BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0061.p (116 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312033|ref|YP_003776125.1| hypothetical protein Hsero_2721... 178 1e-46 >gi|300312033|ref|YP_003776125.1| hypothetical protein Hsero_2721 [Herbaspirillum seropedicae SmR1] Length = 113 Score = 178 bits (451), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 100/116 (86%), Positives = 103/116 (88%), Gaps = 3/116 (2%) Query: 1 MIARIVRLICLSLAAMAMLGSAVQASASDLLTFKLEMQDGKLIPPRIEVPAGQRIKIEVH 60 MIAR VRL L+ A M +A QA AS+L TFKLEMQDGKLIPPRIEVPAGQRIKIEVH Sbjct: 1 MIARTVRLALLAAALMG---AAAQAPASELPTFKLEMQDGKLIPPRIEVPAGQRIKIEVH 57 Query: 61 NIGKSAAEFESVELRKEKVLAPGAQSFVVIAPLRPGEYKFFDDFHLNMPPGVIVAK 116 NIGKSAAEFESVELRKEKVLAPGAQSFVVIAPLRPGEYKFFDDFHLNMP GVIVAK Sbjct: 58 NIGKSAAEFESVELRKEKVLAPGAQSFVVIAPLRPGEYKFFDDFHLNMPQGVIVAK 113 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.138 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 309,453 Number of extensions: 9994 Number of successful extensions: 24 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 23 Number of HSP's successfully gapped: 1 Length of query: 116 Length of database: 1,619,870 Length adjustment: 73 Effective length of query: 43 Effective length of database: 1,274,215 Effective search space: 54791245 Effective search space used: 54791245 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 124 (52.4 bits)