BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0066.p (219 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312076|ref|YP_003776168.1| signal transduction histidine k... 169 1e-43 gi|300311282|ref|YP_003775374.1| transmembrane sensor histidine ... 96 2e-21 gi|300311886|ref|YP_003775978.1| multi-sensor signal transductio... 93 1e-20 gi|300310534|ref|YP_003774626.1| transmembrane sensor histidine ... 90 1e-19 gi|300312939|ref|YP_003777031.1| transmembrane nitrate/nitrite s... 68 6e-13 >gi|300312076|ref|YP_003776168.1| signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 356 Score = 169 bits (429), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 116/220 (52%), Positives = 136/220 (61%), Gaps = 3/220 (1%) Query: 1 MSDPAAPALSAAFRIREEERTRIARDLHDELGAQLTGIGMALGQLREQLALERHAA-LPQ 59 MS P P + A IRE+ER RIARDLHD+LG L G+GMALG+L+EQLA + AA L + Sbjct: 137 MSHPLPPP-ATAIHIREQERERIARDLHDDLGGVLAGVGMALGRLQEQLAAQGLAAPLAE 195 Query: 60 ADYAAQLLAQAREGMERIIDDLHPPIVEFGLADALRWQCRHFSRQSGVDCQFLATDQLAV 119 AD A QLL QA M IID+LHPPIVEFGL DAL+WQCR R+ + CQ L Sbjct: 196 ADEARQLLQQAMASMHSIIDELHPPIVEFGLVDALQWQCRQIQREYRLHCQLDCPPTLEQ 255 Query: 120 QDVFLILSLLRILREALNNIARHAAASQVEVRVEQTGDSLHLHIDDNGRGFDPA-ATGRD 178 +D FLIL LLRILREA +N ARH AS + V + L + I D+G G P A Sbjct: 256 EDRFLILGLLRILREAASNAARHGRASALRVDLRMRQARLEMQIADDGCGIAPLHAKEVA 315 Query: 179 GRRGHGLANMRQRAAALGGSAAWEARAGHGSRVHICIPVA 218 R GHGL NMR+RA ALGG EA G + IPVA Sbjct: 316 PRHGHGLRNMRERAQALGGELFIEAGQDGGVVIRTLIPVA 355 >gi|300311282|ref|YP_003775374.1| transmembrane sensor histidine kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 464 Score = 95.9 bits (237), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 77/198 (38%), Positives = 102/198 (51%), Gaps = 19/198 (9%) Query: 14 RIREEERTRIARDLHDELGAQLTGIGM----ALGQLR---EQLALERHAALPQADYAAQL 66 R +EEER R++RDLHD + L I + AL +L EQ A R A + Sbjct: 244 RSQEEERARLSRDLHDGISQWLVSIKLQIEAALVRLNGTPEQAATARAGFEKTATQINGV 303 Query: 67 LAQAREGMERIIDDLHPPIVE-FGLADALRWQCRHFSRQSGVDCQFL------ATDQLAV 119 LA+ R RI DL P I++ GLA AL C + +G + + A D + Sbjct: 304 LAEVR----RISHDLRPAILDDLGLAAALEHLCGEWRDAAGAGVEIVFENQAQADDGATL 359 Query: 120 QDVFLILSLLRILREALNNIARHAAASQVEVRVEQTGDSLHLHIDDNGRGFDPAATGRDG 179 DV + L RI +EAL NIARHA AS+++VR+ Q + L L + DNG GFD Sbjct: 360 PDVVNTV-LFRIAQEALTNIARHAGASRIKVRLYQGHNLLMLQVQDNGHGFDLGQVDDHS 418 Query: 180 RRGHGLANMRQRAAALGG 197 R G GL NMR+R A+GG Sbjct: 419 RHGIGLRNMRERIEAVGG 436 >gi|300311886|ref|YP_003775978.1| multi-sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 93.2 bits (230), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 68/214 (31%), Positives = 111/214 (51%), Gaps = 11/214 (5%) Query: 10 SAAFRIREEERTRIARDLHDELGAQLTGIGMALGQLREQLALERHAALPQADYAAQLLAQ 69 S ++E ER +AR LHD++G LT + + L +++ +LE + D +LAQ Sbjct: 262 SRVIEVQERERQHLARGLHDDVGQSLTALKINLETIKKMGSLEGPSLQNGIDIVTAVLAQ 321 Query: 70 AREGMERIIDDLHPPIVE-FGLADALRWQCRHFSRQSGVDCQFLAT--DQLAVQDVFLIL 