BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0053.p (368 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312025|ref|YP_003776117.1| bifunctional glutathione-depend... 755 0.0 gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase... 238 6e-64 gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldeh... 98 1e-21 gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 97 2e-21 gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herb... 74 2e-14 >gi|300312025|ref|YP_003776117.1| bifunctional glutathione-dependent formaldehyde dehydrogenase/alcohol dehydrogenase class III oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 368 Score = 755 bits (1949), Expect = 0.0, Method: Compositional matrix adjust. Identities = 368/368 (100%), Positives = 368/368 (100%) Query: 1 MKTRAAVAWQAGKPLTIEEVELGGPKAGEVLVEIKATGICHTDYYTLSGADPEGIFPAIL 60 MKTRAAVAWQAGKPLTIEEVELGGPKAGEVLVEIKATGICHTDYYTLSGADPEGIFPAIL Sbjct: 1 MKTRAAVAWQAGKPLTIEEVELGGPKAGEVLVEIKATGICHTDYYTLSGADPEGIFPAIL 60 Query: 61 GHEGAGVVVDVGPGVKSLKKDDHVIPLYTPECRECKFCLSQKTNLCQAIRATQGRGLMPD 120 GHEGAGVVVDVGPGVKSLKKDDHVIPLYTPECRECKFCLSQKTNLCQAIRATQGRGLMPD Sbjct: 61 GHEGAGVVVDVGPGVKSLKKDDHVIPLYTPECRECKFCLSQKTNLCQAIRATQGRGLMPD 120 Query: 121 ATSRFSLDGKPLFHYMGTSTFSNYIVVPEIALAKIRSDAPFDKVCYIGCGVTTGVGAVLF 180 ATSRFSLDGKPLFHYMGTSTFSNYIVVPEIALAKIRSDAPFDKVCYIGCGVTTGVGAVLF Sbjct: 121 ATSRFSLDGKPLFHYMGTSTFSNYIVVPEIALAKIRSDAPFDKVCYIGCGVTTGVGAVLF 180 Query: 181 TAKVEAGANVVVFGLGGIGLNVIQAAKMVGANKIIGVDLNPAREAMARKFGMTDFINPKD 240 TAKVEAGANVVVFGLGGIGLNVIQAAKMVGANKIIGVDLNPAREAMARKFGMTDFINPKD Sbjct: 181 TAKVEAGANVVVFGLGGIGLNVIQAAKMVGANKIIGVDLNPAREAMARKFGMTDFINPKD 240 Query: 241 VPNVVDAIIGMTDGGADYSFECIGNTTTMRQSLECCHKGWGQSIIIGVAAAGQEISTRPF 300 VPNVVDAIIGMTDGGADYSFECIGNTTTMRQSLECCHKGWGQSIIIGVAAAGQEISTRPF Sbjct: 241 VPNVVDAIIGMTDGGADYSFECIGNTTTMRQSLECCHKGWGQSIIIGVAAAGQEISTRPF 300 Query: 301 QLVTGRVWKGSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHKLPLERINEGFDLMKSGE 360 QLVTGRVWKGSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHKLPLERINEGFDLMKSGE Sbjct: 301 QLVTGRVWKGSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHKLPLERINEGFDLMKSGE 360 Query: 361 SIRSVVEF 368 SIRSVVEF Sbjct: 361 SIRSVVEF 368 >gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 374 Score = 238 bits (607), Expect = 6e-64, Method: Compositional matrix adjust. Identities = 135/377 (35%), Positives = 203/377 (53%), Gaps = 12/377 (3%) Query: 1 MKTRAAV--------AWQAGKPLTIEEVELGGPKAGEVLVEIKATGICHTDYYTLSGADP 52 MK +AAV + +PL IEE++L P GEVLV +KA G+CH+D ++G P Sbjct: 1 MKIQAAVLREMSDAHPFAVSQPLKIEELDLDPPGPGEVLVRMKAAGLCHSDLSVITGVRP 60 Query: 53 EGIFPAILGHEGAGVVVDVGPGVKSLKKDDHVIPLYTPECRECKFCLSQKTNLCQAIRAT 112 + P LGHE + VV+VG GV L+ D V+ ++ P C C C+ + LC+ AT Sbjct: 61 RPV-PMALGHEASAEVVEVGSGVNDLRAGDLVVLVFVPSCGHCLPCMEGRPALCEPGAAT 119 