BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0063.p (546 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312035|ref|YP_003776127.1| methyl-accepting chemotaxis tra... 891 0.0 gi|300313312|ref|YP_003777404.1| methyl-accepting chemotaxis pro... 382 e-107 gi|300312219|ref|YP_003776311.1| methyl-accepting chemotaxis tra... 344 1e-95 gi|300310474|ref|YP_003774566.1| methyl-accepting chemotaxis tra... 339 3e-94 gi|300312448|ref|YP_003776540.1| methyl-accepting chemotaxis tra... 330 2e-91 >gi|300312035|ref|YP_003776127.1| methyl-accepting chemotaxis transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 548 Score = 891 bits (2303), Expect = 0.0, Method: Compositional matrix adjust. Identities = 493/551 (89%), Positives = 515/551 (93%), Gaps = 8/551 (1%) Query: 1 MTITKRLILTLSTALLALLFVGADGLWQLQRAQQRLELIQNRIIPGMDSLNLLKGYLADS 60 MTITKRLI+TLSTALLALLFVGADGLWQLQRAQQRLELIQNRIIPGMDSLNLLKGYLADS Sbjct: 1 MTITKRLIITLSTALLALLFVGADGLWQLQRAQQRLELIQNRIIPGMDSLNLLKGYLADS 60 Query: 61 RLAGYRLSVFANLPDKTALQKAVDASNKALDDEFAHYERDLIFDEKDRALIQADKANIEA 120 RLAGYRLSVFANL DK+ LQKAVD +NK LD+EFAHYERDLIFDEKDRALIQADKANIEA Sbjct: 61 RLAGYRLSVFANLSDKSGLQKAVDTANKLLDEEFAHYERDLIFDEKDRALIQADKANIEA 120 Query: 121 YRKALVPFFAAAYANDMDGVRATLQAGTPLAVSAAAAKKGFEDHILVGRQMIDTVKQESD 180 YRKALVPFFAAAYANDMDGVRATLQAGTPLA+SAAAAKKGFEDHIL+GR+MIDT+KQESD Sbjct: 121 YRKALVPFFAAAYANDMDGVRATLQAGTPLAISAAAAKKGFEDHILIGRKMIDTIKQESD 180 Query: 181 AAYTFALRAMIAVIVVAVLLTGVLGIRTLRSVTRSLGKIQQTFEHVSQTLDLSRPVPVER 240 AY FALR MIAV+VVAVLLTG+LG++ +RSV RSLGKIQ TFEHVSQTLDLSRPVPVER Sbjct: 181 EAYAFALRTMIAVVVVAVLLTGLLGLQVVRSVIRSLGKIQHTFEHVSQTLDLSRPVPVER 240 Query: 241 MDEIGRTAAAFNKLLARIVDVVATTRTSTDSVTVAARQIAAGNADLSARTEQQAAALEES 300 MDEIGRTAAAFNKLLARIVDVVATTRTSTDSVTVAARQIAAGNADLSARTEQQAAALEES Sbjct: 241 MDEIGRTAAAFNKLLARIVDVVATTRTSTDSVTVAARQIAAGNADLSARTEQQAAALEES 300 Query: 301 ASSLEELTSVVLQNTQNARQANELAQSASHIASQGGDVVRQVVDTMNSINASSRKIVDII 360 ASSLE+LTSVVLQNTQNARQANELAQSASHIASQGGDVVRQVVDTMNSIN SSRKIVDII Sbjct: 301 ASSLEQLTSVVLQNTQNARQANELAQSASHIASQGGDVVRQVVDTMNSINESSRKIVDII 360 Query: 361 AVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKELIDDSV 420 AVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKELIDDSV Sbjct: 361 AVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKELIDDSV 420 Query: 421 DKVGSGSKLVEQAGATMSDIVDSVQRVTQIVGNIADASEEQSAGITQVNQAISQMDQGTQ 480 KVGSGSKLVEQAG TMSDIVDSVQRVT IVG+IA+ASEEQSAGI+QVNQAI+QMDQGTQ Sbjct: 421 SKVGSGSKLVEQAGTTMSDIVDSVQRVTVIVGDIANASEEQSAGISQVNQAIAQMDQGTQ 480 Query: 481 QNAALVEEAAAAAQALQGQAEKLESVVAVFKLEGR--AAMPVRSVEVKPVAAARV---PV 535 QNAALVEEAAAAA ALQ QAE LESVVAVFKLEGR ++PV P +AAR+ P Sbjct: 481 QNAALVEEAAAAAHALQQQAETLESVVAVFKLEGRLNGSIPVIQA---PASAARLPASPA 537 Query: 536 ASGQVPELEEF 546 A P LEEF Sbjct: 538 ARQAEPALEEF 548 >gi|300313312|ref|YP_003777404.