BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0055.p (498 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312026|ref|YP_003776118.1| signal transduction protein con... 959 0.0 gi|300311453|ref|YP_003775545.1| signal transduction containing ... 404 e-113 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 397 e-111 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 385 e-108 gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 377 e-105 >gi|300312026|ref|YP_003776118.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1106 Score = 959 bits (2479), Expect = 0.0, Method: Compositional matrix adjust. Identities = 461/497 (92%), Positives = 480/497 (96%) Query: 2 LIDVEIYPSCGAWPIMGKEGQLLGVFAALLKENQAPSNEYLQLANIAADLASVAIESRRA 61 LID EIYPSCGAWPI+GKEGQLLGVFAALLKENQAPSNEYLQLANIAADLASVAIESRRA Sbjct: 603 LIDTEIYPSCGAWPIIGKEGQLLGVFAALLKENQAPSNEYLQLANIAADLASVAIESRRA 662 Query: 62 DERIRHLAHYDELTGLPNRFLCTQHVSNAITHAEHRNGMVAVLLMDLDRFKNINDTFGHE 121 DERIRHLAHYDELTGLPNRFLCTQHVSNAITHAEHRNGMVAV+LMDLDRFKNINDTFGHE Sbjct: 663 DERIRHLAHYDELTGLPNRFLCTQHVSNAITHAEHRNGMVAVILMDLDRFKNINDTFGHE 722 Query: 122 AGEAVLQEIAQRFRNTLRELDILARVGGDEFIVLVDDFDDPLQLGEIAQKLLTEARKPFI 181 GEAVLQEIAQRFRN+LRELDILARVGGDEFIVLVDDFDDP+QLGEIAQKLLTEARKPFI Sbjct: 723 TGEAVLQEIAQRFRNSLRELDILARVGGDEFIVLVDDFDDPVQLGEIAQKLLTEARKPFI 782 Query: 182 IDGQEAMLSASIGIATYPGDGDNAQTLIKNADIAMYRAKHHGKDDYRFFSDEVNTNTVER 241 IDGQEAMLS SIGIATYPGDGDNAQTLIKNADIAMYRAKHHGKDDYRFFSDE+NTNTVER Sbjct: 783 IDGQEAMLSVSIGIATYPGDGDNAQTLIKNADIAMYRAKHHGKDDYRFFSDEINTNTVER 842 Query: 242 IALEAELRRAIERAEFVVHYQPKINLATGGIVGAEALVRWQHPVRGLVPPGEFIPLAEET 301 IALEAELRRAIERAEFVVHYQPKI LA G IVGAEALVRWQHP+RGL+PPGEFI LAEET Sbjct: 843 IALEAELRRAIERAEFVVHYQPKIELAEGRIVGAEALVRWQHPLRGLLPPGEFITLAEET 902 Query: 302 RLIDQIGLVVLDVACRDIGLMLERELVCGRISINLTGSQFGDEHLLEDIKSVVQRHGTPP 361 RLIDQIGL+VLDVACRDIGLM ++++ CGRISINLTGSQFGDE LL+DIK VV RHGTPP Sbjct: 903 RLIDQIGLLVLDVACRDIGLMRQQQMTCGRISINLTGSQFGDERLLDDIKEVVDRHGTPP 962 Query: 362 ACLEFEITESMVMHNRDRAIAIMDGIRAEGFTISIDDFGTGYSSLAYLKRFPVNTLKIDK 421 +CLEFEITESMVMHNR+RAIAIMDGIRAEGFTISIDDFGTGYSSLAYLKRFPVNTLKIDK Sbjct: 963 SCLEFEITESMVMHNRERAIAIMDGIRAEGFTISIDDFGTGYSSLAYLKRFPVNTLKIDK 1022 Query: 422 SFINDIPDDANSSAIVQAIIAMSHALNLKVVTEGVETEKQLQALREFGSDEYQGYYFSKP 481 SFINDIPD+ NSSAIVQAIIAMSHALNLKVVTEGVETEKQLQALREFGSDEYQGYYFSKP Sbjct: 1023 SFINDIPDNPNSSAIVQAIIAMSHALNLKVVTEGVETEKQLQALREFGSDEYQGYYFSKP 1082 Query: 482 VPYAQFLQMLVTEGDPP 498 VPYAQF+Q+L + D P Sbjct: 1083 VPYAQFVQLLAGQQDRP 1099 >gi|300311453|ref|YP_003775545.1| signal transduction containing PAS/GGDEF/EAL domains protein [Herbaspirillum seropedicae SmR1] Length = 683 Score = 404 bits (1037), Expect = e-113, Method: Compositional matrix adjust. Identities = 221/441 (50%), Positives = 290/441 (65%), Gaps = 9/441 (2%) Query: 57 ESRRADERIRHLAHYDELTGLPNRFLCTQHVSNAITHAEHRNGMVAVLLMDLDRFKNIND 116 E + A++ I HLA+YD LTGLPNR L T+ V A+ A H N V+ +DL+RFK IND Sbjct: 232 ERKNAEKLIHHLAYYDALTGLPNRTLLTRTVDQALEEARHENLHGCVMFIDLNRFKPIND 291 Query: 117 TFGHEAGEAVLQEIAQRFRNTLRELDILARVGGDEFIVLV--DDFDDPLQLGEIAQKLLT 174 T GH AG+ +L E+A R R LRE D++AR+G DEF V + D D P +AQKLL Sbjct: 292 TLGHVAGDMLLVEVAMRLRRALREQDVVARLGADEFAVALFGIDKDSP---AYVAQKLLA 348 Query: 175 EARKPFIIDGQEAMLSASIGIATYPGDGDNAQTLIKNADIAMYRAKHHGKDD---YRFFS 231 PF IDG E + ASIG++ YP + + +TL++ ADIAMYRAK G+++ Y +S Sbjct: 349 LFDDPFFIDGHELRVGASIGVSLYPENATDTETLLRLADIAMYRAKQGGENNEGGYACYS 408 Query: 232 DEVNTNTVERIALEAELRRAIERAEFVVHYQPKINLATGGIVGAEALVRWQHPVRGLVPP 291 +E+N NT++ + +E L A E E +++YQPK+++ T I GAEALVRWQHP RGL+ P Sbjct: 409 EEMNRNTLDLLRIETGLLHAFEYNELLLYYQPKVDMQTLAITGAEALVRWQHPERGLLLP 468 Query: 292 GEFIPLAEETRLIDQIGLVVLDVACRDIGLMLERELVCGRISINLTGSQFGDEHLLEDIK 351 GEFIP+AEET LI Q+ VLD CR + L RI++N+T +F L + + Sbjct: 469 GEFIPIAEETGLIVQLSDWVLDAVCRQARAWKDAGLPPIRIALNVTAREF-TRSLPDRVG 527 Query: 352 SVVQRHGTPPACLEFEITESMVMHNRDRAIAIMDGIRAEGFTISIDDFGTGYSSLAYLKR 411 + ++RH LE EITESM+MH+ DR I+IM+ I G T+S+DDFGTGYSSL+YLKR Sbjct: 528 AALERHMLSGEWLELEITESMLMHSTDRVISIMEKICGLGITVSLDDFGTGYSSLSYLKR 587 Query: 412 FPVNTLKIDKSFINDIPDDANSSAIVQAIIAMSHALNLKVVTEGVETEKQLQALREFGSD 471 FP+NTLKID+SF IPDD N AI AII ++ L KV+ EGVE EKQ LRE G + Sbjct: 588 FPINTLKIDRSFTMGIPDDTNDCAIASAIIGIAKQLRHKVIAEGVEDEKQFNFLREAGCN 647 Query: 472 EYQGYYFSKPVPYAQFLQMLV 492 E QGY FS+PVP F ML+ Sbjct: 648 ELQGYIFSRPVPAPDFHAMLL 668 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 397 bits (1019), Expect = e-111, Method: Compositional matrix adjust. Identities = 196/438 (44%), Positives = 288/438 (65%), Gaps = 10/438 (2%) Query: 59 RRADERIRHLAHYDELTGLPNRFLCTQHVSNAITHAEHRNGMVAVLLMDLDRFKNINDTF 118 +R + + A D LTGL NR + H+ AI AE + + V+ +DLDRFK +NDT Sbjct: 509 KRYEAELEFQASRDTLTGLINRHMLRHHLEQAIGVAERQKQALWVVFVDLDRFKFVNDTL 568 Query: 119 GHEAGEAVLQEIAQRFRNTLRELDILARVGGDEFIVLVDDFDDPLQLGEIAQKLLTEARK 178 GH+AG+ +L+ +A R R +RE D +AR+GGDEFI+++ + D+ Q+++ Sbjct: 569 GHKAGDTLLRTVAGRLRAAVREADTVARLGGDEFIMILPEQDELTLDTRSLQRIMDAVAA 628 Query: 179 PFIIDGQEAMLSASIGIATYPGDGDNAQTLIKNADIAMYRAKHHGKDDYRFFSDEVNTNT 