BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0047.p (522 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 968 0.0 gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 417 e-117 gi|300312486|ref|YP_003776578.1| major facilitator superfamily p... 216 4e-57 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 144 2e-35 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 126 5e-30 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 968 bits (2503), Expect = 0.0, Method: Compositional matrix adjust. Identities = 504/512 (98%), Positives = 507/512 (99%), Gaps = 2/512 (0%) Query: 1 MAT--TPPKPYTPPPPLTGANLVLGTLSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDE 58 MAT TP + YTPPPPLTGANLVLGTLSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDE Sbjct: 1 MATSPTPSQAYTPPPPLTGANLVLGTLSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDE 60 Query: 59 GTWVITSFAAANAISIPLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLPILLAARV 118 GTWVITSFAAANAISIPLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLPILLAARV Sbjct: 61 GTWVITSFAAANAISIPLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLPILLAARV 120 Query: 119 LQGLVAGPMVPLSQSLLLGAYPKSKSSFALALWGMTAVVAPVAGPALGGWITDSYTWPWI 178 LQGLVAGPMVPLSQSLLLGAYPKSKSSFALALWGMTAVVAPVAGPALGGWITDSYTWPWI Sbjct: 121 LQGLVAGPMVPLSQSLLLGAYPKSKSSFALALWGMTAVVAPVAGPALGGWITDSYTWPWI 180 Query: 179 FYINIPVGLIAAGVTLAIYRSRETPIHKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWFS 238 FYINIPVGLIAAGVTLAIYRSRETP HKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWF+ Sbjct: 181 FYINIPVGLIAAGVTLAIYRSRETPTHKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWFA 240 Query: 239 SPTIVTLGIIALIAFAYFVVWELNNDHPVVDLRLFGRRNFAGGTIAISIGYGVFFGNLVI 298 SPTIVTLGIIALIAFAYFVVWELNNDHPVVDLRLFGRRNFAGGTIAISIGYGVFFGNLVI Sbjct: 241 SPTIVTLGIIALIAFAYFVVWELNNDHPVVDLRLFGRRNFAGGTIAISIGYGVFFGNLVI 300 Query: 299 LPQWLQQYLGYRSIDSGLSTAPIGIFAVILMPLIGRFLPLVDARKVATASFIGFAIVFWL 358 LPQWLQQYLGYRSIDSGLSTAPIGIFAVILMPLIGRFLPLVDARKVATASFIGFAIVFWL Sbjct: 301 LPQWLQQYLGYRSIDSGLSTAPIGIFAVILMPLIGRFLPLVDARKVATASFIGFAIVFWL 360 Query: 359 RSRYNLQVDQMTVILPTLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGA 418 RSRYNLQVDQMTVILPTLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGA Sbjct: 361 RSRYNLQVDQMTVILPTLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGA 420 Query: 419 VGTSISTTAWNNRTIMHHAQLTEHATPYSPWLQETVNNLSSTLGLNAEQTAGMLERTITF 478 VGTSISTTAWNNR+IMHHAQLTEHATPYSPWLQETVNNLSSTLGLNAEQTAGMLERTITF Sbjct: 421 VGTSISTTAWNNRSIMHHAQLTEHATPYSPWLQETVNNLSSTLGLNAEQTAGMLERTITF 480 Query: 479 QAAMLGINDIFYVSAVIFIVIIPLIWIIKPSK 510 QAAMLGINDIFYVSAVIFIVIIPLIWIIKPSK Sbjct: 481 QAAMLGINDIFYVSAVIFIVIIPLIWIIKPSK 512 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 417 bits (1072), Expect = e-117, Method: Compositional matrix adjust. Identities = 221/509 (43%), Positives = 321/509 (63%), Gaps = 10/509 (1%) Query: 15 LTGANLVLGTLSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISI 74 L+G LV + ++ A F+ VLD++IANV++ TI+G LG S +GT+VITS+A A AI++ Sbjct: 26 LSGGQLVGAGILLAAANFIAVLDTTIANVSVSTIAGALGASTSQGTYVITSYAVAEAITV 85 Query: 75 PLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSL 134 PLTGWL R G VR+F+ S+++F L S LCG+A +L +L+A RVLQGL GP++PLSQ+L Sbjct: 86 PLTGWLANRFGTVRVFILSMVMFGLFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTL 145 Query: 135 LLGAYPKSKSSFALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTL 194 LL +PK K+ A+ LW MT +VAP+AGP LGG + D Y+WP+IF+IN+PV