BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0045.p (318 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312015|ref|YP_003776107.1| NodT family protein [Herbaspiri... 548 e-157 gi|300312564|ref|YP_003776656.1| outer membrane multidrug resist... 184 7e-48 gi|300311423|ref|YP_003775515.1| NodT family protein [Herbaspiri... 177 1e-45 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 130 1e-31 gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 121 1e-28 >gi|300312015|ref|YP_003776107.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 495 Score = 548 bits (1411), Expect = e-157, Method: Compositional matrix adjust. Identities = 274/310 (88%), Positives = 297/310 (95%) Query: 1 VGYELDLWNKNQSALEQAISSEKAAQASEQEARLALTASIVSVYSQLAAQYAVQDILQRT 60 VGYELDLWNKNQ+AL QAISSEKAAQASEQEARLALTASIV+VYSQLAAQYA+ DILQ T Sbjct: 153 VGYELDLWNKNQAALAQAISSEKAAQASEQEARLALTASIVTVYSQLAAQYALHDILQST 212 Query: 61 VQQRSNLETITADRVKTGLDSQIERNQSRTSSADARAQLEQNAGQIVLLRQQLGALSGKG 120 V QR++LE ITA+R++TGLDSQIER+QSRTSSADARAQL Q+ GQIVLLRQQLGAL+GKG Sbjct: 213 VDQRTSLEKITAERLRTGLDSQIERDQSRTSSADARAQLAQSEGQIVLLRQQLGALAGKG 272 Query: 121 PDRGLQLAPPAMQNLATPGLPADLPLNLLGRRPDIVAARWQVEAASRGVDVAKARFYPDI 180 PD GLQLAPPA+Q LATPGLPA+LPLNL+GRRPDIVAARWQVEAASRGVDVAKARFYPDI Sbjct: 273 PDYGLQLAPPALQGLATPGLPAELPLNLMGRRPDIVAARWQVEAASRGVDVAKARFYPDI 332 Query: 181 NLSAMIGFDSLVDANPFTAASKSIAFGPAITLPIFEGGALRAGLKGEYANYELAVATYNK 240 NLSAMIGFD+L+D+NPFTAASKSIAFGPAITLPIFEGGALRAGLKGEYA+YELAVATYNK Sbjct: 333 NLSAMIGFDTLLDSNPFTAASKSIAFGPAITLPIFEGGALRAGLKGEYASYELAVATYNK 392 Query: 241 TLNDAYADVARQIAAIHSIERQLPIRREALQAAERAYDLARERYRLGLVSQLTLLSSETG 300 TLNDAYADVARQIAAIH+ ERQLPIR EALQAAERAY LARERYRLGLVSQLTLLS++TG Sbjct: 393 TLNDAYADVARQIAAIHATERQLPIRSEALQAAERAYALARERYRLGLVSQLTLLSAQTG 452 Query: 301 VLAQRQSLTA 310 VLAQRQ++ A Sbjct: 453 VLAQRQAMVA 462 >gi|300312564|ref|YP_003776656.1| outer membrane multidrug resistance lipoprotein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 184 bits (467), Expect = 7e-48, Method: Compositional matrix adjust. Identities = 122/308 (39%), Positives = 178/308 (57%), Gaps = 2/308 (0%) Query: 3 YELDLWNKNQSALEQAISSEKAAQASEQEARLALTASIVSVYSQLAAQYAVQDILQRTVQ 62 YE+D W KN++AL A S +A++A +AR+ L SI S Y++LA YA +D + ++ Sbjct: 165 YEIDFWGKNRAALAAATSELEASRADAAQARITLATSIASAYAELAQLYAQRDTNEAALK 224 Query: 63 QRSNLETITADRVKTGLDSQIERNQSRTSSADARAQLEQNAGQIVLLRQQLGALSGKGPD 122 R + R GL+++ Q A A+A L+ I L R +L AL G GPD Sbjct: 225 VRVESLDLFNQRFTNGLETRGSVKQMEARRAIAQADLKATDESIGLQRNKLAALLGAGPD 