BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0041.p (588 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane... 932 0.0 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 186 3e-48 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 186 3e-48 gi|300311453|ref|YP_003775545.1| signal transduction containing ... 183 3e-47 gi|300311036|ref|YP_003775128.1| signal transduction protein [He... 181 1e-46 >gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 797 Score = 932 bits (2408), Expect = 0.0, Method: Compositional matrix adjust. Identities = 483/615 (78%), Positives = 525/615 (85%), Gaps = 30/615 (4%) Query: 1 VRLYRLESKIVALFIILIVVVQLAGFVAIRTAIDRNARSAISEELIIGERVFTRLLDQNA 60 VRLYRLESKIVALFI L+VVVQLAGFVAIRTAIDRNAR++ISEEL +GERVF R LDQNA Sbjct: 2 VRLYRLESKIVALFITLLVVVQLAGFVAIRTAIDRNARASISEELAVGERVFARQLDQNA 61 Query: 61 QKLTQGARLLASDFGFRQAIGTDDRDTIASVLANHGARIGATMGMLVGTDGLIHASTLEY 120 QKLTQGARLLASDFGFRQAIGTDDR+TIASVL+NHGARIGA+M ML+G DG I AST+EY Sbjct: 62 QKLTQGARLLASDFGFRQAIGTDDRETIASVLSNHGARIGASMAMLIGNDGQIRASTVEY 121 Query: 121 PDTDLQRSSLELVRKAGNGNGAAESAVVGDNLFQIVAVPIKAPVTIAWVVMGFPVDRDLV 180 PDTDLQRSSLELVRKAGNGNGA+E+AVVGD LFQIVAVPIKAPVTIAWVVMGFP+D LV Sbjct: 122 PDTDLQRSSLELVRKAGNGNGASENAVVGDRLFQIVAVPIKAPVTIAWVVMGFPIDHQLV 181 Query: 181 NDMRALSQLQVSILVRQRGSAGWSADVSTLPKAEAQMMAKEASSPQMGQAGATDLQIDGN 240 +DMR LS LQVSILV QRG GW+ADVSTL A M+ KE P M G DLQIDGN Sbjct: 182 SDMRELSSLQVSILVSQRGGVGWNADVSTLTAPAASMLLKEVGQPAMRAGGLIDLQIDGN 241 Query: 241 QYRARVLPLARA------------------------------LXVLGVLGAAVASAVMAR 270 QYRAR+LPLA+A + VLGVLGAAVASAV+AR Sbjct: 242 QYRARLLPLAQASNGQQAVAVLQRSVSEAVEPYQRLQLILLSITVLGVLGAAVASAVLAR 301 Query: 271 RITSPLRDLATVAKRLGEGDYSEGLYIRRQDEIGRLGAAFETMRSEIASRELKISRLAYQ 330 RITSPLRDLA VAKRLGEGDYSEGL+IRRQDEIGRLG AFETMR EIASRELKISRLAYQ Sbjct: 302 RITSPLRDLADVAKRLGEGDYSEGLFIRRQDEIGRLGNAFETMRLEIASRELKISRLAYQ 361 Query: 331 DQLTGLPNRVQFGDLLREAIGQAQALPGGSCHILMMDLDRFQQVNDVLGHSFGDDLLRQV 390 DQLTGLPNRVQF DLLR+AI +A PG +C+ILMMDLDRFQQVNDVLGHSFGDDLLRQV Sbjct: 362 DQLTGLPNRVQFADLLRDAIAEAARTPGAACYILMMDLDRFQQVNDVLGHSFGDDLLRQV 421 Query: 391 AARLDAQLLYPGAKVARLGGDEFAILLPGVDLAGALDQAAHILRALEQPLSLQEQTVDLG 450 A RL AQLL+PGAK++RLGGDEFAILLPGVD GA++QA ILRALEQPLSL +QTVDLG Sbjct: 422 ALRLSAQLLHPGAKLSRLGGDEFAILLPGVDQEGAVEQALQILRALEQPLSLHQQTVDLG 481 Query: 451 AGIGVAGYPAHGDSSEVLLSRAEVAMYTAKRKGNGVTIYDPAIDQSSQESLSLLSELRRA 510 AGIG++GYPAHG SS+VLLS AEVAMY AKRKG+G+ IYDPAIDQSSQESLSLLSELRRA Sbjct: 482 AGIGISGYPAHGQSSDVLLSHAEVAMYIAKRKGSGLVIYDPAIDQSSQESLSLLSELRRA 541 Query: 511 LERDEFMLYLQPKLDLATGAVVGGEALVRWVHPEKGFIGPDNFIPFAETTGFIRMLTIWM 570 LERDEFMLYLQPKL+L +G VVG EALVRW+HPEKGFIGPDNFIPFAETTGFIRMLT+WM Sbjct: 542 