LI + Sbjct: 146 LLRIFPKEKAPAAVGLWAMTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYIGW 205 Query: 195 AIYRSRETPIHKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWFSSPTIVTLGIIALIAFA 254 + + ET + KLPID++GL L+V WVAA Q+MLD+GK+ DWF+S IV L + A++ F Sbjct: 206 RMLKRYETKMSKLPIDRVGLVLMVVWVAALQLMLDEGKEKDWFASTEIVLLAVTAVVFFI 265 Query: 255 YFVVWELNNDHPVVDLRLFGRRNFAGGTIAISIGYGVFFGNLVILPQWLQQYLGYRSIDS 314 F++WEL +P+VDLR+F R F I + +G FFG++V+ P WLQ Y+GY + S Sbjct: 266 AFLIWELTERNPIVDLRVFRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTATWS 325 Query: 315 GLSTAPIGIFAVILMPLIGRFLPLVDARKVATASFIGFAIVFWLRSRYNLQVDQMTVILP 374 G +A GI AV+ P++ + +DARK+ + +LR+ + + P Sbjct: 326 GCISALTGILAVMTAPMVAKMSTTIDARKLVCIGVTWLGFITFLRAFNTTDMSFFQIGWP 385 Query: 375 TLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAVGTSISTTAWNNRTIM 434 LQGI +FFVPLT L LS + E +AAGL SF R GA+ TSI T+W N Sbjct: 386 LFLQGIGLPLFFVPLTGLALSSVNEEETASAAGLMSFCRTMAGAIATSIVNTSWENDATY 445 Query: 435 HHAQLTEHATPYSPWLQETVNNLSSTLGLNAEQTAGMLERTITFQAAMLGINDIFYVSAV 494 HA+L+ + ++ + ++ G++AEQ L +T+ QA ML N IF ++AV Sbjct: 446 FHAELSGLVDRGG----DAIHTMMAS-GMSAEQATASLAQTVQNQAVMLSTNHIFMMAAV 500 Query: 495 IFIVIIPLIWII-KPSKGGAGAAEASAAH 522 F + +W+ KP++ A+ SAAH Sbjct: 501 SFSLAALAVWLAPKPTR----VADTSAAH 525 >gi|300312486|ref|YP_003776578.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 514 Score = 216 bits (549), Expect = 4e-57, Method: Compositional matrix adjust. Identities = 145/485 (29%), Positives = 248/485 (51%), Gaps = 8/485 (1%) Query: 28 SLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGAV 87 +L M LD SI N ++PTI G +G S EGTW+ T++ A + IP++ WL + LG Sbjct: 23 TLGALMATLDISIVNSSLPTIQGEIGASGTEGTWIATAYLVAEIVIIPMSAWLERLLGLR 82 Query: 88 RLFVTSVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSKSSFA 147 L +T+ LF L S LCGL+ TLP+++ RV QG G ++P + +++ P+S+ Sbjct: 83 VLLLTAASLFTLFSVLCGLSTTLPMMIVGRVGQGFTGGALIPTAMTIIATRLPRSQQPVG 142 Query: 148 LALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTL---AIYRSRETPI 204 A++G TA++ PV GP +GGW+T++ +W + F+IN+PVG AA +TL AI R Sbjct: 143 NAMFGATAILGPVLGPVMGGWLTENVSWHYAFFINLPVG--AALITLLLVAIPYERPKFE 200 Query: 205 HKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWFSSPTIVTLGIIALIAFAYFVVWELNND 264 D G+A L + ++L++G+ WFSSP I L +++ F + ++ Sbjct: 201 QLAEADWFGIAGLALGLGGLTVVLEEGEREQWFSSPDIRWLAATSVLGFILLAIGQVRAR 260 Query: 265 HPVVDLRLFGRRNFAGGTIAISIGYGV-FFGNLVILPQWLQQYLGYRSIDSGLSTAPIGI 323 HPV+ LRL R F G + +++ GV +G ++PQ+L GY S+ SG G+ Sbjct: 261 HPVIQLRLLRDRQF-GSVVMMAMIVGVALYGTAYVIPQFLSGIAGYNSLQSGWIVLLSGV 319 Query: 324 FAVILMPLIGRFLPLVDARKVATASFIGFAIVFWLRSRYNLQVDQMTVILPTLLQGIPTA 383 +++MP + LVD R F+ A ++ + + + + L++G+ T Sbjct: 320 PMILMMPFTPLLMRLVDIRIAVGTGFLLLAASAFIETDLSPLSYGGSFVASQLMRGVGTV 379 Query: 384 MFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAVGTSISTTAWNNRTIMHHAQLTEHA 443 + + L + +PP + A+GL S R G++ + + R +H +L E Sbjct: 380 LAMLFLNQAAIRSVPPSQAGDASGLYSAARNLGGSLALAAIAVIQDQRLWLHSRRLEESL 439 Query: 444 TPYSPWLQETVNNLSSTLGLNAEQTAGMLERTITFQAAMLGINDIFYVSAVIFIVIIPLI 503 S +Q ++ LG + ++ TI QA + D+F++ V + + PL+ Sbjct: 440 NANSDLVQSSMTAQGHLLG-GQDVALQLMGATIQSQALTMTYADLFWMLGVAILCVTPLV 498 Query: 504 WIIKP 508 ++P Sbjct: 499 LFLRP 503 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 144 bits (362), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 127/414 (30%), Positives = 196/414 (47%), Gaps = 25/414 (6%) Query: 27 VSLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGA 86 V+ FM LD++I N A+P ++ +LG V V+ ++ A P +GWL R G Sbjct: 58 VASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFGT 117 Query: 87 VRLFVTSVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSKSSF 146 R++ ++LLF L S LC +A +LP+LLAARV+QGL ++P+ + +L A P ++ Sbjct: 118 RRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYIA 177 Query: 147 ALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYRSRETPIHK 206 ALA + + PV GP LGGW+ +S +W WIF IN+PVG++ G L Y + + Sbjct: 178 ALASISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVL--YFLPDDSLAG 235 Query: 207 LP-IDKIGLALLVTWVAAFQIMLDKGKDLDWFSSPTIVTLGIIALIAFAYFVVWELNNDH 265 P D +G LL + AF + LD D S T ++ + + A W + H Sbjct: 236 TPRFDLLGCVLLSACMVAFSLALDVPFD----SHRTGWSIALFVVAALTVLGYWRHASVH 291 Query: 266 --PVVDLRLFGRRNFA---GGTIAISIG-YGVFFGNLVILPQWLQQYLGYRSIDSGLSTA 319 P+ L LFG NF+ G + IG V F ++ LQ LGY + +G+ Sbjct: 292 PRPLFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLPLL----LQVRLGYSPLHAGMVLL 347 Query: 320 PIGIFAVI----LMPLIGRFLPLVDARKVATASFIGFAIVFWLRSRYNLQVDQMTVILPT 375 P I I + PL+ RF D + + +G AI+ + + + V L Sbjct: 348 PAAIAGTIAKPWIAPLVKRF--GYDRFLLVNTAIVGAAIMSFALITPDWPLTAAIVQLAI 405 Query: 376 LLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAVGTSISTTAWN 429 G +M F + + L GL + L S V++ +G +I T N Sbjct: 406 F--GASNSMQFAAMNSVTLKGLDKRDAGSGNSLFSMVQMLAIGLGVTIGGTMVN 457 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 126 bits (316), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 109/414 (26%), Positives = 195/414 (47%), Gaps = 15/414 (3%) Query: 36 LDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGAVRLFVTSVL 95 +DS++ + ++P ++ ++ V +TS+ + A+ IP++GW+ R G++ +F +++ Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 96 LFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSKSSFALALWGMTA 155 +F+L S LCG++ TLP +AAR LQG+ MVP+ + ++L + K AL+ + A Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 156 VVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYRSRETPIHK----LPIDK 211 + PV GP LGG+IT + W WIF+INIP+ ++ G+ LA +R P K P+D Sbjct: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISIL--GLYLA---TRFIPDLKAEQVAPLDL 175 Query: 212 IGLALLVTWVAAFQIMLDKGKDLDWFSSPTIVTLGIIALIAFAYFVVWELNN-DHPVVDL 270 G L++ + +M+ S + ++A + F VW P++DL Sbjct: 176 KG--FLLSALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDL 233 Query: 271 RLFGRRNFAGGTIAISIGYGVFFGNL-VILPQWLQQYLGYRSIDSGLSTAPIGIFAVILM 329 L G S Y V G + +LP LQ GY SG+ T A+ + Sbjct: 234 ALLKIPTLRAGVFGGSF-YRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMK 292 Query: 330 PLIGRFLPLVDARKVATASFIGFAIVFWLRSRYNLQVDQMTVILPTLLQGIPTAMFFVPL 389 + RFL R+V + + W + + Q + +++ L+ G ++ F L Sbjct: 293 TIAARFLRRYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQFTSL 352 Query: 390 TVLLLSGLPPERIPAAAGLSSFVRVFCGAVGTSISTTAWN-NRTIMHHAQLTEH 442 + + + R+ A ++S + +G ++ A + I HA L Sbjct: 353 NAISYADVDKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHATLVAQ 406 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.139 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,496,731 Number of extensions: 65026 Number of successful extensions: 425 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 413 Number of HSP's successfully gapped: 17 Length of query: 522 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 432 Effective length of database: 1,193,720 Effective search space: 515687040 Effective search space used: 515687040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)