BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0084.p (349 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312093|ref|YP_003776185.1| hypothetical protein Hsero_2782... 555 e-159 gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 142 6e-35 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 139 3e-34 gi|300312026|ref|YP_003776118.1| signal transduction protein con... 139 4e-34 gi|300310189|ref|YP_003774281.1| transmembrane protein [Herbaspi... 138 6e-34 >gi|300312093|ref|YP_003776185.1| hypothetical protein Hsero_2782 [Herbaspirillum seropedicae SmR1] Length = 601 Score = 555 bits (1430), Expect = e-159, Method: Compositional matrix adjust. Identities = 298/350 (85%), Positives = 319/350 (91%), Gaps = 2/350 (0%) Query: 1 LIIASSDEALLYSQLPPVGKDPLMDRALQQGFRGNGVGLAANGINEIKAFASIPGTSWLV 60 +I+ASSDEAL+Y++LP G+D LMD+ALQ GFRGNGVGLAANG+NEIKAFA +PGTSWLV Sbjct: 253 MIVASSDEALVYTRLPATGQDLLMDQALQ-GFRGNGVGLAANGVNEIKAFAPVPGTSWLV 311 Query: 61 AVTLPLADALPTVERTRALIGRGGLIQGAMVILLVTLAYLWFFRPLRRAAALADRMTRGE 120 A TLPLA ALPTVERTRALI RGGLIQ AMV+LLV LAYLWFFRPLRRAAALADRMTRGE Sbjct: 312 AATLPLAAALPTVERTRALIARGGLIQAAMVVLLVVLAYLWFFRPLRRAAALADRMTRGE 371 Query: 121 LPLSPLPVERRDEVGHLTMAFNGLLSRLKLHQAELQHQAHHDVLTGLPNRMMLAERMEAA 180 LPLSPLPV+RRDEVG LTMAFNGLL+RLKLHQAELQHQAHHDVLTGLPNRMMLAERM+AA Sbjct: 372 LPLSPLPVDRRDEVGQLTMAFNGLLARLKLHQAELQHQAHHDVLTGLPNRMMLAERMQAA 431 Query: 181 LTHACRERNGVALLFLDLDGFKPINDTLGHKAGDQVLQEVTRRLRQVARHGDTLARVGGD 240 LTHACRERNGV LLFLDLDGFKPINDTLGHKAGDQVLQE+TRRLRQVARHGDTLARVGGD Sbjct: 432 LTHACRERNGVGLLFLDLDGFKPINDTLGHKAGDQVLQEITRRLRQVARHGDTLARVGGD 491 Query: 241 EFVLLITDLGQPYENGAQVLAEKCIRVVSEPLHLAQGEYQLGVSVGIAVCDGSCDADSLL 300 EFVLL+TDL P+E GAQ LAEKCI+ VSEP+ L Q +Y+LGVSVGIAVCDGSCDADSLL Sbjct: 492 EFVLLVTDLRLPFEQGAQALAEKCIKTVSEPICLDQRQYRLGVSVGIAVCDGSCDADSLL 551 Query: 301 QAADKAMYAAKGNGRGCYVIAPAWPGVPGE-LSAPAGTAGMTRPAGSAHS 349 QAAD+AMYAAK NGRGCYVIAPA P LSAPA TAG TRPA SAHS Sbjct: 552 QAADQAMYAAKANGRGCYVIAPARPACANTALSAPAETAGTTRPADSAHS 601 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 142 bits (357), Expect = 6e-35, Method: Compositional matrix adjust. Identities = 79/167 (47%), Positives = 107/167 (64%), Gaps = 6/167 (3%) Query: 154 ELQHQAHHDVLTGLPNRMMLAERMEAALTHACRERNGVALLFLDLDGFKPINDTLGHKAG 213 EL HD LT LPNR +L +R+ A+ A RE+ AL+F+DLDGFK IND+LGH G Sbjct: 261 ELTQMVLHDQLTRLPNRTLLEDRLNQAINKAAREQGHFALMFMDLDGFKAINDSLGHHVG 320 Query: 214 DQVLQEVTRRLRQVARHGDTLARVGGDEFVLLITDLGQPYENGAQVLAEKCIRVVSEPLH 273 D++L EV +RL +R DT+AR+GGDEFV+L+ DL P + A +AEK + V++P Sbjct: 321 DRLLVEVAQRLTSSSRANDTVARLGGDEFVILV-DLNTPED--AMGVAEKLVEAVNQPFK 377 Query: 274 LAQGEYQLGVSVGIAVC--DGSCDADSLLQAADKAMYAAKGNGRGCY 318 + Q E ++ SVGIA+ DG+ D L+ AD AMY K +GR Y Sbjct: 378 VDQHELRVSASVGIAIYPEDGATRHD-LVINADAAMYHTKRSGRNGY 423 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 139 bits (351), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 