BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0001.p (1560 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312548|ref|YP_003776640.1| filamentous hemagglutinin outer... 401 e-112 gi|300310626|ref|YP_003774718.1| filamentous hemagglutinin prote... 312 1e-85 >gi|300312548|ref|YP_003776640.1| filamentous hemagglutinin outer membrane protein [Herbaspirillum seropedicae SmR1] Length = 2685 Score = 401 bits (1030), Expect = e-112, Method: Compositional matrix adjust. Identities = 285/653 (43%), Positives = 396/653 (60%), Gaps = 50/653 (7%) Query: 1 MNRQRYKLVFNRHRGQLMAVAEIASSIQHHGRTSANGAAMPDLLPSALRFSLLAVALALA 60 MN+ Y+++FN+ RG LMAVAE + + LP A R S + +A+ LA Sbjct: 1 MNQLCYRIIFNKTRGLLMAVAETTRACAGKRAVGSRPGVALGGLPMA-RLSWIGLAVLLA 59 Query: 61 MNTV------AHAQILGDRNAPRNQQPTVLATPNGVPVVNIQTPGAAGVSLNSYRQFDVG 114 + A+AQI+ D NAPR QQP V+ T NG+P VNIQTP AAGVS N+Y QFDV Sbjct: 60 TGALVTTMPRAYAQIIADPNAPRAQQPVVVNTANGIPQVNIQTPSAAGVSRNTYSQFDVN 119 Query: 115 PNGAILNNAQGTTATQLGGYVAGNPWLATGAARVIVNQVNSANPSYLRGYLEVAGARAQV 174 GAILNN++ TQLGG+V NP LA G ARVI+N+VNS+NPS LRG++EVAG RAQV Sbjct: 120 RAGAILNNSRSDVQTQLGGWVQRNPNLANGTARVILNEVNSSNPSMLRGFVEVAGDRAQV 179 Query: 175 VVANPAGITCSGCGFINANRATITTGVPQIVNGNLDSYRVSSGAVRIEGNGMDASRTDYA 234 ++ANP+G+TC GCGFINA+R+T+TTG P I NG+LD Y V G V I+GNG++ S++ + Sbjct: 180 IIANPSGVTCDGCGFINASRSTLTTGTPIINNGSLDGYLVRGGVVSIKGNGLNDSQSSFT 239 Query: 235 EIIARAVQVNAGIWAPELKVSAGLNTVSADHASVVPGVAASDAAPAVAIDVAQLGGMYAG 294 ++IARA ++NAGIW+ ELKV+ G N V+AD+ +V V+A A P IDVAQLGGMYA Sbjct: 240 DLIARAAKINAGIWSKELKVTVGTNQVNADNTTVT-AVSADPAKPQFGIDVAQLGGMYAN 298 Query: 295 HIYLTSTEHGVGVRNAGNIGASVGQAVVTADGRLENSGSLTATGLLAVQTTGGISNAGEL 354 I L TE GVGVRNAG IGA+ G+ +TA GR+ENSGSL AT L V+ TG G++ Sbjct: 299 KIVLVGTESGVGVRNAGAIGAAAGELTLTAAGRIENSGSLAATASLGVRATG---QGGDI 355 Query: 355 GSKSAASLNTTALDNSGSIASAGALTVQASGALQNTGSIGGEGNVQIAAASIDNRNSIGS 414 +++ +SA A+ +QA G + N+ +I + V + AAS+ N +I + Sbjct: 356 ------------VNSGSVASSAAAVKLQADGGINNSNTISAQSGVTLTAASLQNSGAISA 403 Query: 415 ST-TLAVQAAGSLSNSGTLQATADASINAASVDNSGTVRAAQGALNVSSSARTGNSGRME 473 +T + V G+++NSG +QA D SI+A QG +++A N+G +E Sbjct: 