126 R + DL PP ++ GL ALR S+ + V+ F ++ D+ D+ Sbjct: 322 VRS----LSLDLRPPQLDNLGLIAALRSYVEQKSKLANVNGWFESSGHDEQLHHDIEN-- 375 Query: 127 SLLRILREALNNIARHAAASQVEVRVEQTGDSLHLHIDDNGRGFDPAATGRDGRRG--HG 184 + RI++EA+ NI RHA + V++++ D ++L I D+GRGFD + G G Sbjct: 376 TAFRIVQEAVTNILRHARCQNIWVKIDRQHDQVYLSIRDDGRGFDIERARSNAISGTSFG 435 Query: 185 LANMRQRAAALGGSAAWEARAGHGSRVHICIPVA 218 L NM +RA +GGS + G G+ + + +PVA Sbjct: 436 LLNMEERAVLVGGSMEITSAPGAGTEIVMHLPVA 469 >gi|300310534|ref|YP_003774626.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 677 Score = 90.1 bits (222), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 63/207 (30%), Positives = 105/207 (50%), Gaps = 10/207 (4%) Query: 15 IREEERTRIARDLHDELGAQLTGIGMALGQLREQLALERHAA---LPQADYAAQLLAQAR 71 +REEE+ IAR+LHD +GA LT + M L + + L + A + L+A R Sbjct: 468 VREEEKRAIARELHDNMGASLTALSMHLEGVYQILPPDEKWADRKVRMQGLMKSLVATTR 527 Query: 72 EGMERIIDDLHPPIVE-FGLADALRWQCRHFSRQSGVDCQFLATDQLAVQDVFLILSLLR 130 RI +L P ++ FGL A+ Q ++G+ C D+ + +++ R Sbjct: 528 ----RIQTELRPNTLDLFGLKAAISEQLDELHERTGIACHASLPDEDVEVGHEMEIAIYR 583 Query: 131 ILREALNNIARHAAASQVEVRVEQTGDSLHLHIDDNGRGFDPAATGRDGRRGHGLANMRQ 190 +L+E LNN+ +HA AS+V+V ++ D + L + D+G G RD + +GL +R+ Sbjct: 584 MLQEMLNNVTKHAKASKVDVILDIDEDHVALTVRDDGVGI--PEERRDNHKTYGLRGLRE 641 Query: 191 RAAALGGSAAWEARAGHGSRVHICIPV 217 RA GG + G G+ + I +P+ Sbjct: 642 RATFFGGEVDIRSTPGKGALITISLPI 668 >gi|300312939|ref|YP_003777031.1| transmembrane nitrate/nitrite sensor histidine kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 650 Score = 68.2 bits (165), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 61/207 (29%), Positives = 100/207 (48%), Gaps = 20/207 (9%) Query: 19 ERTRIARDLHDELGAQLTGIGMALGQLREQLALERHAALPQADYAAQLLAQAREGMERII 78 ER +A+ LHD + L + + + L + LA +R L + A LL R G++ Sbjct: 446 ERNLLAQGLHDSIAQGLNFLNLQVQMLEDSLARDR---LDEIRDIAPLL---RTGVQENY 499 Query: 79 DDLHPPIVEF-------GLADALRWQCRHFSRQSGVDCQFLATDQ---LAVQDVFLILSL 128 +D+ ++ F L +R RQSG+ Q T LA + L + Sbjct: 500 EDVRELLLNFRTRLQDSNLDSEMRKALDKLQRQSGLQGQITLTGDGPPLAPEQQ---LQV 556 Query: 129 LRILREALNNIARHAAASQVEVRVEQTGDSLHLHIDDNGRGFDPAATGRDGRRGHGLANM 188 L IL+EAL+NI +HA A QV++ V+ D + + D+G GF + G GL M Sbjct: 557 LFILQEALSNIRKHARAQQVQISVDNRQD-FRMTVSDDGHGFTLESARAKGLEHVGLRIM 615 Query: 189 RQRAAALGGSAAWEARAGHGSRVHICI 215 ++RA LG + +++ GHG+R+ + + Sbjct: 616 QERAERLGANLEIDSQPGHGTRIALTL 642 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.136 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 587,400 Number of extensions: 20867 Number of successful extensions: 121 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 115 Number of HSP's successfully gapped: 5 Length of query: 219 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 137 Effective length of database: 1,231,600 Effective search space: 168729200 Effective search space used: 168729200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 128 (53.9 bits)