BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0093.p (305 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312102|ref|YP_003776194.1| LysR family transcription regul... 487 e-139 gi|300312305|ref|YP_003776397.1| LysR family transcription regul... 100 2e-22 gi|300312111|ref|YP_003776203.1| LysR family transcription regul... 86 3e-18 gi|300312835|ref|YP_003776927.1| LysR family transcription regul... 84 1e-17 gi|300310436|ref|YP_003774528.1| LysR family transcription regul... 80 2e-16 >gi|300312102|ref|YP_003776194.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 305 Score = 487 bits (1254), Expect = e-139, Method: Compositional matrix adjust. Identities = 263/303 (86%), Positives = 278/303 (91%) Query: 1 MRELSLDRLRTLVAIADLGSFAAAARELHLAPPTVSLHISELEERIGAPLLLRQRSQVRP 60 MRELSLDRLRTLVAIADLGSFAAAARELHLAPPTVSLHISELEERIGAPLLLRQR+QVRP Sbjct: 1 MRELSLDRLRTLVAIADLGSFAAAARELHLAPPTVSLHISELEERIGAPLLLRQRAQVRP 60 Query: 61 SGIGATLIARARQLLAAADQALEEVGRQVLGLAGRVRLGASTGAIATLLPQVLERLGQRH 120 SGIG TLIARARQLLAAADQALEEVGRQV GLAGRVRLGASTGAIA LLPQ LE+LG+ H Sbjct: 61 SGIGETLIARARQLLAAADQALEEVGRQVQGLAGRVRLGASTGAIAYLLPQALEQLGRSH 120 Query: 121 PDIDVQIHILTSQETLSQLNEGLLDIGLVALPQSPVSGLTVRPWRRDPVMAFLPAAWHAP 180 P IDVQ+ +LTSQETL++L G LDIGLVALPQ+PV+GLTV+PWRRDPVMAFLPA+W AP Sbjct: 121 PGIDVQVMVLTSQETLARLAAGALDIGLVALPQAPVAGLTVQPWRRDPVMAFLPASWRAP 180 Query: 181 RLVTPGWLAAQPLILNAPETRLSRLTSEWFAGAGEQPEARIQLNFNDAIKSLVAAGYGAT 240 R+VTP WLAAQPLILNAPETRLSRLT+EWFA GE P ARIQLNFNDAIKSLV AGYGAT Sbjct: 181 RVVTPAWLAAQPLILNAPETRLSRLTTEWFARGGEHPVARIQLNFNDAIKSLVGAGYGAT 240 Query: 241 LLPHEASAQGRPVPLDPRIVMRPLRPALWRPLGIAHRASQLEPATRHVLEALWELKQLKT 300 LLPHEASAQG V LDPRIVMRPLRPALWRPLGIAHR Q EPATRHVLEALW LKQL+ Sbjct: 241 LLPHEASAQGAAVALDPRIVMRPLRPALWRPLGIAHRTGQPEPATRHVLEALWALKQLRQ 300 Query: 301 PRS 303 PR+ Sbjct: 301 PRT 303 >gi|300312305|ref|YP_003776397.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 297 Score = 99.8 bits (247), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 75/260 (28%), Positives = 126/260 (48%), Gaps = 6/260 (2%) Query: 4 LSLDRLRTLVAIADLGSFAAAARELHLAPPTVSLHISELEERIGAPLLLRQRSQVRPSGI 63 L LR + I + GS A+ H+A + S I +E+ +G PLL R R V+ + Sbjct: 5 FELSDLRLFLHIVESGSITGGAQRAHMALASASARIRNMEDALGTPLLERGRRGVQTTDA 64 Query: 64 GATLIARARQLLAAADQALEEVGRQVLGLAGRVRLGASTGAIATLLPQVLERLGQRHPDI 123 G L AR + +Q +E+G+ GL G +RL +T A+ LP+VL R RHP Sbjct: 65 GRALAHHARMMAQQMEQLRDELGQYARGLKGHIRLLCNTSAMTEFLPEVLARFLARHPHT 124 Query: 124 DVQIHILTSQETLSQLNEGLLDIGLVALPQSPVSG-LTVRPWRRDPVMAFLPAAWHAPRL 182 D+++ S E + + +G+ D+G++A S SG L P+R+D ++A L R Sbjct: 125 DIELEEKLSYEVVQAVIDGVADVGIIA--DSTDSGPLQTYPFRQDHLVAVLSKRHPLARR 182 Query: 183 VTPGW--LAAQPLILNAPETRLSRLTSEWFAGAGEQPEARIQLNFNDAIKSLVAAGYGAT 240 + + L + + + ++ L + S AG++ + R++L D I +V G Sbjct: 183 KSLAFAELLGEDFVGLSGDSALQQHLSGHATRAGKRLQYRVRLRSFDGICRMVEHQVGVA 242 Query: 241 LLPHEASAQGRPVPLDPRIV 260 +LP A+ P +D R + Sbjct: 243 VLPETAALASTP-HMDIRAI 261 >gi|300312111|ref|YP_003776203.