BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0049.p (329 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Her... 639 0.0 gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 107 2e-24 gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 98 8e-22 gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbasp... 94 2e-20 gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspiri... 80 2e-16 >gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 329 Score = 639 bits (1647), Expect = 0.0, Method: Compositional matrix adjust. Identities = 308/329 (93%), Positives = 319/329 (96%) Query: 1 MKSRRFGRTGADVSEIGFGAWAIGGSWGDVSSQDAKAALHAALDAGVTFIDTADVYGDGR 60 MKSRRFGRTGA VSEIGFGAWAIGGSWGDVS+QDAKAALHAALDAGV+FIDTADVYGDGR Sbjct: 1 MKSRRFGRTGAQVSEIGFGAWAIGGSWGDVSAQDAKAALHAALDAGVSFIDTADVYGDGR 60 Query: 61 SEQLIAQVLKERGGARPFVATKAGRRLSPHVASGYNNKDNLTAFVDRSLKNLEVDCLDLV 120 SEQLIAQVLKERGG RPFVATKAGRRLSPHVASGYN++ L+AF+DRSLKNL VDCLDLV Sbjct: 61 SEQLIAQVLKERGGERPFVATKAGRRLSPHVASGYNDRAQLSAFIDRSLKNLGVDCLDLV 120 Query: 121 QLHCPPTEVYYQQEVFAYMDELVAAGKIRHYGVSVEKVEEALKAIEYPNVKSVQIIFNIF 180 QLHCPPTEVYYQQEVF ++D LVAAGKIRHYGVSVEKVEEALKAIEYPNVKSVQIIFNIF Sbjct: 121 QLHCPPTEVYYQQEVFGHLDALVAAGKIRHYGVSVEKVEEALKAIEYPNVKSVQIIFNIF 180 Query: 181 RQRPIGLFLQEAQRRDIAVIARVPLASGLLTGKMNAQTTFAADDHRQFNRHGEAFDVGET 240 RQRPI LFLQEAQRRDIAVIARVPLASGLLTGKM+AQTTFAADDHRQFNRHGEAFDVGET Sbjct: 181 RQRPISLFLQEAQRRDIAVIARVPLASGLLTGKMSAQTTFAADDHRQFNRHGEAFDVGET 240 Query: 241 FSGVPYEVALAAVEKIRALLPQGVPMAQFALRWILMESGISTVIPGARNVAQAQSNSAAS 300 FSGVPYEVALAAVEKIRAL+PQGVPMAQFALRWILMESGISTVIPGARNVAQAQSNSAAS Sbjct: 241 FSGVPYEVALAAVEKIRALVPQGVPMAQFALRWILMESGISTVIPGARNVAQAQSNSAAS 300 Query: 301 ALPPITDEVMQALRELYLHDIAPYVHQRW 329 ALPPIT EVMQALR +YLHDIAPYVHQRW Sbjct: 301 ALPPITAEVMQALRAIYLHDIAPYVHQRW 329 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 107 bits (266), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 92/322 (28%), Positives = 146/322 (45%), Gaps = 22/322 (6%) Query: 3 SRRFGRTGADVSEIGFGAWAIGGSWGDVSSQDAKAALHAALDAGVTFIDTADVYGDGRSE 62 +RR G G VS IG G + + ++ A + AL+ G+ +DTAD+YG +E Sbjct: 17 TRRLGSNGPLVSAIGLGCMGMSDFYAQRDDAESLATIDRALELGINLLDTADMYGPYTNE 76 Query: 63 QLIAQVLKERGGARPFVATKAGRRLSPHVASGYNNKDNLTAF----VDRSLKNLEVDCLD 118 +L+ + +K R + F+ATK G R P G D A+ V+ SLK L V+ +D Sbjct: 77 ELLGRAIKGRRD-KFFIATKFGIRRDPS-DPGARGVDGSPAYIRQAVEGSLKRLGVETID 134 Query: 119 LVQLH--CPPTEVYYQQEVFAYMDELVAAGKIRHYGVSVEKVEEALKAIEYPNVKSVQII 176 L H P T + V A +LV