404 ATGNVEVDTHGAVNNSGKVQAAGDISISATQ---------PQG----NAAADISNTGNIE 450 Query: 474 AAQAVNLQV-GSLANSGGVNAGERLRIEAAQSISNDGTLA--ANGPVAVQAASVANRGTL 530 AA + L G L+N G + A + +R Q + N+G++ +N +Q V Sbjct: 451 AAGQLGLTADGMLSNDGSITA-QSIR-AGVQGLRNNGSIGTRSNAAFTLQQGLVQGANGR 508 Query: 531 ASSADAVNVTTSGTLANQGQVNAAQALTLTAAGIDNRSG-KLSANDIRIDSQNQSLDNRQ 589 S+ +N+T G N G+V A LTL+AA I NRSG L A DI + + N+ LDN Sbjct: 509 LSAVSDLNLTMQGDYDNAGRVQ-ANTLTLSAANIGNRSGAALQAADITLTALNR-LDN-A 565 Query: 590 GNIAAQRDVRIASGALDNGSGNIVAQGKLDIRSGDLGNDG--GLLQAGDALSL 640 G I+A R + I + ++ N S I+ + L + + L N G LL AG+ +SL Sbjct: 566 GEISANRQLNITADSILN-SATILGK-DLVVNARTLENTGANALLGAGNGMSL 616 Score = 116 bits (291), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 77/203 (37%), Positives = 111/203 (54%), Gaps = 23/203 (11%) Query: 1358 NFQLDITPTSGAGPNRQNSVRAVASSVSGASGASAGAASIRIANLDLTLPNNALFRINAG 1417 N + D+T T+G+G SV +V + S GA+A A + + PN +G Sbjct: 993 NIRSDLTLTTGSG----QSVGSVLNDASARPGATAPDAPVPVR------PNPMFVPATSG 1042 Query: 1418 PAQRFLVQTDPQFVGTHSWLSSDFMLNQLGLTASVRQ--LGDGFYEQQLIQRQVQQITGQ 1475 +L++T QF +WLSSD++L L + S Q LGDGFYEQ++++ Q+ Q+TG+ Sbjct: 1043 ---NYLLETRTQFTDRRTWLSSDYLLTALNVDPSTTQKRLGDGFYEQRMVREQMAQLTGR 1099 Query: 1476 RYLAGYSSNEAQYQALMNAGVQVAQSQRYTLGVALTDAQIAELKTDIVWLVKQTVTLADG 1535 +G ++++QYQ L+N V VAQS GVALT Q++ L +DIVWL Q V L DG Sbjct: 1100 P--SGSFNDDSQYQKLLNNAVSVAQSWNLRPGVALTPDQVSHLTSDIVWLETQKVVLPDG 1157 Query: 1536 STXAAGLYPCLQCAGDRTRHADR 1558 T L P L A H D+ Sbjct: 1158 RTEEV-LVPKLYLA-----HIDK 1174 >gi|300310626|ref|YP_003774718.1| filamentous hemagglutinin protein [Herbaspirillum seropedicae SmR1] Length = 2658 Score = 312 bits (800), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 267/732 (36%), Positives = 389/732 (53%), Gaps = 75/732 (10%) Query: 48 LRFSLLAVALALAMNTVAHAQILGDRNAPRNQQPTVLATPNGVPVVNIQTPGAAGVSLNS 107 LR ++ A+ + L + + QI+ D NA ++P V T NG P+V I TP AAGVS N Sbjct: 19 LRRAMAALMIMLCVVPPGYPQIVADPNAGAGKRPVVDNTANGRPLVQIATPSAAGVSHNQ 78 Query: 108 YRQFDVGPNGAILNNAQGTTATQLGGYVAGNPWLATGAARVIVNQVNSANPSYLRGYLEV 167 Y QF+VGPNGAILNNA+ TQ GGYV NP L GAARVI+N+V S +PS LRGY EV Sbjct: 79 