Query: 113 QGRGLMPDATSRFSLDGKPLFHYMGTSTFSNYIVVPEIALAKIRSDAPFDKVCYIGCGVT 172 G + R +P+ H++G S F+++ VV + KI +D + GC V Sbjct: 120 NTVGELLSGQRRLRDKTQPVHHHLGVSAFADHAVVSRRSCVKIEADIDPVQAALFGCAVL 179 Query: 173 TGVGAVLFTAKVEAGANVVVFGLGGIGLNVIQAAKMVGANKIIGVDLNPAREAMARKFGM 232 TGVGA + TA+V+AG V GLGG+GL + A GA ++I VDL+ ++ A+AR G Sbjct: 180 TGVGAAVNTAEVKAGTTAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALARSLGA 239 Query: 233 TDFINPKDVPNVVDAIIGMTDGGADYSFECIGNTTTMRQSLECCHKGWGQSIIIGVAAAG 292 T +N +D P+ V+ + +T GG DY+FE G+ M + +G G ++ G+ A G Sbjct: 240 TATVNARD-PDAVEKVRALTRGGVDYAFEMAGSVQAMEAAYRMTRRG-GMTVTAGLPAPG 297 Query: 293 QEISTRPFQLVT-GRVWKGSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHKLPLERINE 351 Q + + LV R KGS G D+P+ + + GKL +D L+ +L L IN Sbjct: 298 QHWALQQVSLVAEERTVKGSYIGSCVPVRDIPRYIGLFQAGKLPVDRLMGERLALADINR 357 Query: 352 GFDLMKSGESIRSVVEF 368 GFD + GE +R ++ F Sbjct: 358 GFDRLHGGEGLRDLIVF 374 >gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 98.2 bits (243), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 106/369 (28%), Positives = 162/369 (43%), Gaps = 37/369 (10%) Query: 26 KAGEVLVEIKATGICHTDYYTLSGADPEGIFPAILGHEGAGVVVDVGPGVKSLKKDDHVI 85 +A +V++++ AT IC +D + G P ILGHE GVVV+ GP +K LKK D V+ Sbjct: 24 EADDVILKVTATAICGSDLHLYRGKIPATKAGDILGHEFMGVVVEAGPAIKRLKKGDRVV 83 Query: 86 PLYTPECRECKFCLSQKTNLCQAIRATQGRGLMPDATSRFSLDGKPLFHYMGT-----ST 140 + C +C FC C+ +G LM R G +F Y Sbjct: 84 VPFVIACGQCFFCDQHLYAACETTNPDRG-ALMNAKGIR---SGAAMFGYSHLYGGMPGG 139 Query: 141 FSNYIVVPEIALAKIRSDAPF--DKVCYIGCGVTTGVGAVLFTAKVEAGANVVVFGLGGI 198 + Y+ VP+ + IR D+ ++V ++ + TG AV A V G++V ++G G + Sbjct: 140 QAEYVRVPKADVGPIRIDSALADEQVLFLSDILPTGYQAVK-NAGVREGSSVAIYGAGPV 198 Query: 199 GLNVIQAAKMVGANKIIGVDLNPAREAMARKFGMTDFINPKDVPNVVDAIIGMTDG-GAD 257 G +A+M+GA +I VD + R A++ +N V + + II T G G D Sbjct: 199 GQMAAASARMLGAERIFMVDHHAYRLRFAQEQYDVIPVNFDQV-DPAEYIIANTAGRGVD 257 Query: 258 -----YSFECIGNTTTMRQSLECCHKGWGQSIIIGVAAA--GQEISTRPFQLVTGRVWKG 310 FE G+ + GQ++ +A+ G IS V G Sbjct: 258 GVIDAVGFEAKGSVLETAMTTLKLEGSSGQALRHCIASVRRGGVISVPG---VYAGFIHG 314 Query: 311 SAFGG--------ARGRTDV----PKIVDWYMEGKLNIDDLITHKLPLERINEGFDLMKS 358 FG G+T V P+++ EG+L D +ITH+LPLE G+ + Sbjct: 315 FLFGDIFEKGVTVKTGQTHVQHYMPELLASIEEGRLRPDAIITHRLPLEDAARGYQIFND 374 Query: 359 G-ESIRSVV 366 E R VV Sbjct: 375 KLEDCRKVV 383 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 97.4 bits (241), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 100/361 (27%), Positives = 166/361 (45%), Gaps = 39/361 (10%) Query: 4 RAAVAWQAGKPLTIEEVELGGPKAGEVLVEIKATGICHTDYYTLSGADP-EGIFPAILGH 62 +AAV + GKPL+IE+V + P G++LV+ +A+G+CHTD + G P + P I GH Sbjct: 6 KAAVVREFGKPLSIEQVPVPTPAPGQILVKFEASGVCHTDLHAAHGDWPVKPTPPFIPGH 65 Query: 63 EGAGVVVDVGPGVKSLKKDDHV-IPLYTPECRECKFCLSQKTNLCQAIRATQGRGLMPDA 121 EG G V VG GVK +K+ D