BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0021.p (522 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309997|ref|YP_003774089.1| Zn-dependent protease [Herbaspi... 904 0.0 gi|300312597|ref|YP_003776689.1| Zn-dependent protease with chap... 89 1e-18 gi|300312643|ref|YP_003776735.1| Zn-dependent protease with chap... 71 2e-13 >gi|300309997|ref|YP_003774089.1| Zn-dependent protease [Herbaspirillum seropedicae SmR1] Length = 552 Score = 904 bits (2336), Expect = 0.0, Method: Compositional matrix adjust. Identities = 462/525 (88%), Positives = 488/525 (92%), Gaps = 11/525 (2%) Query: 1 MQRTVPE---LSRRPLRRLLMRALSATFLLLPAAPLTLLPHPGVAQGLPTLGDTEREGLS 57 MQR VP+ SRRP R+L+ R LSA LLLPAAPLTLLPHPGVAQ LPTLGDTEREGLS Sbjct: 35 MQRYVPDAIACSRRPARKLITRLLSAALLLLPAAPLTLLPHPGVAQSLPTLGDTEREGLS 94 Query: 58 PLMERKLGEEIMRDIRTDRDYVDDGPISEYLNNFGQNLVSIHPEVRGEAGFDFQFFMVRD 117 PLMER+LGEEIMRDIR+DRDYVDDGPISEYLNNFG NLVSIHPEVRGEAGFDFQFFMVRD Sbjct: 95 PLMERRLGEEIMRDIRSDRDYVDDGPISEYLNNFGLNLVSIHPEVRGEAGFDFQFFMVRD 154 Query: 118 PMLNAFALPGGFIGVNSGLVLAAQSESELAGVMSHEIGHVAQRLMDGYIAVGXARMLGQQ 177 PMLNAFALPGGFIGVNSGLVLAAQSESELAGVMSHEIGHVAQR + ARMLGQQ Sbjct: 155 PMLNAFALPGGFIGVNSGLVLAAQSESELAGVMSHEIGHVAQRHI--------ARMLGQQ 206 Query: 178 KQNSMIQLAAIVLGALAGVSKAGGDAAMATMMGGTGLAVQRQLNFSRDAEREADRIGLQI 237 KQNSMIQLAAIVLGALAGVSKAGGDAAMATMMGGTGLAVQRQLNFSRDAEREADRIGLQI Sbjct: 207 KQNSMIQLAAIVLGALAGVSKAGGDAAMATMMGGTGLAVQRQLNFSRDAEREADRIGLQI 266 Query: 238 MRDGGFDPSGMVTFFGRLQTASRNYSDAVPPYLMTHPLTTERIADIQARIRDQRYKQRVD 297 MRDGGFDPSGMVTFFGRLQ ASRNYSDAVPPYLMTHPLTTERIADI+ARIRDQRYKQRVD Sbjct: 267 MRDGGFDPSGMVTFFGRLQAASRNYSDAVPPYLMTHPLTTERIADIEARIRDQRYKQRVD 326 Query: 298 NPEFQLARARTQVLQNDSVQGLRDNVAKFTDQAKGNTKLMTAAAKYGLAYAYYRQNRFEQ 357 NPEFQL RARTQVLQND+VQGLR+N KF QA+ NTKL TAAAKYGLAY YYRQN+FEQ Sbjct: 327 NPEFQLVRARTQVLQNDTVQGLRENAEKFIAQARQNTKLQTAAAKYGLAYVYYRQNKFEQ 386 Query: 358 AEAALKEAQEAAIPMKTGIFFGSLDIDIKIARKQTQQALQVAESMRQQYPISRSVARQYA 417 AE ALKEAQEAAIPMKTGI FGSLDIDIKIARKQ+ QA+Q+A+++R+Q+PISR++ARQYA Sbjct: 387 AETALKEAQEAAIPMKTGIMFGSLDIDIKIARKQSSQAVQLADALRKQFPISRTIARQYA 446 Query: 418 DALIAANRYDQAVSYLRDQAQLYRSDAQVQQLLAKVYAAQGKQALQHLALAESYALNGSL 477 DALIAA R D+AV+YLRDQAQLYRSDAQVQQLLAK YA+QGKQALQHLALAESYALNGSL Sbjct: 447 DALIAARRTDEAVAYLRDQAQLYRSDAQVQQLLAKAYASQGKQALQHLALAESYALNGSL 506 Query: 478 LAAIDQLGIARKAPDATFYDQSQIDAREREWKARWKDEQKELKDR 522 