YSQFNVGPNGAILNNARDVVLTQQGGYVGANPNLQGGAARVILNEVTSTSPSQLRGYTEV 138 Query: 168 AGARAQVVVANPAGITCSGCGFINANRATITTGVPQI-VNGNLDSYRVSSGAVRIEGNGM 226 AG RA+V++ANP GI+C GCGFIN +R +TTGVP NG+L+++RVS GA++I G+ Sbjct: 139 AGQRAEVIIANPNGISCDGCGFINTSRGVLTTGVPLFGGNGSLEAFRVSRGAIQIGSGGL 198 Query: 227 DASRTDYAEIIARAVQVNAGIWAPELKVSAGLNTVSADHASVVPGVAASDAAPAVAIDVA 286 + + TD ++IAR+VQVN +W L + G N V+ + +A AP + +DVA Sbjct: 199 NGANTDQLDLIARSVQVNGELWGNRLNLVTGANRVNYGDLGLSV-IAGEGNAPTIGVDVA 257 Query: 287 QLGGMYAGHIYLTSTEHGVGVRNAGNIGASVGQAVVTADGRLENSGSLTAT--------- 337 ++GGMYA I L TE+GVGVR+ G + A G +++ G++ SG +A+ Sbjct: 258 RMGGMYAQKIMLVGTENGVGVRSLGTLAAQAGSFTLSSQGQIALSGKTSASDDIVLKSAV 317 Query: 338 -----GLLA----VQTTGG-ISNAGELGSK-----SAASLNTT-----ALDNSGSIASAG 377 G+L Q GG I NAG L ++ +AA L +T +D +G++A AG Sbjct: 318 GTSNDGVLYAGRNAQLQGGVIENAGTLSAQGNLDITAAQLRSTGTLAAGVDQAGNVAQAG 377 Query: 378 ALTVQASGALQNTGSIGGEGNVQIAAASIDNRNSIGSS-TTLAVQAAGSLSNSG------ 430 LTV ASG + TG ++Q+ ID +S S+ L QA+ +L+ G Sbjct: 378 NLTVTASGDVLATGVNRAGADLQLVGGKIDLSHSSSSAGRKLGAQASDALTLGGATVETL 437 Query: 431 ----------------TLQATADASINA-ASVDNSGTVRAAQGALNVSSSARTGNSGRME 473 L ++ADA INA A + N G + GA+NV++++ ++G + Sbjct: 438 AGDLELGGATITLDGAKLTSSADARINAVAGLSNRGGEITSAGAMNVTAASVDNSAGMIS 497 Query: 474 AAQ----AVNLQ-VGSLANSGGVNAGERLRIEAAQSISNDGTLAANGPVAVQAASVANR- 527 AA ++L+ GS++N G A AA SI G++ ANG + S+ N Sbjct: 498 AATGGAGVLSLRSAGSISNGQGRIAAGAALNLAAASIQGQGSVIANGDATLSLQSLNNEA 557 Query: 528 GTLASSADAVNVTTSGTLANQGQVNAAQALTLTAAGIDNRS-GKLSANDIRIDSQNQSLD 586 G+L S + +TTSG NQG + A AL LTA+ IDN + G +SA + + N ++ Sbjct: 558 GSLIQSNRDLRITTSGATGNQGALKAIGALNLTASQIDNAAGGDISAAATTLQALNGDIN 617 Query: 587 NRQGNIAAQRDVRIASGALDNGSGNIVAQGKLDIRSGDLGNDGGLLQAGDALSLDAGSNT 646 N G I + S AL+N G+++ G + I + L N G S+ A Sbjct: 618 N-AGKIGGNT-IATTSNALNN-QGSMIG-GDVTINADRLNNSGAAAAIAATNSVQA---F 670 Query: 647 VRNANSGSARGIV-AQGPLSVTAGALVNRGNVSSNGDISLKTTGLDNDAGVIGANGKLTV 705 VR+A + ++ + G L + A A S+ GD +T L N + I A G L V Sbjct: 671 VRSALTNQDNALIYSLGKLVIAADA-----TQSAAGDYLHRTGTLTNSSATIEAGGDLAV 725 Query: 706 EGSAVSNRGGSL 717 + N+ +L Sbjct: 726 SADTIVNKRSTL 737 