284 Query: 123 RGLQLAPPAMQNLATP-GLPADLPLNLLGRRPDIVAARWQVEAASRGVDVAKARFYPDIN 181 RGLQL P + +L+ P LPA LP+ LLGRRPDIVAAR + EAA + + VA+A FYP++N Sbjct: 285 RGLQLTRPQI-DLSRPFALPAQLPVELLGRRPDIVAARLRAEAAGKQIKVARAAFYPNVN 343 Query: 182 LSAMIGFDSLVDANPFTAASKSIAFGPAITLPIFEGGALRAGLKGEYANYELAVATYNKT 241 L+A GF SL A S + GPAI+LPIF GG LR + A+Y+ AVA Y++ Sbjct: 344 LTAYFGFQSLGIDMLTRAGSDIGSIGPAISLPIFNGGRLRGQFRSASASYDEAVANYDQA 403 Query: 242 LNDAYADVARQIAAIHSIERQLPIRREALQAAERAYDLARERYRLGLVSQLTLLSSETGV 301 + A DVA + ++ +L + A AAE AY + RY GL + L +L+++ + Sbjct: 404 VTQALQDVADVGVSEKALAGRLADVQAAADAAEEAYRIVSNRYNGGLATYLDVLNAQDTL 463 Query: 302 LAQRQSLT 309 ++ + L+ Sbjct: 464 ISNLRQLS 471 >gi|300311423|ref|YP_003775515.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 487 Score = 177 bits (448), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 114/299 (38%), Positives = 173/299 (57%), Gaps = 2/299 (0%) Query: 3 YELDLWNKNQSALEQAISSEKAAQASEQEARLALTASIVSVYSQLAAQYAVQDILQRTVQ 62 Y +DLW++N++A +A A A + A+L L ++V Y QLA QY + D + ++ Sbjct: 147 YNVDLWDRNKNATLRAQDEMHAVAADARAAQLDLEGNVVRTYVQLALQYGLLDNTEALLK 206 Query: 63 QRSNLETITADRVKTGLDSQIERNQSRTSSADARAQLEQNAGQIVLLRQQLGALSGKGPD 122 + + + R K GL +Q+E Q+ + + R Q+E I L+R QL AL G GP Sbjct: 207 EEQKILDLAHRRFKGGLGTQLEITQAESPLPETRRQIEALHENIALIRNQLAALLGAGPG 266 Query: 123 RGLQLAPPAMQNLATPGLPADLPLNLLGRRPDIVAARWQVEAASRGVDVAKARFYPDINL 182 + P MQ + GLP+ LP +L+GRRPDI AARW+VEAA++G+ VAKA FYP+INL Sbjct: 267 AADGVTRPGMQLQTSLGLPSALPADLVGRRPDISAARWRVEAAAKGISVAKAAFYPNINL 326 Query: 183 SAMIGFDSLVDA--NPFTAASKSIAFGPAITLPIFEGGALRAGLKGEYANYELAVATYNK 240 A IG + + +A + +GPAI+LPIFEGG LR L A Y++ V YN Sbjct: 327 LAGIGPAAAGGGLFSFLSAPNLQTTYGPAISLPIFEGGRLRGQLGAASAGYDIEVEHYNA 386 Query: 241 TLNDAYADVARQIAAIHSIERQLPIRREALQAAERAYDLARERYRLGLVSQLTLLSSET 299 L+ A ++ Q+ + S+ RQ ++++QAA++ D+A Y+ GL L +L ++T Sbjct: 387 LLSQALKGISDQVVTLQSVARQQEQAQQSVQAAQKNVDIATRAYQRGLTDYLNVLHAQT 445 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 130 bits (327), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 103/318 (32%), Positives = 166/318 (52%), Gaps = 20/318 (6%) Query: 3 YELDLWNKNQSALEQAISSEKAAQASEQEARLALTASIVSVYSQLAAQYAVQDILQRTVQ 62 YELDL+ + +S EQA+ S + + + +++L A + + + LA+ + Q T++ Sbjct: 150 YELDLFGRVRSLKEQALQSFLSTAEARRSTQISLIAEVATAWLTLASDQDRLRLAQDTLE 209 Query: 63 QRSNLETITADRVKTGLDSQIERNQSRTSSADARAQLEQNAGQIVLLRQQLGALSGKG-- 120 +S+ +T + G S + Q++TS AR +E Q+ R L L GK Sbjct: 210 SQSSSYALTQRSFELGSSSALTLRQAQTSVESARVDVESYTAQVDQDRNALVLLVGKDLP 