BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0028.p (197 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310003|ref|YP_003774095.1| branched-chain amino acid perme... 353 6e-99 >gi|300310003|ref|YP_003774095.1| branched-chain amino acid permease [Herbaspirillum seropedicae SmR1] Length = 261 Score = 353 bits (905), Expect = 6e-99, Method: Compositional matrix adjust. Identities = 173/192 (90%), Positives = 183/192 (95%) Query: 1 MSLLVYAGSAQFAALPLLAAGVPLLVVFFTAMIVNLRFVIFSAAVAPHFEHLPWYRRVWY 60 MSLLVYAGSAQFA LPLLA G PLLVVFFTAMIVNLRF+IFSAAVAPHFEHLPWYRR+WY Sbjct: 67 MSLLVYAGSAQFAVLPLLAVGTPLLVVFFTAMIVNLRFIIFSAAVAPHFEHLPWYRRIWY 126 Query: 61 GFFNGDISMAFFPQRYPPSTFHQPAGKIGYFAAICYPGWVAWQLGATIGILLGSQIPESW 120 GFFNGDISMAFFPQR+PPSTF QPAGKIGYF+AICYPGW+AWQLGATIGILL SQIPESW Sbjct: 127 GFFNGDISMAFFPQRFPPSTFGQPAGKIGYFSAICYPGWLAWQLGATIGILLASQIPESW 186 Query: 121 NIGFAGTLALIAVMIPMTSSLPALAGVVVSGTVAVLALDLPYRLGLLLAMVLGMAAAMLA 180 NIGFAGTLALIA+MIPMTSSLPALAGVVVSGTVAVLAL+LPYRLGLLLAMVLGM+AAMLA Sbjct: 187 NIGFAGTLALIAIMIPMTSSLPALAGVVVSGTVAVLALELPYRLGLLLAMVLGMSAAMLA 246 Query: 181 DKLLPGSGSDGD 192 DKLLPG + + Sbjct: 247 DKLLPGKDEEEE 258 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.141 0.444 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 587,368 Number of extensions: 24174 Number of successful extensions: 101 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 101 Number of HSP's successfully gapped: 1 Length of query: 197 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 117 Effective length of database: 1,241,070 Effective search space: 145205190 Effective search space used: 145205190 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 128 (53.9 bits)