V +P C C C + LC + T Sbjct: 66 EGTGYVAAVGAGVKHVKEGDRVGVPWLHTACGCCSPCRTGWETLCAEQQNTG-------- 117 Query: 122 TSRFSLDGKPLFHYMGTSTFSNYIVVPEIALAKIRSDAPFDKVCYIGC-GVTTGVGAVLF 180 +S++G +F+ Y + + + + F + C GVT G L Sbjct: 118 ---YSVNG----------SFAEYGLADPKFVGHLPDNLDFGPAAPVLCAGVTVYKG--LK 162 Query: 181 TAKVEAGANVVVFGLGGIGLNVIQAAKMVGANKIIGVDLNPAREAMARKFGMTDFINPKD 240 +V G VV+ G+GG+G +Q AK +G + ++ D++ + A+A+K G ++ ++ Sbjct: 163 ETEVRPGEWVVISGIGGLGHMAVQYAKAMGMH-VVAADIHEDKLALAKKLGADLTVDGRN 221 Query: 241 VPNVVDAIIGMTDGGADYSFECIGNTTTMRQSLECCHKGWGQSIIIGVAAAGQEISTRPF 300 V + + GGA + + M Q+ + G ++G+ +IS F Sbjct: 222 HNAVAE--VQRIIGGAHGALVTAVSPKAMEQAFGFL-RARGTMALVGLPPG--DISLPVF 276 Query: 301 QLVTGRVW-KGSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHKLPLERINEGFDLMKSG 359 V R+ +GS G R D+ + + + EGK+ L+ IN F M+ G Sbjct: 277 NTVLKRITVRGSIVG---TRQDLEESLVFAAEGKVAAHFTWDK---LDNINAIFARMEEG 330 Query: 360 E 360 + Sbjct: 331 K 331 >gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 73.9 bits (180), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 89/346 (25%), Positives = 143/346 (41%), Gaps = 33/346 (9%) Query: 15 LTIEEVELGGPKAGEVLVEIKATGICHTD--YYTLSGADPEGIF-PAILGHEGAGVVVDV 71 L I+ ++ G + ++ V++KA GIC +D YY G I P +LGHE +G + +V Sbjct: 13 LRIQALDTGALQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMVLGHEVSGQIAEV 72 Query: 72 GPGVKSLKKDDHVIPLYTPECRECKFCLSQKTNLCQAIRATQGRGLMPDATSRFSLDGKP 131 G V + + + C CK+C K N C + RF Sbjct: 73 GTAVTDIAPGTRIAISPSRPCGACKYCQEGKQNHCLDM--------------RFYGSAMR 118 Query: 132 LFHYMGTSTFSNYIVVPEIALAKIRSDAPFDKVCYIGCGVTTGVGAVLFTAKVEAGANVV 191 H G F I++ E + A + +D D + ++ + AV + G V+ Sbjct: 119 TPHVQG--AFRQEIII-ERSQAHVVADHVSDGEAAMAEPLSVALHAVRRAGPL-VGKRVL 174 Query: 192 VFGLGGIGLNVIQAAKMVGANKIIGVDLNPAREAMARKFGMTDFINPKDVPNVVDAIIGM 251 V G G IG V+ AA+ GA +++ D+ A + G IN + P + A Sbjct: 175 VTGCGPIGALVVVAARRAGALEVVVTDVASMPLQAALQVGADSAINMAETPAALKA-YAA 233 Query: 252 TDGGADYSFECIGNTTTMRQSLECCHKGWGQSIIIGVAAAGQEISTRPFQLVTGR--VWK 309 G D FE GN + +LE Q+II+ V G P L+ G+ W+ Sbjct: 234 DKGTFDVLFEASGNERALVGALEALRP---QAIIVQVGLGGD--IQFPANLLVGKELEWR 288 Query: 310 GSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHKLPLERINEGFDL 355 G AF R + V+ +++ LIT P+ + E F++ Sbjct: 289 G-AF---RFHEEFAMAVELMNRKLVDVKPLITATYPIAQAVEAFEI 330 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.139 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,168,053 Number of extensions: 50263 Number of successful extensions: 169 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 164 Number of HSP's successfully gapped: 6 Length of query: 368 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 281 Effective length of database: 1,207,925 Effective search space: 339426925 Effective search space used: 339426925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)