81/198 (40%), Positives = 120/198 (60%), Gaps = 8/198 (4%) Query: 132 DEVGHLTMAFNGL---LSRLKLHQAELQHQAHHDVLTGLPNRMMLAERMEAALTHACRER 188 DE G L+ + G ++ K H+AE++HQA HD LTGLPNR ML E ++ AL A ++ Sbjct: 237 DEAGKLS-CYEGTVEAITERKAHEAEMRHQATHDALTGLPNRNMLHEHLQRALRVARQKG 295 Query: 189 NGVALLFLDLDGFKPINDTLGHKAGDQVLQEVTRRLRQVARHGDTLARVGGDEFVLLITD 248 A+ F+DLD FK IND+LGH+ GD++L+ V +RL+ R D +AR GGDEFVL++ + Sbjct: 296 ALTAVAFVDLDQFKFINDSLGHQVGDELLKTVAQRLQACLREADMVARQGGDEFVLVLQN 355 Query: 249 LGQPYENGAQVLAEKCIRVVSEPLHLAQGEYQLGVSVGIAV--CDGSCDADSLLQAADKA 306 + A+V+ ++ + V+ P E+Q+ S+G++ DG D +SLL+ AD A Sbjct: 356 QTEGEAGIAEVM-QRILAAVARPWQAGDREFQVTASIGVSRYPADGK-DVESLLKHADSA 413 Query: 307 MYAAKGNGRGCYVIAPAW 324 MY AK GR + W Sbjct: 414 MYRAKEQGRNNFQFFAPW 431 >gi|300312026|ref|YP_003776118.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1106 Score = 139 bits (350), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 76/166 (45%), Positives = 105/166 (63%), Gaps = 5/166 (3%) Query: 155 LQHQAHHDVLTGLPNRMMLAERMEAALTHACRERNG-VALLFLDLDGFKPINDTLGHKAG 213 ++H AH+D LTGLPNR + + + A+THA RNG VA++ +DLD FK INDT GH+ G Sbjct: 666 IRHLAHYDELTGLPNRFLCTQHVSNAITHA-EHRNGMVAVILMDLDRFKNINDTFGHETG 724 Query: 214 DQVLQEVTRRLRQVARHGDTLARVGGDEFVLLITDLGQPYENGAQVLAEKCIRVVSEPLH 273 + VLQE+ +R R R D LARVGGDEF++L+ D P + G +A+K + +P Sbjct: 725 EAVLQEIAQRFRNSLRELDILARVGGDEFIVLVDDFDDPVQLGE--IAQKLLTEARKPFI 782 Query: 274 LAQGEYQLGVSVGIAVCDGSCD-ADSLLQAADKAMYAAKGNGRGCY 318 + E L VS+GIA G D A +L++ AD AMY AK +G+ Y Sbjct: 783 IDGQEAMLSVSIGIATYPGDGDNAQTLIKNADIAMYRAKHHGKDDY 828 >gi|300310189|ref|YP_003774281.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 706 Score = 138 bits (348), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 74/166 (44%), Positives = 107/166 (64%), Gaps = 3/166 (1%) Query: 154 ELQHQAHHDVLTGLPNRMMLAERMEAALTHACRERNGVALLFLDLDGFKPINDTLGHKAG 213 +L H A HDVLTGLPNR +LA+R++ A+ A R A+LF+DLDGFK IND+LGH G Sbjct: 257 KLLHFATHDVLTGLPNRALLADRIQHAVEVARRNGKTFAVLFMDLDGFKAINDSLGHAVG 316 Query: 214 DQVLQEVTRRLRQVARHGDTLARVGGDEFVLLITDLGQPYENGAQVLAEKCIRVVSEPLH 273 D +L V +R+RQ R D +AR+GGDEFV+++ +L P + + L+E + + + Sbjct: 317 DGLLVAVAQRIRQCLRGEDMVARIGGDEFVVVVGNLSSP--DVVEQLSENILAALRQDFQ 374 Query: 274 LAQGEYQLGVSVGIAVCDGSCDA-DSLLQAADKAMYAAKGNGRGCY 318 + ++ S+GIAV S D+ D+L++ AD AMY AK +GR Y Sbjct: 375 IDDATLRVTSSIGIAVYPNSGDSVDALMKNADAAMYEAKQSGRNTY 420 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,116,505 Number of extensions: 47121 Number of successful extensions: 283 Number of sequences better than 1.0e-08: 38 Number of HSP's gapped: 228 Number of HSP's successfully gapped: 38 Length of query: 349 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 263 Effective length of database: 1,212,660 Effective search space: 318929580 Effective search space used: 318929580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)