BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0007.p (534 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311000|ref|YP_003775092.1| glucose dehydrogenase [Herbaspi... 1098 0.0 gi|300313052|ref|YP_003777144.1| choline dehydrogenase [Herbaspi... 410 e-115 gi|300313761|ref|YP_003777853.1| gluconate dehydrogenase [Herbas... 78 2e-15 >gi|300311000|ref|YP_003775092.1| glucose dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 534 Score = 1098 bits (2841), Expect = 0.0, Method: Compositional matrix adjust. Identities = 522/534 (97%), Positives = 531/534 (99%) Query: 1 MSNPNSADVVVIGAGVTGALLATRLAKTGKSVLLLESGPRMGRDQLVERFRSSAFKSDFM 60 MSN NSADVVVIGAGVTGALLATRLAKTGKSVLLLESGPRMGRDQLVERFRSSAFKSDFM Sbjct: 1 MSNLNSADVVVIGAGVTGALLATRLAKTGKSVLLLESGPRMGRDQLVERFRSSAFKSDFM 60 Query: 61 APYPYSPLAPHPRYTPEPNGYLQQKGPHAFNAQYIRMFGGTSWHWAAQLWRYVPNDFRQH 120 APYPYSPLAPHPRYTPEPNGYLQQKGPHAFNAQYIRMFGGTSWHWAAQLWRYVPNDFRQH Sbjct: 61 APYPYSPLAPHPRYTPEPNGYLQQKGPHAFNAQYIRMFGGTSWHWAAQLWRYVPNDFRQH 120 Query: 121 SLYGVGKDWPISYDDLEDYYYQAEVIAGVGGSPESGSPRKHPFPMERVPASWLQQRVTAR 180 SLYGVGKDWPISYDDLEDYYYQAEVIAGVGGSPESGSPRKHPFPMERVPASWLQQRVTAR Sbjct: 121 SLYGVGKDWPISYDDLEDYYYQAEVIAGVGGSPESGSPRKHPFPMERVPASWLQQRVTAR 180 Query: 181 LAPEFTVLDDSTGRNSRNYDGRPACCGNNNCMPLCPIDAQYHGGLAAQQAEDSGVQVITE 240 LAPEFTVLDDSTGRNSRNYDGRPACCGNNNCMPLCPIDAQYHGGLAAQQAEDSGV+VITE Sbjct: 181 LAPEFTVLDDSTGRNSRNYDGRPACCGNNNCMPLCPIDAQYHGGLAAQQAEDSGVKVITE 240 Query: 241 AVVYRLEHDEKGRIAAVHYYDWNKVSHRITAQTFVLAANAIETPKLLLMSVSDKFPQGIA 300 AVVYRLEHDEKGRIAA HYYDWNKVSHRITAQTFVLAANAIETPKLLLMS+SD+FPQGIA Sbjct: 241 AVVYRLEHDEKGRIAAAHYYDWNKVSHRITAQTFVLAANAIETPKLLLMSISDRFPQGIA 300 Query: 301 NARDNVGRNLCDHPAIGVTFDVDEEIWPGRGPVSPCSIGQFRDGDFRAEHAPFRIDISNA 360 NARDNVGRNLCDHPAIGV+FDVDEEIWPGRGPVSPCSIGQFRDGDFRAEHAPFRIDISNA Sbjct: 301 NARDNVGRNLCDHPAIGVSFDVDEEIWPGRGPVSPCSIGQFRDGDFRAEHAPFRIDISNA 360 Query: 361 SQVASVTKEVLAEGYFGKRLEEQILFRAARRLSIKNALEQLPDRNNRVTLGDKKDELGLP 420 SQVA+VTKEV+AEGYFGKRLEEQILFRAARRLSIKNALEQLPDRNNRVTLGDKKDELGLP Sbjct: 361 SQVAAVTKEVVAEGYFGKRLEEQILFRAARRLSIKNALEQLPDRNNRVTLGDKKDELGLP 420 Query: 421 VPALYWNVDDYVVKGTEKTRAAYDAIAARLGATNIKHSKSGAFSNRQHITGTLSMGLDPA 480 VPALYWNVDDYV+KGTE+TRA YDAIAA+LGATNIKHSKSGAFSNRQHITGTLSMGLDPA Sbjct: 421 VPALYWNVDDYVIKGTEQTRACYDAIAAKLGATNIKHSKSGAFSNRQHITGTLSMGLDPA 480 Query: 481 TSVTDAWGRAHDHENLFMVGTGVMPTVGTCNVTLTAMALALRTADKIIEESRHA 534 TSVTDAWGRAHDHENLFMVGTGVMPTVGTCNVTLTAMALALRTADKIIEESRHA Sbjct: 