BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0021.0006.p
         (303 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311008|ref|YP_003775100.1| esterase/lipase/thioesterase fa...   484   e-138
gi|300312950|ref|YP_003777042.1| lipase [Herbaspirillum seropedi...    73   4e-14
gi|300310447|ref|YP_003774539.1| esterase [Herbaspirillum serope...    61   1e-10
gi|300311178|ref|YP_003775270.1| lipase [Herbaspirillum seropedi...    57   2e-09

>gi|300311008|ref|YP_003775100.1| esterase/lipase/thioesterase
           family protein [Herbaspirillum seropedicae SmR1]
          Length = 302

 Score =  484 bits (1247), Expect = e-138,   Method: Compositional matrix adjust.
 Identities = 241/300 (80%), Positives = 266/300 (88%), Gaps = 5/300 (1%)

Query: 1   MHVLGKAGLAFAGAVLILGGVVACSPLSAINAVTPASSYVKTEGVAYANTDNPRQKLDIY 60
           M V GKA L  AGA  +LG ++ACSPL+AINAVTPAS+Y+KTEGVAYA +DN RQ+LDIY
Sbjct: 1   MPVPGKAALVLAGATALLGSLIACSPLTAINAVTPASTYLKTEGVAYAGSDNARQQLDIY 60

Query: 61  RPANAPATGSGP--GLPVVVFFYGGSWNEGSRKDYAFVGEALSSRGFIAVLPDYRVYPEV 118
           RPA+   T S P  G PVVVFFYGGSWN+GSRKDYAFVGEAL+SRG IAVLPDYR+YPEV
Sbjct: 61  RPAD---TASAPPQGRPVVVFFYGGSWNQGSRKDYAFVGEALASRGVIAVLPDYRLYPEV 117

Query: 119 RYPDFVKDSAQAVAWTLRSVRELGGDPQRVFVMGHSAGAYNAAMVALDARWLRAAGASPA 178
            YPDF++DSAQAVAWTLRS++ LGGDPQRVFVMGHSAGAYNAAM+ALDARWL AAGA+PA
Sbjct: 118 SYPDFLQDSAQAVAWTLRSLQALGGDPQRVFVMGHSAGAYNAAMMALDARWLDAAGATPA 177

Query: 179 QLRGWIGLAGPYDFLPIDNEDVKPVFFAPDSPPDSQPVRHVEAGAPPALLIASHKDKLVG 238
           QLRGWIGLAGPYDFLPI+N DVKPVF  PDSPPDSQPVRHV+A APPALLIASH+DKLV 
Sbjct: 178 QLRGWIGLAGPYDFLPIENPDVKPVFHFPDSPPDSQPVRHVQASAPPALLIASHQDKLVS 237

Query: 239 AERNTGGLAKLLRAQQVPVVERYFDNTSHASLVGSLSRPLRFIAPTLEEVSQFIENPPPR 298
           A RNTGGLA LLR   VPVVE YFDNTSHASLVGSL+RPLRFIAPTL+EVS+FI+ PPPR
Sbjct: 238 ATRNTGGLAALLRQHGVPVVETYFDNTSHASLVGSLARPLRFIAPTLDEVSRFIDAPPPR 297


>gi|300312950|ref|YP_003777042.1| lipase [Herbaspirillum seropedicae
           SmR1]
          Length = 289

 Score = 72.8 bits (177), Expect = 4e-14,   Method: Compositional matrix adjust.
 Identities = 44/162 (27%), Positives = 73/162 (45%), Gaps = 21/162 (12%)

Query: 36  ASSYVKTEGVAYANTDNPRQKLDIYRPANAPATGSGPGLPVVVFFYGGSWNEGSRKDYAF 95
           A + +    ++Y   D P Q+ D YR         G   P++VF +GG W  G ++D  F
Sbjct: 50  ADTLLTESDISYG--DAPLQRFDFYR-------AEGAQRPLLVFIHGGYWQGGDKRDIGF 100

Query: 96  VGEALSSRGFIAVLPDYRVYPEVRYPDFVKDSAQAVAWTLRSVRELGGDPQRVFVMGHSA 155
           +       G    + +Y + P+ R  D VK+    ++   +    LG D  R+ +MGHSA
Sbjct: 101 IAAPYVKAGISVAVINYSLAPQARIEDMVKEVQACLSTIAQQAERLGIDVDRISLMGHSA 160

Query: 156 GAYNAAMVALDARWLRAAGASPAQL--RGWIGLAGPYDFLPI 195
           G + AA VA          A P ++  +    ++G +D  P+
Sbjct: 161 GGHLAAFVA----------AQPGRMPVQAVFAISGVFDLAPL 192


>gi|300310447|ref|YP_003774539.1| esterase [Herbaspirillum
           seropedicae SmR1]
          Length = 277

 Score = 60.8 bits (146), Expect = 1e-10,   Method: Compositional matrix adjust.
 Identities = 38/123 (30%), Positives = 60/123 (48%), Gaps = 9/123 (7%)

Query: 73  GLPVVVFFYGGSWNEGSRKDYAFVGEALSSRGFIAVLPDYRVYPEVRYPDFVKDSAQAVA 132
           G P++VF +GG W    +  ++ +      +     L +YR+ P+VR  D   D  +A+ 
Sbjct: 68  GRPLLVFIHGGYWRSLDKHQFSHLALPYLEQDINVALINYRLAPQVRMGDIAADCGRALR 127

Query: 133 WTLRSVRELGGDPQRVFVMGHSAGAYNAAMVALDARWLRAAGASPAQLRGWIGLAGPYDF 192
                   L  D   +++MGHSAGA+ A ++A       A  A+P  +RG   L+G YD 
Sbjct: 128 LLHAKAPALRVDANAIWLMGHSAGAHLACVIA-------ALRAAP--VRGVCALSGLYDL 178

Query: 193 LPI 195
            PI
Sbjct: 179 EPI 181


>gi|300311178|ref|YP_003775270.1| lipase [Herbaspirillum seropedicae
           SmR1]
          Length = 340

 Score = 57.0 bits (136), Expect = 2e-09,   Method: Compositional matrix adjust.
 Identities = 38/119 (31%), Positives = 59/119 (49%), Gaps = 5/119 (4%)

Query: 51  DNPRQKLDIYRPANAPATGSGPGLPVVVFFYGGSWNEGSRKDYA-FVGEALSSRGFIAVL 109
           D  + KL I RPA +    +   LP  +FF+GG W  G    +   V + +++ G +AV 
Sbjct: 83  DGQQIKLTIVRPAGS----AKKTLPAFMFFHGGGWVLGDFPTHERLVRDLVANSGAVAVF 138

Query: 110 PDYRVYPEVRYPDFVKDSAQAVAWTLRSVRELGGDPQRVFVMGHSAGAYNAAMVALDAR 168
            +Y   PE  Y   +  +  A  W     +E+  D +R+ V G+S G   AA+VAL A+
Sbjct: 139 VNYTPSPEAHYGVAINQAYAATKWVAEHGKEINVDGKRLAVAGNSVGGNMAAVVALMAK 197


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.318    0.136    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,162,079
Number of extensions: 55296
Number of successful extensions: 179
Number of sequences better than 1.0e-08: 5
Number of HSP's gapped: 176
Number of HSP's successfully gapped: 5
Length of query: 303
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 218
Effective length of database: 1,217,395
Effective search space: 265392110
Effective search space used: 265392110
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 130 (54.7 bits)