AGKIR+ G+S +A V ++Q Sbjct: 135 LYYQHRIDPATPIEVTMGVLA---DLVQAGKIRYIGLSEASAATLERAHRVHPVTALQSE 191 Query: 177 FNIFRQRPIGLFLQEAQRRDIAVIARVPLASGLLTGKMNAQTTFAADDHRQFNRHGEAFD 236 ++++ + P L + I +A PL G LTG + A DD R+ N Sbjct: 192 YSLWTRDPEAEVLAACRTLGIGFVAYSPLGRGFLTGAIQRFEDLAEDDFRRSN------- 244 Query: 237 VGETFSGVPYEVALAAVEKIRALLPQ-GVPMAQFALRWILMESGISTVIPGARNVAQAQS 295 F G + L EK++ + Q G +Q AL W++ + IPG + + Sbjct: 245 --PRFQGENFARNLQLAEKVKEMAGQHGCTPSQLALAWVMAQDPHIVPIPGTKRRRYLED 302 Query: 296 NSAASALPPITDEVMQALRELY 317 N+ + + + E +QAL ++ Sbjct: 303 NAGSVGV-KLAPEDLQALDAIF 323 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 98.2 bits (243), Expect = 8e-22, Method: Compositional matrix adjust. Identities = 89/316 (28%), Positives = 144/316 (45%), Gaps = 21/316 (6%) Query: 1 MKSRRFGRTGADVSEIGFGAWAIGGSWGDVSSQDAKAALHAALDAGVTFIDTADVYGDGR 60 M RR G++ VS + G+ G Q++ + +A + GV FIDTADVY G+ Sbjct: 1 MHYRRLGKSNLKVSALCLGSMMFGDQ---TDLQESGRIVASAREHGVNFIDTADVYTKGK 57 Query: 61 SEQLIAQVLKERGGARP--FVATKAGRRLSPHVASGYNNKDNLTAFVDRSLKNLEVDCLD 118 SE+++ ++L G R +A+K G +S ++ + + L L+ D LD Sbjct: 58 SEEIVGKLL---AGQRHEWVLASKLGNAMSGKPNESNYSRSWIVRETENILTRLDTDYLD 114 Query: 119 LVQLHCPPTEVYYQQEVFAYMDELVAAGKIRHYGVSV---EKVEEALKAIEYPNVK---S 172 ++ +H + ++ V A + +L+ GKIR +GVS ++ E ++ E NV Sbjct: 115 ILYMHRDYQDENLEEAVQA-LGDLIRVGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPLV 173 Query: 173 VQIIFNIFRQRPIGLFLQEAQRRDIAVIARVPLASGLLTGKMNAQTTFAADDHRQFNRHG 232 Q +N+ ++P L + V+ P+A G+LTGK A D R G Sbjct: 174 CQPYYNLLNRQPEVEILPACAHYGLGVVPYSPIARGVLTGKYQPGQKPAQD-----TRAG 228 Query: 233 EAFDVGETFSGVPYEVALAAVEKIRALLPQGVPMAQFALRWILMESGISTVIPGARNVAQ 292 D + E L A + +GV + QFA W+L S IS+VI G R +AQ Sbjct: 229 RG-DKRMMETEFREESLLIAQRLQQHCEARGVKLGQFATAWVLANSAISSVIAGPRTLAQ 287 Query: 293 AQSNSAASALPPITDE 308 + A+ L +E Sbjct: 288 MEDYYDAAELKISAEE 303 >gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 349 Score = 94.0 bits (232), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 110/362 (30%), Positives = 165/362 (45%), Gaps = 54/362 (14%) Query: 1 MKSRRFGRTGADVSEIGFGAWAIGGSWGDVSSQ-DAKAALHAALDAGVTFIDTADVY--- 56 M+ R GRT VS I G +WG+ +S+ DA A L A++ G+ ID A++Y Sbjct: 1 MQYRPLGRTDLKVSLITLGTM----TWGEQNSEADAHAQLDYAVERGINLIDVAEMYPVP 56 Query: 57 ----GDGRSEQLIAQVLKERGGA-RPFVATKA-GRRLSPH----VASGYNNKDN--LTAF 104 G +E+ + LK+ G + +ATK G PH V G NN D LT Sbjct: 57 PKAETQGLTERYLGTWLKKSGKRDQLLIATKCTGPARKPHNPRHVRGGINNLDRKGLTEA 116 Query: 105 