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 544 Score = 382 bits (981), Expect = e-107, Method: Compositional matrix adjust. Identities = 231/515 (44%), Positives = 323/515 (62%), Gaps = 10/515 (1%) Query: 1 MTITKRLILTLSTALLALLFVGADGLWQLQRAQQRLELIQNRIIPGMDSLNLLKGYLADS 60 ++I RL + + ++ + + + ++ R ++P + SL+ + + Sbjct: 3 LSIVNRLYILIGAVIIGCVVLAGANMVKMNRTYADASFASENVVPSLLSLSTITQGINGM 62 Query: 61 RLAGYRLSVFANLPDKTALQKAVDASNKALDDEFAHYERDLIFDEKDRALIQADKANIEA 120 R+A +R D+ A++ + +++ K ++D YE++ + D D+ L++A + + + Sbjct: 63 RIATWRYLALTESVDRGAIESSFNSAKKRVEDALQKYEQEQLADATDKELLRAVRVSYQE 122 Query: 121 YRKALVPFFAAAYANDMDGVRATLQAGTPLA---VSAAAAKKGFEDHILVGRQMIDTVKQ 177 + K+L A A + RA L P+ A + + + I Q Q Sbjct: 123 FDKSLAEALKLANAGQWNEARALLANNQPVIDRLNKAMTELRAYNEKI---SQAGAHRAQ 179 Query: 178 ESDAAYTFALRAMIAVIVVAVLLTGVLGIRTLRSVTRSLGKIQQTFEHVSQTLDLSRPVP 237 E+ AA + A ++ V L G+L R + RSL + + E +++ DL+R + Sbjct: 180 ETMAAARLHAILISAAVIAVVTLMGLL---IARKIARSLAEAVELAETIAEG-DLTREID 235 Query: 238 VERMDEIGRTAAAFNKLLARIVDVVATTRTSTDSVTVAARQIAAGNADLSARTEQQAAAL 297 DEIG AA K+ +V +VAT RT ++V A+ QIA GN DLSARTEQQA++L Sbjct: 236 TSSTDEIGHLMAAMAKMNRNLVTIVATVRTGVNTVAAASSQIATGNMDLSARTEQQASSL 295 Query: 298 EESASSLEELTSVVLQNTQNARQANELAQSASHIASQGGDVVRQVVDTMNSINASSRKIV 357 E++A++ EELTS V QN +NA QA++LA +AS IA++GG+VV+QVV TM I +SS I Sbjct: 296 EQTAAATEELTSTVKQNAENALQADQLASAASGIAAEGGEVVKQVVTTMEEIRSSSLNIA 355 Query: 358 DIIAVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKELID 417 DII+VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVRSLAQRSA+AAKEIK+LID Sbjct: 356 DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSASAAKEIKQLID 415 Query: 418 DSVDKVGSGSKLVEQAGATMSDIVDSVQRVTQIVGNIADASEEQSAGITQVNQAISQMDQ 477 DSV KV +GS+LV AG TM +V+SV+RVT +V I+ AS EQS GI QVNQAIS MDQ Sbjct: 416 DSVSKVENGSQLVMSAGNTMQQVVESVRRVTDVVTEISAASREQSLGIEQVNQAISHMDQ 475 Query: 478 GTQQNAALVEEAAAAAQALQGQAEKLESVVAVFKL 512 TQ+NAALVEEA AAAQ+LQ QA +LE V VFKL Sbjct: 476 STQENAALVEEATAAAQSLQEQARQLERAVNVFKL 510 >gi|300312219|ref|YP_003776311.1| methyl-accepting chemotaxis transducer transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 563 Score = 344 bits (882), Expect = 1e-95, Method: Compositional matrix adjust. Identities = 228/522 (43%), Positives = 319/522 (61%), Gaps = 11/522 (2%) Query: 1 MTITKRLILTLSTALLALLFVGADGLWQLQRAQQRLELIQNRIIPGMDSLNLLKGYLADS 60 MTI ++L L + +L ++ GL+Q R IP + +LN A Sbjct: 31 MTIARKLYLLIVAVVLGVIMQAVVGLYQSHRIASSAGYSATDTIPSVLALNG-----ATD 85 Query: 61 RLAGYRLSVFANLPDKTALQKAVD---ASNKALDDEFAHYERDLIFDEKDRALIQADKAN 117 + G RL++ + + TA +K V A ++ D F +ER I D+ DR +++AD+ Sbjct: 86 AIYGIRLNIARYVIEPTAREKTVANMAALHQKAQDFFDQFERCCISDDTDRRMLEADRRF 145 Query: 118 IEAYRKALVPFFAAAYANDMDGVRATLQAGTPLAVSAAAAKKGFEDHILVGRQMIDTVKQ 177 Y K + A D +R ++ +A + +H Q+ + Sbjct: 146 FAEYEKHRMAMVDLGSAGKEDALRVAME--EKMAPTGQRLMAVLNEHKNYNEQLGRQGAE 203 Query: 178 ESDAAYTFALRAMIAVIVVAVLLTGVLGIRTLRSVTRSLGKIQQTFEHVSQTLDLSRPVP 237 +D ++ IA + +++ L + +R++ SL + + + + DLS + Sbjct: 204 AADEHLQLSIYLTIAAATLVIVMVSALSLLLVRNIRHSLHAATELADTIGRG-DLSSQIV 262 Query: 238 VERMDEIGRTAAAFNKLLARIVDVVATTRTSTDSVTVAARQIAAGNADLSARTEQQAAAL 297 + DE+ + + ++ +V ++ S ++ A R+IA+GN DLS+RTE QA +L Sbjct: 263 LRGRDEVTQLMLSLQRMTGSLVQILGKVNDSAATIETATREIASGNLDLSSRTEAQAGSL 322 Query: 298 EESASSLEELTSVVLQNTQNARQANELAQSASHIASQGGDVVRQVVDTMNSINASSRKIV 357 EE+ S++ +LT+ V QN NARQA+ELAQSAS +A Q G VV +V+ TM +INASS KIV Sbjct: 323 EETVSAMGQLTTTVKQNADNARQADELAQSASGVAGQAGKVVDEVILTMEAINASSLKIV 382 Query: 358 DIIAVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKELID 417 DII+VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSA+AA+EIKELID Sbjct: 383 DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAREIKELID 442 Query: 418 DSVDKVGSGSKLVEQAGATMSDIVDSVQRVTQIVGNIADASEEQSAGITQVNQAISQMDQ 477 DSV+KV SGS+LV QAG+TM ++V SV+RV+ IV +I+ AS EQS GI QVNQAI MDQ Sbjct: 443 DSVNKVQSGSRLVLQAGSTMEEVVTSVRRVSGIVRDISVASGEQSDGIAQVNQAIGMMDQ 502 Query: 478 GTQQNAALVEEAAAAAQALQGQAEKLESVVAVFKLEGRAAMP 519 TQQNAALVEEAAAA+Q+L+ QA+ L V+VF+LE A P Sbjct: 503 TTQQNAALVEEAAAASQSLREQAKGLAQAVSVFRLERSAEAP 544 >gi|300310474|ref|YP_003774566.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 339 bits (870), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 237/519 (45%), Positives = 318/519 (61%), Gaps = 16/519 (3%) Query: 1 MTITKRLILTLSTALLALLFVGADGLWQLQRAQQRLELIQNRIIPGMDSLNLLKGYLADS 60 + I K+L L+ + + L G + +L++ + I ++ +P MD+ L+G L Sbjct: 7 LNIAKKLALSFAVVIALTLLQGLFAIKELKQVNKASGEIVSKWMPTMDAARELQGSLPPL 66 Query: 61 RLAGYRLSVFANLPDKTALQKAVDASNKALDDEFAHYERDLIFDEKDRALIQADKANIEA 120 RL L ++ A KA+ AL + A +E+ + ++ Q K N+ A Sbjct: 67 RLNELELVTAVTPQERAAANKAIKDRLDALTRQRAIFEQRMSEPQEKEQYAQFLK-NLTA 125 Query: 121 YRKALVPFFAAAYANDMDGVRATLQAGTPLAVSAAAAKKGFEDHILVGRQMIDT-----V 175 Y K A D RA + + G D I+ I+T Sbjct: 126 YVKVDEQLAEMAANQQYDETRALFNGDSAKLYRSMV---GNLDAIV----KINTSGSAQA 178 Query: 176 KQESDAAYTFALRAMIAVIVVAVLLTGVLGIRTLRSVTRSLGKIQQTFEHVSQTLDLS-R 234 +E+ + A R ++ ++ VL+ +L +V+R L + Q + V++ DL+ R Sbjct: 179 DEEASTVFRGAQRWIVGLLAATVLIASLLAFLVATNVSRPLKEAVQIAQRVARG-DLTLR 237 Query: 235 PVPVERMDEIGRTAAAFNKLLARIVDVVATTRTSTDSVTVAARQIAAGNADLSARTEQQA 294 P R DE G A + + D+V+ R TD++T A+ +IA GN DLS RTEQQA Sbjct: 238 IRPAGR-DETGALMEALRAMNESLRDIVSEVRQGTDTITTASHEIARGNLDLSNRTEQQA 