Score = 209 bits (531), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 302/1068 (28%), Positives = 471/1068 (44%), Gaps = 178/1068 (16%) Query: 517 VAVQAASVANRGTLASSADAVNVTTSGTLANQGQVNAAQALTLTAAGIDNRSGKLSANDI 576 VA A + L + + V V + GTLA Q A + TL++ G SGK SA+D Sbjct: 256 VARMGGMYAQKIMLVGTENGVGVRSLGTLAAQ-----AGSFTLSSQGQIALSGKTSASDD 310 Query: 577 RIDSQNQSLDNRQGNIAAQRDVRIASGALDNGSGNIVAQGKLDIRSGDLGNDGGLLQAGD 636 + N G + A R+ ++ G ++N +G + AQG LDI + L + G L Sbjct: 311 IVLKSAVGTSN-DGVLYAGRNAQLQGGVIEN-AGTLSAQGNLDITAAQLRSTGTL----- 363 Query: 637 ALSLDAGSNTVRNANSGSARGIVAQ-GPLSVTA-GALVNRGNVSSNGDISLKTTGLDNDA 694 A +D N VAQ G L+VTA G ++ G + D+ L +D Sbjct: 364 AAGVDQAGN-------------VAQAGNLTVTASGDVLATGVNRAGADLQLVGGKIDLSH 410 Query: 695 GVIGANGKLTVEGSAVSNRGGSLQARQGVDLQVGSGSV--DNSAGLMRSDGTVNVQAGTV 752 A KL + S GG+ DL++G ++ D + +D +N AG Sbjct: 411 SSSSAGRKLGAQASDALTLGGATVETLAGDLELGGATITLDGAKLTSSADARINAVAG-- 468 Query: 753 RNDSTQGQNQGIEGATVSVSAADISNRQGAM-RGDAITLTAGTRIDNNAGLISA-TNSAS 810 +SNR G + A+ +TA + +DN+AG+ISA T A Sbjct: 469 -----------------------LSNRGGEITSAGAMNVTAAS-VDNSAGMISAATGGAG 504 Query: 811 LTDANPASRALVIDNSNGTVIAGQQTSVLAYSFTGSGRFLSQKDLRIDLVASILHTGQ-I 869 + A I N G + AG ++ A S G G ++ D + L + G I Sbjct: 505 VLSLRSAGS---ISNGQGRIAAGAALNLAAASIQGQGSVIANGDATLSLQSLNNEAGSLI 561 Query: 870 GASGDIDLRTAGTFSNAGAVGAGGTLMLTAATIDNQASGSLVGNTLKLKAT--DVHTFIN 927 ++ D+ + T+G N GA+ A G L LTA+ IDN A G + L+A D++ N Sbjct: 562 QSNRDLRITTSGATGNQGALKAIGALNLTASQIDNAAGGDISAAATTLQALNGDIN---N 618 Query: 928 RGLIDGVNTVIESSTVNNLGTGRIYGDNIAIGADVLNNQAETVNGVTSAPVIA-ARNRLD 986 G I G S+ +NN G+ + G ++ I AD LNN + V A R+ L Sbjct: 619 AGKIGGNTIATTSNALNNQGS--MIGGDVTINADRLNNSGAAAAIAATNSVQAFVRSAL- 675 Query: 987 IGAGVVNNSEHGLIYSVGDMAIGGALDANKKATGSARE----INNSSATINADGNLSIAA 1042 N ++ LIYS+G + I A DA + A G + NSSATI A G+L+++A Sbjct: 676 ------TNQDNALIYSLGKLVI--AADATQSAAGDYLHRTGTLTNSSATIEAGGDLAVSA 727 Query: 1043 GTINNTNAHLETTDQTGPGNRIVSFRVNGSSQLLDSKSAW-LYNRGSGEILDASNWRAMG 1101 TI N + L + + D +W YN WR+ G Sbjct: 728 DTIVNKRSTLVI-----------------ARDIFDGTESWQKYNY---------YWRSWG 761 Query: 1102 