BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0021.0002.p
         (550 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312549|ref|YP_003776641.1| hemolysin activation/secretion ...   465   e-132
gi|300310625|ref|YP_003774717.1| hemolysin activation translocat...   310   2e-85

>gi|300312549|ref|YP_003776641.1| hemolysin activation/secretion
           protein [Herbaspirillum seropedicae SmR1]
          Length = 584

 Score =  465 bits (1197), Expect = e-132,   Method: Compositional matrix adjust.
 Identities = 245/468 (52%), Positives = 309/468 (66%), Gaps = 18/468 (3%)

Query: 55  PDVNL-GTPAASAMAQDFPEQEQPCRRITAIALEGEQAERFRFALDSVTGERGAVGRCLG 113
           PDV L   P A+A A   P QE PC  +  + L+G  + RF + LD      G  GRCLG
Sbjct: 66  PDVRLPAQPGAAAEA--VPAQESPCFAVHRVVLDGPGSARFDWLLDLPEANPG--GRCLG 121

Query: 114 SQGVNLVLARVESAIVARGYTTTRVLARPQDLSTGRLVLSVVPGRVRHIRFAPDADARGT 173
            QG+N V++ +++AIVARGY TTR+LA+PQD+++G L L++VPG++R IRFA +AD R T
Sbjct: 122 VQGINAVMSALQNAIVARGYVTTRILAQPQDIASGELRLTIVPGKIRAIRFADNADPRAT 181

Query: 174 AFNALPISPGDILNLRDIEQGLENFKRLPTVQADIKITPSEDKGAEAGDSDLVIQYQQAF 233
            +NALP SPGD+LNLRDIEQGLEN KR+PT +AD++I PSE K A  GDSDLVI YQQ  
Sbjct: 182 QWNALPASPGDLLNLRDIEQGLENLKRVPTAEADVQIAPSEGKDAAPGDSDLVISYQQGI 241

Query: 234 PFRLTASLDDGGAKATGKYLAGLTLSYDNWWTLNDLFYVSVSRSL--NHYGDHGSHAYTL 291
           P RLT S+DD G KATGKY    T+S DNW TLNDLFY+S S  L     G  GS +  +
Sbjct: 242 PIRLTLSVDDSGTKATGKYQGSATVSLDNWLTLNDLFYISFSHDLGGGDPGARGSQSQVV 301

Query: 292 HYSLPLGNWMAAVTMSGNRYHQTVAGAFENFVYSGRSETTEFKLSRLLHRDAAGKTTAFG 351
           HYSLPLG W  A T S NRYHQ VAG  + ++YSG+SET E KLSRL++RDA  KTT   
Sbjct: 302 HYSLPLGYWALAFTNSSNRYHQAVAGINQTYLYSGQSETNELKLSRLVYRDAVRKTTLSF 361

Query: 352 KALLRGGGGGGGKAYNAIDELEVGAQQRRTTAWEAGLNHRQFLGDAIADATLAYRRSLDT 411
           K  +R        + N +D+ EV  Q+RR   WE GL HR+F+G+   D  LAY+R    
Sbjct: 362 KGFVR-------SSSNYVDDTEVQVQRRRVAGWEMGLAHREFIGEGTLDLALAYKRGTGA 414

Query: 412 QGAEPSSPFDLPQIGTRYGLWLLDASVNAPFQL----AGARLRYSSTLRAQWNRGSLPPQ 467
             A  +      +  +R+ L   D ++NAPF L        +RY+   RAQWNR  L PQ
Sbjct: 415 FDAMAAPEEAFGEGTSRFRLMTADLNLNAPFALPMPWGKQAMRYTLGARAQWNRTPLTPQ 474

Query: 468 DQFSIGGRYTVRGFDGELTLQAERGWFLRNELALGLGESGQEVFAGID 515
           D+FS+GGRY+VRGFDGE  L AERGW++RNELA  LG S QE++AG+D
Sbjct: 475 DKFSLGGRYSVRGFDGESNLLAERGWYVRNELAAALGASKQEIYAGLD 522