238 P + G ++ S+G+A YP DG + +TLIK+ADIAMYR K G+++++F++ +N Sbjct: 629 PVTLGGHTLFMTCSVGVACYPNDGTDGETLIKHADIAMYRGKQTGRNNFQFYTPAMNEQA 688 Query: 239 VERIALEAELRRAIERAEFVVHYQPKINLATGGIVGAEALVRWQHPVRGLVPPGEFIPLA 298 +ER+ +E +LR A++ EF++HYQPK++LA+G ++G+EAL+RWQHP G+V P FI LA Sbjct: 689 LERLRIEGDLRNALDHGEFLLHYQPKVDLASGRVIGSEALIRWQHPQLGMVSPARFIGLA 748 Query: 299 EETRLIDQIGLVVLDVACRDIGLMLERELVCG-----RISINLTGSQFGDEHLLEDIKSV 353 EET LI IG VL AC R+ G +S+NL+ QF + L+ I V Sbjct: 749 EETGLILPIGAWVLRAACTQC-----RQWQLGGYADISVSVNLSVGQFLQKDLVSSIADV 803 Query: 354 VQRHGTPPACLEFEITESMVMHNRDRAIAIMDGIRAEGFTISIDDFGTGYSSLAYLKRFP 413 ++ G PP LE E+TES++M + + AI I+ G++ G +SIDDFGTGYSSLAYLKRFP Sbjct: 804 LEDTGLPPESLELELTESLMMTDVEHAIGILAGLKKLGVRLSIDDFGTGYSSLAYLKRFP 863 Query: 414 VNTLKIDKSFINDIPDDANSSAIVQAIIAMSHALNLKVVTEGVETEKQLQALREFGSDEY 473 ++ LKID+SF+ DI DA+ +AI +II+++ L L+V+ EGVET++QL LR +G DE Sbjct: 864 IDVLKIDRSFVRDITIDADDAAITASIISLARHLKLRVIAEGVETDEQLDYLRHYGCDEI 923 Query: 474 QGYYFSKPVPYAQFLQML 491 QGY FS PVP +F Q+L Sbjct: 924 QGYLFSPPVPADKFEQIL 941 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 385 bits (989), Expect = e-108, Method: Compositional matrix adjust. Identities = 192/435 (44%), Positives = 293/435 (67%), Gaps = 3/435 (0%) Query: 55 AIESRRADE-RIRHLAHYDELTGLPNRFLCTQHVSNAITHAEHRNGMVAVLLMDLDRFKN 113 AI R+A E +RH A +D LTGLPNR + +H+ A+ A + + AV +DLD+FK Sbjct: 251 AITERKAHEAEMRHQATHDALTGLPNRNMLHEHLQRALRVARQKGALTAVAFVDLDQFKF 310 Query: 114 INDTFGHEAGEAVLQEIAQRFRNTLRELDILARVGGDEFI-VLVDDFDDPLQLGEIAQKL 172 IND+ GH+ G+ +L+ +AQR + LRE D++AR GGDEF+ VL + + + E+ Q++ Sbjct: 311 INDSLGHQVGDELLKTVAQRLQACLREADMVARQGGDEFVLVLQNQTEGEAGIAEVMQRI 370 Query: 173 LTEARKPFIIDGQEAMLSASIGIATYPGDGDNAQTLIKNADIAMYRAKHHGKDDYRFFSD 232 L +P+ +E ++ASIG++ YP DG + ++L+K+AD AMYRAK G+++++FF+ Sbjct: 371 LAAVARPWQAGDREFQVTASIGVSRYPADGKDVESLLKHADSAMYRAKEQGRNNFQFFAP 430 Query: 233 EVNTNTVERIALEAELRRAIERAEFVVHYQPKINLATGGIVGAEALVRWQHPVRGLVPPG 292 +++ R+ + LRRA+++ EF ++YQPK++L G ++GAEAL+RW+ P +G+VPP Sbjct: 431 WMDSQVSNRLEMLINLRRALDQDEFKLYYQPKLSLQDGRLIGAEALIRWESPQQGMVPPD 490 Query: 293 EFIPLAEETRLIDQIGLVVLDVACRDIGLMLERELVCGRISINLTGSQFGDEHLLEDIKS 352 FIP AEE LI IG VL ACR + L +++NL+ Q ++ L + + Sbjct: 491 RFIPFAEEAGLIVPIGEWVLRTACRQNQAWQQAGLPPIPVAVNLSPRQL-NQSLPDFVAG 549 Query: 353 VVQRHGTPPACLEFEITESMVMHNRDRAIAIMDGIRAEGFTISIDDFGTGYSSLAYLKRF 412 V+ + G + LE EITE++VM + ++++A + ++ G IS+DDFGTGYSSL+YL+RF Sbjct: 550 VLAQSGLAASWLELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGYSSLSYLRRF 609 