481 TSVTDAWGRAHDHENLFMVGTGVMPTVGTCNVTLTAMALALRTADKIIEESRHA 534 >gi|300313052|ref|YP_003777144.1| choline dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 543 Score = 410 bits (1053), Expect = e-115, Method: Compositional matrix adjust. Identities = 236/545 (43%), Positives = 319/545 (58%), Gaps = 13/545 (2%) Query: 1 MSNPNSADVVVIGAGVTGALLATRLAKTGKSVLLLESGPRMGRDQLVERFRSS--AFKSD 58 M++ N A+VV++G GV G L+A +L G V +LE+GPR+ R ++VE FR+ A K D Sbjct: 1 MNSNNKAEVVIVGTGVVGVLIAEQLLNAGIPVRMLEAGPRVARAEVVENFRNLPLALKGD 60 Query: 59 FMAPYPYSPLAPHPRYTPE--PNGYLQQKGPHAFNAQYIRMFGGTSWHWAAQLWRYVPND 116 + YP P APHP + E + YL+ GP + +IR GG++WHWA WR P D Sbjct: 61 PSSCYPPRPWAPHPMPSSENPDDHYLELSGPDKYAQTFIRYAGGSTWHWAGTCWRLTPED 120 Query: 117 FRQHSLYGVGKDWPISYDDLEDYYYQAEVIAGVGGSPESG----SPRKHPFPMERVPASW 172 R HSLYGVG+DW YD LE YY + E G+ G + R P+PM +P S Sbjct: 121 MRLHSLYGVGRDWAFGYDVLEPYYTRVEHQLGICGPSDPALQWPPQRSAPYPMPALPFSP 180 Query: 173 LQQRVTARL-APEFTVLDDSTGRNS-RNYDGRPACCGNNNCMPLCPIDAQYHGGLAAQQA 230 +QR T + A + + + RNS YD RPACC NNNC+P+CP+ A+Y A + Sbjct: 181 GEQRFTEVVQALGYRNIPTAQARNSGMPYDDRPACCANNNCVPVCPVGAKYDAATALARL 240 Query: 231 EDSGVQVITEAVVYRLEHDEKGRIAAVHYYDWNKVSHRITAQTFVLAANAIETPKLLLMS 290 E G ++T+AVVYR++ D R+ AV+Y+D NK+SHRIT + FVLA N IETPKLLLMS Sbjct: 241 EAKGATILTDAVVYRVDTDANDRVEAVNYFDANKLSHRITGKIFVLACNGIETPKLLLMS 300 Query: 291 VSDKFPQGIANARDNVGRNLCDHPAIGVTFDVDEEIWPGRGPVSPCSIGQFRDGDFRAEH 350 + + P G+AN D VGRN+ DHP + +T + E W G GPV I + G FR+EH Sbjct: 301 ANSRNPHGLANRSDQVGRNMMDHPQLTMTLTLSEPYWSGVGPVVNSGIMETSQGAFRSEH 360 Query: 351 APFRIDISNASQVASVTKEVLAEGYFGKRLEEQILFRAARRLSIKNALEQLPDRNNRVTL 410 A +N ++ VT L +G G+ L+E+I A I A E LPD NR+TL Sbjct: 361 AGAYFRYNNFARNRFVTFAALKKGLVGQALDEEIRRMTACTADIVLAHEVLPDAENRLTL 420 Query: 411 GDKKDELGLPVPALYWNVDDYVVKGTEKTRAAYD-AIAARLGATNIKHSKSGAFSNRQHI 469 KKD LGLP PA++++V DY + E IA +GAT+IK S F+ +HI Sbjct: 421 SSKKDWLGLPRPAIHYDVGDYTRRSAELYSLPIGRKIAEAMGATDIKVSPK--FAQSKHI 478 Query: 470 TGTLSMGLDPATSVTDAWGRAHDHENLFMVGTGVMPTVGTCNVTLTAMALALRTADKIIE 529 G MGLDPA SV D RAHDH+NL++ G G MP+ N T++ AL L+ AD I+ Sbjct: 479 MGGTIMGLDPANSVVDPDCRAHDHDNLYLPGGGAMPSTACGNSTISMAALGLKAADAILR 538 Query: 530 ESRHA 534 + + A Sbjct: 539 QLQKA 543 >gi|300313761|ref|YP_003777853.1| gluconate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 593 Score = 78.2 bits (191), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 143/605 (23%), Positives = 236/605 (39%), Gaps = 98/605 (16%) Query: 1 MSNPNS--ADVVVIGAGVTGALLATRLAKTGKSVLLLESGPRMGRDQLVERFRSSAFKSD 58 M+N N DVV++G G TGA++A + + G+ V+ LE G + RD D Sbjct: 1 MANKNMKPVDVVIVGFGWTGAIMAKEMTEAGQRVVALERG--VYRDTY----------PD 48 Query: 59 FMAPYPYSPLAPHPRYTPEPN------GYLQQKGPHAFNAQYIRMF------GGTSWHWA 106 P + L R+ N + ++ G A + I MF GG HW+ Sbjct: 49 GAYPKTINELEYQQRFKLFQNLNKSSFTFRRKTGDSAIPYRQIAMFKPGEGVGGAGLHWS 108 Query: 107 AQLWRYVPNDFRQHSLYG--VGK----------DWPISYDDLEDYYYQAEVIAGVGGSP- 153 WR +P + R S Y GK DW +SY++LE Y+ AE + G G+ Sbjct: 109 GCHWRILPEELRMRSHYEERYGKNFIPKDMTLQDWGVSYEELEPYFDFAEKMMGTSGTAY 168 Query: 154 --------ESGSP----RKHPFPM----ERVPASWLQQRVTARLAPEFTVLDDSTGRNSR 197 ESG+P R FP+ E+ A+ ++ FT+ + Sbjct: 169 RVGGKVVDESGNPFEADRSDNFPLPAQKEQYQAALFRKAAQQAGLHPFTLPSANAAAPYV 228 Query: 198 NYDGRPACCGNNNCM--PLCPIDAQYHGGLAAQQA-------EDSGVQVITEAVVYRLEH 248 N G C C C A Y+ A+ + + ++ + V R+E Sbjct: 229 NQYG----CQMGPCTFCGFCSGYACYNYSKASPNVNIMPALRQSNLFELRSSCNVLRIEL 284 Query: 249 D-EKGRIAAVHYYDWNKVSHRITAQTFVLAANAIETPKLLLMSVSDKFPQGIANARDNVG 307 D + + V Y D N + A + + A +L L+S K ++N VG Sbjct: 285 DSSRKKATGVTYVDINGDTVFQPASIVIASTFAYNNARLFLLSGIGKPYDPVSNT-GAVG 343 Query: 308 RNLCDHPAIGVTFDVDEEIWPGR---GPVSPCSIGQFRDGDFRAEH-----------APF 353 RN+ A + ++ PG+ G + G D DF +H +P Sbjct: 344 RNI----AFQMMSTINAFFEPGKNINGFIGAGGNGVAVD-DFNGDHMDHGPLGFVGGSPI 398 Query: 354 RIDISNASQVASVTKEVLAEGYFGKRLEEQILFRAARRLSIKNALEQLPDRNNRVTLG-D 412 + S + ++ + +G+ ++ + +S + R+ V L + Sbjct: 399 WCNPSGSKPISGIAVPS-GTPRWGRAWKQAVKDSYLNTMSFDVHGANMVYRDVYVDLDPN 457 Query: 413 KKDELGLPVPALYWNVDDYVVKGTEKTRAAYDAIAARLGATNIKHSKS--GAFSNRQ--- 467 KD G P+ ++ D ++ + IA L A IK + GA + + Sbjct: 458 YKDAFGQPLLRFTFDWKDNDIRMSRYVTDQAVKIAHELKAREIKVTVKDFGAAPDLRSYQ 517 Query: 468 --HITGTLSMGLDPATSVTDAWGRAHDHENLFMVGTGVMPTVGTCNVTLTAMALALRTAD 525 H G ++MG DP+TSV + + ++ D N+F VG+GV N T A+AL +A Sbjct: 518 TTHWAGGVAMGTDPSTSVVNRYLQSWDVHNVFAVGSGVFAQGIGYNPTGAAVALTYWSAR 577 Query: 526 KIIEE 530 I E+ Sbjct: 578 AIREQ 582 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,786,062 Number of extensions: 81160 Number of successful extensions: 270 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 264 Number of HSP's successfully gapped: 3 Length of query: 534 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 444 Effective length of database: 1,193,720 Effective search space: 530011680 Effective search space used: 530011680 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)