BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0080.p (701 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312090|ref|YP_003776182.1| ATP-dependent DNA helicase [Her... 1219 0.0 gi|300309775|ref|YP_003773867.1| ATP-dependent DNA helicase [Her... 208 1e-54 gi|300313605|ref|YP_003777697.1| ATP-dependent DNA helicase rep ... 201 1e-52 >gi|300312090|ref|YP_003776182.1| ATP-dependent DNA helicase [Herbaspirillum seropedicae SmR1] Length = 707 Score = 1219 bits (3154), Expect = 0.0, Method: Compositional matrix adjust. Identities = 610/676 (90%), Positives = 637/676 (94%) Query: 1 MPPELAQDVPDTSSPPTGERDPFARLNAQQREAVEHGSKMAPAQQPPLLIVAGAGSGKTN 60 MPPEL QD PDT+S P E DPFARLNAQQREAVEHG K+ P+QQPPLLIVAGAGSGKTN Sbjct: 1 MPPELIQDAPDTTSLPDRESDPFARLNAQQREAVEHGGKLPPSQQPPLLIVAGAGSGKTN 60 Query: 61 TLAHRVARLILDGADPQRILLLTFSRRAAADMGWRVGQVIRQVLGLAATDSPPQLPWSGT 120 TLAHRVARL+L+GADPQRILLL+FSRRAAADMG RVGQVI QVLGLAA+D+PPQL WSGT Sbjct: 61 TLAHRVARLVLEGADPQRILLLSFSRRAAADMGRRVGQVIAQVLGLAASDTPPQLHWSGT 120 Query: 121 FHAIGARLLREYAAVIGLEEDFTIQDRGDSEDLIGLLRHEMGLSSSDKRFPLKGTCLSIY 180 FHAIGARLLREYA VIGLEEDFTIQDRGDSEDL+GLLRH+MGLS+++KRFPLK TCL+IY Sbjct: 121 FHAIGARLLREYAKVIGLEEDFTIQDRGDSEDLMGLLRHDMGLSATEKRFPLKATCLAIY 180 Query: 181 SRAVNTRQDLGTVLQTQFPWCREWERLLGELFTRYVEAKEAQHVLDYDDLLLFWADMLSD 240 SRAVNTRQDLG VLQ FPWC EWER LGELF RYV+AKE QHVLDYDDLLLFWADML D Sbjct: 181 SRAVNTRQDLGEVLQQHFPWCCEWERPLGELFGRYVDAKEEQHVLDYDDLLLFWADMLGD 240 Query: 241 HEIAQSVGARFDHVLVDEYQDTNRLQAEILQRMKPDGAGVTAVGDDAQSIYSFRGATVRN 300 +IAQSVG RFDHVLVDEYQDTNRLQAEILQRMKPDGAGVTAVGDDAQ+IYSFRGATVRN Sbjct: 241 AQIAQSVGQRFDHVLVDEYQDTNRLQAEILQRMKPDGAGVTAVGDDAQAIYSFRGATVRN 300 Query: 301 ILDFPELFTQPARLLTLEQNYRSTQPILQASNAVIADAPERHVKQLWSDKPSSQKPQLVV 360 ILDFP LFTQP RLLTLEQNYRSTQPIL ASNAVIADAPERHVK LWSDKPSSQKPQLVV Sbjct: 301 ILDFPGLFTQPVRLLTLEQNYRSTQPILHASNAVIADAPERHVKHLWSDKPSSQKPQLVV 360 Query: 361 IPDEAEQARWVANRILAHREEGLTLKSQAVLFRTATHSATLELELVRRNIPFVKFGGLKF 420 IPDEAEQARWVANRILAHREEGLTLKSQAVLFRTATHSA LELEL RRNIPFVKFGGLKF Sbjct: 361 IPDEAEQARWVANRILAHREEGLTLKSQAVLFRTATHSAALELELARRNIPFVKFGGLKF 420 Query: 421 