Query: 413 PVNTLKIDKSFINDIPDDANSSAIVQAIIAMSHALNLKVVTEGVETEKQLQALREFGSDE 472 PV+ LKIDKSF+ DI DA+S+AIV+AII+++H LNL+V+ EGVE E+Q L+E DE Sbjct: 610 PVDALKIDKSFVRDIVRDADSAAIVKAIISLAHILNLRVIAEGVEDEEQHAFLKENACDE 669 Query: 473 YQGYYFSKPVPYAQF 487 QGYYF KP+ A F Sbjct: 670 VQGYYFGKPMEVAVF 684 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 377 bits (967), Expect = e-105, Method: Compositional matrix adjust. Identities = 196/447 (43%), Positives = 272/447 (60%), Gaps = 1/447 (0%) Query: 45 ANIAADLASVAIESRRADERIRHLAHYDELTGLPNRFLCTQHVSNAITHAEHRNGMVAVL 104 A + + A +A A+E + + +D+LT LPNR L ++ AI A G A++ Sbjct: 242 ARLESRTAKLARSLAAANEELTQMVLHDQLTRLPNRTLLEDRLNQAINKAAREQGHFALM 301 Query: 105 LMDLDRFKNINDTFGHEAGEAVLQEIAQRFRNTLRELDILARVGGDEFIVLVDDFDDPLQ 164 MDLD FK IND+ GH G+ +L E+AQR ++ R D +AR+GGDEF++LVD + P Sbjct: 302 FMDLDGFKAINDSLGHHVGDRLLVEVAQRLTSSSRANDTVARLGGDEFVILVD-LNTPED 360 Query: 165 LGEIAQKLLTEARKPFIIDGQEAMLSASIGIATYPGDGDNAQTLIKNADIAMYRAKHHGK 224 +A+KL+ +PF +D E +SAS+GIA YP DG L+ NAD AMY K G+ Sbjct: 361 AMGVAEKLVEAVNQPFKVDQHELRVSASVGIAIYPEDGATRHDLVINADAAMYHTKRSGR 420 Query: 225 DDYRFFSDEVNTNTVERIALEAELRRAIERAEFVVHYQPKINLATGGIVGAEALVRWQHP 284 + Y FF +N N +++ +LR A+ER +F + YQPK +L G ++GAEAL+RW+HP Sbjct: 421 NGYHFFEPSMNANAQQQLQWLQDLRVALERGQFRLVYQPKFSLPAGPVLGAEALLRWEHP 480 Query: 285 VRGLVPPGEFIPLAEETRLIDQIGLVVLDVACRDIGLMLERELVCGRISINLTGSQFGDE 344 V GLV P +FI LAE + LI IG VLD ACR + V +S+NL+ QF D Sbjct: 481 VHGLVAPDQFIGLAERSGLILPIGAWVLDEACRQMREWFALGYVDWTMSVNLSALQFADP 540 Query: 345 HLLEDIKSVVQRHGTPPACLEFEITESMVMHNRDRAIAIMDGIRAEGFTISIDDFGTGYS 404 HLLE +++ ++RH PP CL E+TES MH+ + ++ + I G ISIDDFGTGYS Sbjct: 541 HLLEVVRTTLERHQLPPPCLTLEVTESTAMHDTEASLQTLRQIADLGVEISIDDFGTGYS 600 Query: 405 SLAYLKRFPVNTLKIDKSFINDIPDDANSSAIVQAIIAMSHALNLKVVTEGVETEKQLQA 464 SL YLKR P N LKID+ F+ ++ D +AIV AI+A+ LNL++V EGVET +Q Sbjct: 601 SLLYLKRLPANELKIDRGFVRELRQDGEDAAIVSAIVALGRTLNLRIVAEGVETVEQQDF 660 Query: 465 LREFGSDEYQGYYFSKPVPYAQFLQML 491 L G D QGY +P+ FLQ + Sbjct: 661 LTAAGCDALQGYLMGRPMTPELFLQTM 687 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,517,297 Number of extensions: 62451 Number of successful extensions: 274 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 210 Number of HSP's successfully gapped: 45 Length of query: 498 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 409 Effective length of database: 1,198,455 Effective search space: 490168095 Effective search space used: 490168095 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)