>gi|300310625|ref|YP_003774717.1| hemolysin activation translocator
           protein [Herbaspirillum seropedicae SmR1]
          Length = 581

 Score =  310 bits (794), Expect = 2e-85,   Method: Compositional matrix adjust.
 Identities = 190/451 (42%), Positives = 255/451 (56%), Gaps = 27/451 (5%)

Query: 71  FPEQ-EQPCRRITAIALEGEQAERFRFALDSVTGERGAVGRCLGSQGVNLVLARVESAIV 129
           FPEQ  +  RR    AL   Q + F F    + G  G+   CLG QG+  +L RV   I+
Sbjct: 90  FPEQVSEAVRRTGGFAL---QMDPFHFIQTYLAGYAGS---CLGQQGIETILRRVTGQIL 143

Query: 130 ARGYTTTRVLARPQDLSTGRLVLSVVPGRVRHIRFAPDADARGTAFNALPISPGDILNLR 189
            RGY+TTRV    QDLSTG+L +++VPG +  IRF  D     +  +A P   G +LNLR
Sbjct: 144 ERGYSTTRVGIGQQDLSTGQLSITLVPGIIHAIRF-KDPAITASYRSAFPTQAGALLNLR 202

Query: 190 DIEQGLENFKRLPTVQADIKITPSEDKGAEAGDSDLVIQYQQAFPFRLTASLDDGGAKAT 249
           D+EQGLE  KR+ +   D++I P++      G SD+V++ ++  P++LT SLDD GAK+T
Sbjct: 203 DLEQGLEQMKRVASQDVDMQILPTD----VPGQSDVVLEVKRIKPWKLTLSLDDSGAKST 258

Query: 250 GKYLAGLTLSYDNWWTLNDLFYVSVSRSLNHYGD-HGSHAYTLHYSLPLGNWMAAVTMSG 308
           GKY AG+ L+ D+   +NDLF    S   +  G   G+      YS P G W   V+ S 
Sbjct: 259 GKYQAGINLAIDDLLGINDLFNAGFSSDADRMGQARGTTGNNFSYSAPAGYWTFGVSGST 318

Query: 309 NRYHQTVAGAFENFVYSGRSETTEFKLSRLLHRDAAGKTTA---FGKALLRGGGGGGGKA 365
           +RYHQ +AG F+ FV SG SE  E ++ +L  RDA  K +     GK   R         
Sbjct: 319 SRYHQRIAGLFQTFVSSGHSENLEVRVQKLFQRDAQQKNSLQLRIGKRWSR--------- 369

Query: 366 YNAIDELEVGAQQRRTTAWEAGLNHRQFLGDAIADATLAYRRSLDTQGAEPSSPFDLPQI 425
            + ID+ E+  Q+R TT  E G  HR + GD   DATLA R  +        +P  L   
Sbjct: 370 -SYIDDTEIEVQRRNTTFAELGWMHRHYFGDVQLDATLANRWGVSWFNGAADAPDRLSDG 428

Query: 426 GT-RYGLWLLDASVNAPFQLAGARLRYSSTLRAQWNRGSLPPQDQFSIGGRYTVRGFDGE 484
            T RY +  +DA+V APF+L G  L Y  TLR Q +R  L   DQF+IG RYTVRGFDGE
Sbjct: 429 PTYRYSMQTIDATVVAPFKLGGQSLTYVGTLRGQHSRSRLYLTDQFAIGSRYTVRGFDGE 488

Query: 485 LTLQAERGWFLRNELALGLGESGQEVFAGID 515
           LTL +E G FLRN+L + LG +G  ++AG+D
Sbjct: 489 LTLASESGVFLRNDLEIALGSTGAALYAGLD 519


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.318    0.134    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,705,028
Number of extensions: 73153
Number of successful extensions: 217
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 208
Number of HSP's successfully gapped: 2
Length of query: 550
Length of database: 1,619,870
Length adjustment: 90
Effective length of query: 460
Effective length of database: 1,193,720
Effective search space: 549111200
Effective search space used: 549111200
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 133 (55.8 bits)