BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0040.p (179 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane... 343 3e-96 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 159 2e-40 gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 157 4e-40 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 151 3e-38 gi|300312026|ref|YP_003776118.1| signal transduction protein con... 149 2e-37 >gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 797 Score = 343 bits (881), Expect = 3e-96, Method: Compositional matrix adjust. Identities = 162/178 (91%), Positives = 171/178 (96%) Query: 2 KFSVNLSTRDLLDQELPNAFGAILERHRLTPAALCLEITESAIMDDPARAQSTLDRLAAM 61 KFSVNLSTRDLLDQELP AF AIL+RHRL PAALCLEITESAIMDDPARAQ+TL+RL+ M Sbjct: 620 KFSVNLSTRDLLDQELPTAFAAILQRHRLQPAALCLEITESAIMDDPARAQNTLERLSNM 679 Query: 62 GFALSIDDFGTGYSSLAYLKRLPVDELKIDKSFVMKMAQDQDDAKIVRSTVDLGHNLGLR 121 GFALSIDDFGTGYSSLAYLKRLPVDELKIDKSFVMKMAQDQDDAKIVRSTVDLGHNLGLR Sbjct: 680 GFALSIDDFGTGYSSLAYLKRLPVDELKIDKSFVMKMAQDQDDAKIVRSTVDLGHNLGLR 739 Query: 122 VVAEGVESEEVWFLLRQMGCDQGQGYFMGKPMPVERFLEWLQQWRAPEMLDQSENVAI 179 VVAEGVESEEVWFLLRQMGCDQGQGYFMGKPMPV+ FL W +QWRAP+MLDQSEN+A+ Sbjct: 740 VVAEGVESEEVWFLLRQMGCDQGQGYFMGKPMPVQHFLAWREQWRAPDMLDQSENMAV 797 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 159 bits (401), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 79/161 (49%), Positives = 107/161 (66%), Gaps = 1/161 (0%) Query: 4 SVNLSTRDLLDQELPNAFGAILERHRLTPAALCLEITESAIMDDPARAQSTLDRLAAMGF 63 +VNLS R L +Q LP+ +L + L + L LEITE+ +M D ++ +TL L +G Sbjct: 531 AVNLSPRQL-NQSLPDFVAGVLAQSGLAASWLELEITENVVMKDAEKSVATLHALKRLGL 589 Query: 64 ALSIDDFGTGYSSLAYLKRLPVDELKIDKSFVMKMAQDQDDAKIVRSTVDLGHNLGLRVV 123 +S+DDFGTGYSSL+YL+R PVD LKIDKSFV + +D D A IV++ + L H L LRV+ Sbjct: 590 QISVDDFGTGYSSLSYLRRFPVDALKIDKSFVRDIVRDADSAAIVKAIISLAHILNLRVI 649 Query: 124 AEGVESEEVWFLLRQMGCDQGQGYFMGKPMPVERFLEWLQQ 164 AEGVE EE L++ CD+ QGY+ GKPM V F W+ + Sbjct: 650 AEGVEDEEQHAFLKENACDEVQGYYFGKPMEVAVFTAWMSE 690 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 157 bits (398), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 83/161 (51%), Positives = 103/161 (63%) Query: 4 SVNLSTRDLLDQELPNAFGAILERHRLTPAALCLEITESAIMDDPARAQSTLDRLAAMGF 63 SVNLS D L LERH+L P L LE+TES M D + TL ++A +G Sbjct: 529 SVNLSALQFADPHLLEVVRTTLERHQLPPPCLTLEVTESTAMHDTEASLQTLRQIADLGV 588 Query: 64 ALSIDDFGTGYSSLAYLKRLPVDELKIDKSFVMKMAQDQDDAKIVRSTVDLGHNLGLRVV 123 +SIDDFGTGYSSL YLKRLP +ELKID+ FV ++ QD +DA IV + V LG L LR+V Sbjct: 589 EISIDDFGTGYSSLLYLKRLPANELKIDRGFVRELRQDGEDAAIVSAIVALGRTLNLRIV 648 Query: 124 AEGVESEEVWFLLRQMGCDQGQGYFMGKPMPVERFLEWLQQ 164 AEGVE+ E L GCD QGY MG+PM E FL+ +++ Sbjct: 649 AEGVETVEQQDFLTAAGCDALQGYLMGRPMTPELFLQTMRE 689 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 151 bits (382), Expect = 3e-38, Method: Compositional matrix adjust. Identities = 81/163 (49%), Positives = 104/163 (63%) Query: 4 SVNLSTRDLLDQELPNAFGAILERHRLTPAALCLEITESAIMDDPARAQSTLDRLAAMGF 63 SVNLS L ++L ++ +LE L P +L LE+TES +M D A L L +G Sbjct: 783 SVNLSVGQFLQKDLVSSIADVLEDTGLPPESLELELTESLMMTDVEHAIGILAGLKKLGV 842 Query: 64 ALSIDDFGTGYSSLAYLKRLPVDELKIDKSFVMKMAQDQDDAKIVRSTVDLGHNLGLRVV 123 LSIDDFGTGYSSLAYLKR P+D LKID+SFV + D DDA I S + L +L LRV+ Sbjct: 843 RLSIDDFGTGYSSLAYLKRFPIDVLKIDRSFVRDITIDADDAAITASIISLARHLKLRVI 902 Query: 124 AEGVESEEVWFLLRQMGCDQGQGYFMGKPMPVERFLEWLQQWR 166 AEGVE++E LR GCD+ QGY P+P ++F + L+Q R Sbjct: 903 AEGVETDEQLDYLRHYGCDEIQGYLFSPPVPADKFEQILRQDR 945 >gi|300312026|ref|YP_003776118.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1106 Score = 149 bits (375), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 71/161 (44%), Positives = 106/161 (65%) Query: 2 KFSVNLSTRDLLDQELPNAFGAILERHRLTPAALCLEITESAIMDDPARAQSTLDRLAAM 61 + S+NL+ D+ L + +++RH P+ L EITES +M + RA + +D + A Sbjct: 932 RISINLTGSQFGDERLLDDIKEVVDRHGTPPSCLEFEITESMVMHNRERAIAIMDGIRAE 991 Query: 62 GFALSIDDFGTGYSSLAYLKRLPVDELKIDKSFVMKMAQDQDDAKIVRSTVDLGHNLGLR 121 GF +SIDDFGTGYSSLAYLKR PV+ LKIDKSF+ + + + + IV++ + + H L L+ Sbjct: 992 GFTISIDDFGTGYSSLAYLKRFPVNTLKIDKSFINDIPDNPNSSAIVQAIIAMSHALNLK 1051 Query: 122 VVAEGVESEEVWFLLRQMGCDQGQGYFMGKPMPVERFLEWL 162 VV EGVE+E+ LR+ G D+ QGY+ KP+P +F++ L Sbjct: 1052 VVTEGVETEKQLQALREFGSDEYQGYYFSKPVPYAQFVQLL 1092 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 583,153 Number of extensions: 22956 Number of successful extensions: 104 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 102 Number of HSP's successfully gapped: 18 Length of query: 179 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 100 Effective length of database: 1,245,805 Effective search space: 124580500 Effective search space used: 124580500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)