269 Query: 121 --------PDRGLQLAPPAMQNLAT--PGLPADLPLNLLGRRPDIVAARWQVEAASRGVD 170 PD L A P LAT PGLP+DL L RR DIV A + AA+ + Sbjct: 270 TALLPQGLPDTRLAAASP----LATIPPGLPSDL----LQRRADIVQAERDLRAANANIG 321 Query: 171 VAKARFYPDINLSAMIGFDSLVDANPFTAASKSIAFGPAITLPIFEGGALRAGLKGEYAN 230 A+A FYP I L+ G S A F S S +F P I+LPIF+GGA +A L + Sbjct: 322 AARAAFYPSITLTVNAGTASARLAGLFKGGSGSWSFVPQISLPIFDGGANQANLDIATVS 381 Query: 231 YELAVATYNKTLNDAYADVARQIAAIHSIERQLPIRREALQAAERAYDLARERYRLGLVS 290 +++VA Y K + A+ +V+ +A +++ RQL + +QA++ A+ L++ RY G+ S Sbjct: 382 RDISVAQYEKAIQTAFREVSDALAQRNTLGRQLQAQEALVQASDEAFRLSQARYHSGVDS 441 Query: 291 QLTLLSSETGVLAQRQSL 308 LT+L S+ + + +++L Sbjct: 442 YLTVLDSQRTLYSAQKNL 459 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 121 bits (303), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 91/309 (29%), Positives = 158/309 (51%), Gaps = 6/309 (1%) Query: 3 YELDLWNKNQSALEQAISSEKAAQASEQEARLALTASIVSVYSQLAAQYAVQDILQRTVQ 62 YELD + + ++ + A+ S + + + ++L A + + + LA+ + + Q T Sbjct: 157 YELDFFGRLKNLSDAALESYQYQVETRRSTHISLVAEVATAWLTLASDRDLLKLAQDTYA 216 Query: 63 QRSNLETITADRVKTGLDSQIERNQSRTSSADARAQLEQNAGQIVLLRQQLGALSG-KGP 121 +S ++ G S ++ + ARA + + A Q+ Q L AL G Sbjct: 217 SQSRTFALSQRSHDIGTVSGVDLASQEATVESARADVARYASQV---EQDLNALRLLVGA 273 Query: 122 DRGLQLAPPAMQNLAT--PGLPADLPLNLLGRRPDIVAARWQVEAASRGVDVAKARFYPD 179 D L P + + A+ P PA LP +L RRPD++AA +++A+ + A+A F+P Sbjct: 274 DIDAALLPAGLPDTASVLPATPAGLPSQVLLRRPDVLAAEHTLKSANADIGAARAAFFPS 333 Query: 180 INLSAMIGFDSLVDANPFTAASKSIAFGPAITLPIFEGGALRAGLKGEYANYELAVATYN 239 I+L+A G S F A S S F P+I LPIF G+LRA L ++ VA Y Sbjct: 334 ISLTANGGTASRTLGGLFKAGSGSWTFSPSINLPIFNAGSLRASLDSAKITRDIDVANYE 393 Query: 240 KTLNDAYADVARQIAAIHSIERQLPIRREALQAAERAYDLARERYRLGLVSQLTLLSSET 299 K + A+ +VA +A +++++L + +A A+E+A+ L+ RYR G+ S LT L ++ Sbjct: 394 KAIQTAFREVADALAVRATLDQRLDAQGKATAASEKAFRLSDARYRNGVDSYLTTLVAQR 453 Query: 300 GVLAQRQSL 308 + + +Q+L Sbjct: 454 TLYSAQQTL 462 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.128 0.347 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 871,817 Number of extensions: 35803 Number of successful extensions: 180 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 162 Number of HSP's successfully gapped: 18 Length of query: 318 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 233 Effective length of database: 1,217,395 Effective search space: 283653035 Effective search space used: 283653035 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)