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 321 Score = 86.3 bits (212), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 68/248 (27%), Positives = 117/248 (47%), Gaps = 7/248 (2%) Query: 4 LSLDRLRTLVAIADLGSFAAAARELHLAPPTVSLHISELEERIGAPLLLRQRSQVRPSGI 63 L LR + IA+ S +A ++L+ S I ELE R G LL R+ V+ + Sbjct: 3 FDLTDLRLFILIAEHNSLTRSAERMNLSLGATSNRIKELETRFGTRLLYRESKGVQLTPA 62 Query: 64 GATLIARARQLLAAADQALEEVGRQVLGLAGRVRLGASTGAIATLLPQVLERLGQRHPDI 123 G TL+ A+Q + + ++ + G+ G +R+ A+T A+ +P++L + RHP + Sbjct: 63 GHTLLHHAQQFMQQVEHLKSDMQQYNNGIKGYIRIFANTTAVTEFMPEILGKFLARHPQV 122 Query: 124 DVQIHILTSQETLSQLNEGLLDIGLVALPQSPVSGLTVRPWRRDPVMAFLPAAWH----A 179 +V + + + L + EG DIG+VA P GL + + D + A H A Sbjct: 123 NVALEERLNHDILRGIQEGTADIGIVAGPVQG-EGLQILNFSTDR-LVLATALEHPLADA 180 Query: 180 PRLVTPGWLAAQPLILNAPETRLSRLTSEWFAGAGEQPEARIQLNFNDAIKSLVAAGYGA 239 P L L + + L+ T L S+ A G++ + RIQ+ +A+ ++ G Sbjct: 181 PSLPFIQTLEYEHIGLHEGST-LHHFLSKIVAENGQRLKLRIQVRSFEAMCRMIETNVGI 239 Query: 240 TLLPHEAS 247 +LP A+ Sbjct: 240 GILPRSAA 247 >gi|300312835|ref|YP_003776927.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 283 Score = 84.0 bits (206), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 56/177 (31%), Positives = 89/177 (50%), Gaps = 12/177 (6%) Query: 1 MRELSLDRLRTLVAIADLGSFAAAARELHLAPPTVSLHISELEERIGAPLLLRQRSQVRP 60 M+ L LD L +VA+AD G+F+ AA +H VS+ I LE +G PL LR+ V P Sbjct: 1 MKTLDLDVLAMIVAVADTGNFSRAAELVHRTQSAVSMQIKTLELALGKPLFLRKPRSVVP 60 Query: 61 SGIGATLIARARQLLAAADQALEEVGRQVLGLAGRVRLGASTGAIATLLPQVLERLGQRH 120 + G L+ AR++L D+A V R + GRV +G ++LLP +L++ + Sbjct: 61 TQDGEVLLTYARRMLTLRDEAWAAVVRP--DVTGRVVIGVPDDYASSLLPSILKKFSATY 118 Query: 121 PDIDVQIHILTSQETLSQLNEGLLDIGLVALPQSPVSGLTVRPWRRDPVMAFLPAAW 177 P +++Q+ L S + +G +D+ + A +SG +R F P W Sbjct: 119 PKVEIQVVGLPSSALAPMVKDGTVDL-VCATRVKGISGEFIR---------FEPMVW 165 >gi|300310436|ref|YP_003774528.1| LysR family transcription regulator [Herbaspirillum seropedicae SmR1] Length = 300 Score = 80.5 bits (197), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 81/256 (31%), Positives = 122/256 (47%), Gaps = 9/256 (3%) Query: 4 LSLDRLRTLVAIADLGSFAAAARELHLAPPTVSLHISELEERIGAPLLLRQRSQVRPSGI 63 L L +LR A+A+ F AA LH+ P +S I+ LEE +G PL R + V + Sbjct: 7 LELRQLRYFAAVAEEMHFGRAAARLHMTQPPLSQTIAALEELLGTPLFQRTKRSVALTAA 66 Query: 64 GATLIARARQLLAAADQALEEVGRQVLGLAGRVRLGASTGAIATLLPQVLERLGQRHPDI 123 GA L+ A++LL A + V R G +G + L + A ++LP +L+R +P + Sbjct: 67 GAALLPEAQRLLQQAAALPDLVRRAASGASGLLSLSFISSADYSILPPLLQRFRAHYPQV 126 Query: 124 DVQIHILTSQETLSQLNEGLLDIGLV--ALPQSPVSGLTVRPWRRDPVMAFLPAAWHAPR 181 +++ T+ L L +G +D GL+ LP+ + L VRP +P++A LP R Sbjct: 127 QIELREATTDVQLEDLLQGKIDAGLLLPPLPEKAAALLEVRPVLSEPLIAALPEGAVRKR 186 Query: 182 -LVTPGWLAAQPLILN----APETRLSRLTSEWFAGAGEQPEARIQLNFNDAIKSLVAAG 236 V+ L PLI+ AP + L+ F AG P + I LV+AG Sbjct: 187 GPVSLKSLRELPLIIFPRRIAPAFHDTILS--CFRDAGVTPHIGQEAIQMQTIVGLVSAG 244 Query: 237 YGATLLPHEASAQGRP 252 G L+P S RP Sbjct: 245 MGMALVPQSVSNLKRP 260 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,027,489 Number of extensions: 45270 Number of successful extensions: 307 Number of sequences better than 1.0e-08: 68 Number of HSP's gapped: 278 Number of HSP's successfully gapped: 68 Length of query: 305 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 220 Effective length of database: 1,217,395 Effective search space: 267826900 Effective search space used: 267826900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)