LAAIDQLGIAR+APDATFYDQSQIDAREREWK RWKDEQKE+KDR Sbjct: 507 LAAIDQLGIARRAPDATFYDQSQIDAREREWKERWKDEQKEMKDR 551 >gi|300312597|ref|YP_003776689.1| Zn-dependent protease with chaperone function protein [Herbaspirillum seropedicae SmR1] Length = 273 Score = 88.6 bits (218), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 66/201 (32%), Positives = 98/201 (48%), Gaps = 28/201 (13%) Query: 108 FDFQFFMVRDPMLNAFALPGGFIGVNSGLVLAAQ-SESELAGVMSHEIGHVAQRLMDGYI 166 ++++ ++ NA+ +PGG I V +GL+ + S+ ELA VM HEI H + Sbjct: 100 WNWEVNVLSSDETNAWCMPGGKIAVYTGLIEKLKISDDELAAVMGHEIAHALREH----- 154 Query: 167 AVGXARMLGQQKQNSMIQLAAIVLGALAGVSKAGGDAAMATMMGGTGLAVQRQLNFSRDA 226 R Q NS I + A +LG L V K GG +MG L SR Sbjct: 155 --ARERASQQVVANSAISIGAALLG-LGDVGKQGGQYLYMGLMG---------LPNSRAN 202 Query: 227 EREADRIGLQIMRDGGFDPSGMVTFFGRLQTASRNYSDAVPPYLMTHPLTTERIADIQAR 286 E EADRIG+++ GG+DP VT + ++ + + ++ THP ++ERIAD+ Sbjct: 203 ETEADRIGVELAARGGYDPKAAVTLWQKMASLG---GEEPMKFMSTHPSSSERIADLTV- 258 Query: 287 IRDQRYKQRVDNPEFQLARAR 307 Y QRV+ P +Q R R Sbjct: 259 -----YAQRVE-PLYQQTRRR 273 >gi|300312643|ref|YP_003776735.1| Zn-dependent protease with chaperone function transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 329 Score = 71.2 bits (173), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 54/163 (33%), Positives = 77/163 (47%), Gaps = 25/163 (15%) Query: 121 NAFALPGGFIGVNSGLVLAAQSESELAGVMSHEIGHVAQRLMDGYIAVGXARMLGQQKQN 180 NAFALP G I V +V + L GV++HE+GHV +R ++ + Q+ Sbjct: 188 NAFALPSGQIVVTDEIVRLLDDDDALMGVLAHELGHVHRR-----------HLMRRLIQS 236 Query: 181 SMIQLAAIVLGALAGVSKAGGDAAMATMMGGTGLAVQRQLNFSRDAEREADRIGLQIMRD 240 S I A VL VS A +A + V L +SRD EREAD + + + Sbjct: 237 SAIAATATVL--FGDVS-----AVLANIP-----TVLLDLKYSRDIEREADDYAIAMFKA 284 Query: 241 GGFDPSGMVTFFGRLQTASRNYSDAVPPYLMTHPLTTERIADI 283 G + F +L++A ++ D PYL +HP ERIA I Sbjct: 285 NGLPRQKLALVFEKLESAGKD--DPASPYLSSHPANDERIAHI 325 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,267,789 Number of extensions: 48071 Number of successful extensions: 216 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 210 Number of HSP's successfully gapped: 3 Length of query: 522 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 432 Effective length of database: 1,193,720 Effective search space: 515687040 Effective search space used: 515687040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)