VDRSLKNLEVDCLDLVQLHCPPTEVY------YQ----------QEVFAYMDELVAAGKI 148 + SL+ L++D +DL QLH P V YQ +E + E V AGKI Sbjct: 117 LHGSLERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVKAGKI 176 Query: 149 RHYGVSVEK---VEEALKAIEYPNVKSVQIIFNIFR--QRPIGLFLQE-AQRRDIAVIAR 202 R G+S E V + L+A E ++ + I N + R + L E A R + ++A Sbjct: 177 RTIGLSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVGLLAY 236 Query: 203 VPLASGLLTGKMNAQTTFAADDHRQFNRHGEAFDVGETFSGVPYEVALAAVEKIRALLPQ 262 PLA G+L+GK D R G + E FS A A + AL Q Sbjct: 237 SPLAFGMLSGKY-------LDGARPA---GGRLTLFERFSRYTNAQAERATAEYVALARQ 286 Query: 263 -GVPMAQFALRWILMESGISTVIPGARNVAQAQSNSAASALPPITDEVMQALRELYLHDI 321 G+ AQ AL ++ +++ I GA +AQ +SN ++ L + ++V+ A+ ++ Sbjct: 287 HGLDPAQMALAYVNSRPFLTSNIIGATTLAQLRSNIDSAEL-QLGEDVLAAIEAIHRRQP 345 Query: 322 AP 323 P Sbjct: 346 NP 347 >gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspirillum seropedicae SmR1] Length = 280 Score = 80.1 bits (196), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 82/295 (27%), Positives = 131/295 (44%), Gaps = 56/295 (18%) Query: 8 RTGADVSEIGFGAWAIGGSWGDVSSQDAKAALHAALDAGVTFIDTADVYGDGRSEQLIAQ 67 R G V +G G W +G S +++ AL A LD G++ IDTA++Y +G +E+++A+ Sbjct: 10 RNGQSVPILGQGTWNMGESPAQANAE--VRALQAGLDLGMSLIDTAEMYAEGGAERIVAK 67 Query: 68 VLKERGGARPFVATKAGRRLSPHVASGYNNKDNLTAFVDRSLKNLEVDCLDLVQLHCPPT 127 + R V+ ++ PH AS K A + SLK L D +DL LH Sbjct: 68 AIAGRRDQVYLVS-----KVLPHNAS----KRGTIAACEASLKRLGTDRIDLYLLHW--R 116 Query: 128 EVYYQQEVFAYMDELVAAGKIRHYGVSVEKVEEALKAIEYPNVKSV---QIIFNIFRQRP 184 + M+ LV+ GKI +GVS ++ +E P+ + Q+++N+ R+ Sbjct: 117 GAHPLAATIEGMETLVSQGKIGGWGVSNFDTDDIDDLLEEPHGEHYLCNQVLYNLSRRGI 176 Query: 185 IGLFLQEAQRRDIAVIARVPLASGLLTGKMNAQTTFAADDHRQFNRHGEAFDVGETFSGV 244 L +AQ+ IAV+A P+ G + +H Sbjct: 177 EYDLLPQAQQAHIAVMAYSPIEQG------------------RILKH------------- 205 Query: 245 PYEVALAAVEKIRALLPQGVPMAQFALRWILMESGISTVIPGARNVAQAQSNSAA 299 P ALA ++ AQ AL W+L + G+ IP A +VA Q+N A+ Sbjct: 206 PALTALAQAHRVTP--------AQIALAWVLRQPGV-VAIPKAADVAHVQANRAS 251 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 945,198 Number of extensions: 35263 Number of successful extensions: 131 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 123 Number of HSP's successfully gapped: 6 Length of query: 329 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 243 Effective length of database: 1,212,660 Effective search space: 294676380 Effective search space used: 294676380 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)