BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0003.p (397 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314020|ref|YP_003778112.1| major facilitator superfamily p... 262 4e-71 gi|300309872|ref|YP_003773964.1| major facilitator superfamily p... 181 9e-47 gi|300310323|ref|YP_003774415.1| methylenomycin A resistance pro... 122 7e-29 gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 100 2e-22 gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 92 5e-20 >gi|300314020|ref|YP_003778112.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 475 Score = 262 bits (669), Expect = 4e-71, Method: Compositional matrix adjust. Identities = 151/309 (48%), Positives = 196/309 (63%), Gaps = 8/309 (2%) Query: 5 SASSSTSPDHH-----DGLPPRQRLLAIATVVLGLSIAVLDGSIANLALPVMATDLKASD 59 +A + +P H +GLPP QR AI + + + +A LD +IAN ALP MA DL+ + Sbjct: 6 TAPGTAAPSPHIVIAGEGLPPEQRGRAILALGIAVGLATLDTAIANTALPAMALDLQTTP 65 Query: 60 ADAIWVINSYQIAMAVCLLPLASLGEVIGYRRVYLGGLFLFTLASLACAASGDMLQLSLA 119 A ++W++ +YQ+AM V LLPLA+LGE+IGYRR+Y+ GL LFT ASL CA + + L +A Sbjct: 66 AASVWIVTAYQLAMMVALLPLAALGEIIGYRRIYIWGLVLFTAASLLCAVAWSLPTLVMA 125 Query: 120 RVLQGLGAAGILSVNTALIRFIYPARQLGRAIGYNALVAGTGNALGPTVAGVILHLGSWH 179 R LQGLG AGI+SVNTALIRFIYP R LGR +G N L+ +GP+VA IL + W Sbjct: 126 RFLQGLGGAGIMSVNTALIRFIYPTRSLGRGVGLNTLIVAIAFTVGPSVASGILAIAPWP 185 Query: 180 WLFLINIPLGLAALAVGSRALPFNPLSARRFDRQSALLCMAFIGLLLYTIDAIGHLQGGW 239 WLF +N+PLG+ A+ + +LP L+ FD +SALL A GLL+ I H Q Sbjct: 186 WLFAVNVPLGVLAVVLAVPSLPHTSLARHAFDLRSALLNAAAFGLLILAIGEGAH-QAQA 244 Query: 240 VVAGCALLTAI--SLALLLKLQSPSAPMLPLDLLRIPLFALSIGTSFCSFAAQMAVFVML 297 V G L AI L LL + S APMLP+DL R P+F+LS T+ CSFAAQ FV L Sbjct: 245 SVVGLELAAAILCGLFLLKRELSHPAPMLPVDLFRHPMFSLSAVTAVCSFAAQGLAFVSL 304 Query: 298 PFMLQNHLG 306 PF + LG Sbjct: 305 PFFFHHDLG 313 >gi|300309872|ref|YP_003773964.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 493 Score = 181 bits (459), Expect = 9e-47, Method: Compositional matrix adjust. Identities = 120/312 (38%), Positives = 170/312 (54%), Gaps = 11/312 (3%) Query: 13 DH--HDGLPPRQRLLAIATVVLGLSIAVLDGSIANLALPVMATDLKASDADAIWVINSYQ 70 DH +GLP +QR A+ V+ ++AV DG+I N+ALP ++ L+ S A +IWV N+Y Sbjct: 15 DHPNDEGLPGKQRRRAMLAVMTATTMAVFDGTIINVALPQISQALQTSAAVSIWVSNAYL 74 Query: 71 IAMAVCLLPLASLGEVIGYRRVYLGGLFLFTLASLACAASGDMLQLSLARVLQGLGAAGI 130 +A A+ L A+L IG+R + GL +FTLASL CA SG + L L R+LQGLG A + Sbjct: 75 LATAMTLAAFAALSSRIGFRAQFSAGLAVFTLASLGCALSGSVTLLILMRILQGLGGAAM 134 Query: 131 LSVNTALIRFIYPARQLGRAIGYNALVAGTGNALGPTVAGVILHLGSWHWLFLINIPLGL 190 LS+ A+ R I+P R LGR +G NA++ A+GP + G +L + W W+F IN+PLG+ Sbjct: 135 LSIAPAIHRTIFPNRLLGRILGLNAVLVAASTAVGPVLGGTLLAMLGWEWIFAINVPLGV 194 Query: 191 AALAVGSRALPFNPLSAR-RFDRQSALLCMAFIGLLLYTIDAIGHL----QGG----WVV 241 A+ + A+P A+ FD AL +G L+ D QG Sbjct: 195 LAVWLAWNAVPDTRKPAQAAFDLAGALWSALAMGALIMAADTCARFAEPGQGSGAAFTAA 254 Query: 242 AGCALLTAISLALLLKLQSPSAPMLPLDLLRIPLFALSIGTSFCSFAAQMAVFVMLPFML 301 A LA + + + P+LPLD+ P F+L+ TS SF Q FV LPF+ Sbjct: 255 AYAVTALLAGLAFVRGQRRAADPLLPLDIFANPRFSLAALTSLASFVGQGIAFVALPFLF 314 Query: 302 QNHLGMGAARAA 313 QN G A +A Sbjct: 315 QNAYGYTAFESA 326 >gi|300310323|ref|YP_003774415.1| methylenomycin A resistance protein [Herbaspirillum seropedicae SmR1] Length = 478 Score = 122 bits (305), Expect = 7e-29, Method: Compositional matrix adjust. Identities = 96/294 (32%), Positives = 152/294 (51%), Gaps = 14/294 (4%) Query: 18 LPPRQRLLAIATVVLGLSIAVLDGSIANLALPVMATDLKASDADAIWVINSYQIAMAVCL 77 L PR +L + + +G +A+LD ++ N+ L +A L A + +W+++ Y + A L Sbjct: 23 LTPRAGMLPLVALAIGFVMAMLDVTVVNVGLSNIAGSLDAPLSTLVWIVDGYTLTFAAML 82 Query: 78 