296 Query: 295 AALEESASSLEELTSVVLQNTQNARQANELAQSASHIASQGGDVVRQVVDTMNSINASSR 354 +LEE+AS++EELTS V QN NARQAN+LA +AS IASQGG+VV QVV TM I SSR Sbjct: 297 GSLEETASAMEELTSTVTQNADNARQANQLAATASDIASQGGEVVGQVVQTMEGITESSR 356 Query: 355 KIVDIIAVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKE 414 +I DII+VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSA AAKEIKE Sbjct: 357 RIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAGAAKEIKE 416 Query: 415 LIDDSVDKVGSGSKLVEQAGATMSDIVDSVQRVTQIVGNIADASEEQSAGITQVNQAISQ 474 LIDDSV KV +GS LVE+AG+TM+++V SV+RVT +V I+ A++EQS GI +VN AI+Q Sbjct: 417 LIDDSVQKVSAGSALVERAGSTMTEVVASVRRVTDVVAEISAATQEQSGGINEVNLAITQ 476 Query: 475 MDQGTQQNAALVEEAAAAAQALQGQAEKLESVVAVFKLE 513 MD+ TQQNAALVE+AAAAA +L+GQA L +VV FKL+ Sbjct: 477 MDETTQQNAALVEQAAAAADSLKGQASNLAAVVGKFKLD 515 >gi|300312448|ref|YP_003776540.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 596 Score = 330 bits (845), Expect = 2e-91, Method: Compositional matrix adjust. Identities = 203/300 (67%), Positives = 241/300 (80%) Query: 231 DLSRPVPVERMDEIGRTAAAFNKLLARIVDVVATTRTSTDSVTVAARQIAAGNADLSART 290 DL+ V V +EIGR +A + ++ V+ R+S+DS+ +A++IAAGN DLS+RT Sbjct: 234 DLTTKVEVASSNEIGRLFSAIRSMQQALLTTVSRVRSSSDSIDTSAKEIAAGNMDLSSRT 293 Query: 291 EQQAAALEESASSLEELTSVVLQNTQNARQANELAQSASHIASQGGDVVRQVVDTMNSIN 350 EQQA++LEE+A+S+EELT V QN +NA QAN+LA SAS AS+GG+VV QVVDTM +IN Sbjct: 294 EQQASSLEETAASMEELTGTVKQNAENALQANQLAHSASSTASKGGEVVSQVVDTMQAIN 353 Query: 351 ASSRKIVDIIAVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAK 410 SSRKIVDII+VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAK Sbjct: 354 DSSRKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAK 413 Query: 411 EIKELIDDSVDKVGSGSKLVEQAGATMSDIVDSVQRVTQIVGNIADASEEQSAGITQVNQ 470 EIK LIDDSV+KV +GS+LVEQAGATMS++V SVQRVT IVG IA+AS EQS GI QVN+ Sbjct: 414 EIKSLIDDSVEKVEAGSQLVEQAGATMSEVVTSVQRVTDIVGEIAEASREQSTGIEQVNR 473 Query: 471 AISQMDQGTQQNAALVEEAAAAAQALQGQAEKLESVVAVFKLEGRAAMPVRSVEVKPVAA 530 AI+QMD+ TQQNAALVEEAAAAAQ+LQ QA L V++FK+E P KP AA Sbjct: 474 AITQMDEVTQQNAALVEEAAAAAQSLQAQATNLVGAVSIFKIEAHQVAPAGKPASKPRAA 533 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.127 0.329 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,072,915 Number of extensions: 34668 Number of successful extensions: 440 Number of sequences better than 1.0e-08: 71 Number of HSP's gapped: 263 Number of HSP's successfully gapped: 73 Length of query: 546 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 456 Effective length of database: 1,193,720 Effective search space: 544336320 Effective search space used: 544336320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)