LESTHIKDLLSLLRLAHNPSGRMAGFRALQLIPGVGPATAKKVLDLMAAASDPADALLNF 480 LES HIKDLL+LLRLAHNPSGRMAG RALQLIPGVGPATA+KVLDLMAAA+DPADALLNF Sbjct: 421 LESGHIKDLLALLRLAHNPSGRMAGLRALQLIPGVGPATARKVLDLMAAAADPADALLNF 480 Query: 481 GAPASIALQWQAFADTYDLMRRASASWPTDIQAARDWYLPQLEQRYEDADARAADIEQLT 540 GAPA+IALQWQAFADTY LMRRASASWPTDI AARDWYLPQL++RY+DA ARAADIEQL Sbjct: 481 GAPANIALQWQAFADTYALMRRASASWPTDILAARDWYLPQLQERYDDAQARAADIEQLA 540 Query: 541 ALANGYASREKFLTELTLDPPEASSDFSGPPYRDEDYLTLSTIHSAKGQEWRSVHVLNVV 600 LANGYASREKFLTELTLDPPEASSDFSGPP RDEDYLTLSTIHSAKGQEWRSVH+LNVV Sbjct: 541 VLANGYASREKFLTELTLDPPEASSDFSGPPLRDEDYLTLSTIHSAKGQEWRSVHILNVV 600 Query: 601 DGCIPSDLATGSEAEIAEERRLLYVAMTRAKEYLHLVVPQRFYITQQTAQGDRHVNGIRS 660 DGCIPSDLATGSEAEIAEERRLLYVAMTRA++YLHLVVPQRFYITQQT QGDRHVNGIRS Sbjct: 601 DGCIPSDLATGSEAEIAEERRLLYVAMTRARQYLHLVVPQRFYITQQTPQGDRHVNGIRS 660 Query: 661 RFITPRMLPFFEDSLW 676 RFITPRMLP FEDSLW Sbjct: 661 RFITPRMLPHFEDSLW 676 >gi|300309775|ref|YP_003773867.1| ATP-dependent DNA helicase [Herbaspirillum seropedicae SmR1] Length = 759 Score = 208 bits (530), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 205/681 (30%), Positives = 308/681 (45%), Gaps = 100/681 (14%) Query: 42 PAQQPPLLIVAGAGSGKTNTLAHRVARLILDG-ADPQRILLLTFSRRAAADMGWRV-GQV 99 PAQ LI+AGAGSGKT L R+A LI G P IL +TF+ +AA +M R+ G + Sbjct: 19 PAQSA--LILAGAGSGKTRVLTTRIAWLIQTGQVSPSGILAVTFTNKAAKEMTARLSGML 76 Query: 100 IRQVLGLAATDSPPQLPWSGTFHAIGARLLREYAAVIGLEEDFTIQDRGDSEDLIGLLRH 159 G+ W GTFH + RLLR + GL + F I D D I L Sbjct: 77 PINTRGM----------WIGTFHGLCNRLLRAHHKDAGLPQTFQILDTQDQLSAIKRLLK 126 Query: 160 EMGLSSSDKRFPLKGTCLSIYSRAVNTRQDLGTVLQTQFPWCREWERLLGELFTRYVEAK 219 +M + D+++P + I N+ +D G + + ++ R EL+ Y + Sbjct: 127 QMNVD--DEKYPPRNLMYFI-----NSAKDQGLRAKDVDAY-DDYNRKFVELYELYDQQC 178 Query: 220 EAQHVLDYDDLLLFWADMLSDHE-IAQSVGARFDHVLVDEYQDTNRLQAEILQRMKPDGA 278 + + V+D+ +LLL ++LS ++ + + +RF H+LVDE+QDTN LQ + L+ M Sbjct: 179 QREGVVDFAELLLRTYELLSRNQPLREHYQSRFRHILVDEFQDTNDLQYKWLKLMAGQHN 238 Query: 279 GVTAVGDDAQSIYSFRGATVRNILDFPELFTQPARLLTLEQNYRSTQPILQASNAVIADA 338 V AVGDD QSIY+FRGA V N+ F + F L+ LEQNYRS IL +N +IA+ Sbjct: 239 