LPLASLGEVIGYRRVYLGGLFLFTLASLACAASGDMLQLSLARVLQGLGAAGILSVNTAL 137 L +L + G RR+YL GLFLF LASL C A+ + L +AR+LQGLGAA + + +L Sbjct: 83 LVGGALADRFGARRLYLTGLFLFVLASLLCGAAPNGPFLIVARLLQGLGAAFFMPSSLSL 142 Query: 138 IRFIYP-ARQLGRAIGYNALVAGTGNALGPTVAGVILHLGSWHWLFLINIPLGLAALAVG 196 + +Y R R +G + G A+GP V GV++H W +FLIN+P+GL L + Sbjct: 143 LTHVYEDDRVRARMLGVWSATVGLAAAVGPLVGGVLIHWLGWRSVFLINVPVGLVGLVMA 202 Query: 197 SRALPFNPLSARRFDRQSALLCMAFIGLLLYTIDAIGHLQG---GW----VVAGCALLTA 249 + +P AR + S L + + L + + +QG GW ++AG A+ + Sbjct: 203 RKLIPQVGGHARALNLSSHLAGVVMLAGLSFVL-----IQGPVYGWTSPRILAGLAVALS 257 Query: 250 ISLALLLKLQSPSAPMLPLDLLRIPLFALSIGTSF-CSFAAQMAVFVMLPFMLQ 302 AL+ AP+LP L P FA + G F +F +F + F+ Q Sbjct: 258 AGAALIRHELRGIAPLLPKALFATPQFAAANGIGFLINFGVYGNLFYLALFLQQ 311 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 100 bits (250), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 82/277 (29%), Positives = 131/277 (47%), Gaps = 3/277 (1%) Query: 39 LDGSIANLALPVMATDLKASDADAIWVINSYQIAMAVCLLPLASLGEVIGYRRVYLGGLF 98 LD +I ALP MA + + +Y + +AV + + + G R V+ + Sbjct: 45 LDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIPISGWVADRFGSRTVFGMAIA 104 Query: 99 LFTLASLACAASGDMLQLSLARVLQGLGAAGILSVNTALIRFIYPARQLGRAIGYNALVA 158 +FTLAS+ C S ++ + + ARVLQGLG A ++ V ++ +L +AI A Sbjct: 105 IFTLASILCGFSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRSTGKDRLMKAIATITWPA 164 Query: 159 GTGNALGPTVAGVILHLGSWHWLFLINIPLGLAAL-AVGSRALPFNPLSARRFDRQSALL 217 +GP++ G I SWHW+FL+N+P GLAA AV + +S R D LL Sbjct: 165 IVAPVVGPSIGGFITTYASWHWIFLMNVPFGLAAFTAVMMVVRNEHGVSKRPLDLVGFLL 224 Query: 218 CMAFIGLLLYTIDAIGHLQGGWVVA--GCALLTAISLALLLKLQSPSAPMLPLDLLRIPL 275 A + LLY + H++ VA AL + +Q P+L LRIP Sbjct: 225 SGAALTSLLYGTEIASHMESNIKVALGFVALGVVLGYGAWRHMQRIEHPLLDFSNLRIPT 284 Query: 276 FALSIGTSFCSFAAQMAVFVMLPFMLQNHLGMGAARA 312 +++++ T + AV ++P M Q G+ A ++ Sbjct: 285 YSVTVLTGSLTRIGVDAVPYLMPLMFQIGFGLTAFQS 321 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 92.4 bits (228), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 78/281 (27%), Positives = 132/281 (46%), Gaps = 9/281 (3%) Query: 36 IAVLDGSIANLALPVMATDLKASDADAIWVINSYQIAMAVCLLPLASLGEVIGYRRVYLG 95 IAVLD +IAN+++ +A L AS + +VI SY +A A+ + L G RV++ Sbjct: 44 IAVLDTTIANVSVSTIAGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFIL 103 Query: 96 GLFLFTLASLACAASGDMLQLSLARVLQGLGAAGILSVNTALIRFIYPARQLGRAIGYNA 155 + +F L S C + + L RVLQGL ++ ++ L+ I+P + A+G A Sbjct: 104 SMVMFGLFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAPAAVGLWA 163 Query: 156 LVAGTGNALGPTVAGVILHLGSWHWLFLINIPLGLAALAVGSRALP--FNPLSARRFDRQ 213 + GP + GV+ SW ++F IN+P+ L +G R L +S DR Sbjct: 164 MTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYIGWRMLKRYETKMSKLPIDRV 223 Query: 214 SALLCMAFIGLLLYTIDAIGHLQGGWVVAGCALLTAIS-----LALLLKLQSPSAPMLPL 268 +L + ++ L +D + W + +L A++ +A L+ + P++ L Sbjct: 224 GLVLMVVWVAALQLMLDE--GKEKDWFASTEIVLLAVTAVVFFIAFLIWELTERNPIVDL 281 Query: 269 DLLRIPLFALSIGTSFCSFAAQMAVFVMLPFMLQNHLGMGA 309 + R F S+ T +F A V+ P LQ ++G A Sbjct: 282 RVFRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTA 322 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.140 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,159,798 Number of extensions: 47508 Number of successful extensions: 325 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 318 Number of HSP's successfully gapped: 15 Length of query: 397 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 310 Effective length of database: 1,207,925 Effective search space: 374456750 Effective search space used: 374456750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)