BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0021.0008.p
         (213 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311982|ref|YP_003776074.1| 2-keto-4-pentenoate hydratase [...   341   2e-95
gi|300312647|ref|YP_003776739.1| 2-keto-4-pentenoate hydratase [...    54   8e-09

>gi|300311982|ref|YP_003776074.1| 2-keto-4-pentenoate hydratase
           [Herbaspirillum seropedicae SmR1]
          Length = 262

 Score =  341 bits (875), Expect = 2e-95,   Method: Compositional matrix adjust.
 Identities = 168/209 (80%), Positives = 182/209 (87%)

Query: 4   PLAGWKNAATGPGGQQLLKVDAPLAGRLLAERIHRSGARITLGDNLMRVAEVEVAFRMGS 63
           PLAGWK  ATGPGGQQLL VD P+AGRLLAER+HR+GA +TLGDNLM V E EV FRM S
Sbjct: 53  PLAGWKIGATGPGGQQLLNVDGPMAGRLLAERMHRNGAHVTLGDNLMSVIEAEVVFRMRS 112

Query: 64  TLAPRNTPYTQEEVMAAVATVHPAIEIPDTRYDLFREVGAAQLIADNSCADQFVLGAPAS 123
            L PR  PY+QEEVM AV +VHP IEIPDTRY  FREVGA QLIADN CADQFVLGAP S
Sbjct: 113 ALPPRTQPYSQEEVMEAVGSVHPGIEIPDTRYQQFREVGAYQLIADNCCADQFVLGAPTS 172

Query: 124 IDWRSLDLAALATSATIEGGERRTGSGAQVLGDPRVALTWLANELREYGLTLAAGQLVAT 183
           +DWR LDLAALATSA IEGGE+R+GSGAQVLGDPR+ALTWL NELR++GLTLAAGQLVAT
Sbjct: 173 MDWRHLDLAALATSAFIEGGEQRSGSGAQVLGDPRIALTWLVNELRQHGLTLAAGQLVAT 232

Query: 184 GSTIVPMPVRAGQTVLAELGVLGSVVVHL 212
           GSTIVPMPV+AGQTV A+LGVLGSV VHL
Sbjct: 233 GSTIVPMPVQAGQTVRADLGVLGSVTVHL 261


>gi|300312647|ref|YP_003776739.1| 2-keto-4-pentenoate hydratase
           [Herbaspirillum seropedicae SmR1]
          Length = 247

 Score = 54.3 bits (129), Expect = 8e-09,   Method: Compositional matrix adjust.
 Identities = 59/205 (28%), Positives = 83/205 (40%), Gaps = 9/205 (4%)

Query: 4   PLAGWKNAATGPGGQQLLKVDAPLAGRLLAERIHRSGARITLGDNLMRVAEVEVAFRMGS 63
           P+ GWK  + G GG        P    L A  +  SG  +      MR  EVE+A R+G 
Sbjct: 46  PVGGWKVGSKGDGG-------TPGCAPLPASGVLPSGTTLAGPQWRMRGLEVELALRVGR 98

Query: 64  TLAPRNTPYTQEEVMAAVATVHPAIEIPDTRYDLFREVGAAQLIADNSCADQFVLGAPAS 123
              P      + E+      + PAIE+ +TR            +AD       V GAP +
Sbjct: 99  DYDPGEQLPDEAELRTVFDAILPAIEVVETRLGHPPCTNPWAGMADLQSHGALVTGAPQA 158

Query: 124 IDWRSLDLAALATSATIEGGERRTGSGAQVLGDPRVALTWLANELREYGLTLAAGQLVAT 183
           +    L L  +  S +I+G +    +G     D    L WLA      G    AGQ++ T
Sbjct: 159 LP-DLLSLRQIEASLSIDGQQVSHTTGGNP-ADLWPLLGWLALHCARRGQPWRAGQIITT 216

Query: 184 GSTIVPMPVRAGQTVLAELGVLGSV 208
           GS       R G+ V A L  +G+V
Sbjct: 217 GSCTGLQMARGGRRVEAALHGIGAV 241


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.318    0.134    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 700,542
Number of extensions: 28475
Number of successful extensions: 131
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 129
Number of HSP's successfully gapped: 2
Length of query: 213
Length of database: 1,619,870
Length adjustment: 81
Effective length of query: 132
Effective length of database: 1,236,335
Effective search space: 163196220
Effective search space used: 163196220
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 128 (53.9 bits)