEDGTTFHQLLGLTYQGGHMVIFLYFLALLMVLFTYFVTSRLLR 275 I VFGV +A P + ++ G++ V YFL LL+ + F + RL Sbjct: 189 IDPIKVFGVSLAGEP---GSGSMVKVFGMSMPS---VNAYYFLFLLLCIGVIFFSVRLQD 242 Query: 276 MPMGRAWEALREDEIACRSLGINPTRVKLSAFTLGAAFAGLAGSFFAARQGLVTPESFTF 335 +GRAW A+REDEIA +++GIN VKL AF +GA+F G+AG+ F A QG V+PESF+ Sbjct: 243 SRLGRAWVAIREDEIAAKAMGINTRNVKLLAFAMGASFGGVAGAMFGAFQGFVSPESFSL 302 Query: 336 IESALILAIVVLGGMGSQLGVILAAILLTVLPELARSFAE----------------YRML 379 ES +LA+VVLGG+G GV+L ++L LPE+ R E R L Sbjct: 303 TESIAVLAMVVLGGIGHIPGVVLGGVILAALPEVLRHVVEPVQMAIFGKVWIDAEVLRQL 362 Query: 380 IFGLVMVLMMMWRPQGLLPATRPHVELPQ 408 ++GL MV++M+ RP GL P+ R H + P+ Sbjct: 363 LYGLAMVVIMLTRPAGLWPSPR-HEDRPE 390 >gi|300311115|ref|YP_003775207.1| branched-chain amino acid ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 655 Score = 164 bits (414), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 98/278 (35%), Positives = 159/278 (57%), Gaps = 25/278 (8%) Query: 102 YVDVMTLALIYVVLGLGLNIVVGFAGLLDLGYVGFYAVGAYTYALCNQYFGLSFWECVPL 161 Y+ ++ LIY +L GL+IVVG+ G + LG+ G + +G+Y + G+ + VP Sbjct: 28 YLHLIETILIYAILLFGLDIVVGYTGQVSLGHAGLFGIGSYAAGVLFFKLGMPIFVTVPA 87 Query: 162 SAAAAALFGFLLGFPVLRLRGDYLAIVTLGFGEIIRLLLNNMTSLTGGPDGISGIPKPTV 221 + A A+FG +L P LR+ G YLA+VTL FG II++L+N MT LT GP GI + KP + Sbjct: 88 AIAVTAVFGAILALPALRVTGPYLAMVTLAFGTIIQILINEMTFLTEGPLGIK-LQKPMI 146 Query: 222 FGVVMARNPLTEDGTTFHQLLGLTYQGGHMVIFLYFLALLMVLFTYFVTSRLLRMPMGRA 281 G M+ V F Y +A+LMVL + V R+L+ +GRA Sbjct: 147 GGDKMSE-----------------------VQFFYLVAVLMVL-SMLVVHRILKSNLGRA 182 Query: 282 WEALREDEIACRSLGINPTRVKLSAFTLGAAFAGLAGSFFAARQGLVTPESFTFIESALI 341 ++ALR+ IA +G++ R K+ AF + AA AGL+G+ +A + ++P ++ F + + Sbjct: 183 FQALRDSPIASDCMGVSVYRYKVYAFIISAALAGLSGALYAYSEEYISPNTYNFELTIMF 242 Query: 342 LAIVVLGGMGSQLGVILAAILLTVLPELARSFAEYRML 379 L V++GG S++G ++ A+++ +LP L A +R + Sbjct: 243 LLAVIMGGRKSRIGSLIGALIVVMLPSLLSDIALFRQI 280 >gi|300312238|ref|YP_003776330.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 288 Score = 127 bits (320), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 100/300 (33%), Positives = 166/300 (55%), Gaps = 50/300 (16%) Query: 113 VVLGLGLNIVVGFA-------GLLDLGYVGFYAVGAYTYALCNQYFGLSFWECVPLSAAA 165 V+ +G+N ++ + GLL L F +GAYT +L F + F P++ AA Sbjct: 14 VIFSIGVNAMLALSIYVTLSCGLLSLANAAFMGIGAYTASLLTMQFEMPF----PIALAA 69 Query: 166 A----ALFGFLLGFPVLRLRGDYLAIVTLGFGEIIRLLLNNMTSLTGGPDGISGIPKPTV 221 AL ++G P LRL G YLA+ TLGFGE++R+++ N+ +TGGP G++G+P Sbjct: 70 GGVLPALVALVIGIPTLRLSGVYLAMATLGFGEVVRVIVLNL-DITGGPMGLNGVP---- 124 Query: 222 FGVVMARNPLTEDGTTFHQLLGLTYQGGHMVIFLYFLALLMVLFTYFVTSRLLRMPMGRA 281 P+TE ++ +L++ T +V +RL R +GRA Sbjct: 125 --------PITE----------------------WWHIVLLLGVTIYVLARLRRSKIGRA 154 Query: 282 WEALREDEIACRSLGINPTRVKLSAFTLGAAFAGLAGSFFAARQGLVTPESFTFIESALI 341 +EA++EDEIA R +G+N KL AF +GAA AG+AG A + P ++ F + I Sbjct: 155 FEAIKEDEIAARLMGVNVAGYKLLAFVIGAAIAGVAGGLNAHFTFTIGPNNYGFENAVDI 214 Query: 342 LAIVVLGGMGSQLGVILAAILLTVLPELARSFAEYRMLIFGLVMVLMMMWRPQGLLPATR 401 L + V GG + +G ++ + +L++LPE+ R F ++R+ I GL+++L++++ P+G+ R Sbjct: 215 LTMAVFGGTSNLIGPMIGSTILSLLPEVLRHFKDFRLAINGLILILVVLYLPKGIWDPRR 274 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.144 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,072,582 Number of extensions: 39413 Number of successful extensions: 274 Number of sequences better than 1.0e-08: 26 Number of HSP's gapped: 237 Number of HSP's successfully gapped: 29 Length of query: 408 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 321 Effective length of database: 1,207,925 Effective search space: 387743925 Effective search space used: 387743925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)