DEDNYRLLLPSAA--YPAERYGPPFDYSRGARGDSAVAIAYTPAYSQGAMGDADAVSYPA 1159 + P +A A PF+ A A+ Y G++ D S A Sbjct: 762 SG----MSGPDSAGLMAAVTRTLPFND----------ASAFGSRY--GSILQLDLSSNRA 805 Query: 1160 VINYKPGDRIW-SVMGVTPPAEDPGPGPGSEPRPGEACYESCVSVPVPAGVYDAWKAA-- 1216 ++ ++ +++W S + A+ E G+ C S + P V+ + + Sbjct: 806 LVQFQGSNQLWVSYKAIKQNADGSYDMTFYE---GKNCDGSTL-CPYQQMVWREYTGSRV 861 Query: 1217 YDVWKPKYDAYIAALLVLNDKITAFNNNVNSRSYREWTIYDGTEQITRTVVTKSDPGMIT 1276 D + P A L + N ++ +RE + Y+G+ + SD + Sbjct: 862 QDQFDPSRYTSPAELKGIG------TNGESAYDFRERS-YNGSN-YHDAITAMSDAAKLN 913 Query: 1277 SGGNMSLAAGTVNNYASQFIAGGTVAGDSVNGTN--------LNNTGPLGRQRVVSTGSA 1328 + G +++ + N AS IAGG + ++NG N +NN G + + T Sbjct: 914 ASGRITINTAALTNDASDIIAGGAL---TINGANGSAASGGVVNNIGYSINRTAIGTAVD 970 Query: 1329 SYTYIKSHTFSADDRRYDDAPYQSQDIVTNFQLDITPTSGAGPNRQNSVRAVASSVSGAS 1388 Y DR Y + ++ ++T N Q S +AV+ S + Sbjct: 971 HY-----------DRHVGHHTYTTLNLT-----EVTALQTIDANIQ-SNQAVSISAQNLN 1013 Query: 1389 GASAGAASIR-----IANLDLTLPNNALFRINAGPAQRFLVQTDPQFVGTHSWLSSDFML 1443 + GAA++ + + ++TLP LF + P QR+LV+TD +F ++L SD+ML Sbjct: 1014 NTTVGAATVPTRAGGVGHANITLPAGGLFTVRPEPGQRYLVETDRRFTDYKTFLGSDYML 1073 Query: 1444 NQLGLTASVRQ--LGDGFYEQQLIQRQVQQITGQRYLAGYSSNEAQYQALMNAGVQVAQS 1501 +L + S+ Q LGDGFYEQ+LI Q+ Q+TG+++L GY++ EAQY+ALM++GV A S Sbjct: 1074 GRLTMDPSMTQKRLGDGFYEQKLINDQIAQLTGKKFLDGYTNAEAQYKALMDSGVASAAS 1133 Query: 1502 QRYTLGVALTDAQIAELKTDIVWLVKQTVTLADGSTXAAGLYPCLQCA 1549 + T G+AL+ Q+A L +DIVW+V VTLADGS A L P + A Sbjct: 1134 FKLTPGIALSADQMAALTSDIVWMVDTEVTLADGSRQHA-LVPTVYLA 1180 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.311 0.127 0.354 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 4,632,711 Number of extensions: 198865 Number of successful extensions: 911 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 729 Number of HSP's successfully gapped: 16 Length of query: 1560 Length of database: 1,619,870 Length adjustment: 98 Effective length of query: 1462 Effective length of database: 1,155,840 Effective search space: 1689838080 Effective search space used: 1689838080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 137 (57.4 bits)