BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0046.p (408 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312016|ref|YP_003776108.1| multidrug resistance A (translo... 693 0.0 gi|300312565|ref|YP_003776657.1| multidrug resistance transmembr... 280 1e-76 gi|300311421|ref|YP_003775513.1| multidrug resistance efflux pum... 77 3e-15 gi|300312487|ref|YP_003776579.1| multidrug resistance transport ... 57 2e-09 >gi|300312016|ref|YP_003776108.1| multidrug resistance A (translocase) transmembrane secretion protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 693 bits (1788), Expect = 0.0, Method: Compositional matrix adjust. Identities = 397/408 (97%), Positives = 403/408 (98%), Gaps = 2/408 (0%) Query: 1 MSDSTTQQNPQANSNGNGNGKRKRQLILLTLVLLVIAIACFLYWFLHARFFEETDDAYVG 60 MSDSTTQQ PQAN+NGNG KRKRQLILLTLVLLVIA+ACFLYWFLHARFFEETDDAYVG Sbjct: 1 MSDSTTQQQPQANNNGNG--KRKRQLILLTLVLLVIAVACFLYWFLHARFFEETDDAYVG 58 Query: 61 GNVVQISAQVGGTVVAVKADDTQVVKAGQPLVALDAADTKLALEQAQAALAQAVRQTRQL 120 GNVVQISAQVGGTVVAVKADDTQVVKAGQ LVALDAADT+LAL+QAQAALAQAVRQTRQL Sbjct: 59 GNVVQISAQVGGTVVAVKADDTQVVKAGQQLVALDAADTRLALDQAQAALAQAVRQTRQL 118 Query: 121 FLNNDTLAANVAAADSNLARAREDLQRRQAGLSSGAVSQEDVSHARDAVKSAVAALDQAR 180 FLNNDTLAANVAAADSNLARAREDLQRRQAGLSSGAVSQEDVSHARDAVKSAVAALDQAR Sbjct: 119 FLNNDTLAANVAAADSNLARAREDLQRRQAGLSSGAVSQEDVSHARDAVKSAVAALDQAR 178 Query: 181 AAAAANRALTDHTSVTEHPNVLQAATAVRNAYLNYARVNIVAPVSGFVSKRSVQVGQRIA 240 AAAAANRALTDHTSVTEHPNVLQAATAVRNAYLNYARVNIVAPVSGFVSKRSVQVGQRIA Sbjct: 179 AAAAANRALTDHTSVTEHPNVLQAATAVRNAYLNYARVNIVAPVSGFVSKRSVQVGQRIA 238 Query: 241 AGNPLMAIVPLEQIWIDANFKESQLQHIRLGQPVEVIADVYGSSVKYKGTVIGFSAGTGG 300 AGNPLMAIVPLEQIWIDANFKESQLQHIR+GQPVEVIADVYGSSVKYKGTVIGFSAGTGG Sbjct: 239 AGNPLMAIVPLEQIWIDANFKESQLQHIRIGQPVEVIADVYGSSVKYKGTVIGFSAGTGG 298 Query: 301 AFSLLPAQNATGNWIKVVQRVPVRIALDPEQVRAHPLRIGLSTTATVDIHGDGRALEAVP 360 AFSLLPAQNATGNWIKVVQRVPVRIALDPEQVRAHPLRIGLSTTATVDIHGDGRALEAVP Sbjct: 299 AFSLLPAQNATGNWIKVVQRVPVRIALDPEQVRAHPLRIGLSTTATVDIHGDGRALEAVP 358 Query: 361 TNYQTNVYDDLGKQADAIVERIISDNASGLPQARKSKAAAAPVAVPHT 408 TNYQTNVYDDLGKQADAIV+RIISDNASGLPQA KSKAAAAPVAVPHT Sbjct: 359 TNYQTNVYDDLGKQADAIVDRIISDNASGLPQAHKSKAAAAPVAVPHT 406 >gi|300312565|ref|YP_003776657.1| multidrug resistance transmembrane efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 411 Score = 280 bits (716), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 155/359 (43%), Positives = 218/359 (60%), Gaps = 28/359 (7%) Query: 21 KRKRQLILLTLVLLVIAIACFLYWFLHARFFEETDDAYVGGNVVQISAQVGGTVVAVKAD 80 KRK+ + V+ + AI YW+L + ETD+AY + ++ + G V AV Sbjct: 31 KRKKLFSIFGGVVAIAAIGYGAYWYLIGSRYVETDNAYTATEIATVTPAINGIVAAVDVV 90 Query: 81 DTQVVKAGQPLVALDAADTKLALEQAQAALAQAVRQTRQLFLNNDTLAANVAA------- 133 DTQ VK G LV +D AD +LA++QA A L + R+ + F N+ LAA V A Sbjct: 91 DTQAVKKGDVLVRIDDADARLAVDQAAADLDRTERKVKGFFANDAGLAAQVLAREAEQKR 150 Query: 134 -------ADSNLARAREDLQRRQAGLSSGAVSQEDVSHARDAVKSAVAALDQARAAAAAN 186 A ++L RA DLQRR+A SG+VS E++S+AR A+ +A A L A AA + Sbjct: 151 ASAQLLSAQADLKRAEIDLQRREALAKSGSVSGEELSNARTALLTAQANLKAAEAAEVQS 210 Query: 187 RA--------------LTDHTSVTEHPNVLQAATAVRNAYLNYARVNIVAPVSGFVSKRS 