AVFAVGDDDQSIYAFRGANVGNMQAFEQEF-HVQNLIKLEQNYRSHGHILDTANELIANN 297 Query: 339 PERHVKQLWSDKPSSQKPQLVVIPDEAEQARWVANRILAHREEGLTLKSQAVLFRTATHS 398 +R K L +D ++ ++ + ++A+W+ ++ EG A+L+R+ S Sbjct: 298 SKRLGKNLRTDAGHGEQVRVYEATSDLQEAQWIIEQVKDLIAEGWARSEIAILYRSNAQS 357 Query: 399 ATLELELVRRNIPFVKFGGLKFLESTHIKDLLSLLRLAHNPSGRMAGFRALQL-IPGVGP 457 LE L IP+ +GG +F E IK ++ L+L N A R + G+G Sbjct: 358 RVLEHALFSSAIPYRVYGGQRFFERAEIKHAIAYLQLMDNLHNDSAFLRVVNFPTRGIG- 416 Query: 458 ATAKKVLDLMAAASDPADALLNFGAPASIALQ----WQAFADTYDLMRRASASWPTDIQA 513 AK + L AA +L + A +A + AF + R + P Sbjct: 417 --AKAMESLQDAARQYGCSL--YAAVPYVAGKAGSSLNAFVKLIEGARFETQHLPLPEMV 472 Query: 514 ARDWYLPQLEQRY----EDADARAADIEQLTALANGYASREKFLTELTLDPPEASSDFSG 569 L L Y E AD R ++EQL + A + S E + LD P F G Sbjct: 473 KVVLDLSGLMTHYRNEKEGAD-RIENLEQLVSAATLFVSEEGY----GLDAPA----FLG 523 Query: 570 PPYR-----------------------------------------------DEDYLTLST 582 P + +D L L T Sbjct: 524 PQAQATAGAAITTADGVEVLDADAPLVTIMSPLSAFLSHASLEAGDNQAQAGQDALQLMT 583 Query: 583 IHSAKGQEWRSVHVLNVVDGCIPSDLATGSEAEIAEERRLLYVAMTRAKEYLHLVVPQRF 642 +HSAKG E+ +V + + +G P + + E + EERRL+YVA+TRA++ L + Q Sbjct: 584 VHSAKGLEFDAVFITGMEEGLFPHENSAKEEGGVEEERRLMYVAITRARKRLFMTFSQTR 643 Query: 643 YITQQTAQGDRHVNGIRSRFI 663 + QT +RSRF Sbjct: 644 MLHGQTRY------NMRSRFF 658 >gi|300313605|ref|YP_003777697.1| ATP-dependent DNA helicase rep [Herbaspirillum seropedicae SmR1] Length = 690 Score = 201 bits (512), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 206/669 (30%), Positives = 325/669 (48%), Gaps = 98/669 (14%) Query: 22 PFARLNAQQREAVEHGSKMAPAQQPPLLIVAGAGSGKTNTLAHRVARLILD-GADPQRIL 80 P LN Q EAV + MA P L++AGAGSGKT + ++A LI + G + + I Sbjct: 4 PSFGLNKPQSEAVHY---MAG----PCLVLAGAGSGKTRVITQKIAHLIENCGYESRNIA 56 Query: 81 LLTFSRRAAADMGWRVGQVIRQVLGLAATDSPPQLPW--SGTFHAIGARLLREYAAVIGL 138 LTF+ +AA +M R+ ++++ P Q TFH++G ++LR+ + +GL Sbjct: 57 ALTFTNKAALEMQERIAKLLK---------DPKQAKHLTVSTFHSLGVKILRQESQHLGL 107 Query: 139 EEDFTIQDRGDSEDLIGLLRHEMGLSSSDKRFPLKGTCLSIYSRAVNTRQDLGTVLQTQF 198 ++ F+I D D L+ E+ +++ +S++ + DL + Sbjct: 108 KDRFSIMDSDDCFSLV----QELAVTTDKAIIRGVQNSISLWKNGL-VDPDLA-----EK 