LERDEFMLYLQPKLELCSGQVVGAEALVRWIHPEKGFIGPDNFIPFAETTGFIRMLTLWM 601 Query: 571 MEKVAGLSARFKGGG 585 ME+VA LS + + G Sbjct: 602 MEQVARLSEQLQQQG 616 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 186 bits (473), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 113/290 (38%), Positives = 158/290 (54%), Gaps = 7/290 (2%) Query: 301 DEIGRLGAAFETMR--SEIASRELKISRLAYQDQLTGLPNRVQFGDLLREAIGQAQALPG 358 DE G+L T+ +E + E ++ A D LTGLPNR + L+ A+ A+ G Sbjct: 237 DEAGKLSCYEGTVEAITERKAHEAEMRHQATHDALTGLPNRNMLHEHLQRALRVARQ-KG 295 Query: 359 GSCHILMMDLDRFQQVNDVLGHSFGDDLLRQVAARLDAQLLYPGAKVARLGGDEFAILLP 418 + +DLD+F+ +ND LGH GD+LL+ VA RL A L VAR GGDEF ++L Sbjct: 296 ALTAVAFVDLDQFKFINDSLGHQVGDELLKTVAQRLQA-CLREADMVARQGGDEFVLVLQ 354 Query: 419 GVDL--AGALDQAAHILRALEQPLSLQEQTVDLGAGIGVAGYPAHGDSSEVLLSRAEVAM 476 AG + IL A+ +P ++ + A IGV+ YPA G E LL A+ AM Sbjct: 355 NQTEGEAGIAEVMQRILAAVARPWQAGDREFQVTASIGVSRYPADGKDVESLLKHADSAM 414 Query: 477 YTAKRKG-NGVTIYDPAIDQSSQESLSLLSELRRALERDEFMLYLQPKLDLATGAVVGGE 535 Y AK +G N + P +D L +L LRRAL++DEF LY QPKL L G ++G E Sbjct: 415 YRAKEQGRNNFQFFAPWMDSQVSNRLEMLINLRRALDQDEFKLYYQPKLSLQDGRLIGAE 474 Query: 536 ALVRWVHPEKGFIGPDNFIPFAETTGFIRMLTIWMMEKVAGLSARFKGGG 585 AL+RW P++G + PD FIPFAE G I + W++ + ++ G Sbjct: 475 ALIRWESPQQGMVPPDRFIPFAEEAGLIVPIGEWVLRTACRQNQAWQQAG 524 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 186 bits (473), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 114/280 (40%), Positives = 157/280 (56%), Gaps = 16/280 (5%) Query: 312 TMRSEIASRELKISRLAYQDQLTGLPNRVQFGDLLREAIG----QAQALPGGSCHILMMD 367 +M+ A E + SR D LTGL NR L +AIG Q QAL ++ +D Sbjct: 507 SMKRYEAELEFQASR----DTLTGLINRHMLRHHLEQAIGVAERQKQAL-----WVVFVD 557 Query: 368 LDRFQQVNDVLGHSFGDDLLRQVAARLDAQLLYPGAKVARLGGDEFAILLPGVD-LAGAL 426 LDRF+ VND LGH GD LLR VA RL A + VARLGGDEF ++LP D L Sbjct: 558 LDRFKFVNDTLGHKAGDTLLRTVAGRLRAAV-READTVARLGGDEFIMILPEQDELTLDT 616 Query: 427 DQAAHILRALEQPLSLQEQTVDLGAGIGVAGYPAHGDSSEVLLSRAEVAMYTAKRKG-NG 485 I+ A+ P++L T+ + +GVA YP G E L+ A++AMY K+ G N Sbjct: 617 RSLQRIMDAVAAPVTLGGHTLFMTCSVGVACYPNDGTDGETLIKHADIAMYRGKQTGRNN 676 Query: 486 VTIYDPAIDQSSQESLSLLSELRRALERDEFMLYLQPKLDLATGAVVGGEALVRWVHPEK 545 Y PA+++ + E L + +LR AL+ EF+L+ QPK+DLA+G V+G EAL+RW HP+ Sbjct: 677 FQFYTPAMNEQALERLRIEGDLRNALDHGEFLLHYQPKVDLASGRVIGSEALIRWQHPQL 736 Query: 546 GFIGPDNFIPFAETTGFIRMLTIWMMEKVAGLSARFKGGG 585 G + P FI AE TG I + W++ +++ GG Sbjct: 737 GMVSPARFIGLAEETGLILPIGAWVLRAACTQCRQWQLGG 776 >gi|300311453|ref|YP_003775545.1| signal transduction containing PAS/GGDEF/EAL domains protein [Herbaspirillum seropedicae SmR1] Length = 683 Score = 183 bits (464), Expect = 3e-47, Method: Compositional matrix adjust. Identities = 107/275 (38%), Positives = 156/275 (56%), Gaps = 6/275 (2%) Query: 315 SEIASRELKISRLAYQDQLTGLPNRVQFGDLLREAIGQAQALPGGSCHILMMDLDRFQQV 374 +E + E I LAY D LTGLPNR + +A+ +A+ C ++ +DL+RF+ + Sbjct: 231 TERKNAEKLIHHLAYYDALTGLPNRTLLTRTVDQALEEARHENLHGC-VMFIDLNRFKPI 289 Query: 375 NDVLGHSFGDDLLRQVAARLDAQLLYPGAKVARLGGDEFAILLPGVDLAGALDQAAHILR 434 ND LGH GD LL +VA RL + L VARLG DEFA+ L G+D A +L Sbjct: 290 NDTLGHVAGDMLLVEVAMRL-RRALREQDVVARLGADEFAVALFGIDKDSPAYVAQKLLA 348 Query: 435 ALEQPLSLQEQTVDLGAGIGVAGYPAHGDSSEVLLSRAEVAMYTAKRKGN----GVTIYD 490 + P + + +GA IGV+ YP + +E LL A++AMY AK+ G G Y Sbjct: 349 LFDDPFFIDGHELRVGASIGVSLYPENATDTETLLRLADIAMYRAKQGGENNEGGYACYS 408 Query: 491 PAIDQSSQESLSLLSELRRALERDEFMLYLQPKLDLATGAVVGGEALVRWVHPEKGFIGP 550 +++++ + L + + L A E +E +LY QPK+D+ T A+ G EALVRW HPE+G + P Sbjct: 409 EEMNRNTLDLLRIETGLLHAFEYNELLLYYQPKVDMQTLAITGAEALVRWQHPERGLLLP 468 Query: 551 DNFIPFAETTGFIRMLTIWMMEKVAGLSARFKGGG 585 FIP AE TG I L+ W+++ V + +K G Sbjct: 469 GEFIPIAEETGLIVQLSDWVLDAVCRQARAWKDAG 503 >gi|300311036|ref|YP_003775128.1| signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 750 Score = 181 bits (460), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 111/278 (39%), Positives = 157/278 (56%), Gaps = 11/278 (3%) Query: 312 TMRSEIASRELKISRLAYQDQLTGLPNRVQFGDLLREAIGQAQ--ALPGGSCHILMMDLD 369 T R E+ E K+ A D L+GLPNR GD+L+ + A+ A P ++ +DLD Sbjct: 300 TRRKEV---EFKLRHQANYDPLSGLPNRRLLGDMLQREVASAKKHAYPLA---VVFIDLD 353 Query: 370 RFQQVNDVLGHSFGDDLLRQVAARLDAQLLYPGAKVARLGGDEFAILLPGVDLAGALDQA 429 RF+ VND LGHS GD LL A R+ A + VARL GDEF +LP ++ + AL + Sbjct: 354 RFKDVNDSLGHSVGDQLLVVAAQRVRASV-REHDLVARLAGDEFVAVLPRLEASAALTRI 412 Query: 430 AH-ILRALEQPLSLQEQTVDLGAGIGVAGYPAHGDSSEVLLSRAEVAMYTAKRKG-NGVT 487 I+ AL +P L E V + A +G+A YP D+ L+ A+ AM+ AK G NG+ Sbjct: 413 GDDIVAALAEPFQLGEHRVRISASVGIAIYPHDADTVTTLIGCADQAMFAAKGGGRNGLA 472 Query: 488 IYDPAIDQSSQESLSLLSELRRALERDEFMLYLQPKLDLATGAVVGGEALVRWVHPEKGF 547 + + + S E LL++L AL+ +F L+ QP L+L +G + EALVRW HP G Sbjct: 473 FFTEDMREKSFERRRLLNDLYDALQEGQFELHFQPALELQSGVIHKAEALVRWRHPAFGM 532 Query: 548 IGPDNFIPFAETTGFIRMLTIWMMEKVAGLSARFKGGG 585 I PD FIP AE +G I L W++++ A +S R+ G Sbjct: 533 ISPDRFIPLAEESGLIFDLGRWILQEAARVSVRWNAQG 570 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,692,247 Number of extensions: 71177 Number of successful extensions: 448 Number of sequences better than 1.0e-08: 44 Number of HSP's gapped: 364 Number of HSP's successfully gapped: 44 Length of query: 588 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 498 Effective length of database: 1,193,720 Effective search space: 594472560 Effective search space used: 594472560 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)