232 RA LT +T+V ++P V+ A + A L+ R + APV G +++R Sbjct: 211 RANIKATQGAQKASTVLTANTTVDDNPEVVLARAKLEQAKLDLERTVLRAPVDGVIARRQ 270 Query: 233 VQVGQRIAAGNPLMAIVPLEQIWIDANFKESQLQHIRLGQPVEVIADVYGSSVKYKGTVI 292 VQVGQR+ +G L+++VPL+Q+ +DANFKE QL +R+GQPV + AD+YG SV+Y G V Sbjct: 271 VQVGQRVQSGATLLSVVPLQQMHVDANFKEGQLTKVRIGQPVTMKADLYGGSVEYHGVVT 330 Query: 293 GFSAGTGGAFSLLPAQNATGNWIKVVQRVPVRIALDPEQVRAHPLRIGLSTTATVDIHG 351 G S GTG AF+++PAQNATGNWIKVVQR+PVRI+LDP+++ PL +GLS +D G Sbjct: 331 GLSGGTGSAFAVIPAQNATGNWIKVVQRLPVRISLDPKELAQRPLSVGLSMVVEIDTRG 389 >gi|300311421|ref|YP_003775513.1| multidrug resistance efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 324 Score = 77.0 bits (188), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 98/341 (28%), Positives = 156/341 (45%), Gaps = 41/341 (12%) Query: 24 RQLILLTLVLLVIAIACFLY-WFLHARFFEETDDAYVGGNVVQISAQVGGTVVAVKADDT 82 R LI + LL I +A L+ ++H+ + T D V VV I+ V G VVA+ D Sbjct: 8 RVLITTAIFLLAILLAWSLWQHYMHSPW---TRDGRVRAEVVNIAPDVSGQVVALPVRDN 64 Query: 83 QVVKAGQPLVALDAADTKLALEQAQAALAQAVRQTRQLFLNNDTLAANVAAADSNLARAR 142 Q+VK G L+ +D A LAL+QA+AA V A + L R Sbjct: 65 QLVKKGDLLMEIDPARYTLALQQAEAA---------------------VTARKAELDVRR 103 Query: 143 EDLQRRQAGLSSGAVSQEDVSHARDAVKSAVAALDQ-ARAAAAANRALTDHTSVTEHPNV 201 +++ R+A L+ + V S A D ++ A+ + + Sbjct: 104 AEVEARRADLNMRRAQARRRADVDALVVSREAREDAGSQVTASQAQLEAAQAQLQGAQAQ 163 Query: 202 LQAATAVRN-AYLNYARVNIVAPVSGFVSKRSVQVGQRIAAGNPLMAIVPLEQIWIDANF 260 QAA + R+ A LN R + +PV G+++ +V+ G AAG +A++ W+ A F Sbjct: 164 YQAALSQRDTARLNLERTKVFSPVDGYITNLNVRRGDYAAAGAAKIAVIDSHSFWVYAYF 223 Query: 261 KESQLQHIRLGQPVEVIADVYGSSVKYKGTVIGFSAGTGG-----AFSLLPAQNATGNWI 315 +E++L + +G I + G+S+ +G V + G + L N T NW+ Sbjct: 224 EETKLPLLSIGDRAR-IQLINGASM--EGHVDSIARGIYDRDNPQSRELTADVNPTFNWV 280 Query: 316 KVVQRVPVRIALD--PEQVRAHPLRIGLSTTATVDIHGDGR 354 ++ QR+PVRI LD PE V L GL+ T ++ G G+ Sbjct: 281 RLAQRIPVRIQLDKVPENVL---LASGLTCTVVLE-PGSGK 317 >gi|300312487|ref|YP_003776579.1| multidrug resistance transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 397 Score = 57.4 bits (137), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 41/113 (36%), Positives = 60/113 (53%), Gaps = 15/113 (13%) Query: 1 MSDSTTQQNP----QANS----NGNGNGKRKRQLILLTLVL-------LVIAIACFLYWF 45 M+D+T Q P QA++ + G+ +KR +L LVI A LY+ Sbjct: 1 MTDATPHQTPAEPDQADAPRSDDKQGSAPKKRMNPRARFILRALLVVVLVIVAAWVLYYQ 60 Query: 46 LHARFFEETDDAYVGGNVVQISAQVGGTVVAVKADDTQVVKAGQPLVALDAAD 98 ++ E T+DA+V + V +S++V G V V D Q+VKAGQPLV +DA D Sbjct: 61 FRGKYLESTNDAFVQADSVTVSSKVSGYVDQVLVADNQIVKAGQPLVRIDARD 113 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.129 0.357 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,003,664 Number of extensions: 37406 Number of successful extensions: 202 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 191 Number of HSP's successfully gapped: 6 Length of query: 408 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 321 Effective length of database: 1,207,925 Effective search space: 387743925 Effective search space used: 387743925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)