157 Query: 199 PWCREWERLLGELFTRYVEAKEAQHVLDYDDLLLFWADML-SDHEIAQSVGARFDHVLVD 257 E E ++ YV +A +D+DDL+ ++ S+ E+ + ++L+D Sbjct: 158 NARTEDEAQAARIYRSYVATLKAYQAVDFDDLIRLPVELFRSNEEVRDRWQRKLRYLLID 217 Query: 258 EYQDTNRLQAEILQRMKPDGAG----VTAVGDDAQSIYSFRGATVRNI----LDFPELFT 309 EYQDTN Q E++ ++ G G TAVGDD Q+IY++RGAT+ N+ +DFP L Sbjct: 218 EYQDTNTCQYELV-KLLVTGIGKKPMFTAVGDDDQAIYAWRGATIENLKLLGVDFPNL-- 274 Query: 310 QPARLLTLEQNYRSTQPILQASNAVIADAPERHVKQLWSDKPSSQKPQLVVIPDEAEQAR 369 L+ LEQNYRS+ ILQA+NAVI++ P+ K LWS+ ++ + DE +A Sbjct: 275 ---HLIKLEQNYRSSTRILQAANAVISNNPKLFDKTLWSEHGLGDPITVMAMDDEEAEAD 331 Query: 370 WVANRILAHR-EEGLTLKSQAVLFRTATHSATLELELVRRNIPFVKFGGLKFLESTHIKD 428 +A + AHR E A+L+R + LE L R IP+V GG + + IKD Sbjct: 332 QIAITLSAHRFERRAKFADYAILYRGNHQARILEKSLRRERIPYVMSGGQSYFDRAEIKD 391 Query: 429 LLSLLRLAHNPSGRMAGFRALQLIP--GVGPATAKKVLDLMAAASDPA--DALLNFGAPA 484 +++ LRL N A RA+ P GVG AT +VL +A + +A+ G Sbjct: 392 IIAYLRLIANQDDDPAFIRAVT-TPRRGVGQATL-EVLGTLAGQWQCSLFEAVYKGGLED 449 Query: 485 SIA----LQWQAFADTYD-LMRRASASWPT-----------DIQAARDW--YLPQLEQRY 526 + L + F D + L RA+ P+ D+ A ++ YL Y Sbjct: 450 KLTDRQLLPLRKFCDFINALESRATRPGPSGSGENAGKVLDDMMEAINYEFYL------Y 503 Query: 527 EDADARAADI--EQLTALANGYASR----------EKFLTELTLDPPEASSDFSGPPYRD 574 + + RAA + + N R EK L E+T + + S RD Sbjct: 504 DSFEERAAQARWQNVVDFINWLKERACGGRDGEGEEKNLLEIT----QMVALMSMLEGRD 559 Query: 575 E--DYLTLSTIHSAKGQEWRSVHVLNVVDGCIP--SDLAT---GSEAEIAEERRLLYVAM 627 E D + +ST+H++KG E+ V ++ V +G +P D T + A + EERRL+YV + Sbjct: 560 EEQDAVRMSTLHASKGLEFPHVFLVGVEEGILPHKGDPDTPPEEAAARVEEERRLMYVGI 619 Query: 628 TRAKEYLHL 636 TRA+ LHL Sbjct: 620 TRAQRSLHL 628 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,312,325 Number of extensions: 101698 Number of successful extensions: 358 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 348 Number of HSP's successfully gapped: 4 Length of query: 701 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 609 Effective length of database: 1,184,250 Effective search space: 721208250 Effective search space used: 721208250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)